BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,482,256	C→T	A391V (GCG→GTG)	hrpA →	helicase, ATP‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,482,256	0	C	T	99.2%	884.0 / ‑5.7	241	A391V (GCG→GTG)	hrpA	helicase, ATP‑dependent
Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (109/128); total (112/129)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 247 total aligned reads displayed.

AATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCC  >  NC_000913/1482166‑1482345

gaTACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGgtgt                                                                                         >  2:6157661/2‑93 (MQ=255)

aaTACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGgtgt                                                                                         >  2:2803819/1‑93 (MQ=255)

 aTACGTTATCGACCCGAGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTc                                                                                        <  1:5738301/93‑1 (MQ=255)

 aTACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTc                                                                                        <  2:125813/93‑1 (MQ=255)

 aTACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTc                                                                                        >  1:2243901/1‑93 (MQ=255)

  tACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCt                                                                                       >  1:3894953/1‑93 (MQ=255)

  tACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCt                                                                                       >  1:2948316/1‑93 (MQ=255)

    cGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACATCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGc                                                                                     >  1:1909072/1‑93 (MQ=255)

      ttATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCa                                                                                   <  2:795436/93‑1 (MQ=255)

       tATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCGGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCaa                                                                                  <  2:4646470/93‑1 (MQ=255)

        aTCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAAt                                                                                 >  1:1145831/1‑93 (MQ=255)

            aCCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGc                                                                             >  1:5010781/1‑93 (MQ=255)

            aCCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGc                                                                             <  2:5924836/93‑1 (MQ=255)

            aCCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGc                                                                             <  2:4774012/93‑1 (MQ=255)

               ccGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTa                                                                          >  1:1697203/1‑93 (MQ=255)

               ccGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTa                                                                          >  2:3144120/1‑93 (MQ=255)

                cGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTaa                                                                         >  2:3862358/1‑93 (MQ=255)

                 ggTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTaaa                                                                        >  2:226347/1‑93 (MQ=255)

                 ggTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTaaa                                                                        >  2:5757664/1‑93 (MQ=255)

                 ggTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTaaa                                                                        >  1:4441357/1‑93 (MQ=255)

                   tACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAgg                                                                      <  2:5155779/93‑1 (MQ=255)

                    aCAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGc                                                                     <  2:5824587/93‑1 (MQ=255)

                     cAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGcc                                                                    <  1:2537341/93‑1 (MQ=255)

                      aGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCg                                                                   >  2:1382301/1‑93 (MQ=255)

                       gcgcGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGc                                                                  <  1:4146120/93‑1 (MQ=255)

                       gcgcGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGc                                                                  >  2:2519670/1‑93 (MQ=255)

                       gcgcGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGc                                                                  >  2:5244471/1‑93 (MQ=255)

                         gcgTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCtg                                                                <  1:1398257/93‑1 (MQ=255)

                         gcgTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCtg                                                                <  1:313816/93‑1 (MQ=255)

                          cgTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCtgt                                                               <  1:603263/93‑1 (MQ=255)

                           gTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCtgtg                                                              >  1:40632/1‑93 (MQ=255)

                           gTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTATCTGCCAATCAGCGTAAAGGCCGCtgtg                                                              >  1:3190207/1‑93 (MQ=255)

                             aTCAGCCGCTACAGCTATCTGACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCTCAGGCGACTGCCAATCAGCGTAAAGGCAGCTGTggc                                                            >  1:5944099/1‑92 (MQ=255)

                               cAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCg                                                          >  2:1887972/1‑93 (MQ=255)

                                aGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCgt                                                         >  2:4402358/1‑93 (MQ=255)

                                 gCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCgtg                                                        >  2:2880131/1‑93 (MQ=255)

                                 gCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCgtg                                                        >  1:2793630/1‑93 (MQ=255)

                                  ccGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCgtgt                                                       <  1:5137700/93‑1 (MQ=255)

                                  ccGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCgtgt                                                       <  1:3794478/93‑1 (MQ=255)

                                   cGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCgtgtg                                                      <  2:1959114/93‑1 (MQ=255)

                                   cGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCgtgtg                                                      <  1:5222147/93‑1 (MQ=255)

                                    gCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCgtgtgt                                                     >  1:3952511/1‑93 (MQ=255)

                                     cTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTc                                                    <  1:1196500/93‑1 (MQ=255)

                                     cTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTc                                                    >  1:1004224/1‑93 (MQ=255)

                                      tACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTcc                                                   >  2:2044776/1‑93 (MQ=255)

                                      tACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTcc                                                   <  2:5017356/93‑1 (MQ=255)

                                         aGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGaa                                                <  1:1563781/93‑1 (MQ=255)

                                          gCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAg                                               <  1:6476096/93‑1 (MQ=255)

                                          gCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAg                                               <  2:4069512/93‑1 (MQ=255)

                                            tATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGGAggg                                             >  1:2904834/1‑93 (MQ=255)

                                              tCGCCCCAAAGTTCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGAt                                           <  1:4719152/93‑1 (MQ=255)

                                              tCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGAt                                           <  1:5193590/93‑1 (MQ=255)

                                              tCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGAt                                           <  1:2892395/93‑1 (MQ=255)

                                               cGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATc                                          <  1:3262502/93‑1 (MQ=255)

                                                gCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCt                                         <  1:4270079/93‑1 (MQ=255)

                                                gCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCt                                         >  1:2139352/1‑93 (MQ=255)

                                                gCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCt                                         >  1:3374548/1‑93 (MQ=255)

                                                  aCCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGAGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGt                                       >  1:364295/1‑93 (MQ=255)

                                                   ccAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTa                                      <  1:4386078/93‑1 (MQ=255)

                                                   ccAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTa                                      <  2:4196374/93‑1 (MQ=255)

                                                   ccAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTa                                      <  1:3054952/93‑1 (MQ=255)

                                                     aaaGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTAtt                                    >  2:6029516/1‑93 (MQ=255)

                                                        gTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGt                                 >  2:4670692/1‑93 (MQ=255)

                                                        gTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGt                                 >  1:2162780/1‑93 (MQ=255)

                                                        gTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGt                                 <  2:3282647/93‑1 (MQ=255)

                                                         tGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGtc                                <  1:6186250/93‑1 (MQ=255)

                                                         tGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGtc                                <  2:5539801/93‑1 (MQ=255)

                                                          gCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGtct                               >  2:3194903/1‑93 (MQ=255)

                                                          gCAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGtct                               <  2:3672889/93‑1 (MQ=255)

                                                           cAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGtctc                              <  2:64329/93‑1 (MQ=255)

                                                           cAGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGtctc                              <  2:3408470/93‑1 (MQ=255)

                                                            aGCGTTTGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTctct                             <  1:89658/93‑1 (MQ=255)

                                                            aGCGTTTACCGATTGAGCCGATCTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTctct                             <  2:5362515/93‑1 (MQ=255)

                                                                tttGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTc                         <  1:4460476/93‑1 (MQ=255)

                                                                tttGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTc                         >  1:2849601/1‑93 (MQ=255)

                                                                tttGCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTc                         >  2:4096518/1‑93 (MQ=255)

                                                                   gacGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGa                      >  1:4056323/3‑93 (MQ=255)

                                                                   gCCGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGa                      >  1:5541350/1‑93 (MQ=255)

                                                                    ccGATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGaa                     <  1:1485913/93‑1 (MQ=255)

                                                                      gATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGa                   >  1:1890592/1‑93 (MQ=255)

                                                                      gATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGa                   >  2:4006148/1‑93 (MQ=255)

                                                                      gATTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGa                   <  2:1056715/93‑1 (MQ=255)

                                                                       aTTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGAc                  >  1:6305930/1‑93 (MQ=255)

                                                                       aTTGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGAc                  >  1:4565331/1‑93 (MQ=255)

                                                                       aTTGAGCCGATTTCCCAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGAc                  <  2:2470468/93‑1 (MQ=255)

                                                                        ttGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACg                 >  2:355589/1‑93 (MQ=255)

                                                                         tGAGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGa                >  2:2776821/1‑93 (MQ=255)

                                                                           aGCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGAtt              <  2:1627600/93‑1 (MQ=255)

                                                                            gCCGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGAttt             <  2:72020/93‑1 (MQ=255)

                                                                             ccGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTc            <  1:5899966/93‑1 (MQ=255)

                                                                              cGATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTcc           >  1:6238339/1‑93 (MQ=255)

                                                                               gATTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCct          <  1:792990/93‑1 (MQ=255)

                                                                                aTTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCctc         <  1:4666316/93‑1 (MQ=255)

                                                                                aTTTCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCctc         <  2:193144/93‑1 (MQ=255)

                                                                                 tttCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCctct        <  2:4468206/93‑1 (MQ=255)

                                                                                  ttCCCAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCtctc       <  1:4521627/93‑1 (MQ=255)

                                                                                    cccAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTcgc     >  1:666352/1‑93 (MQ=255)

                                                                                    cccAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTcgc     <  1:5385461/93‑1 (MQ=255)

                                                                                      cAGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCgcgc   >  1:309317/1‑93 (MQ=255)

                                                                                       aGGTGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGcc  >  2:6351675/1‑93 (MQ=255)

AATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCC  >  NC_000913/1482166‑1482345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`