BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,213,846	A→G	F285L (TTT→CTT)	b1163 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,213,846	0	A	G	99.5%	719.7 / ‑5.5	206	F285L (TTT→CTT)	b1163	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (105/100); total (106/100)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 209 total aligned reads displayed.

ACAAACCAGCAAAACCTGCGCCAAAATGATCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAACCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTATTTCATTAAGTAAAAAAGAGA  >  NC_000913/1213756‑1213935

  aaaCCAGCAAAACCTGCGCCAAAATGATCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCga                                                                                       >  1:6439627/1‑93 (MQ=255)

    aCCAGCAAAACCTGCGCCAAAATGATCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCgaga                                                                                     <  1:3193519/93‑1 (MQ=255)

    aCCAGCAAAACCTGCGCCAAAATGATCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCgaga                                                                                     >  2:4222059/1‑93 (MQ=255)

    aCCAGCAAAACCTGCGCCAAAATGATCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCgaga                                                                                     >  2:2412283/1‑93 (MQ=255)

     ccAGCAAAACCTGCGCCAAAATGATCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGat                                                                                    >  2:3406722/1‑93 (MQ=255)

     ccAGCAAAACCTGCGCCAAAATGATCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGat                                                                                    >  2:6263713/1‑93 (MQ=255)

      cAGCAAAACCTGCGCCAAAATGATCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGata                                                                                   <  2:5281386/93‑1 (MQ=255)

      cAGCAAAACCTGCGCCAAAATGATCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGata                                                                                   <  1:3097833/93‑1 (MQ=255)

      cAGCAAAACCTGCGCCAAAATGATCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGata                                                                                   >  2:5262209/1‑93 (MQ=255)

       aGCAAAACCTGCGCCAAAATGATCATTTGCTACACTGATCCCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGatat                                                                                  <  1:2651684/93‑1 (MQ=255)

        gCAAAACCTGCGCCAAAATGATCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATg                                                                                 <  1:4450343/93‑1 (MQ=255)

         cAAAACCTGCGCCAAAATGATCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGa                                                                                >  1:2746051/1‑93 (MQ=255)

          aaaaCCTGCGCCAAAATGATCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGAc                                                                               >  2:1570196/1‑93 (MQ=255)

            aaCCTGCGCCAAAATGATCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACtt                                                                             <  2:2468565/93‑1 (MQ=255)

                tGCGCCAAAATGATCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACt                                                                         <  1:2029768/93‑1 (MQ=255)

                tGCGCCAAAATGATCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACt                                                                         <  2:3943719/93‑1 (MQ=255)

                  cgcCAAAATGATCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACttt                                                                       <  2:400430/93‑1 (MQ=255)

                   gcCAAAATGATCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTc                                                                      <  2:1771412/93‑1 (MQ=255)

                   gcCAAAATGATCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTc                                                                      <  1:3637208/93‑1 (MQ=255)

                    ccAAAATGATCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCa                                                                     <  2:3097659/93‑1 (MQ=255)

                     cAAAATGATCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAg                                                                    <  2:1985023/93‑1 (MQ=255)

                      aaaaTGATCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGt                                                                   >  1:5437517/1‑93 (MQ=255)

                      aaaaTGATCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGt                                                                   >  1:1076409/1‑93 (MQ=255)

                      aaaaTGATCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGt                                                                   >  2:269758/1‑93 (MQ=255)

                       aaaTGATCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTa                                                                  <  2:2382594/93‑1 (MQ=255)

                       aaaTGATCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTa                                                                  <  1:5596041/93‑1 (MQ=255)

                         aTGATCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTaaa                                                                >  1:924306/1‑93 (MQ=255)

                         aTGATCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTaaa                                                                >  2:1161494/1‑93 (MQ=255)

                          agATCCATTGCTACCCTGAAACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAAt                                                               <  2:3746522/92‑1 (MQ=255)

                           gATCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAAtt                                                              <  1:3721630/93‑1 (MQ=255)

                            aTCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTc                                                             <  2:6093520/93‑1 (MQ=255)

                               aaTTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAAc                                                          >  2:4483678/1‑93 (MQ=255)

                                aTTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACg                                                         >  2:3845210/1‑93 (MQ=255)

                                aTTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACg                                                         >  2:4897549/1‑93 (MQ=255)

                                  tGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACgat                                                       <  1:1736797/93‑1 (MQ=255)

                                     tACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACgatgat                                                    <  2:6199676/93‑1 (MQ=255)

                                      acacTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGAtt                                                   >  2:163855/1‑93 (MQ=255)

                                      acacTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGAtt                                                   >  2:4355723/1‑93 (MQ=255)

                                      acacTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGAtt                                                   >  1:729003/1‑93 (MQ=255)

                                      acacTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGAtt                                                   >  2:4657959/1‑93 (MQ=255)

                                      acacTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGAtt                                                   >  1:5885671/1‑93 (MQ=255)

                                      acacTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGAtt                                                   >  1:5654296/1‑93 (MQ=255)

                                      acacTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGAtt                                                   >  2:6191594/1‑93 (MQ=255)

                                       cacTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCATGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGAttt                                                  <  2:4534230/93‑1 (MQ=255)

                                       cacTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGAttt                                                  >  2:2586320/1‑93 (MQ=255)

                                       cacTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGAttt                                                  >  1:5380606/1‑93 (MQ=255)

                                         cTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGt                                                <  2:2833310/93‑1 (MQ=255)

                                          tGATGCCAGCAGCCTTTAGCGATTTAATCGCGTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGtt                                               <  1:3027655/93‑1 (MQ=255)

                                          tGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGtt                                               <  2:4113164/93‑1 (MQ=255)

                                             tACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAg                                            >  1:2407208/1‑93 (MQ=255)

                                             tACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAg                                            >  1:4193108/1‑93 (MQ=255)

                                              aCCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAgg                                           >  1:3858565/1‑93 (MQ=255)

                                              aCCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAgg                                           <  2:4732746/93‑1 (MQ=255)

                                                cagcagCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAGGCa                                         >  1:33435/1‑93 (MQ=255)

                                                  gcagcCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAGGCAcc                                       >  1:2251161/1‑93 (MQ=255)

                                                   cagcCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAGGCACCa                                      <  1:3450455/93‑1 (MQ=255)

                                                   cagcCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAGGCACCa                                      >  1:4760477/1‑93 (MQ=255)

                                                    agcCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAGGCACCag                                     >  2:3179866/1‑93 (MQ=255)

                                                     gcCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAGGCACCaga                                    >  2:5980759/1‑93 (MQ=255)

                                                     gcCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAGGCACCaga                                    >  2:628546/1‑93 (MQ=255)

                                                     gcCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAGGCACCaga                                    <  1:1391497/93‑1 (MQ=255)

                                                      ccTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAGGCACCagag                                   >  2:4246563/1‑93 (MQ=255)

                                                       cTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAGGCACCAGAGc                                  <  1:5424095/93‑1 (MQ=255)

                                                         ttAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAGGCACCAGAGCAt                                >  1:4080769/1‑93 (MQ=255)

                                                         ttAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAGGCACCAGAGCAt                                >  2:1520851/1‑93 (MQ=255)

                                                          tAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAGGCACCAGAGCAtt                               >  1:298806/1‑93 (MQ=255)

                                                          tAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAGGCACCAGAGCAtt                               >  2:1011048/1‑93 (MQ=255)

                                                          tAGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAGGCACCAGAGCAtt                               <  1:4831700/93‑1 (MQ=255)

                                                           aGCGATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTg                              <  1:1477796/93‑1 (MQ=255)

                                                           aGCGATTTAATCGCTCCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTg                              <  1:3981781/93‑1 (MQ=255)

                                                              gATTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTGGcc                           <  2:770359/93‑1 (MQ=255)

                                                              aaTTTAATCTCTGCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTGGcc                           <  1:1124830/92‑1 (MQ=255)

                                                               aTTTAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTGGCCt                          >  1:2350729/1‑93 (MQ=255)

                                                                tttAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTGGCCtt                         >  2:206411/1‑93 (MQ=255)

                                                                  tAATCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTa                       >  1:5213087/1‑93 (MQ=255)

                                                                   aaTCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTAt                      <  2:1407869/93‑1 (MQ=255)

                                                                   aaTCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTAt                      <  2:5688181/93‑1 (MQ=255)

                                                                   aaTCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTAt                      >  2:1222046/1‑93 (MQ=255)

                                                                    accGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTAAACGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTAtt                     <  1:2929596/91‑1 (MQ=255)

                                                                    aTCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTAtt                     <  1:3786217/93‑1 (MQ=255)

                                                                    aTCGCTGCGGCAACCTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTAtt                     <  2:5113866/93‑1 (MQ=255)

                                                                     tCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTAttt                    <  1:1825876/93‑1 (MQ=255)

                                                                     tCGCTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTAttt                    <  1:3617018/93‑1 (MQ=255)

                                                                       gCTTCGGCAAACTCATCAAGCCGCGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTATTTCa                  >  1:5521866/1‑93 (MQ=255)

                                                                        cTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTATTTCAt                 >  2:3593300/1‑93 (MQ=255)

                                                                        cTTCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTATTTCAt                 >  1:6178019/1‑93 (MQ=255)

                                                                         ttCGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTATTTCAtt                >  2:324106/1‑93 (MQ=255)

                                                                           cGGCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTATTTCATTaa              <  2:5811759/93‑1 (MQ=255)

                                                                            ggCAAACTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTATTTCATTAAg             <  2:3469302/93‑1 (MQ=255)

                                                                               aaaCTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTATTTCATTAAGTaa          >  2:2943598/1‑93 (MQ=255)

                                                                               aaaCTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTATTTCATTAAGTaa          >  2:636268/1‑93 (MQ=255)

                                                                                 aCTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTGTCCTTTATTTCATTAAGTaaaa        <  2:4814238/93‑1 (MQ=255)

                                                                                  cTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTATTTCATTAAGTaaaaa       <  2:3750947/93‑1 (MQ=255)

                                                                                  cTCATCAAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTATTTCATTAAGTaaaaa       <  1:1595574/93‑1 (MQ=255)

                                                                                    catcaAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTATTTCATTAAGTAAAAAag     <  1:6263713/93‑1 (MQ=255)

                                                                                    catcaAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTATTTCATTAAGTAAAAAag     >  2:3141312/1‑93 (MQ=255)

                                                                                     atcaAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTATTTCATTAAGTAAAAAaga    <  2:5547459/93‑1 (MQ=255)

                                                                                     atcaAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTATTTCATTAAGTAAAAAaga    >  2:4366939/1‑93 (MQ=255)

                                                                                     atcaAGCCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTATTTCATTAAGTAAAAAaga    <  1:3243079/93‑1 (MQ=255)

                                                                                       caAGCCGAGATATGACTTCGCTTTCAGTAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTATTTCATTAAGTAAAAAAgaga  <  1:1507574/93‑1 (MQ=255)

ACAAACCAGCAAAACCTGCGCCAAAATGATCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAACCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTATTTCATTAAGTAAAAAAGAGA  >  NC_000913/1213756‑1213935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`