BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,457,617	T→C	P180P (CCT→CCC)	ydbU →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,457,617	0	T	C	99.6%	773.8 / NA	223	P180P (CCT→CCC)	ydbU	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (101/121); minor base A (1/0); total (102/121)
Fisher's exact test for biased strand distribution p-value = 4.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 230 total aligned reads displayed.

GAAGTTGGTGGAGGTGGTCAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCTGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCTGGCGGGCACTGGAAGAGCAGGT  >  NC_000913/1457527‑1457706

gaAGTTGGTGGAGGTGGTCAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCcgt                                                                                         <  2:3626533/93‑1 (MQ=255)

  aGTTGGTGGAGGTGGTCAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTgc                                                                                       <  1:2112391/93‑1 (MQ=255)

   gTTGGTGGAGGTGGTCAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTgcg                                                                                      <  1:4916258/93‑1 (MQ=255)

    ttGGTGGAGGTGGTCAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTgcgc                                                                                     <  1:4492670/93‑1 (MQ=255)

      ggtggAGGTGGTCAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTg                                                                                   <  1:4065119/93‑1 (MQ=255)

        tggAGGTGGTCAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTc                                                                                 >  2:6341746/1‑93 (MQ=255)

           aGGTGGTCAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCTGTGCGCTGTCAtt                                                                              >  1:1499127/1‑93 (MQ=255)

           aGGTGGTCAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCAtt                                                                              <  2:5340731/93‑1 (MQ=255)

            ggtggtCAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTc                                                                             >  2:5480540/1‑93 (MQ=255)

             gtggtCAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCg                                                                            >  2:2646168/1‑93 (MQ=255)

               ggtCAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGAc                                                                          >  1:5329547/1‑93 (MQ=255)

                  cAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTcc                                                                       >  1:3526378/1‑93 (MQ=255)

                  cAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTcc                                                                       <  2:4926196/93‑1 (MQ=255)

                   aGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCt                                                                      <  1:324786/93‑1 (MQ=255)

                   aGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCt                                                                      >  2:3651426/1‑93 (MQ=255)

                   aGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCt                                                                      <  2:4070739/93‑1 (MQ=255)

                    gTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAGCGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTg                                                                     >  1:6029035/1‑93 (MQ=255)

                    gTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTg                                                                     >  2:1671823/1‑93 (MQ=255)

                    gTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTg                                                                     >  2:4859773/1‑93 (MQ=255)

                     tGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTgg                                                                    <  2:2507847/93‑1 (MQ=255)

                     tGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTgg                                                                    <  2:82081/93‑1 (MQ=255)

                        gCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCATCCCGTGCGCTGTCATTCGACTCCTGGAtt                                                                 <  2:6383557/93‑1 (MQ=255)

                         cTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGAttt                                                                <  2:876782/93‑1 (MQ=255)

                         cTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGAttt                                                                <  1:2712132/93‑1 (MQ=255)

                          tGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTa                                                               >  2:1521588/1‑93 (MQ=255)

                           ggCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCAGTGCGCTGTCATTCGACTCCTGGCTTTAt                                                              >  2:5811734/1‑93 (MQ=255)

                            gCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATc                                                             <  2:3854392/93‑1 (MQ=255)

                            gCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATc                                                             <  2:5855792/93‑1 (MQ=255)

                             cAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCg                                                            <  1:2893175/93‑1 (MQ=255)

                               aCGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGtt                                                          <  2:2247586/93‑1 (MQ=255)

                               aCGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGtt                                                          >  2:5707630/1‑93 (MQ=255)

                                cggcggcggAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTa                                                         >  2:1378192/1‑93 (MQ=255)

                                 ggcggcggAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTaa                                                        >  2:5240437/1‑93 (MQ=255)

                                 ggcggcggAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTaa                                                        >  1:2468324/1‑93 (MQ=255)

                                  gcggcggAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAAc                                                       >  1:5810153/1‑93 (MQ=255)

                                  gcggcggAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAAc                                                       <  1:1412755/93‑1 (MQ=255)

                                  gcggcggAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAAc                                                       >  1:487828/1‑93 (MQ=255)

                                  gcggcggAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAAc                                                       <  2:1991281/93‑1 (MQ=255)

                                       ggAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCgtgt                                                  <  1:6330629/93‑1 (MQ=255)

                                       ggAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCgtgt                                                  >  1:1043651/1‑93 (MQ=255)

                                       ggAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCgtgt                                                  <  2:5152884/93‑1 (MQ=255)

                                        gAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCATCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGtt                                                 <  2:1702894/93‑1 (MQ=255)

                                        gAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGtt                                                 >  2:5728327/1‑93 (MQ=255)

                                         aaGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCATCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTg                                                <  2:6174185/93‑1 (MQ=255)

                                          aGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTgc                                               >  2:4588590/1‑93 (MQ=255)

                                          aGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTgc                                               <  2:648662/93‑1 (MQ=255)

                                          aGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTgc                                               >  2:5012498/1‑93 (MQ=255)

                                            ttgttgAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTgcgc                                             <  2:2051009/93‑1 (MQ=255)

                                              gttgAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGt                                           >  2:1482370/1‑93 (MQ=255)

                                               ttgAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGCTTTAt                                                              >  1:389797/1‑73 (MQ=255)

                                               ttgAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTAt                                                              <  2:389797/73‑1 (MQ=255)

                                               ttgAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGATGCGCGTc                                          >  1:3946370/1‑93 (MQ=255)

                                                tgAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGGCTCCTGGATTTATCGTTAACCGTGTTGCGCGTcc                                         <  1:1307747/93‑1 (MQ=255)

                                                tgAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTcc                                         <  1:396231/93‑1 (MQ=255)

                                                tgAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTcc                                         <  1:3674410/93‑1 (MQ=255)

                                                 gAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCt                                        >  1:5077636/1‑93 (MQ=255)

                                                 gAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCt                                        <  2:1308195/93‑1 (MQ=255)

                                                 gAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCt                                        >  2:3301050/1‑93 (MQ=255)

                                                 gAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCt                                        >  1:362135/1‑93 (MQ=255)

                                                  aGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCtt                                       <  1:4125419/93‑1 (MQ=255)

                                                   gCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCtta                                      <  2:1741828/93‑1 (MQ=255)

                                                     aGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCttatt                                    >  1:2375877/1‑93 (MQ=255)

                                                       ttGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTtattat                                  <  2:4448236/93‑1 (MQ=255)

                                                        tgtgtgAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTattatt                                 <  1:2596741/93‑1 (MQ=255)

                                                        tgtgtgAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTattatt                                 >  1:4405515/1‑93 (MQ=255)

                                                         gtgtgAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTc                                <  2:4575832/93‑1 (MQ=255)

                                                           gtgAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCg                              >  2:354417/1‑93 (MQ=255)

                                                            tgAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGa                             <  1:5461931/93‑1 (MQ=255)

                                                            tgAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGa                             <  1:1020560/93‑1 (MQ=255)

                                                             gAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAg                            >  2:4270585/1‑93 (MQ=255)

                                                             gAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAg                            >  1:3130860/1‑93 (MQ=255)

                                                              aaCTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAgg                           >  1:931268/1‑93 (MQ=255)

                                                                cTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGcc                         <  2:3133621/93‑1 (MQ=255)

                                                                cTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGcc                         <  2:2796256/93‑1 (MQ=255)

                                                                cTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGcc                         >  1:2503142/1‑93 (MQ=255)

                                                                 tAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCt                        <  1:2919906/93‑1 (MQ=255)

                                                                 tAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCt                        >  1:5637801/1‑93 (MQ=255)

                                                                  aaCGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCTg                       <  2:958261/93‑1 (MQ=255)

                                                                  aaCGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCAAGGCCTg                       >  1:557228/1‑93 (MQ=255)

                                                                   aCGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCTgg                      <  2:4052018/93‑1 (MQ=255)

                                                                   aCGTTGAGGTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCTgg                      >  1:6313088/1‑93 (MQ=255)

                                                                     gTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCTGGCg                    <  1:5775651/93‑1 (MQ=255)

                                                                     gTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCTGGCg                    >  1:1373271/1‑93 (MQ=255)

                                                                      ttGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCTGGCgg                   <  1:2231587/93‑1 (MQ=255)

                                                                       tGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCTGGCggg                  <  2:2418329/93‑1 (MQ=255)

                                                                       tGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCTGGCggg                  <  1:6232978/93‑1 (MQ=255)

                                                                        gAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCTGGCGGGc                 >  2:2863468/1‑93 (MQ=255)

                                                                           ttGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCTGGCGGGCACt              <  2:4743865/93‑1 (MQ=255)

                                                                            tGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCTGGCGGGCACTg             <  1:1138196/93‑1 (MQ=255)

                                                                                gTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCTGGCGGGCACTGGAag         >  1:1899947/1‑93 (MQ=255)

                                                                                  aaGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCTGGCGGGCACTGGAagag       <  1:3640467/93‑1 (MQ=255)

                                                                                  aaGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCTGGCGGGCACTGGAagag       >  2:2970135/1‑93 (MQ=255)

                                                                                   agcagcCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCTGGCGGGCACTGGAAGAGc      <  2:1187918/93‑1 (MQ=255)

                                                                                   agcagcCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCTGGCGGGCACTGGAAGAGc      >  2:6111793/1‑93 (MQ=255)

                                                                                   agcagcCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCTGGCGGGCACTGGAAGAGc      <  1:4594926/93‑1 (MQ=255)

                                                                                    gcagcCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCTGGCGGGCACTGGAAGAGCa     <  1:4613554/93‑1 (MQ=255)

                                                                                      agcCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCTGGCGGGCACTGGAAGAGCAgg   >  2:3388737/1‑93 (MQ=255)

                                                                                      agcCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCTGGCGGGCACTGGAAGAGCAgg   >  1:4234649/1‑93 (MQ=255)

                                                                                       gcCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCTGGCGGGCACTGGAAGAGCAGGt  >  2:1400050/1‑93 (MQ=255)

                                                                                       gcCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCTGGCGGGCACTGGAAGAGCAGGt  >  1:31096/1‑93 (MQ=255)

GAAGTTGGTGGAGGTGGTCAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCTGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCTGGCGGGCACTGGAAGAGCAGGT  >  NC_000913/1457527‑1457706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`