BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	171,718	C→T	P86L (CCG→CTG)	fhuB →	FhuB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	171,718	0	C	T	100.0%	932.8 / NA	238	P86L (CCG→CTG)	fhuB	FhuB
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (112/126); total (112/126)

Only 100 of 244 total aligned reads displayed.

ACAGCTTGTTGCCGCGTCTGGCGATTTCGCTGCTGGTGGGCGCGGGTCTGGGGCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCCGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTGGGGATTACCGTCACTACGCTCTGGGCGATCCCTGGTGCGATGG  >  NC_000913/171628‑171807

aCAGCTTGTTGCCGCGTCTGGCGATTTCGCTGCTGGTGGGCGCGGGTCTGGGGCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGc                                                                                         <  2:681731/93‑1 (MQ=255)

 cAGCTTGTTGCCGCGTCTGGCGATTTCGCTGCTGGTGGGCGCGGGTCTGGGGCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCt                                                                                        <  2:2963531/93‑1 (MQ=255)

   gCTTGTTGCCGCGTCTGGCGATTTCGCTGCTGGTGGGCGCGGGTCTGGGGCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTgg                                                                                      >  2:5434963/1‑93 (MQ=255)

   gCTTGTTGCCGCGTCTGGCGATTTCGCTGCTGGTGGGCGCGGGTCTGGGGCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTgg                                                                                      >  1:3850087/1‑93 (MQ=255)

   gCTTGTTGCCGCGTCTGGCGATTTCGCTGCTGGTGGGCGCGGGTCTGGGGCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTgg                                                                                      >  1:1570380/1‑93 (MQ=255)

     ttgttgCCGCGTCTGGCGATTTCGCTGCTGGTGGGCGCGGGTCTGGGGCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCg                                                                                    <  2:5400225/93‑1 (MQ=255)

      tgttgCCGCGTCTGGCGATTTCGCTGCTGGTGGGCGCGGGTCTGGGGCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCgg                                                                                   >  1:4242176/1‑93 (MQ=255)

      tgttgCCGCGTCTGGCGATTTCGCTGCTGGTGGGCGCGGGTCTGGGGCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCgg                                                                                   <  1:2729462/93‑1 (MQ=255)

       gttgCCGCGTCTGGCGATTTCGCTGCTGGTGGGCGCGGGTCTGGGGCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGa                                                                                  >  1:444226/1‑93 (MQ=255)

        ttgCCGCGTCTGGCGATTTCGCTGCTGGTGGGCGCGGGTCTGGGGCTGGTGGGCGTGCTGTTTCAGCAAGGGCTGCGTAACCTGCTGGCGGAg                                                                                 >  2:4096230/1‑93 (MQ=255)

             gcgTCTGGCGATTTCGCTGCTGGTGGGCGCGGGTCTGGGGCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCcgac                                                                            >  2:2140478/1‑93 (MQ=255)

             gcgTCTGGCGATTTCGCTGCTGGTGGGCGCGGGTCTGGGGCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCcgac                                                                            >  1:3392411/1‑93 (MQ=255)

             gcgTCTGGCGATTTCGCTGCTGGTGGGCGCGGGTCTGGGGCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCcgac                                                                            <  1:4984131/93‑1 (MQ=255)

              cgTCTGGCGATTTCGCTGCTGGTGGGCGCGGGTCTGGGGCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCcgacg                                                                           >  1:805176/1‑93 (MQ=255)

              cgTCTGGCGATTTCGCTGCTGGTGGGCGCGAGTCTGGGGCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTACCCTGCTGGCGGAGCcgacg                                                                           <  1:3151629/93‑1 (MQ=255)

                   ggCGATTTCGCTGCTGGTGGGCGCGGGTCTGGGGCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCt                                                                      >  2:1666315/1‑93 (MQ=255)

                   ggCGATTTCGCTGCTGGTGGGCGCGGGTCTGGGGCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCt                                                                      >  1:2886974/1‑93 (MQ=255)

                    gCGATTTCGCTGCTGGTGGGCGCGGGTCTGGGGCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCtt                                                                     <  2:2780757/93‑1 (MQ=255)

                    gCGATTTCGCTGCTGGTGGGCGCGGGTCTGGGGCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCtt                                                                     <  2:2768630/93‑1 (MQ=255)

                    gCGATTTCGCTGCTGGTGGGCGCGGGTCTGGGGCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCtg                                                                     >  1:5100362/1‑92 (MQ=255)

                     cGATTTCGCTGCTGGTGGGCGCGGGTCTGGGGCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTg                                                                    <  1:1654112/93‑1 (MQ=255)

                     cGATTTCGCTGCTGGTGGGCGCGGGTCTGGGGCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTg                                                                    <  2:4509977/93‑1 (MQ=255)

                     cGATTTCGCTGCTGGTGGGCGCGGGTCTGGGGCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTg                                                                    <  1:3126143/93‑1 (MQ=255)

                      gATTTCGCTGCTGGTGGGCGCGGGTCTGGGGCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTgg                                                                   <  1:1127719/93‑1 (MQ=255)

                      gATTTCGCTGCTGGTGGGCGCGGGTCTGGGGCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTgg                                                                   >  1:6108158/1‑93 (MQ=255)

                      gATTTCGCTGCTGGTGGGCGCGGGTCTGGGGCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTgg                                                                   <  2:1703497/93‑1 (MQ=255)

                       aTTTCGCTGCTGGTGGGCGCGGGTCTGGGGCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGc                                                                  >  2:1638586/1‑93 (MQ=255)

                       aTTTCGCTGCTGGTGGGCGCGGGTCTGGGGCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGc                                                                  >  1:569544/1‑93 (MQ=255)

                         ttCGCTGCTGGTGGGCGCGGGTCTGGGGCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGt                                                                <  1:4481333/93‑1 (MQ=255)

                          tCGCTGCTGGTGGGCGCGGGTCTGGGGCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGtt                                                               <  2:4802589/93‑1 (MQ=255)

                           cGCTGCTGGTGGGCGCGGGTCTGGGGCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTg                                                              >  2:6120407/1‑93 (MQ=255)

                            gctgctGGTGGGCGCGGGTCTGGGGCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGc                                                             <  2:4393307/93‑1 (MQ=255)

                             ctgctgGTGGGCGCGGGTCTGGGGCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCt                                                            >  1:854891/1‑93 (MQ=255)

                             ctgctgGTGGGCGCGGGTCTGGGGCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCt                                                            <  1:6035541/93‑1 (MQ=255)

                                ctgGTGGGCGCGGGTCTGGGGCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACa                                                         <  1:2444930/93‑1 (MQ=255)

                                 tggtggGCGCGGGTCTGGGGCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAg                                                        >  1:5791634/1‑93 (MQ=255)

                                 tggtggGCGCGGGTCTGGGGCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAg                                                        <  2:6384297/93‑1 (MQ=255)

                                 tggtggGCGCGGGTCTGGGGCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAg                                                        <  1:1836785/93‑1 (MQ=255)

                                 tggtggGCGCGGGTCTGGGGCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAg                                                        <  2:4085623/93‑1 (MQ=255)

                                 gggggggCGCGGGTCTGGGGCTGGTGGGCGTGCGGTTGCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAg                                                        <  2:5001643/89‑1 (MQ=255)

                                   gtggGCGCGGGTCTGGGGCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGgc                                                      <  2:4843993/93‑1 (MQ=255)

                                        cgcgGGTCTGGGGCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCa                                                 >  2:2490193/1‑93 (MQ=255)

                                         gcgGGTCTGGGGCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCTCGCaa                                                <  2:5681755/93‑1 (MQ=255)

                                           gggTCTGGGGCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACt                                              >  2:3920177/1‑93 (MQ=255)

                                             gTCTGGGGCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTgg                                            >  2:1291719/1‑93 (MQ=255)

                                              tCTGGGGCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTggg                                           <  1:1544995/93‑1 (MQ=255)

                                              tCTGGGGCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTggg                                           <  2:4669212/93‑1 (MQ=255)

                                               cTGGGGCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTgggg                                          >  1:5847065/1‑93 (MQ=255)

                                               cTGGGGCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTgggg                                          <  1:1721406/93‑1 (MQ=255)

                                                tGGGGCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTAGAGGCGCGCAACTGGGGa                                         >  1:1031157/1‑93 (MQ=255)

                                                tGGGGCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTGGGGa                                         >  1:4331755/1‑93 (MQ=255)

                                                 ggggCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTggggct                                        >  1:5256937/1‑91 (MQ=255)

                                                 ggggCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTGGGGAt                                        <  1:4723554/93‑1 (MQ=255)

                                                 ggggCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTGGGGAt                                        >  2:6334478/1‑93 (MQ=255)

                                                 ggggCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTGGGGAt                                        <  2:3504011/93‑1 (MQ=255)

                                                  gggCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTGGGGAtt                                       <  1:3584230/93‑1 (MQ=255)

                                                     cTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTGGGGATTAcc                                    >  2:4449359/1‑93 (MQ=255)

                                                      tggtggGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGCCGACGCTTGGCGTTGCTACAGGCGCGCAACTGGGGATTACCg                                   >  2:1962169/1‑93 (MQ=255)

                                                      tggtggGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTGGGGATTACCg                                   >  2:5202872/1‑93 (MQ=255)

                                                       ggtggGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTGGGGATTACCGt                                  >  2:4609512/1‑93 (MQ=255)

                                                        gtggGCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTGGGGATTACCGTc                                 <  1:2692582/93‑1 (MQ=255)

                                                          gggCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTGGGGATTACCGTCAc                               >  2:4962331/1‑93 (MQ=255)

                                                            gCGTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTGGGGATTACCGTCACTa                             >  2:3138257/1‑93 (MQ=255)

                                                              gTGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTGGGGATTACCGTCACTACg                           >  1:2645491/1‑93 (MQ=255)

                                                               tGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTGGGGATTACCGTCACTACGc                          <  2:3232175/93‑1 (MQ=255)

                                                               tGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTGGGGATTACCGTCACTACGc                          <  1:1410517/93‑1 (MQ=255)

                                                               tGCTGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTGGGGATTACCGTCACTACGc                          <  2:2881948/93‑1 (MQ=255)

                                                               tGCTGTTTCAGAAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTGGGGATTACCGTCACTACGc                          <  1:2257412/93‑1 (MQ=255)

                                                                  tGTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTGGGGATTACCGTCACTACGctct                       >  1:747592/1‑93 (MQ=255)

                                                                   gTTTCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTGGGGATTACCGTCACTACGCTCTg                      >  1:6539/1‑93 (MQ=255)

                                                                    tttCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTGGGGATTACCGTCACTACGCTCTgg                     >  1:5517854/1‑93 (MQ=255)

                                                                     ttCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTGGGGATTACCGTCACTACGCTCTggg                    <  2:2623441/93‑1 (MQ=255)

                                                                     ttCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTGGGGATTACCGTCACTACGCTCTggg                    >  2:3867955/1‑93 (MQ=255)

                                                                      tCAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTGGGGATTACCGTCACTACGCTCTGGGc                   >  1:4679134/1‑93 (MQ=255)

                                                                       cAGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTGGGGATTACCGTCACTACGCTCTGGGCg                  >  2:1085717/1‑93 (MQ=255)

                                                                        aGCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTGGGGATTACCGTCACTACGCTCTGGGCGa                 <  1:5445973/93‑1 (MQ=255)

                                                                         gCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTGGGGATTACCGTCACTACGCTCTGGGCGAt                <  1:1221307/93‑1 (MQ=255)

                                                                         gCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTGGGGATTACCGTCACTACGCTCTGGGCGAt                >  2:3311448/1‑93 (MQ=255)

                                                                         gCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTGGGGATTACCGTCACTACGCTCTGGGCGAt                >  1:6118061/1‑93 (MQ=255)

                                                                         gCAAGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTGGGGATTACCGTCACTACGCTCTGGGCGAt                <  2:3769136/93‑1 (MQ=255)

                                                                           aaGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTGGGGATTACCGTCACTACGCTCTGGGCGATcc              >  1:4365206/1‑93 (MQ=255)

                                                                            aGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTGGGGATTACCGTCACTACGCTCTGGGCGATccc             <  2:1923794/93‑1 (MQ=255)

                                                                            aGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTGGGGATTACCGTCACTACGCTCTGGGCGATccc             <  2:1824/93‑1 (MQ=255)

                                                                            aGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTGGGGATTACCGTCACTACGCTCTGGGCGATccc             >  1:2982494/1‑93 (MQ=255)

                                                                            aGTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTGGGGATTACCGTCACTACGCTCTGGGCGATccc             <  1:3032774/93‑1 (MQ=255)

                                                                             gTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTGGGGATTACCGTCACTACGCTCTGGGCGATCCCt            >  1:4926616/1‑93 (MQ=255)

                                                                             gTGCTGCGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTGGGGATTACCGTCACTACGCTCTGGGCGATCCCt            <  1:3695211/93‑1 (MQ=255)

                                                                              tgctgcGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTGGGGATTACCGTCACTACGCTCTGGGCGATCCCTg           <  1:936177/93‑1 (MQ=255)

                                                                              tgctgcGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTGGGGATTACCGTCACTACGCTCTGGGCGATCCCTg           <  2:753653/93‑1 (MQ=255)

                                                                               gctgcGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTGGGGATTACCGTCACTACGCTCTGGGCGATCCCTgg          <  1:4142486/93‑1 (MQ=255)

                                                                               gctgcGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTGGGGATTACCGTCACTACGCTCTGGGCGATCCCTgg          <  2:1158235/93‑1 (MQ=255)

                                                                                 tgcGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTGGGGATTACCGTCACTACGCTCTGGGCGATCCCTGGTg        <  2:1996080/93‑1 (MQ=255)

                                                                                  gcGTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTGGGGATTACCGTCACTACGCTCTGGGCGATCCCTGGTGc       <  2:517838/93‑1 (MQ=255)

                                                                                    gTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTGGGGATTACCGTCACTACGCTCTGGGCGATCCCTGGTGCGa     >  1:216713/1‑93 (MQ=255)

                                                                                    gTAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTGGGGATTACCGTCACTACGCTCTGGGCGATCCCTGGTGCGa     >  1:5974758/1‑93 (MQ=255)

                                                                                     tAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTGGGGATTACCGTCACTACGCTCTGGGCGATCCCTGGTGCGAt    <  2:6230327/93‑1 (MQ=255)

                                                                                     tAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTGGGGATTACCGTCACTACGCTCTGGGCGATCCCTGGTGCGAt    <  1:474927/93‑1 (MQ=255)

                                                                                     tAACCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTGGGGATTACCGTCACTACGCTCTGGGCGATCCCTGGTGCGAt    <  1:3943564/93‑1 (MQ=255)

                                                                                       aCCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTGGGGATTACCGTCACTACGCTCTGGGCGATCCCTGGTGCGATgg  >  1:977642/1‑93 (MQ=255)

                                                                                       aCCTGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTGGGGATTACCGTCACTACGCTCTGGGAGATCCCTGGTGCGAtag  >  1:2545643/1‑91 (MQ=255)

ACAGCTTGTTGCCGCGTCTGGCGATTTCGCTGCTGGTGGGCGCGGGTCTGGGGCTGGTGGGCGTGCTGTTTCAGCAAGTGCTGCGTAACCCGCTGGCGGAGCCGACGACGCTTGGCGTTGCTACAGGCGCGCAACTGGGGATTACCGTCACTACGCTCTGGGCGATCCCTGGTGCGATGG  >  NC_000913/171628‑171807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`