Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,603,543 T→C F157L (TTT→CTT)  b1516 → putative LACI‑type transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,603,5430TC99.4% 599.5 / NA 176F157L (TTT→CTT) b1516putative LACI‑type transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (91/84);  total (91/85)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 187 total aligned reads displayed.

TACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGTTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTGAAAGAAGCGAAAGCGAAAATCGCCAAAGAGCATCCAGGCTGGGAA  >  NC_000913/1603453‑1603632
                                                                                          |                                                                                         
tacATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGctt                                                                                         <  2:2312957/93‑1 (MQ=255)
 acATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGcttt                                                                                        <  1:4695314/93‑1 (MQ=255)
  cATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGctttt                                                                                       <  2:6384967/93‑1 (MQ=255)
  cATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGctttt                                                                                       >  2:6329704/1‑93 (MQ=255)
  cATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGctttt                                                                                       <  2:4786634/93‑1 (MQ=255)
   aTTAATCAGGGAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTc                                                                                      <  2:1663409/93‑1 (MQ=255)
    ttAATCAGGGAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCt                                                                                     <  2:5622001/93‑1 (MQ=255)
    ttAATCAGGGAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCt                                                                                     <  1:2472167/93‑1 (MQ=255)
         cAGGGAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCa                                                                                >  2:1598137/1‑93 (MQ=255)
          aGGGAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCaa                                                                               >  2:2357972/1‑93 (MQ=255)
          aGGGAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCaa                                                                               >  2:1085484/1‑93 (MQ=255)
           gggAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAg                                                                              >  2:6354925/1‑93 (MQ=255)
             gAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGcc                                                                            <  1:2642144/93‑1 (MQ=255)
                cgcccgccCAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCa                                                                         <  2:5597471/93‑1 (MQ=255)
                cgcccgccCAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCa                                                                         >  1:3170835/1‑93 (MQ=255)
                  cccgcccAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCAcc                                                                       >  2:3708546/1‑93 (MQ=255)
                   ccgcccAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCg                                                                      >  2:1865812/1‑93 (MQ=255)
                   ccgcccAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCg                                                                      >  1:528889/1‑93 (MQ=255)
                   ccgcccAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCg                                                                      >  1:2720214/1‑93 (MQ=255)
                   ccgcccAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCg                                                                      >  2:5776967/1‑93 (MQ=255)
                    cgcccAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGt                                                                     <  1:583616/93‑1 (MQ=255)
                    cgcccAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGt                                                                     >  1:2806579/1‑93 (MQ=255)
                    cgcccAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGt                                                                     <  1:4408/93‑1 (MQ=255)
                      cccAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTa                                                                   >  2:5387636/1‑93 (MQ=255)
                      cccAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTa                                                                   >  1:6082312/1‑93 (MQ=255)
                       ccAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTAc                                                                  <  2:4605047/93‑1 (MQ=255)
                       ccAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTAc                                                                  >  1:2610929/1‑93 (MQ=255)
                        cAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACg                                                                 >  2:2965022/1‑93 (MQ=255)
                        cAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACg                                                                 <  2:385316/93‑1 (MQ=255)
                         aGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACgg                                                                >  2:2773890/1‑93 (MQ=255)
                         aGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACgg                                                                >  2:5942295/1‑93 (MQ=255)
                         aGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACgg                                                                >  2:827897/1‑93 (MQ=255)
                          attAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGa                                                               <  2:2174734/92‑1 (MQ=255)
                           ttAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGAc                                                              <  1:4201248/93‑1 (MQ=255)
                           ttAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGAc                                                              <  2:5763531/93‑1 (MQ=255)
                            tAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGAcc                                                             >  1:6363332/1‑93 (MQ=255)
                            tAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGAcc                                                             <  2:4241196/93‑1 (MQ=255)
                               gaggTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCaaa                                                          <  2:4017263/93‑1 (MQ=255)
                               gaggTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCaaa                                                          >  1:1058832/1‑93 (MQ=255)
                                aggTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCaaaa                                                         <  2:4969585/93‑1 (MQ=255)
                                   tATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCa                                                      <  1:717690/93‑1 (MQ=255)
                                    aTGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAg                                                     <  1:5354043/93‑1 (MQ=255)
                                     tGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGt                                                    >  2:2602356/1‑93 (MQ=255)
                                      tttGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGTTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTg                                                   <  1:1177960/92‑1 (MQ=255)
                                      gTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTg                                                   <  2:107223/93‑1 (MQ=255)
                                      gTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTg                                                   <  1:3653151/93‑1 (MQ=255)
                                       ttGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTgg                                                  <  1:1309350/93‑1 (MQ=255)
                                       ttGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTgg                                                  <  2:1436753/93‑1 (MQ=255)
                                        tggtggATATGGCGGCGCTTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTTCTCAAGCCCCACCGTTACGGACCAAAACCAGTggg                                                 <  1:100393/93‑1 (MQ=255)
                                         ggtggATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGt                                                <  2:1558514/93‑1 (MQ=255)
                                          gtggATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTg                                               <  2:1236644/93‑1 (MQ=255)
                                           tggATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTGa                                              >  1:3598734/1‑93 (MQ=255)
                                            ggATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTGaa                                             >  2:1649209/1‑93 (MQ=255)
                                            ggATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTGaa                                             <  2:1890696/93‑1 (MQ=255)
                                             gATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTGaaa                                            >  2:4880671/1‑93 (MQ=255)
                                             gATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTGaaa                                            <  1:5866471/93‑1 (MQ=255)
                                             gATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTGaaa                                            <  1:3022243/93‑1 (MQ=255)
                                                atGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTGAaagaa                                         <  1:4883781/93‑1 (MQ=255)
                                                 tGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTGAaagaag                                        >  1:1246917/1‑93 (MQ=255)
                                                  ggcggcGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTGAAAGaagc                                       <  1:659901/93‑1 (MQ=255)
                                                  ggcggcGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTGAAAGaagc                                       <  2:1168184/93‑1 (MQ=255)
                                                   gcggcgCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTGAAAGaagcg                                      >  2:554553/1‑93 (MQ=255)
                                                    cggcgCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTGAAAGaagcga                                     >  1:512423/1‑93 (MQ=255)
                                                      gcgcGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTGAAAGaagcgaaa                                   <  1:6229252/93‑1 (MQ=255)
                                                        gcgTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTGAAAGaagcgaaagc                                 <  2:2427587/93‑1 (MQ=255)
                                                         cgTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTGAAAGaagcgaaagcg                                <  1:5566694/93‑1 (MQ=255)
                                                         cgTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTGAAAGaagcgaaagcg                                <  2:4764302/93‑1 (MQ=255)
                                                         cgTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTGAAAGaagcgaaagcg                                >  1:3435798/1‑93 (MQ=255)
                                                          gTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTGAAAGaagcgaaagcga                               >  2:5495018/1‑93 (MQ=255)
                                                          gTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTGAAAGaagcgaaagcga                               <  2:3797220/93‑1 (MQ=255)
                                                          gTCAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTGAAAGaagcgaaagcga                               >  2:3849760/1‑93 (MQ=255)
                                                            cAGGTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTGAAAGAAgcgaaagcgaaa                             >  1:2871201/1‑93 (MQ=255)
                                                              ggTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTGAAAGAAGCGAAAGCGAAAAt                           <  1:1866989/93‑1 (MQ=255)
                                                              ggTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTGAAAGAAGCGAAAGCGAAAAt                           >  1:795934/1‑93 (MQ=255)
                                                              ggTGAATAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTGAAAGAAGCGAAAGCGAAAAt                           <  2:2352896/93‑1 (MQ=255)
                                                                  aaTAAAGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTGAAAGAAGCGAAAGCGAAAATCGcc                       <  1:4768787/93‑1 (MQ=255)
                                                                     aaaGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTGAAAGAAGCGAAAGCGAAAATCGCCaaa                    >  1:4411364/1‑93 (MQ=255)
                                                                      aaGACAAAGCCAAAGTGGCGCTTTTCTGCTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTGAAAGAAGCGAAAGCGAAAATCGCCAAag                   <  1:2105411/93‑1 (MQ=255)
                                                                      aaGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTGAAAGAAGCGAAAGCGAAAATCGCCAAag                   >  1:869982/1‑93 (MQ=255)
                                                                      aaGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTGAAAGAAGCGAAAGCGAAAATCGCCAAag                   <  1:6376459/93‑1 (MQ=255)
                                                                      aaGACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGAGAAAGAAGCGAAAGCGAAAATCGCCAAag                   >  2:2026233/1‑93 (MQ=255)
                                                                        gACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTGAAAGAAGCGAAAGCGAAAATCGCCAAagag                 >  1:382610/1‑93 (MQ=255)
                                                                        gACAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTGAAAGAAGCGAAAGCGAAAATCGCCAAagag                 >  2:2988731/1‑93 (MQ=255)
                                                                         aCAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTGAAAGAAGCGAAAGCGAAAATCGCCAAAGAGc                >  2:2380520/1‑93 (MQ=255)
                                                                         aCAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTGAAAGAAGCGAAAGCGAAAATCGCCAAAGAGc                >  2:1670344/1‑93 (MQ=255)
                                                                          cAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTGAAAGAAGCGAAAGCGAAAATCGCCAAAGAGCa               <  2:5270547/93‑1 (MQ=255)
                                                                          cAAAGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTGAAAGAAGCGAAAGCGAAAATCGCCAAAGAGCa               <  2:3205656/93‑1 (MQ=255)
                                                                           aaaGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTGAAAGAAGCGAAAGCGAAAATCGCCAAAGAGCAt              <  2:2495421/93‑1 (MQ=255)
                                                                            aaGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTGAAAGAAGCGAAAGCGAAAATCGCCAAAGAGCATc             >  2:3325100/1‑93 (MQ=255)
                                                                             aGCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTGAAAGAAGCGAAAGCGAAAATCGCCAAAGAGCATcc            <  1:3272826/93‑1 (MQ=255)
                                                                              gCCAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTGAAAGAAGCGAAAGCGAAAATCGCCAAAGAGCATCCa           >  1:2307596/1‑93 (MQ=255)
                                                                               ccAAAGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTGAAAGAAGCGAAAGCGAAAATCGCCAAAGAGCATCCAg          >  1:5436760/1‑93 (MQ=255)
                                                                               ccAAAGTCGCGCATTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTGAAAGAAGCGAAAGCGAAAATCGCCAAAGAGCATCCAg          >  2:3466409/1‑93 (MQ=255)
                                                                                 aaaGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTGAAAGAAGCGAAAGCGAAAATCGCCAAAGAGCATCCAGGc        <  2:1279601/93‑1 (MQ=255)
                                                                                 aaaGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTGAAAGAAGCGAAAGCGAAAATCGCCAAAGAGCATCCAGGc        >  2:740563/1‑93 (MQ=255)
                                                                                 aaaGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTGAAAGAAGCGAAAGCGAAAATCGCCAAAGAGCATCCAGGc        <  2:1000714/93‑1 (MQ=255)
                                                                                 aaaGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTGAAAGAAGCGAAAGCGAAAATCGCCAAAGAGCATCCAGGc        >  2:2694994/1‑93 (MQ=255)
                                                                                  aaGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTGAAAGAAGCGAAAGCGAAAATCGCCAAAGAGCATCCAGGCt       >  2:3944390/1‑93 (MQ=255)
                                                                                  aaGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTGAAAGAAGCGAAAGCGAAAATCGCCAAAGAGCATCCAGGCt       >  1:3995294/1‑93 (MQ=255)
                                                                                   aGTCGCGCTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTGAAAGAAGCGAAAGCGAAAATCGCCAAAGAGCATCCAGGCTg      <  1:545415/93‑1 (MQ=255)
                                                                                          |                                                                                         
TACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGTTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTGAAAGAAGCGAAAGCGAAAATCGCCAAAGAGCATCCAGGCTGGGAA  >  NC_000913/1603453‑1603632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑