BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,860,370	T→C	E28E (GAA→GAG)	yeaA ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,860,370	0	T	C	99.6%	788.8 / NA	226	E28E (GAA→GAG)	yeaA	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (130/95); minor base A (1/0); total (131/95)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 238 total aligned reads displayed.

GGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGTTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCT  >  NC_000913/1860280‑1860458

ggTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTTTTATGCAGTAAACAACCCGCAAATGGCGGCTc                                                                                        >  1:4023541/1‑93 (MQ=255)

ggTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTc                                                                                        <  1:591700/93‑1 (MQ=255)

ggTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTc                                                                                        >  2:1097605/1‑93 (MQ=255)

ggTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTc                                                                                        <  1:5815119/93‑1 (MQ=255)

    tGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTc                                                                                    >  2:6436154/1‑93 (MQ=255)

     gggAATGAAACAGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTcc                                                                                   <  2:6025098/93‑1 (MQ=255)

     gggAATGAAACAGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTcc                                                                                   >  1:2831384/1‑93 (MQ=255)

     gggAATGAAACAGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTcc                                                                                   >  2:5648631/1‑93 (MQ=255)

      ggAATGAAACAGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTccc                                                                                  <  2:2758182/93‑1 (MQ=255)

      ggAATGAAACAGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTccc                                                                                  >  2:2476941/1‑93 (MQ=255)

        aaTGAAACAGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCAt                                                                                >  2:353615/1‑93 (MQ=255)

         aTGAAACAGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATg                                                                               >  1:4572467/1‑93 (MQ=255)

          tGAAACAGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGa                                                                              >  2:4420621/1‑93 (MQ=255)

            aaaCAGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGAtt                                                                            >  1:1191007/1‑93 (MQ=255)

             aaCAGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTc                                                                           >  2:5721687/1‑93 (MQ=255)

              aCAGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCt                                                                          >  2:2856722/1‑93 (MQ=255)

                 gCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCg                                                                       >  1:2692646/1‑93 (MQ=255)

                 gCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCg                                                                       <  2:1564792/93‑1 (MQ=255)

                 gCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCg                                                                       >  1:3431907/1‑93 (MQ=255)

                  cGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGt                                                                      >  2:4577624/1‑93 (MQ=255)

                   ggggCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTc                                                                     >  1:2555579/1‑93 (MQ=255)

                   ggggCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTc                                                                     >  2:3356437/1‑93 (MQ=255)

                    gggCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCa                                                                    >  2:2292001/1‑93 (MQ=255)

                        aTCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTa                                                                >  2:6458082/1‑93 (MQ=255)

                        aTCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTa                                                                >  2:3466513/1‑93 (MQ=255)

                        aTCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTa                                                                >  1:4747345/1‑93 (MQ=255)

                           gCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTaaaa                                                             >  1:6336799/1‑93 (MQ=255)

                           gCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTaaaa                                                             >  1:3865486/1‑93 (MQ=255)

                             aGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACt                                                           >  2:5768100/1‑93 (MQ=255)

                              gATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTg                                                          >  2:3432567/1‑93 (MQ=255)

                               aTCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGc                                                         >  1:4725463/1‑93 (MQ=255)

                               aTCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGc                                                         >  1:5499189/1‑93 (MQ=255)

                               aTCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGc                                                         >  1:1788112/1‑93 (MQ=255)

                               aTCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGc                                                         <  1:1110514/93‑1 (MQ=255)

                                tCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCa                                                        >  2:4962730/1‑93 (MQ=255)

                                tCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCa                                                        >  2:5145969/1‑93 (MQ=255)

                                  aaaCAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCAtc                                                      >  1:697786/1‑93 (MQ=255)

                                   aaCAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCAtct                                                     <  1:3623613/93‑1 (MQ=255)

                                   aaCAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCAtct                                                     <  1:3188857/93‑1 (MQ=255)

                                   aaCAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCAtct                                                     >  2:2015557/1‑93 (MQ=255)

                                   aaCAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCAtct                                                     >  1:1277552/1‑93 (MQ=255)

                                     cAGGGATATACGCCGTCACGCTTGTTATGCAGTAAACGAACCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCg                                                   >  1:965737/1‑93 (MQ=255)

                                      aGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCgg                                                  <  2:947466/93‑1 (MQ=255)

                                       gTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGa                                                 >  2:1285991/1‑93 (MQ=255)

                                       gTGATATACGCCATCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGa                                                 >  1:2980164/1‑93 (MQ=255)

                                             taCGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAAtt                                           <  2:1369599/93‑1 (MQ=255)

                                              aCGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAAttt                                          >  2:5205271/1‑93 (MQ=255)

                                              aCGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAAttt                                          >  1:2203665/1‑93 (MQ=255)

                                              aCGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATACTGCGTCACGTAAAACTGCATCTCGGACAAAttt                                          <  2:2589442/93‑1 (MQ=255)

                                                gCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAAttttt                                        >  1:3522545/1‑93 (MQ=255)

                                                 ccGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAAtttttt                                       >  2:1280622/1‑93 (MQ=255)

                                                 ccGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAAtttttt                                       >  2:1909737/1‑93 (MQ=255)

                                                  cGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAAttttttt                                      <  1:3244045/93‑1 (MQ=255)

                                                   gTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAAtttttttt                                     >  2:817935/1‑93 (MQ=255)

                                                    tCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTc                                    >  1:770304/1‑93 (MQ=255)

                                                    tCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTc                                    <  1:4109780/93‑1 (MQ=255)

                                                     cACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCa                                   >  2:6343536/1‑93 (MQ=255)

                                                     cACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCa                                   >  2:4728606/1‑93 (MQ=255)

                                                     cACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCa                                   >  1:6142620/1‑93 (MQ=255)

                                                      aCGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTATCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTATTCAg                                  >  2:1591476/1‑93 (MQ=255)

                                                       cGCTTGTTATGCAGTAAACGCCCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGt                                 <  2:3200632/93‑1 (MQ=255)

                                                       cGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGt                                 >  1:5954961/1‑93 (MQ=255)

                                                        gCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGtt                                <  1:4069159/93‑1 (MQ=255)

                                                        gCTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGtt                                >  2:1500154/1‑93 (MQ=255)

                                                         cTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGttc                               >  2:5799428/1‑93 (MQ=255)

                                                         cTTGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGttc                               <  2:1220627/93‑1 (MQ=255)

                                                          ttGTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGttct                              <  1:3744011/93‑1 (MQ=255)

                                                            gTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGttcttc                            <  1:5296947/93‑1 (MQ=255)

                                                            gTTATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGttcttc                            >  1:4999895/1‑93 (MQ=255)

                                                             ttATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTtcttct                           <  1:4580257/93‑1 (MQ=255)

                                                             ttATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTtcttct                           <  1:1345336/93‑1 (MQ=255)

                                                             ttATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTtcttct                           <  2:3649835/93‑1 (MQ=255)

                                                             ttATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTtcttct                           >  2:772413/1‑93 (MQ=255)

                                                              tATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTg                          >  2:2840853/1‑93 (MQ=255)

                                                              tATGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTg                          <  1:2634684/93‑1 (MQ=255)

                                                               aTGCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGc                         <  1:3693694/93‑1 (MQ=255)

                                                                 gCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCg                       >  2:3760583/1‑93 (MQ=255)

                                                                 gCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCg                       >  2:424445/1‑93 (MQ=255)

                                                                 gCAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCg                       >  2:2625121/1‑93 (MQ=255)

                                                                  cAGTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGa                      <  2:3849558/93‑1 (MQ=255)

                                                                   aGTAACCGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGaa                     <  1:1904920/93‑1 (MQ=255)

                                                                    gTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAg                    >  2:1664775/1‑93 (MQ=255)

                                                                    gTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAg                    <  1:1375850/93‑1 (MQ=255)

                                                                    gTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAg                    <  2:5448631/93‑1 (MQ=255)

                                                                    gTAAACGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAg                    <  1:5632533/93‑1 (MQ=255)

                                                                       aaCGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGtt                 <  1:2338687/93‑1 (MQ=255)

                                                                        aCGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTACAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGCAGGctt                >  1:3486514/1‑93 (MQ=255)

                                                                        aCGACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGttt                >  2:4833770/1‑93 (MQ=255)

                                                                          gACCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTttat              >  1:4820099/1‑93 (MQ=255)

                                                                           aCCCGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTttatt             >  1:2782990/1‑93 (MQ=255)

                                                                             ccGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAg           <  2:1785583/93‑1 (MQ=255)

                                                                             ccGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAg           <  2:29955/93‑1 (MQ=255)

                                                                             ccGTAAATGGCGGCTATGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAg           <  2:2043716/93‑1 (MQ=255)

                                                                              cGTAAATGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGc          <  1:1015457/93‑1 (MQ=255)

                                                                                  aaTGGCGGCTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGAGAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCAtt      <  2:4529231/93‑1 (MQ=255)

                                                                                  aaTGGCGGATCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCAtt      >  1:569215/1‑93 (MQ=255)

                                                                                     ggcggcTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGc   <  1:2484251/93‑1 (MQ=255)

                                                                                     ggcggcTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGc   <  2:526663/93‑1 (MQ=255)

                                                                                     ggcggcTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGc   <  2:220344/93‑1 (MQ=255)

                                                                                     ggcggcTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGc   <  1:6088559/93‑1 (MQ=255)

GGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACGCTTGTTATGCAGTAAACGACCCGTAAATGGCGGTTCTGTCCCATGATTCTGCGTCACGTAAAACTGCATCTCGGACAAATTTTTTTTCAGTTCTTCTGCCGAAGGTTTATTAGCCATTTGCT  >  NC_000913/1860280‑1860458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`