BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,094,891	T→C	L86L (TTA→CTA)	hisI →	phosphoribosyl‑amp cyclohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,094,891	0	T	C	100.0%	667.8 / NA	195	L86L (TTA→CTA)	hisI	phosphoribosyl‑amp cyclohydrolase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (113/82); total (113/82)

Only 100 of 220 total aligned reads displayed.

GCACTAAACAGCGACTGTGGACCAAAGGCGAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGTTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGCACCAGCAGTTGCTTCGGCGACACCGCTCACCAGTGGCTGTTCCTG  >  NC_000913/2094802‑2094980

 cACTAAACAGCGACTGTGGACCAAAGGCGAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACt                                                                                       <  1:3792204/93‑1 (MQ=255)

  aCTAAACAGCGACTGTTGACCAAAGGCGAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTg                                                                                      <  1:1371760/93‑1 (MQ=255)

    tAAACAGCGACTGTGGACCAAAGGCGAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGt                                                                                    <  2:632628/93‑1 (MQ=255)

    tAAACAGCGACGGTGGACCAAAGGCGAAGCGTCGGACAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGt                                                                                    <  1:2496302/93‑1 (MQ=255)

       aCAGCGACTGTGGACCAAAGGCGAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCt                                                                                 >  1:4372149/1‑93 (MQ=255)

       aCAGCGACTGTGGACCAAAGGCGAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCt                                                                                 >  2:1618378/1‑93 (MQ=255)

        cAGCGACTGTGGACCAAAGGCGAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTg                                                                                <  1:5230869/93‑1 (MQ=255)

        cAGCGACTGTGGACCAAAGGCGAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTg                                                                                <  1:2828288/93‑1 (MQ=255)

         aGCGACTGTGGACCAAAGGCGAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTgg                                                                               >  2:5659072/1‑93 (MQ=255)

         aGCGACTGTGGACCAAAGGCGAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTgg                                                                               >  2:2176723/1‑93 (MQ=255)

          gCGACTGTGGACCAAAGGCGAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGc                                                                              >  2:5324011/1‑93 (MQ=255)

          gCGACTGTGGACCAAAGGCGAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGc                                                                              >  1:1869649/1‑93 (MQ=255)

           cGACTGTGGACCAAAGGCGAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGTCACGCTACTGGTGCTGGCg                                                                             <  1:6239216/93‑1 (MQ=255)

           cGACTGTGGACCAAAGGCGAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCg                                                                             <  1:3617921/93‑1 (MQ=255)

            gACTGTGGACCAAAGGCGAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGa                                                                            >  1:2658709/1‑93 (MQ=255)

            gACTGTGGACCAAAGGCGAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGa                                                                            >  1:1081469/1‑93 (MQ=255)

             aCTGTGGACCAAAGGCGAAACGTCGGGCAATTTGCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGaa                                                                           <  1:5970719/93‑1 (MQ=255)

             aCTGTGGACCAAAGGCGAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGaa                                                                           >  2:2353690/1‑93 (MQ=255)

               tgtgGACCAAAGGCGAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATc                                                                         >  2:6223344/1‑93 (MQ=255)

               tgtgGACCAAAGGCGAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATc                                                                         <  1:630497/93‑1 (MQ=255)

                 tgGACCAAAGGCGAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATccc                                                                       <  1:1781356/93‑1 (MQ=255)

                  ggACCAGAGGCGAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCa                                                                      >  1:5357650/1‑93 (MQ=255)

                  ggACCAAAGGCGAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCa                                                                      >  2:4227292/1‑93 (MQ=255)

                  ggACCAAAGGCGAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCa                                                                      <  2:1055158/93‑1 (MQ=255)

                   gACCAAAGGCGAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCAt                                                                     >  1:5271187/1‑93 (MQ=255)

                   gACCAAAGGCGAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCAt                                                                     >  2:5495372/1‑93 (MQ=255)

                   gACCAAAGGCGAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCAt                                                                     >  2:1364903/1‑93 (MQ=255)

                    aCCAAAGGCGAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACCCGCTACTGGTGCTGGCGAATCCCATc                                                                    <  1:6260133/93‑1 (MQ=255)

                    aCCAAAGGCGAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATc                                                                    <  1:3331420/93‑1 (MQ=255)

                      cAAAGGCGAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCgg                                                                  >  2:6106313/1‑93 (MQ=255)

                       aaaGGCGAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGc                                                                 >  2:4406110/1‑93 (MQ=255)

                       aaaGGCGAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGc                                                                 <  2:909869/93‑1 (MQ=255)

                        aaGGCGAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGcc                                                                >  2:5100324/1‑93 (MQ=255)

                        aaGGCGAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGcc                                                                <  1:5566746/93‑1 (MQ=255)

                        aaGGCGAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGcc                                                                <  1:4584561/93‑1 (MQ=255)

                        aaGGCGAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGcc                                                                <  2:5785703/93‑1 (MQ=255)

                        aaGGCGAAACGTCGGGCAATTTCCTCAACGAAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGcc                                                                >  1:931443/1‑93 (MQ=255)

                         aGGCGAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGccc                                                               <  2:3176568/93‑1 (MQ=255)

                          ggCGAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGCCCg                                                              >  2:3302765/1‑93 (MQ=255)

                             gAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGgcccgccc                                                           >  1:3670181/1‑93 (MQ=255)

                             gAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGCCCGAcc                                                           >  2:3974384/1‑93 (MQ=255)

                             gAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGCCCGAcc                                                           >  2:1580354/1‑93 (MQ=255)

                             gAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGCCCGAcc                                                           <  2:1377978/93‑1 (MQ=255)

                             gAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGCCCGAcc                                                           <  1:780821/93‑1 (MQ=255)

                             gAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGCCCGAcc                                                           >  1:732045/1‑93 (MQ=255)

                              aaaCGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGCCCGACCt                                                          >  1:2312828/1‑93 (MQ=255)

                                aCGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGc                                                        <  1:5963102/93‑1 (MQ=255)

                                  gTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCca                                                      >  2:2297933/1‑93 (MQ=255)

                                   tCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGGCCGACCTGCcac                                                     >  2:5066294/1‑93 (MQ=255)

                                     gggCAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGCCCGCCCTGCCACaa                                                   >  1:3406944/1‑93 (MQ=255)

                                       gCAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAg                                                 >  1:6076903/1‑93 (MQ=255)

                                         aaTTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGc                                               <  1:1987356/93‑1 (MQ=255)

                                         aaTTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGc                                               <  1:1642746/93‑1 (MQ=255)

                                         aaTTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGc                                               >  2:1232340/1‑93 (MQ=255)

                                          aTTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGGGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGCa                                              >  1:5099868/1‑93 (MQ=255)

                                             tCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGCACca                                           >  2:203019/1‑93 (MQ=255)

                                             tCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGCACca                                           <  2:3091390/93‑1 (MQ=255)

                                               cTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGCACcagc                                         <  1:3766354/93‑1 (MQ=255)

                                                  aaCGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGCACCAGCAGt                                      <  2:3603325/93‑1 (MQ=255)

                                                   aCGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGCACCAGCAGtt                                     >  2:3688314/1‑93 (MQ=255)

                                                   aCGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGCACCAGCAGtt                                     <  1:5152079/93‑1 (MQ=255)

                                                    cGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGCACCAGCAGTTg                                    >  2:5710461/1‑93 (MQ=255)

                                                     gTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGCACCAGCAGTTgt                                   >  1:4922129/1‑92 (MQ=255)

                                                     gTAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGCACCAGCAGTTGc                                   <  2:5683421/93‑1 (MQ=255)

                                                      tAGTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGCACCAGCAGTTGCt                                  >  1:3031007/1‑93 (MQ=255)

                                                        gTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGCACCAGCAGTTGCTTc                                <  1:6221060/93‑1 (MQ=255)

                                                        gTGAGTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGCACCAGCAGTTGCTTc                                <  1:4709260/93‑1 (MQ=255)

                                                            gTATTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGCACCAGCAGTTGCTTCGGCg                            <  1:2016013/93‑1 (MQ=255)

                                                            gTATGGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGCACCAGCAGTTGCTTCGGCg                            <  1:686176/93‑1 (MQ=255)

                                                              aTTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGCCCGCCCTGCCACAAAGGCACCAGCAGTTGCTTCGGCGac                          <  2:1477026/93‑1 (MQ=255)

                                                              aTTGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGCACCAGCAGTTGCTTCGGCGac                          >  1:472609/1‑93 (MQ=255)

                                                                tGCCCCGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGCACCAGCAGTTGCTTCGGCGacac                        >  2:2698192/1‑93 (MQ=255)

                                                                   cccGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGCACCAGCAGTTGCTTCGGCGACACCGc                     <  2:168288/93‑1 (MQ=255)

                                                                    ccGGACTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGCACCAGCAGTTGCTTCGGCGACACCGCt                    >  2:660995/1‑93 (MQ=255)

                                                                      ggACTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGCACCAGCAGTTGCTTCGGCGACACCGCTCa                  >  1:3173779/1‑93 (MQ=255)

                                                                        aCTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGCACCAGCAGTTGCTTCGGCGACACCGCTCAcc                <  2:4728466/93‑1 (MQ=255)

                                                                        aCTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGCACCAGCAGTTGCTTCGGCGACACCGCTCAcc                <  1:3873127/93‑1 (MQ=255)

                                                                        aCTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGCACCAGCAGTTGCTTCGGCGACACCGCTCAcc                >  2:1314341/1‑93 (MQ=255)

                                                                         cTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGCACCAGCAGTTGCTTCGGCGACACCGCTCACCa               >  1:562639/1‑93 (MQ=255)

                                                                         cTGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGCACCAGCAGTTGCTTCGGCGACACCGCTCACCa               >  1:6237097/1‑93 (MQ=255)

                                                                          tGCGACAACGACACTCTACTGGTGCTGGCGAATCCCGTCGGCCCGACCTGCCACAAAGGCACCAGCAGTTGCTTCGGCGACACCGCTCACCAg              <  1:454545/93‑1 (MQ=255)

                                                                          tGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGCACCAGCAGTTGCTTCGGCGACACCGCTCACCAg              >  2:2932856/1‑93 (MQ=255)

                                                                          tGCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGCACCAGCAGTTGCTTCGGCGACACCGCTCACCAg              <  2:5927087/93‑1 (MQ=255)

                                                                          ggCGACAACGCCCCGCTCCTGGTGCGGGCGAACCCAACCGGCCCGACCTGCCACAAAGGCACCAGCAGTTGCTTCGGCGACACCGCTCACCAg              <  1:1424135/92‑1 (MQ=255)

                                                                          ggCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGCACCAGCAGTTGCTTCGGCGACACCGCTCACCAg              <  1:3108829/92‑1 (MQ=255)

                                                                           gCGACAACGACCCGCTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGCACCAGCAGTTGCTTCGGCGACACCGCTCACCAGt             <  2:5226616/93‑1 (MQ=255)

                                                                           gCGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGCACCAGCAGTTGCTTCGGCGACACCGCTCACCAGt             >  2:4859011/1‑93 (MQ=255)

                                                                            cGACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGCACCAGCAGTTGCTTCGGCGACACCGCTCACCAGTg            >  2:3906157/1‑93 (MQ=255)

                                                                             taCAACGACACGCTCGTGGTGCGGGCGAACCCCATCGGCCGGACCTGCCACAAAGGCACCAGCAGTTGCTTCGGCGACACCGCTCACCTGTgg           <  1:2149004/92‑1 (MQ=255)

                                                                             gACAACGACGCGCTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGCACCAGCAGTTGCTTCGGCGACACCGCTCACCAGTgg           <  1:2095079/93‑1 (MQ=255)

                                                                             gACAACGACACGCTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGCACCAGCAGTTGCTTCGGCGACACCGCTCACCAGTgg           >  1:4243221/1‑93 (MQ=255)

                                                                              aCAACGACACGCTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGCACCAGCAGTTGCTTCGGCGACACCGCTCACCAGTGGc          >  1:4203852/1‑93 (MQ=255)

                                                                              aCAACGACACGCTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGCACCAGCAGTTGCTTCGGCGACACCGCTCACCAGTGGc          >  1:4836935/1‑93 (MQ=255)

                                                                               cAACGACACGCTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGCACCAGCAGTTGCTTCGGCGACACCGCTCACCAGTGGCt         <  1:4771728/93‑1 (MQ=255)

                                                                                aaCGACACGCTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGCACCAGCAGTTGCTTCGGCGACACCGCTCACCAGTGGCTg        <  2:1895145/93‑1 (MQ=255)

                                                                                  cGACACGCTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGCACCAGCAGTTGCTTTGGCGACACCGCTCACCAGTGGCTGtt      <  1:2404292/93‑1 (MQ=255)

                                                                                  cGACACGCTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGCACCAGCAGTTGCTTCGGCGACACCGCTCACCAGTGGCTGtt      >  2:3454823/1‑93 (MQ=255)

                                                                                  cGACACGCTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGCACCAGCAGTTGCTTCGGCGACACCGCTCACCAGTGGCTGtt      <  2:3344434/93‑1 (MQ=255)

                                                                                   gACACGCTACTGGTGCTGGAGAATCCCATCGGCCCGACCTGCCACAAATGCACCAGCAGTTGCTTCGGCGACACCGCTCACCAGTGGCtcgtc     >  2:4783352/1‑89 (MQ=255)

                                                                                    acacGCTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGCACCAGCAGTTGCTTCGGCGACACCGCTCACCAGTGGCTGTTcc    >  2:4124638/1‑93 (MQ=255)

GCACTAAACAGCGACTGTGGACCAAAGGCGAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGTTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGCACCAGCAGTTGCTTCGGCGACACCGCTCACCAGTGGCTGTTCCTG  >  NC_000913/2094802‑2094980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`