BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,746,888	T→C	A232A (GCA→GCG)	b1669 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,746,888	0	T	C	99.5%	728.3 / ‑4.4	213	A232A (GCA→GCG)	b1669	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (124/88); total (124/89)
Fisher's exact test for biased strand distribution p-value = 4.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.50e-01

Only 100 of 224 total aligned reads displayed.

ATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTAC  >  NC_000913/1746798‑1746977

aTTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAgcgc                                                                                         <  2:3711010/93‑1 (MQ=255)

aTTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAgcgc                                                                                         <  1:2316819/93‑1 (MQ=255)

   aaGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCgcggc                                                                                      >  1:4297731/1‑93 (MQ=255)

   aaGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCgcggc                                                                                      <  2:4911776/93‑1 (MQ=255)

    aGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCgcggcg                                                                                     >  1:1844777/1‑93 (MQ=255)

    aGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCgcggcg                                                                                     >  2:2779285/1‑93 (MQ=255)

    aGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCgcggcg                                                                                     >  2:6322447/1‑93 (MQ=255)

     gACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGgcgc                                                                                    <  2:4948435/93‑1 (MQ=255)

     gACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGgcgc                                                                                    <  1:2612493/93‑1 (MQ=255)

      aCGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCt                                                                                   >  2:1998360/1‑93 (MQ=255)

        gATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGAAGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTtc                                                                                 >  2:5180842/1‑93 (MQ=255)

         aTAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTtcc                                                                                >  2:583026/1‑93 (MQ=255)

         aTAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTtcc                                                                                >  2:3426143/1‑93 (MQ=255)

         aTAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTtcc                                                                                >  1:1277469/1‑93 (MQ=255)

          tAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTtcct                                                                               >  1:3751041/1‑93 (MQ=255)

          tAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTtcct                                                                               >  2:5351901/1‑93 (MQ=255)

          tAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTtcct                                                                               >  2:2871243/1‑93 (MQ=255)

          tAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTtccc                                                                               >  1:4097383/1‑92 (MQ=255)

            gCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTtcctcc                                                                             <  2:4964760/93‑1 (MQ=255)

               gACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTgg                                                                          >  2:2358897/1‑93 (MQ=255)

               gACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTgg                                                                          >  1:1142501/1‑93 (MQ=255)

                aCTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGc                                                                         >  1:3939313/1‑93 (MQ=255)

                 cTCCGGCTCGAATATTGTTTGCGCCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGcc                                                                        <  1:6008303/93‑1 (MQ=255)

                  tCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGTGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGcccc                                                                       >  2:1135266/1‑93 (MQ=255)

                  tCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGccc                                                                       <  1:1083207/93‑1 (MQ=255)

                    cGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCga                                                                     <  1:2552302/93‑1 (MQ=255)

                     ggCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCgag                                                                    <  2:5392078/93‑1 (MQ=255)

                      gCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCgaga                                                                   <  1:4943338/93‑1 (MQ=255)

                        tCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGaat                                                                 >  1:5890342/1‑93 (MQ=255)

                        tCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGaat                                                                 >  2:547298/1‑93 (MQ=255)

                        tCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGaat                                                                 >  2:1669852/1‑93 (MQ=255)

                         cGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGaata                                                                <  2:3896379/93‑1 (MQ=255)

                         cGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGaata                                                                <  1:2297268/93‑1 (MQ=255)

                         cGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGaata                                                                >  1:1678637/1‑93 (MQ=255)

                         cGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGaata                                                                >  1:5024526/1‑93 (MQ=255)

                          gAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGaataa                                                               >  1:64881/1‑93 (MQ=255)

                           aaTATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGaataat                                                              >  2:4226993/1‑93 (MQ=255)

                             tatTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTg                                                            >  2:1847114/1‑93 (MQ=255)

                             tatTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTg                                                            >  1:3775224/1‑93 (MQ=255)

                              atTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGa                                                           <  1:860315/93‑1 (MQ=255)

                                tGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGt                                                         <  1:3449781/93‑1 (MQ=255)

                                 gTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTc                                                        <  1:4685027/93‑1 (MQ=255)

                                     tCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGAcc                                                    <  2:812597/93‑1 (MQ=255)

                                      cACCTTCAATGGCGACGGCGAAGCGACCTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCa                                                   <  1:3066267/93‑1 (MQ=255)

                                      cACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCa                                                   <  2:3258254/93‑1 (MQ=255)

                                      cACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCa                                                   <  1:366003/93‑1 (MQ=255)

                                      cACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCa                                                   >  1:2166344/1‑93 (MQ=255)

                                       aCCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCAc                                                  <  2:4328655/93‑1 (MQ=255)

                                       aCCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCAc                                                  >  2:558524/1‑93 (MQ=255)

                                       aCCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCAc                                                  >  1:4529526/1‑93 (MQ=255)

                                        ccTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACg                                                 >  1:1264498/1‑93 (MQ=255)

                                         cTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGc                                                >  2:2423916/1‑93 (MQ=255)

                                         cTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGc                                                >  1:4384633/1‑93 (MQ=255)

                                          ttCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGcc                                               >  2:2091352/1‑93 (MQ=255)

                                          ttCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGcc                                               >  1:32181/1‑93 (MQ=255)

                                          ttCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGcc                                               <  1:2380372/93‑1 (MQ=255)

                                          ttCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGcc                                               >  2:1194406/1‑93 (MQ=255)

                                          ttCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGcc                                               <  1:6397451/93‑1 (MQ=255)

                                           tCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGccc                                              >  1:2000/1‑93 (MQ=255)

                                           tCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGccc                                              <  2:5948843/93‑1 (MQ=255)

                                            cAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCg                                             >  1:3617457/1‑93 (MQ=255)

                                            cAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCg                                             >  2:2689332/1‑93 (MQ=255)

                                             aaTGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGt                                            >  2:3488881/1‑93 (MQ=255)

                                              aTGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTg                                           >  1:132630/1‑93 (MQ=255)

                                                   gACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCAc                                      >  1:4584688/1‑93 (MQ=255)

                                                   gACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCAc                                      <  2:3364533/93‑1 (MQ=255)

                                                    aCGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACt                                     >  2:4461880/1‑93 (MQ=255)

                                                    aCGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACt                                     >  2:2499562/1‑93 (MQ=255)

                                                    aCGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACt                                     >  2:4408613/1‑93 (MQ=255)

                                                       gcgaagcgaaCTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTAcc                                  >  2:4150087/1‑93 (MQ=255)

                                                        cgaagcgaaCTCTTTCGCTGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCGCGCCCGTGCTCACTACCg                                 <  1:6162426/93‑1 (MQ=255)

                                                         caagcaaaCTCTTTCGCCTGCATGATATGCCATCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGt                                <  1:2473923/92‑1 (MQ=255)

                                                          aagcgaaCTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGtt                               <  2:4044466/93‑1 (MQ=255)

                                                          aagcgaaCTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGtt                               <  1:5926900/93‑1 (MQ=255)

                                                          aagcgaaCTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGtt                               <  1:1486371/93‑1 (MQ=255)

                                                          aagcgaaCTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGtt                               >  2:6201572/1‑93 (MQ=255)

                                                            gcgaaCTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGa                             >  2:3032079/1‑93 (MQ=255)

                                                            gcgaaCTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGa                             >  2:2969563/1‑93 (MQ=255)

                                                             cgaaCTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGaa                            >  2:4583987/1‑93 (MQ=255)

                                                                aCTCTTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAAttt                         >  2:186890/1‑93 (MQ=255)

                                                                  tctTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTc                       >  1:5610654/1‑93 (MQ=255)

                                                                   ctTTCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCa                      >  2:6032115/1‑93 (MQ=255)

                                                                    tttCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCAc                     >  1:5036251/1‑93 (MQ=255)

                                                                     ttCGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCAcc                    <  1:1644169/93‑1 (MQ=255)

                                                                       cGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGc                  >  1:4829570/1‑93 (MQ=255)

                                                                       cGCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGc                  >  1:918401/1‑93 (MQ=255)

                                                                        gCCGTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCt                 <  1:255763/93‑1 (MQ=255)

                                                                           gTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGc              >  2:2227579/1‑93 (MQ=255)

                                                                           gTCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGc              >  2:1078521/1‑93 (MQ=255)

                                                                            tCATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGGTGAATTTTCACCGCTggga             >  1:3295172/1‑91 (MQ=255)

                                                                             cATGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCaa            >  1:2879840/1‑93 (MQ=255)

                                                                              aTGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAat           <  2:2188346/93‑1 (MQ=255)

                                                                              aTGATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAat           >  2:774018/1‑93 (MQ=255)

                                                                                gATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAatga         >  1:5307441/1‑93 (MQ=255)

                                                                                gATATGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAatga         >  1:3135779/1‑93 (MQ=255)

                                                                                 atatGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAatgat        <  2:5774329/93‑1 (MQ=255)

                                                                                  tatGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAatgatg       <  1:5729289/93‑1 (MQ=255)

                                                                                   atGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGAtgt      <  1:4768091/93‑1 (MQ=255)

                                                                                   atGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGAtgt      <  2:2626941/93‑1 (MQ=255)

                                                                                   atGCCAGCGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGAtgt      <  1:5257223/93‑1 (MQ=255)

ATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTAC  >  NC_000913/1746798‑1746977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`