BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,154,032	T→C	Y250H (TAT→CAT)	yegN →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,154,032	0	T	C	99.6%	897.6 / NA	260	Y250H (TAT→CAT)	yegN	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (133/126); total (134/126)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 266 total aligned reads displayed.

GCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGG  >  NC_000913/2153942‑2154121

gcTAACGTTAACTCGGCAAAAGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCAt                                                                                         <  2:5421727/93‑1 (MQ=255)

gcTAACGTTAACTCGGCAAAAGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCAt                                                                                         <  2:1451041/93‑1 (MQ=255)

  tAACGTTAACTCGGCAAAAGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGCACGACCAGATGCAATCCGCCGAAGAGCATCg                                                                                       >  1:4955354/1‑93 (MQ=255)

  tAACGTTAACTCGGCAAAAGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCg                                                                                       >  1:1287303/1‑93 (MQ=255)

  tAACGTTAACTCGGCAAAAGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCg                                                                                       >  1:4787314/1‑93 (MQ=255)

  tAACGTTAACTCGGCAAAAGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCg                                                                                       >  1:1998539/1‑93 (MQ=255)

    aCGTTAACTCGGCAAAAGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGcc                                                                                     <  2:2655849/93‑1 (MQ=255)

    aCGTTAACTCGGCAAAAGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGcc                                                                                     >  2:350911/1‑93 (MQ=255)

     cGTTAACTCGGCAAAAGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCa                                                                                    <  2:5015440/93‑1 (MQ=255)

      gTTAACTCGGCAAAAGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAg                                                                                   >  1:448699/1‑93 (MQ=255)

      gTTAACTCGGCAAAAGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAg                                                                                   <  1:3018416/93‑1 (MQ=255)

       ttAACTCGGCAAAAGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGc                                                                                  >  2:1745378/1‑93 (MQ=255)

         aaCTCGGCAAAAGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTa                                                                                >  1:6349306/1‑93 (MQ=255)

         aaCTCGGCAAAAGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTa                                                                                >  2:4068754/1‑93 (MQ=255)

          aCTCGGCAAAAGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTaa                                                                               >  2:4537867/1‑93 (MQ=255)

             cGGCAAAAGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAatca                                                                            >  1:171487/1‑93 (MQ=255)

              ggCAAAAGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAatcat                                                                           <  1:2610281/93‑1 (MQ=255)

                  aaaGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGcc                                                                       <  1:2681250/93‑1 (MQ=255)

                   aaGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCt                                                                      <  2:1540596/93‑1 (MQ=255)

                    aGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTa                                                                     >  2:5374229/1‑93 (MQ=255)

                     ggTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTAc                                                                    <  2:2573624/93‑1 (MQ=255)

                        aGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAg                                                                 <  2:4112766/93‑1 (MQ=255)

                        aGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAg                                                                 >  1:5607763/1‑93 (MQ=255)

                          ccTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGaa                                                               <  1:1485805/93‑1 (MQ=255)

                             cGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACgg                                                            >  1:530351/1‑93 (MQ=255)

                             cGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACgg                                                            <  2:5688697/93‑1 (MQ=255)

                              gACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGgc                                                           >  1:506129/1‑93 (MQ=255)

                               aCGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGgcg                                                          >  1:1785538/1‑93 (MQ=255)

                               aCGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCAGCCGAAGAGCATCGTCAGCTAATCATCGCCTACCAGAgcggcg                                                          >  2:2316520/1‑93 (MQ=255)

                                cGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGgcgc                                                         >  1:229418/1‑93 (MQ=255)

                                 ggCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGgggcg                                                        >  1:4198009/1‑93 (MQ=255)

                                  gCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGgcgcgc                                                       >  2:1689803/1‑93 (MQ=255)

                                   cccTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGcc                                                      <  2:6103509/93‑1 (MQ=255)

                                    ccTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCa                                                     >  1:3536108/1‑93 (MQ=255)

                                     cTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCaa                                                    >  2:5967026/1‑93 (MQ=255)

                                     cTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCaa                                                    >  1:1633823/1‑93 (MQ=255)

                                     cTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCaa                                                    >  1:2087749/1‑93 (MQ=255)

                                      ttCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAAt                                                   >  1:6069105/1‑93 (MQ=255)

                                      ttCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAAt                                                   <  2:3176874/93‑1 (MQ=255)

                                      ttCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAAt                                                   >  1:5449527/1‑93 (MQ=255)

                                       tCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAAtt                                                  <  1:4862014/93‑1 (MQ=255)

                                        cccGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTc                                                 <  1:4351487/93‑1 (MQ=255)

                                        cccGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTc                                                 <  2:5915457/93‑1 (MQ=255)

                                         ccGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCg                                                >  2:2060475/1‑93 (MQ=255)

                                          cGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGt                                               >  2:2985693/1‑93 (MQ=255)

                                            tGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCt                                             >  1:1201264/1‑93 (MQ=255)

                                             gCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTg                                            <  2:3740120/93‑1 (MQ=255)

                                             gCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTg                                            <  2:2190600/93‑1 (MQ=255)

                                                gTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGc                                         >  2:691576/1‑93 (MQ=255)

                                                gTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGc                                         >  1:2194516/1‑93 (MQ=255)

                                                  cACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGa                                       <  1:5730805/93‑1 (MQ=255)

                                                  cACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGa                                       <  2:2880099/93‑1 (MQ=255)

                                                   cctcTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGAt                                      <  2:4801172/90‑1 (MQ=255)

                                                    cGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATg                                     >  1:5235665/1‑93 (MQ=255)

                                                     gCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGt                                    <  1:5643199/93‑1 (MQ=255)

                                                      cTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTc                                   <  1:3507289/93‑1 (MQ=255)

                                                       tttCCGCGAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCg                                  >  2:3377754/1‑93 (MQ=255)

                                                            gcgAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACt                             >  1:2481684/1‑93 (MQ=255)

                                                              gAACGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGt                           <  1:5147163/93‑1 (MQ=255)

                                                               aaCGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTa                          >  2:329161/1‑93 (MQ=255)

                                                                aCGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTag                         >  2:1253112/1‑93 (MQ=255)

                                                                aCGACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTag                         >  2:822423/1‑93 (MQ=255)

                                                                  gACCAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTagag                       <  2:3636021/93‑1 (MQ=255)

                                                                     cAGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCaa                    >  2:2872462/1‑93 (MQ=255)

                                                                      aGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAg                   <  2:4252544/93‑1 (MQ=255)

                                                                      aGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAg                   <  2:5215412/93‑1 (MQ=255)

                                                                      aGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAg                   <  2:2734193/93‑1 (MQ=255)

                                                                      aGATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAg                   >  1:1013172/1‑93 (MQ=255)

                                                                       gATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAgg                  <  2:5836158/93‑1 (MQ=255)

                                                                       gATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAgg                  >  2:1917942/1‑93 (MQ=255)

                                                                       gATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAgg                  <  1:1135507/93‑1 (MQ=255)

                                                                       gATGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAgg                  <  1:873576/93‑1 (MQ=255)

                                                                        aTGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGt                 <  1:3802851/93‑1 (MQ=255)

                                                                        aTGCAACCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGt                 <  1:4376405/93‑1 (MQ=255)

                                                                         tGCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTTGCAACTGTAGAGCAAGGTg                <  1:6234017/93‑1 (MQ=255)

                                                                          gCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGc               <  1:5925559/93‑1 (MQ=255)

                                                                          gCAATCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGc               <  2:1769162/93‑1 (MQ=255)

                                                                            aaTCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAg             >  2:5472964/1‑93 (MQ=255)

                                                                            aaTCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAg             >  2:4402733/1‑93 (MQ=255)

                                                                             aTCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGa            >  1:3655838/1‑93 (MQ=255)

                                                                             aTCCGCCGAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGa            >  1:2440592/1‑93 (MQ=255)

                                                                                 gccgAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAAc        <  2:6407053/93‑1 (MQ=255)

                                                                                 gccgAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAAc        >  1:1469279/1‑93 (MQ=255)

                                                                                 gccgAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGCGCAGAAAAc        >  1:2930050/1‑93 (MQ=255)

                                                                                  ccgAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACa       >  2:3211405/1‑93 (MQ=255)

                                                                                  ccgAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACa       <  1:1227391/93‑1 (MQ=255)

                                                                                    gAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCTCAACTGTAGAGCAAGGTGCAGAAAACAGc     >  1:933072/1‑93 (MQ=255)

                                                                                    gAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGc     <  2:3907320/93‑1 (MQ=255)

                                                                                    gAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGc     <  2:23770/93‑1 (MQ=255)

                                                                                    gAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGc     <  1:673549/93‑1 (MQ=255)

                                                                                    gAAGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGc     <  1:2997829/93‑1 (MQ=255)

                                                                                     aaGAGCATCGCCATCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCt    <  1:4695203/93‑1 (MQ=255)

                                                                                     aaGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCt    <  2:2113367/93‑1 (MQ=255)

                                                                                     aaGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCt    <  2:6336534/93‑1 (MQ=255)

                                                                                     aaGAGCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCt    >  1:4032776/1‑93 (MQ=255)

                                                                                      agagCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTg   <  1:3119908/93‑1 (MQ=255)

                                                                                      agagCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTg   >  2:910363/1‑93 (MQ=255)

                                                                                       gagCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTgg  >  2:3105050/1‑93 (MQ=255)

                                                                                       gagCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTgg  <  2:2780386/93‑1 (MQ=255)

                                                                                       gagCATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTgg  >  1:4079623/1‑93 (MQ=255)

GCTAACGTTAACTCGGCAAAAGGTAGCCTCGACGGCCCTTCCCGTGCGGTCACGCTTTCCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGG  >  NC_000913/2153942‑2154121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`