BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,156,390	A→G	T131A (ACC→GCC)	ycfH →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,156,390	0	A	G	99.5%	697.6 / NA	201	T131A (ACC→GCC)	ycfH	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (115/85); minor base T (0/1); total (115/86)
Fisher's exact test for biased strand distribution p-value = 4.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 206 total aligned reads displayed.

TACACGCCGGAAACTAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGAC  >  NC_000913/1156300‑1156479

taCACGCCGGAAACTAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGcc                                                                                         <  2:2098434/93‑1 (MQ=255)

  cacGCCGGAAACTAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCg                                                                                       >  2:647398/1‑93 (MQ=255)

   acGCCGGAAACTAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGt                                                                                      >  1:697458/1‑93 (MQ=255)

   acGCCGGAAACTAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGt                                                                                      <  1:1669697/93‑1 (MQ=255)

    cGCCGGAAACTAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTg                                                                                     <  2:1283468/93‑1 (MQ=255)

     gCCGGAAACTAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGa                                                                                    >  2:3416399/1‑93 (MQ=255)

      ccGGAAACTAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGAc                                                                                   <  2:1375157/93‑1 (MQ=255)

      ccGGAAACTAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGAc                                                                                   <  1:2915443/93‑1 (MQ=255)

        ggAAACTAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGc                                                                                 <  1:5234070/93‑1 (MQ=255)

         gAAACTAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGcc                                                                                >  1:4499252/1‑93 (MQ=255)

           aaCTAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCg                                                                              >  2:872825/1‑93 (MQ=255)

           aaCTAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCg                                                                              >  2:3529608/1‑93 (MQ=255)

            aCTAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGt                                                                             >  2:5709538/1‑93 (MQ=255)

             cTAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTg                                                                            <  2:357018/93‑1 (MQ=255)

               aaaGTACGTCAGCAAGAGTCTTTCATCCATAATTTCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGcc                                                                          <  2:1772689/93‑1 (MQ=255)

               aaaGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGcc                                                                          >  1:2122624/1‑93 (MQ=255)

               aaaGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGcc                                                                          <  1:1428218/93‑1 (MQ=255)

               aaaGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGcc                                                                          <  1:3868814/93‑1 (MQ=255)

                aaGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCg                                                                         >  2:6274922/1‑93 (MQ=255)

                  gTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGAt                                                                       >  1:5010252/1‑93 (MQ=255)

                  gTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGAt                                                                       >  1:6115569/1‑93 (MQ=255)

                  gTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGAt                                                                       >  1:251590/1‑93 (MQ=255)

                    aCGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATAc                                                                     >  2:82900/1‑93 (MQ=255)

                    aCGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATAc                                                                     >  1:127531/1‑93 (MQ=255)

                    aCGTCAGCAAGAGCCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATAc                                                                     <  1:2307997/93‑1 (MQ=255)

                      gTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGc                                                                   >  1:6401594/1‑93 (MQ=255)

                      gTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGc                                                                   <  2:228340/93‑1 (MQ=255)

                         aGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTgg                                                                >  1:4797834/1‑93 (MQ=255)

                          gCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGc                                                               >  1:135173/1‑93 (MQ=255)

                          gCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGc                                                               >  1:3227003/1‑93 (MQ=255)

                            aaGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCaa                                                             >  2:3656577/1‑93 (MQ=255)

                            aaGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCaa                                                             <  2:1541159/93‑1 (MQ=255)

                             agagTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAAt                                                            >  2:5659902/1‑93 (MQ=255)

                             agagTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAAt                                                            >  2:3937784/1‑93 (MQ=255)

                             agagTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAAt                                                            >  2:2752299/1‑93 (MQ=255)

                             agagTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAAt                                                            >  2:5216421/1‑93 (MQ=255)

                              gagTCCTTCATCCATCATATCCAGATTGGTCGTGAACGGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAAtt                                                           <  2:6238403/93‑1 (MQ=255)

                               agTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTc                                                          <  1:3031635/93‑1 (MQ=255)

                                gTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCt                                                         <  1:5301516/93‑1 (MQ=255)

                                gTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCt                                                         >  2:2616941/1‑93 (MQ=255)

                                gTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCt                                                         >  1:4893298/1‑93 (MQ=255)

                                  ccTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTgc                                                       >  1:247385/1‑93 (MQ=255)

                                     tCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGcgcg                                                    >  1:2853810/1‑93 (MQ=255)

                                      catccatcATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCga                                                   >  1:5132701/1‑93 (MQ=255)

                                      catccatcATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCga                                                   >  1:228607/1‑93 (MQ=255)

                                       atccatcATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGcgcgca                                                  >  2:5445842/1‑91 (MQ=255)

                                        tccatcATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCgaag                                                 <  2:3924986/93‑1 (MQ=255)

                                         ccatcATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCgaaga                                                >  1:170147/1‑93 (MQ=255)

                                         ccatcATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCgaaga                                                <  1:2754829/93‑1 (MQ=255)

                                         ccatcATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCgaaga                                                >  1:1078910/1‑93 (MQ=255)

                                          catcatATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCgaagaa                                               >  1:1537203/1‑93 (MQ=255)

                                          catcatATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCgaagaa                                               <  1:2583249/93‑1 (MQ=255)

                                            tcatATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGaaaa                                             >  2:488392/1‑93 (MQ=255)

                                             catATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGaaaaa                                            >  2:1528066/1‑93 (MQ=255)

                                              atatCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAg                                           <  2:5120816/93‑1 (MQ=255)

                                                atCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTg                                         >  2:5848536/1‑93 (MQ=255)

                                                atCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTg                                         >  1:5609892/1‑93 (MQ=255)

                                                 accAGATTGGTCGTAAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGa                                        <  1:5542529/92‑1 (MQ=255)

                                                   cAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACg                                      <  1:3048706/93‑1 (MQ=255)

                                                   cAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACg                                      <  1:3651528/93‑1 (MQ=255)

                                                     gATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGa                                    >  2:2669384/1‑93 (MQ=255)

                                                     gATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGa                                    <  2:639322/93‑1 (MQ=255)

                                                      aTTGGTCGTGAACTGACCAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGAt                                   <  2:4038665/93‑1 (MQ=255)

                                                      aTTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGAt                                   <  2:3995577/93‑1 (MQ=255)

                                                      aTTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGAt                                   <  1:38869/93‑1 (MQ=255)

                                                         ggTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGc                                <  2:269650/93‑1 (MQ=255)

                                                         ggTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGAGACGGATTGc                                >  2:3115592/1‑93 (MQ=255)

                                                          gTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCg                               <  1:6389870/93‑1 (MQ=255)

                                                          gTCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCg                               >  2:5598786/1‑93 (MQ=255)

                                                           tCGTGAACTGTACAAGCCGGTTATCGTCCATGCGCTTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCgg                              <  1:3162315/93‑1 (MQ=255)

                                                           tCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCgg                              <  1:1957346/93‑1 (MQ=255)

                                                           tCGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCgg                              <  2:4108190/93‑1 (MQ=255)

                                                            cGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGt                             <  2:202549/93‑1 (MQ=255)

                                                            cGTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGt                             <  2:4159419/93‑1 (MQ=255)

                                                             gTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTg                            >  1:3415957/1‑93 (MQ=255)

                                                             gTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTg                            >  1:3136185/1‑93 (MQ=255)

                                                             gTGAACTGAACAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTg                            <  2:1247209/93‑1 (MQ=255)

                                                               gaactgaacAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGc                          >  1:1225987/1‑93 (MQ=255)

                                                                aactgaacAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCg                         <  1:1891683/93‑1 (MQ=255)

                                                                aactgaacAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCg                         <  1:3334854/93‑1 (MQ=255)

                                                                aactgaacAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCg                         <  2:1130266/93‑1 (MQ=255)

                                                                aactgaacAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCg                         <  2:5682244/93‑1 (MQ=255)

                                                                 actgaacAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGt                        <  2:682604/93‑1 (MQ=255)

                                                                   tgaacAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGAGACGGATTGCGGTGGCGtac                      >  1:5040232/1‑93 (MQ=255)

                                                                     aacAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGtacga                    >  1:6223577/1‑91 (MQ=255)

                                                                      acAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGtactac                   >  2:4303549/1‑93 (MQ=255)

                                                                       cAAGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTaca                  >  1:582830/1‑93 (MQ=255)

                                                                         aGCCGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACt                >  1:4627839/1‑93 (MQ=255)

                                                                           ccGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGt              <  2:4697168/93‑1 (MQ=255)

                                                                           ccGGTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGt              <  2:1481896/93‑1 (MQ=255)

                                                                             ggTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCACGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGttt            <  2:4525474/93‑1 (MQ=255)

                                                                              gTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGtttt           <  2:1094065/93‑1 (MQ=255)

                                                                              gTTATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGtttt           >  2:940789/1‑93 (MQ=255)

                                                                                tATCGTCCATTCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTAc         <  2:4335249/93‑1 (MQ=255)

                                                                                tATCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGCTTGCGGTGGCGTCCTACACTGTTTTAc         >  1:4014366/1‑93 (MQ=255)

                                                                                 aTCGTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACa        >  2:5820354/1‑93 (MQ=255)

                                                                                    gTCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACagag     <  2:5244244/93‑1 (MQ=255)

                                                                                     tCCATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAgg    >  2:555873/1‑93 (MQ=255)

                                                                                      ccATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGa   >  1:5746512/1‑93 (MQ=255)

                                                                                       cATGCCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGAc  >  2:2858715/1‑93 (MQ=255)

TACACGCCGGAAACTAAAGTACGTCAGCAAGAGTCCTTCATCCATCATATCCAGATTGGTCGTGAACTGAACAAGCCGGTTATCGTCCATACCCGTGACGCCCGTGCCGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGAC  >  NC_000913/1156300‑1156479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`