BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	946,689	A→G	T144A (ACG→GCG)	b0899 →	putative transport

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	946,689	0	A	G	99.4%	584.5 / NA	172	T144A (ACG→GCG)	b0899	putative transport
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (97/74); total (97/75)
Fisher's exact test for biased strand distribution p-value = 4.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 181 total aligned reads displayed.

CTCTATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACACGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCAATTCTG  >  NC_000913/946599‑946778

ctctATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACAcgcg                                                                                         >  1:4804941/1‑93 (MQ=255)

 tctATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGa                                                                                        <  2:4706522/93‑1 (MQ=255)

  ctATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTCCCTGGATTAAACACGCGAt                                                                                       <  1:3546859/93‑1 (MQ=255)

  ctATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGAt                                                                                       <  2:4915648/93‑1 (MQ=255)

   tATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATg                                                                                      >  1:2727828/1‑93 (MQ=255)

    ttttCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGCGTCAGTACCTGGATTAAACTCGCGATgg                                                                                     <  2:795938/92‑1 (MQ=255)

    aTTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGCAGGGCGGGGTCAGTACCTGGATTAAACACGCGATgg                                                                                     >  1:876122/1‑93 (MQ=255)

    aTTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATgg                                                                                     >  1:5355155/1‑93 (MQ=255)

      ttCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGa                                                                                   <  2:2537926/93‑1 (MQ=255)

       tCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGAc                                                                                  >  1:1196487/1‑93 (MQ=255)

        cacaCCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGAcc                                                                                 >  1:2494851/1‑93 (MQ=255)

         acacCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGAccc                                                                                <  1:2811231/93‑1 (MQ=255)

         acacCTTATGCGCTAATTGGTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGAccc                                                                                >  1:3131714/1‑93 (MQ=255)

            ccTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGa                                                                             <  2:4137868/93‑1 (MQ=255)

             cTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGAc                                                                            <  2:5167006/93‑1 (MQ=255)

             cTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGAc                                                                            <  1:1261791/93‑1 (MQ=255)

             cTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGAc                                                                            >  1:4858467/1‑93 (MQ=255)

             cTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGAc                                                                            >  1:3868713/1‑93 (MQ=255)

             cTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAAGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCAGAc                                                                            >  1:3139385/1‑93 (MQ=255)

              ttATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACt                                                                           <  1:1855453/93‑1 (MQ=255)

               tATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTg                                                                          >  1:6065716/1‑93 (MQ=255)

                    gcTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTa                                                                     <  1:1288356/93‑1 (MQ=255)

                    gcTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAAAACCCGATGGGACCCGGACTGCCTTa                                                                     >  1:4312337/1‑93 (MQ=255)

                       aaTTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTAtct                                                                  <  2:1422753/93‑1 (MQ=255)

                        aTTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTAtctc                                                                 >  2:1443271/1‑93 (MQ=255)

                        aTTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTAtctc                                                                 >  2:3043695/1‑93 (MQ=255)

                         ttgttgGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTcg                                                                >  2:511512/1‑93 (MQ=255)

                          tgttgGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTcgc                                                               >  1:4035525/1‑93 (MQ=255)

                          tgttgGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTcgc                                                               <  2:5276843/93‑1 (MQ=255)

                          tgttgGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTcgc                                                               <  1:557179/93‑1 (MQ=255)

                            ttgGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTcgctg                                                             <  2:6245704/93‑3 (MQ=255)

                            ttgGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTcgccg                                                             <  2:418475/93‑1 (MQ=255)

                             tgGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTcgccgc                                                            <  1:3923687/93‑1 (MQ=255)

                               gTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGt                                                          <  1:4579873/93‑1 (MQ=255)

                                 cAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTgg                                                        >  2:2232075/1‑93 (MQ=255)

                                 cAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTgg                                                        <  1:5575122/93‑1 (MQ=255)

                                  aGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGa                                                       >  1:4602112/1‑93 (MQ=255)

                                  aGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGa                                                       >  1:5905220/1‑93 (MQ=255)

                                   gTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGAc                                                      >  2:2982365/1‑93 (MQ=255)

                                   gTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGAc                                                      >  2:1320120/1‑93 (MQ=255)

                                   gTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGAc                                                      >  1:4219367/1‑93 (MQ=255)

                                    ttAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGAcc                                                     >  2:6037456/1‑93 (MQ=255)

                                     tAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCt                                                    <  1:603004/93‑1 (MQ=255)

                                     tAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCt                                                    <  1:300717/93‑1 (MQ=255)

                                      aGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCtc                                                   >  2:5480444/1‑92 (MQ=255)

                                      aGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTa                                                   >  2:2162408/1‑93 (MQ=255)

                                       ggCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTAc                                                  >  2:6306929/1‑93 (MQ=255)

                                        gCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACt                                                 >  2:229405/1‑93 (MQ=255)

                                         cTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTg                                                <  2:3614179/93‑1 (MQ=255)

                                         cTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTg                                                <  2:176961/93‑1 (MQ=255)

                                          tCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTgg                                               >  1:3648280/1‑93 (MQ=255)

                                          tCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTgg                                               >  2:4415076/1‑93 (MQ=255)

                                           cGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTggg                                              >  1:3759667/1‑93 (MQ=255)

                                            gACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGggt                                             >  2:5207613/1‑93 (MQ=255)

                                            gACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGggt                                             <  2:569245/93‑1 (MQ=255)

                                             aCCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGggtg                                            <  1:970882/93‑1 (MQ=255)

                                                ttCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGgtggtg                                         <  2:2661572/93‑1 (MQ=255)

                                                 tCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGc                                        >  2:5207926/1‑93 (MQ=255)

                                                  cAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCa                                       <  2:1214018/93‑1 (MQ=255)

                                                   aaaGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCat                                      <  2:2177742/93‑1 (MQ=255)

                                                     aGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCatat                                    <  1:1045274/93‑1 (MQ=255)

                                                     aGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCatat                                    <  2:432508/93‑1 (MQ=255)

                                                     aGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCatat                                    <  2:335142/93‑1 (MQ=255)

                                                     aGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCatat                                    <  1:3781737/93‑1 (MQ=255)

                                                       aTGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTc                                  <  2:5616108/93‑1 (MQ=255)

                                                        tGGGAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTcc                                 <  2:3082908/93‑1 (MQ=255)

                                                         tggAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTccc                                <  2:3160584/92‑1 (MQ=255)

                                                         gggAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTccc                                <  1:5820011/93‑1 (MQ=255)

                                                          ggAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCt                               >  2:3480060/1‑93 (MQ=255)

                                                           gAAGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGGGCCTATTCCCTa                              >  2:1671183/1‑93 (MQ=255)

                                                             aGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATc                            <  1:656751/93‑1 (MQ=255)

                                                             aGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATc                            <  2:6134298/93‑1 (MQ=255)

                                                             aGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATc                            >  2:885351/1‑93 (MQ=255)

                                                             aGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATc                            <  1:4712455/93‑1 (MQ=255)

                                                             aGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATc                            >  2:2990175/1‑93 (MQ=255)

                                                             aGGGCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGGGCATATTCCCTATc                            >  1:5126669/1‑93 (MQ=255)

                                                                gCGGGGTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTgg                         <  1:1471383/93‑1 (MQ=255)

                                                                  ggggTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGca                       >  2:3755321/1‑93 (MQ=255)

                                                                   gggTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGcac                      >  1:3281031/1‑93 (MQ=255)

                                                                   gggTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGcac                      >  1:6263091/1‑93 (MQ=255)

                                                                   gggTCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGcac                      >  1:5214917/1‑93 (MQ=255)

                                                                      tCAGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACaaa                   >  2:4817528/1‑93 (MQ=255)

                                                                        aGTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACaaaaa                 >  2:1594745/1‑93 (MQ=255)

                                                                         gTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAAc                >  1:1632510/1‑93 (MQ=255)

                                                                         gTACCTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGCGGTGCATATTCGCTATCTGCTACAAAAAc                >  1:967008/1‑93 (MQ=255)

                                                                         ctACCTGGATTAAACACGCGATGGGCCCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAAc                <  1:191050/92‑1 (MQ=255)

                                                                            ccTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAAcccc             <  1:5939853/93‑1 (MQ=255)

                                                                             cTGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCa            <  1:2656654/93‑1 (MQ=255)

                                                                              tGGATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAg           <  2:1737130/93‑1 (MQ=255)

                                                                               ggATTAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAgg          <  2:728197/93‑1 (MQ=255)

                                                                                  ttAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCaa       >  2:580064/1‑93 (MQ=255)

                                                                                  ttAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCaa       >  2:77886/1‑93 (MQ=255)

                                                                                  ttAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCaa       >  1:2087635/1‑93 (MQ=255)

                                                                                   tAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCAAt      <  2:4550548/93‑1 (MQ=255)

                                                                                   tAAACACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCAAt      >  1:3024185/1‑93 (MQ=255)

                                                                                     aaCACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCAATTc    >  1:444502/1‑93 (MQ=255)

                                                                                     aaCACGCGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCAATTc    >  2:6451498/1‑93 (MQ=255)

                                                                                      acacgcGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCAATTCt   >  2:4662145/1‑93 (MQ=255)

                                                                                      acacgcGATGGGACCCGGACTGGCTTATCTCGCCGCGGGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCTCAGGCAAGTCt   >  2:3991320/1‑93 (MQ=255)

                                                                                       cacgcGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCAATTCTg  >  1:6116271/1‑93 (MQ=255)

CTCTATTTCACACCTTATGCGCTAATTGTTGGTCAGTTAGGCTCGACCTTCAAAGATGGGAAGGGCGGGGTCAGTACCTGGATTAAACACACGATGGGACCCGGACTGGCTTATCTCGCCGCGTGGACCTACTGGGTGGTGCATATTCCCTATCTGGCACAAAAACCCCAGGCAATTCTG  >  NC_000913/946599‑946778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`