Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,329,903 A→G S278G (AGT→GGT)  topA → DNA topoisomerase type I omega protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,329,9030AG99.7% 1026.2 / NA 293S278G (AGT→GGT) topADNA topoisomerase type I omega protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (159/133);  total (159/134)
Fisher's exact test for biased strand distribution p-value = 4.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 299 total aligned reads displayed.

CCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGCGTGAAAAAAACCATGATGATG  >  NC_000913/1329813‑1329992
                                                                                          |                                                                                         
 cgGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTa                                                                                        >  1:1139826/1‑93 (MQ=255)
  ggTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTaa                                                                                       >  1:5656908/1‑93 (MQ=255)
  ggTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTaa                                                                                       >  2:2023068/1‑93 (MQ=255)
   gTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTaaa                                                                                      <  2:1306334/93‑1 (MQ=255)
      aacaaAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCt                                                                                   >  2:240775/1‑93 (MQ=255)
      aacaaAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCt                                                                                   <  2:682289/93‑1 (MQ=255)
       acaaAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTg                                                                                  >  2:5359793/1‑93 (MQ=255)
       acaaAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTg                                                                                  >  1:2064284/1‑93 (MQ=255)
        caaAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTgg                                                                                 >  2:2548806/1‑93 (MQ=255)
        caaAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTgg                                                                                 >  1:3127765/1‑93 (MQ=255)
        caaAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTgg                                                                                 <  1:5291963/93‑1 (MQ=255)
         aaaGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGgc                                                                                <  2:4400138/93‑1 (MQ=255)
         aaaGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGgc                                                                                <  1:4403253/93‑1 (MQ=255)
         aaaGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGgc                                                                                <  1:3229464/93‑1 (MQ=255)
         aaaGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGgc                                                                                >  1:1548316/1‑93 (MQ=255)
          aaGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGgcg                                                                               <  2:5294156/93‑1 (MQ=255)
          aaGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGgcg                                                                               >  2:64193/1‑93 (MQ=255)
              aCAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTGCAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTcc                                                                           <  2:2663150/93‑1 (MQ=255)
              aCAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTcc                                                                           <  2:3548025/93‑1 (MQ=255)
              aCAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTcc                                                                           >  2:6238895/1‑93 (MQ=255)
                aaaCTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCtt                                                                         >  2:4060357/1‑93 (MQ=255)
                 aaCTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCttt                                                                        <  1:53416/93‑1 (MQ=255)
                 aaCTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCttt                                                                        <  2:1237935/93‑1 (MQ=255)
                  aCTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCtttt                                                                       >  1:3898079/1‑93 (MQ=255)
                    tCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTttat                                                                     <  1:1642563/93‑1 (MQ=255)
                    tCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTttat                                                                     >  2:5577828/1‑93 (MQ=255)
                     cAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTttatt                                                                    <  1:2439486/93‑1 (MQ=255)
                      aGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTttatta                                                                   >  1:3325270/1‑93 (MQ=255)
                      aGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTttatta                                                                   <  1:4011205/93‑1 (MQ=255)
                       ggCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTAc                                                                  >  2:2745475/1‑93 (MQ=255)
                        gCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTAcc                                                                 >  1:4205853/1‑93 (MQ=255)
                         cTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACct                                                                <  2:221840/93‑1 (MQ=255)
                         cTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACct                                                                >  1:4638534/1‑93 (MQ=255)
                            cGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTa                                                             <  2:3394408/93‑1 (MQ=255)
                             ggTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTAc                                                            <  1:1073061/93‑1 (MQ=255)
                             ggTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTAc                                                            >  2:1198633/1‑93 (MQ=255)
                             ggTAAGTCTGCGGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTAc                                                            <  1:1415817/93‑1 (MQ=255)
                              gTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACg                                                           >  1:1301018/1‑93 (MQ=255)
                               tAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGc                                                          <  1:598389/93‑1 (MQ=255)
                                  gTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGc                                                       >  1:5945909/1‑93 (MQ=255)
                                  gTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTGATTACCTCTACGCTGc                                                       >  2:2320808/1‑93 (MQ=255)
                                     tgctgGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGcaac                                                    >  1:5872491/1‑93 (MQ=255)
                                      tctgGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGcaaca                                                   >  1:1208177/2‑93 (MQ=255)
                                       ctgGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGcaacaa                                                  <  2:573479/93‑1 (MQ=255)
                                        tgGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAg                                                 >  1:5370400/1‑93 (MQ=255)
                                        tgGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAg                                                 <  1:1863484/93‑1 (MQ=255)
                                           aaaaaGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTg                                              >  2:4915910/1‑93 (MQ=255)
                                           aaaaaGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGATGCAACAAGCTg                                              >  2:5944501/1‑93 (MQ=255)
                                            aaaaGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGc                                             >  1:6022827/1‑93 (MQ=255)
                                            aaaaGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGc                                             <  1:4524159/93‑1 (MQ=255)
                                               aGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACACGCTGCCAg                                          >  1:4962752/1‑93 (MQ=255)
                                                gcgcgcTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGc                                         >  2:1936828/1‑93 (MQ=255)
                                                 cgcgcTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCCGCa                                        >  2:3167532/1‑93 (MQ=255)
                                                 cgcgcTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCa                                        <  2:1495081/93‑1 (MQ=255)
                                                  gcgcTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCAc                                       <  1:5834856/93‑1 (MQ=255)
                                                  gcgcTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCAc                                       >  2:2967585/1‑93 (MQ=255)
                                                   cgcTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCAcc                                      <  2:6117559/93‑1 (MQ=255)
                                                   cgcTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCAcc                                      <  2:3950203/93‑1 (MQ=255)
                                                     cTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCg                                    <  1:6357001/93‑1 (MQ=255)
                                                       aCAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTc                                  >  2:4584251/1‑93 (MQ=255)
                                                       aCAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTc                                  <  1:5130983/93‑1 (MQ=255)
                                                       aCAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTc                                  >  1:5258733/1‑93 (MQ=255)
                                                       aCAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTc                                  <  2:545895/93‑1 (MQ=255)
                                                       aCAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTc                                  >  1:2912836/1‑93 (MQ=255)
                                                       aCAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTc                                  >  2:5653983/1‑93 (MQ=255)
                                                         aGCGTGCTGGAACGTGAAGACAACCCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCtt                                <  1:226598/93‑1 (MQ=255)
                                                         aGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCtt                                <  2:3005082/93‑1 (MQ=255)
                                                         aGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCtt                                <  2:4856352/93‑1 (MQ=255)
                                                          gCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTg                               <  2:1733740/93‑1 (MQ=255)
                                                          gCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTg                               >  1:1768560/1‑93 (MQ=255)
                                                          gCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTg                               >  1:2015483/1‑93 (MQ=255)
                                                          gCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTg                               <  2:835521/93‑1 (MQ=255)
                                                          gCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTg                               >  2:2718063/1‑93 (MQ=255)
                                                           cGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTgg                              <  1:2707016/93‑1 (MQ=255)
                                                            gTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGa                             >  2:3626540/1‑93 (MQ=255)
                                                            gTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGa                             >  2:1144325/1‑93 (MQ=255)
                                                             tGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGAt                            <  2:1628131/93‑1 (MQ=255)
                                                             tGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGAt                            >  1:1508825/1‑93 (MQ=255)
                                                             tGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACACGTCTTGGAt                            >  1:2325727/1‑93 (MQ=255)
                                                              gCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGAtt                           >  1:2994812/1‑93 (MQ=255)
                                                                 ggAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTgg                        >  2:6066680/1‑93 (MQ=255)
                                                                    aCGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGTCGt                     <  1:3752575/93‑1 (MQ=255)
                                                                    aCGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAACCTGCCAGCACCCGTCTTGGATTTGGCGt                     >  2:3227554/1‑93 (MQ=255)
                                                                     cGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGCGTg                    >  1:1039893/1‑93 (MQ=255)
                                                                       tGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCACCAAGCTGTCAGCACCCGTCTTGGATTTGGCGTGaa                  >  1:957398/1‑93 (MQ=255)
                                                                       tGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGCGTGaa                  <  1:746391/93‑1 (MQ=255)
                                                                           gACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGCGTGaaaaaa              <  1:3188943/93‑1 (MQ=255)
                                                                            aCAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGCGTGaaaaaaa             <  1:4928050/93‑1 (MQ=255)
                                                                              aaaCCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGCGTGAAAAAAAcc           <  2:431557/93‑1 (MQ=255)
                                                                               aaCCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGCGTGAAAAAAACCa          <  1:6123919/93‑1 (MQ=255)
                                                                                aCCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGCGTGAAAAAAACCat         >  2:511360/1‑93 (MQ=255)
                                                                                  cGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGCGTGAAAAAAACCatga       >  2:484645/1‑93 (MQ=255)
                                                                                   gACAACCGGTAAACCTGGCGCTCCTTGTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGCGTGAAAAAAACCatgat      <  2:4468436/93‑1 (MQ=255)
                                                                                    aCAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGCGTGAAAAAAACCatgatg     >  2:4669030/1‑93 (MQ=255)
                                                                                    aCAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGCGTGAAAAAAACCatgatg     <  1:642746/93‑1 (MQ=255)
                                                                                     cAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGCGTGAAAAAGACCatgatga    >  1:3103074/1‑93 (MQ=255)
                                                                                     cAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGCGTGAAAAAAACCatgatga    >  1:2942561/1‑93 (MQ=255)
                                                                                     cAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGCGTGAAAAAAACCatgatga    >  1:2564548/1‑93 (MQ=255)
                                                                                      aaCCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGCGTGAAAAAAACCatgatgat   <  2:4069255/93‑1 (MQ=255)
                                                                                       aCCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGCGTGAAAAAAACCatgatgatg  >  2:953889/1‑93 (MQ=255)
                                                                                          |                                                                                         
CCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGCGTGAAAAAAACCATGATGATG  >  NC_000913/1329813‑1329992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑