BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,862,301	T→C	V143A (GTG→GCG)	yeaD →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,862,301	0	T	C	99.5%	656.5 / ‑5.5	189	V143A (GTG→GCG)	yeaD	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (88/100); total (88/101)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 200 total aligned reads displayed.

ATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATC  >  NC_000913/1862211‑1862389

 tGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCggg                                                                                       <  1:5910820/93‑1 (MQ=255)

 tGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCggg                                                                                       <  2:2783683/93‑1 (MQ=255)

  tgCGTAGCGCTGACTTCTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGt                                                                                      <  2:17669/92‑1 (MQ=255)

  ggCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGt                                                                                      >  2:1176048/1‑93 (MQ=255)

   gCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTa                                                                                     >  1:4828478/1‑93 (MQ=255)

   gCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTa                                                                                     >  2:4876100/1‑93 (MQ=255)

     gTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTaaa                                                                                   >  1:34560/1‑93 (MQ=255)

        gcgcTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACt                                                                                >  1:5669426/1‑93 (MQ=255)

        gcgcTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACt                                                                                >  2:165275/1‑93 (MQ=255)

        gcgcTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACt                                                                                >  2:1834171/1‑93 (MQ=255)

           cTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTtgt                                                                             >  1:1839183/1‑93 (MQ=255)

            tGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTtgtg                                                                            <  2:3833637/93‑1 (MQ=255)

             gACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGa                                                                           >  1:999224/1‑93 (MQ=255)

              aCTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGaa                                                                          >  2:6393383/1‑93 (MQ=255)

               cTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGaaa                                                                         <  2:1330384/93‑1 (MQ=255)

                   tGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCAGGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGa                                                                     >  2:4527935/1‑93 (MQ=255)

                   tGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGa                                                                     >  1:2918719/1‑93 (MQ=255)

                   tGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGa                                                                     >  2:5849931/1‑93 (MQ=255)

                   tGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGa                                                                     <  1:5788287/93‑1 (MQ=255)

                   tGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGCGTAAAACTTGTGAAATCGa                                                                     <  1:6298284/93‑1 (MQ=255)

                     aaTTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATc                                                                   <  2:4361998/93‑1 (MQ=255)

                     aaTTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATc                                                                   <  2:6246936/93‑1 (MQ=255)

                     aaTTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATc                                                                   <  2:410812/93‑1 (MQ=255)

                     aaTTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATc                                                                   <  1:2030717/93‑1 (MQ=255)

                      aTTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCt                                                                  >  1:4722532/1‑93 (MQ=255)

                      aTTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCt                                                                  <  1:2673783/93‑1 (MQ=255)

                       ttGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCtt                                                                 <  1:3975485/93‑1 (MQ=255)

                       ttGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCtt                                                                 >  1:2308588/1‑93 (MQ=255)

                       atGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCtt                                                                 <  1:3661885/92‑1 (MQ=255)

                        tGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTg                                                                <  2:4619791/93‑1 (MQ=255)

                        tGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTg                                                                <  2:2653096/93‑1 (MQ=255)

                         gACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGa                                                               >  1:4012707/1‑93 (MQ=255)

                         gACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGa                                                               >  1:5746324/1‑93 (MQ=255)

                               aaGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATcaca                                                         <  1:792595/93‑1 (MQ=255)

                                 gCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATg                                                       <  2:1516323/93‑1 (MQ=255)

                                 gCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATg                                                       <  2:5105456/93‑1 (MQ=255)

                                 gCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATg                                                       >  2:4485080/1‑93 (MQ=255)

                                  cGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATgg                                                      <  2:1044868/93‑1 (MQ=255)

                                  cGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATgg                                                      >  2:4226971/1‑93 (MQ=255)

                                   gAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGc                                                     <  2:2241272/93‑1 (MQ=255)

                                      gagaCGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGaa                                                  <  2:794768/93‑1 (MQ=255)

                                      gagaCGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGaa                                                  >  1:1886450/1‑93 (MQ=255)

                                      gagaCGAAAAAATTCTCGTCGCACGACTTTACGCTGTTAGTGAATTTTCGCGCGGGGAAAACTTGTGAAATCGATCTTGCATCATACTGCGaa                                                  >  2:2851315/1‑93 (MQ=255)

                                        gaCGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAAtt                                                <  2:1192935/93‑1 (MQ=255)

                                        gaCGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAAtt                                                <  2:5774320/93‑1 (MQ=255)

                                        gaCGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAAtt                                                >  2:4850336/1‑93 (MQ=255)

                                        gaCGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAAtt                                                >  2:3205262/1‑93 (MQ=255)

                                         aCGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAAttt                                               >  1:6214436/1‑93 (MQ=255)

                                         aCGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAAttt                                               <  1:442768/93‑1 (MQ=255)

                                         aCGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAAttt                                               >  1:720602/1‑93 (MQ=255)

                                            aaaaaaTTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGaa                                            >  2:3013538/1‑93 (MQ=255)

                                             aaaaaTTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGaaa                                           <  1:3416304/93‑1 (MQ=255)

                                              aaaaTTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAac                                          <  2:3358762/93‑1 (MQ=255)

                                              aaaaTTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAac                                          >  1:1495575/1‑93 (MQ=255)

                                              aaaaTTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAac                                          <  1:2123313/93‑1 (MQ=255)

                                               aaaTTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAacc                                         <  1:4134881/93‑1 (MQ=255)

                                                    cTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACctc                                    <  1:3508176/93‑1 (MQ=255)

                                                     tGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACctct                                   <  1:6038430/93‑1 (MQ=255)

                                                      ggCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTg                                  >  2:5333393/1‑93 (MQ=255)

                                                       gCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGc                                 >  1:5314361/1‑93 (MQ=255)

                                                         cGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTTAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGccc                               >  2:4799334/1‑93 (MQ=255)

                                                          gCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCt                              <  1:3195589/93‑1 (MQ=255)

                                                          gCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCt                              <  2:5190054/93‑1 (MQ=255)

                                                           cACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTg                             >  2:2038060/1‑93 (MQ=255)

                                                            aCGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGc                            >  1:273751/1‑93 (MQ=255)

                                                            aCGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGc                            <  1:3797599/93‑1 (MQ=255)

                                                            aCGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGc                            >  1:3860418/1‑93 (MQ=255)

                                                             cGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCa                           >  2:1436070/1‑93 (MQ=255)

                                                              gACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCAt                          <  2:6273470/93‑1 (MQ=255)

                                                               aCTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATa                         >  1:152763/1‑93 (MQ=255)

                                                                 tttACGCTATTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATAcc                       >  2:1923210/1‑93 (MQ=255)

                                                                  ttACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGGAACCACCTCTGCCCTGCATACCt                      >  2:670570/1‑93 (MQ=255)

                                                                  ttACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCt                      <  1:302752/93‑1 (MQ=255)

                                                                   tACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTa                     >  1:4302264/1‑93 (MQ=255)

                                                                      gCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACtt                  >  2:187377/1‑93 (MQ=255)

                                                                       cTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACttt                 <  2:6334089/93‑1 (MQ=255)

                                                                       cTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACttt                 <  1:1446706/93‑1 (MQ=255)

                                                                        tGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTa                >  1:820343/1‑93 (MQ=255)

                                                                         gTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTaa               <  1:4696802/93‑1 (MQ=255)

                                                                          ttAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAAc              <  1:2508939/93‑1 (MQ=255)

                                                                            aGCGCATTTCCGCGCGGGTAAATCTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGt            <  1:6365759/93‑1 (MQ=255)

                                                                              cgcATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTgg          <  1:5452887/93‑1 (MQ=255)

                                                                              cgcATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTgg          >  2:5585561/1‑93 (MQ=255)

                                                                               gcATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCCCCTCTGCCCTGCATACCTACTTTAACGTggg         <  1:4453398/93‑1 (MQ=255)

                                                                               gcATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTggg         >  2:1440546/1‑93 (MQ=255)

                                                                               gcATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTggg         >  2:5008536/1‑93 (MQ=255)

                                                                                cATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTgggg        >  1:4390317/1‑92 (MQ=255)

                                                                                cATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGt        <  2:2189003/93‑1 (MQ=255)

                                                                                cATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGt        >  1:1987275/1‑93 (MQ=255)

                                                                                cATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGt        <  1:5419184/93‑1 (MQ=255)

                                                                                 aTTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTg       <  2:880846/93‑1 (MQ=255)

                                                                                 aTTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTg       <  2:741162/93‑1 (MQ=255)

                                                                                 aTTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTg       >  1:4921409/1‑93 (MQ=255)

                                                                                 aTTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTg       <  1:6119613/93‑1 (MQ=255)

                                                                                  tttCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGa      >  1:519007/1‑93 (MQ=255)

                                                                                  tttCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGa      >  1:644360/1‑93 (MQ=255)

                                                                                  tttCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGa      >  1:1565826/1‑93 (MQ=255)

                                                                                    tCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGata    <  2:417714/93‑1 (MQ=255)

                                                                                      cgcgcgGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATc  <  1:1080218/93‑1 (MQ=255)

                                                                                      cgcgcgGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATc  <  2:408900/93‑1 (MQ=255)

ATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATC  >  NC_000913/1862211‑1862389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`