Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,652,626 T→C E180E (GAA→GAG)  rspA ← starvation sensing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,652,6260TC99.6% 791.6 / ‑4.8 226E180E (GAA→GAG) rspAstarvation sensing protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (117/108);  minor base G (1/0);  total (118/108)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 247 total aligned reads displayed.

CAGCAAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGTTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCC  >  NC_000913/1652536‑1652715
                                                                                          |                                                                                         
cagcaAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGctc                                                                                         <  1:5971409/93‑1 (MQ=255)
 agcaAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGctct                                                                                        >  1:1987428/1‑93 (MQ=255)
 agcaAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGctct                                                                                        <  2:6018109/93‑1 (MQ=255)
  gcaAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGctctt                                                                                       >  1:2165576/1‑93 (MQ=255)
  gcaAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGctctt                                                                                       <  2:2996811/93‑1 (MQ=255)
   caAATGTTCATTAAAACCAAGCTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGctcttc                                                                                      <  2:3969183/93‑1 (MQ=255)
   caAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGctcttc                                                                                      <  1:1645059/93‑1 (MQ=255)
   caAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGctcttc                                                                                      <  1:1670574/93‑1 (MQ=255)
   caAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGctcttc                                                                                      <  2:27443/93‑1 (MQ=255)
     aaTGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCg                                                                                    >  1:3692342/1‑93 (MQ=255)
       tGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGc                                                                                  <  2:489404/93‑1 (MQ=255)
          tCATTAAAACAAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCCTCGACCACAGCTGCTCTTCCGGCCAc                                                                               >  1:1880632/1‑93 (MQ=255)
           taTTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACt                                                                              <  2:4507754/92‑1 (MQ=255)
           cATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACt                                                                              <  2:3552082/93‑1 (MQ=255)
            aTTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTg                                                                             >  1:3203700/1‑93 (MQ=255)
             ttAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGt                                                                            >  1:4753829/1‑93 (MQ=255)
                 aaCCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCtt                                                                        >  1:3277963/1‑93 (MQ=255)
                 aaCCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCtt                                                                        >  2:3857917/1‑93 (MQ=255)
                 aaCCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCtt                                                                        <  2:3581843/93‑1 (MQ=255)
                 aaCCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTATCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCtt                                                                        <  2:210778/93‑1 (MQ=255)
                  aCCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCttt                                                                       <  2:1851745/93‑1 (MQ=255)
                  aCCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCttt                                                                       <  2:1486290/93‑1 (MQ=255)
                  aCCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCttt                                                                       >  2:5845477/1‑93 (MQ=255)
                  aCCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCttt                                                                       >  2:857469/1‑93 (MQ=255)
                   ccAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTg                                                                      <  2:1115151/93‑1 (MQ=255)
                    cAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTgg                                                                     <  2:2225653/93‑1 (MQ=255)
                    cAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTgg                                                                     >  2:53206/1‑93 (MQ=255)
                    cAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTgg                                                                     >  1:1188180/1‑93 (MQ=255)
                      aaCTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGtt                                                                   <  2:593425/93‑1 (MQ=255)
                         ttGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCg                                                                <  2:5583659/93‑1 (MQ=255)
                           gTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCggg                                                              <  2:4859763/93‑1 (MQ=255)
                              aCGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTc                                                           >  1:2354067/1‑93 (MQ=255)
                               cGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCa                                                          >  2:5012953/1‑93 (MQ=255)
                                gTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCAt                                                         <  2:3351962/93‑1 (MQ=255)
                                  aCCGCGTCAAGCAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATaa                                                       <  2:5234792/93‑1 (MQ=255)
                                  aCCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATaa                                                       >  2:3080163/1‑93 (MQ=255)
                                  aCCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATaa                                                       >  1:343866/1‑93 (MQ=255)
                                  aCCGCGTCAAACAATATCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATaa                                                       <  2:6223521/93‑1 (MQ=255)
                                    cgcgTCAAACAATTTCGGCATGAAATCGAGGTATTACTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGc                                                     <  1:6230845/93‑1 (MQ=255)
                                     gcgTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGcc                                                    <  2:2620110/93‑1 (MQ=255)
                                     gcgTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGcc                                                    >  2:4554457/1‑93 (MQ=255)
                                      cgTCAAGCAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCTTAAGCCa                                                   <  1:6131299/93‑1 (MQ=255)
                                       gTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTACTGCCACTGTCCTTTGGTTGCGGGCTCATAAGCCAg                                                  >  2:3947026/1‑93 (MQ=255)
                                         cAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGac                                                <  1:2615964/93‑1 (MQ=255)
                                          aaaCAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacc                                               >  2:5156069/1‑93 (MQ=255)
                                           aaCAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacct                                              >  1:2983196/1‑93 (MQ=255)
                                           aaCAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacct                                              <  2:129741/93‑1 (MQ=255)
                                            aCAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacctt                                             >  2:3776660/1‑93 (MQ=255)
                                            aCAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacctt                                             >  2:5543173/1‑93 (MQ=255)
                                            aCAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacctt                                             >  2:1541104/1‑93 (MQ=255)
                                             cAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGaccttt                                            <  1:1462576/93‑1 (MQ=255)
                                              aaTTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGaccttta                                           >  1:4174744/1‑93 (MQ=255)
                                               aTTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacctttac                                          >  2:1259521/1‑93 (MQ=255)
                                                 ttCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacctttacct                                        <  1:1798339/93‑1 (MQ=255)
                                                 ttCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacctttacct                                        <  2:2511845/93‑1 (MQ=255)
                                                  tCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacctttacctt                                       >  1:872009/1‑93 (MQ=255)
                                                  tCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacctttacctt                                       <  1:2047558/93‑1 (MQ=255)
                                                  tCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacctttacctt                                       <  2:3686084/93‑1 (MQ=255)
                                                    ggCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGGTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTc                                     >  1:1305842/1‑93 (MQ=255)
                                                    ggCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTc                                     >  2:1030277/1‑93 (MQ=255)
                                                     gCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCg                                    >  1:1098881/1‑93 (MQ=255)
                                                      cATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGa                                   >  2:5310747/1‑93 (MQ=255)
                                                        tGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACa                                 <  1:3568041/93‑1 (MQ=255)
                                                        tGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACa                                 <  2:3493913/93‑1 (MQ=255)
                                                        tGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACa                                 >  1:5104310/1‑93 (MQ=255)
                                                        tGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACa                                 >  1:6037429/1‑93 (MQ=255)
                                                        tGAAATCGAGGTATTTCCCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACa                                 <  1:5013132/93‑1 (MQ=255)
                                                         gAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACAt                                <  1:1582909/93‑1 (MQ=255)
                                                           aaTCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGCAATGc                              >  1:5583750/1‑93 (MQ=255)
                                                            aTCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGcc                             >  2:4252517/1‑93 (MQ=255)
                                                               gAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTa                          >  2:6081782/1‑93 (MQ=255)
                                                                aGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAg                         >  2:429258/1‑93 (MQ=255)
                                                                aGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAg                         >  1:4431512/1‑93 (MQ=255)
                                                                  gTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAggt                       <  1:1763494/93‑1 (MQ=255)
                                                                   tATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGGTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAggtg                      >  1:5504050/1‑93 (MQ=255)
                                                                    aTTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAggtgg                     <  2:1003471/93‑1 (MQ=255)
                                                                    aTTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAggtgg                     >  2:3380361/1‑93 (MQ=255)
                                                                     tttCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAggtggt                    <  1:28138/93‑1 (MQ=255)
                                                                      ttttcCGTCGACCACAGCTGCTTTTCCGGCCACTGTCCTTTGGTTGCGGGATCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGtt                   <  2:3442183/90‑1 (MQ=255)
                                                                       tctcCGTCGACCCCAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGttt                  <  1:4502570/93‑1 (MQ=255)
                                                                         tcCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTc                <  1:2841165/93‑1 (MQ=255)
                                                                         tcCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTc                >  1:1060598/1‑93 (MQ=255)
                                                                         tcCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTc                >  2:6178156/1‑93 (MQ=255)
                                                                          ccGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCa               >  2:2044873/1‑93 (MQ=255)
                                                                           cGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCAt              <  1:4009585/93‑1 (MQ=255)
                                                                            gTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATa             >  1:5290578/1‑93 (MQ=255)
                                                                             tCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATAc            <  1:3653255/93‑1 (MQ=255)
                                                                             tCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATAc            <  1:4563912/93‑1 (MQ=255)
                                                                             tCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATAc            >  1:4672640/1‑93 (MQ=255)
                                                                              cGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATAcc           >  1:813122/1‑93 (MQ=255)
                                                                              cGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGATCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATAca           >  1:4244983/1‑92 (MQ=255)
                                                                                aCCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAg         >  1:5684881/1‑93 (MQ=255)
                                                                                 ccACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAgg        >  2:469363/1‑93 (MQ=255)
                                                                                 ccACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAgg        <  2:2835939/93‑1 (MQ=255)
                                                                                 ccACAGCTCCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAgg        <  2:437789/93‑1 (MQ=255)
                                                                                   acaGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGa      >  1:3053226/1‑93 (MQ=255)
                                                                                   acaGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGa      <  2:5155130/93‑1 (MQ=255)
                                                                                     aGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGAtt    <  2:5210008/93‑1 (MQ=255)
                                                                                     aGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGAtt    <  1:3873978/93‑1 (MQ=255)
                                                                                     aGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGAtt    >  2:6287099/1‑93 (MQ=255)
                                                                                          |                                                                                         
CAGCAAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGTTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCC  >  NC_000913/1652536‑1652715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑