BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,210,202	A→G	D5D (GAT→GAC)	yehS ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,210,202	0	A	G	99.2%	843.3 / ‑3.3	242	D5D (GAT→GAC)	yehS	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (108/132); total (109/133)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01

Only 100 of 244 total aligned reads displayed.

TTCCGCGGTGGCTTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATATCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCCTCTTTTAAGCTTT  >  NC_000913/2210112‑2210291

ttCCGCGGTGGCTTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTc                                                                                         >  1:3405968/1‑93 (MQ=255)

ttCCGCGGTGGCTTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTc                                                                                         >  2:579616/1‑93 (MQ=255)

ttCCGCGGTGGCTTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTc                                                                                         <  1:1398068/93‑1 (MQ=255)

  ccGCGGTGGCTTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGt                                                                                       <  2:427062/93‑1 (MQ=255)

    gcgGTGGCTTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTa                                                                                     >  1:4588045/1‑93 (MQ=255)

     cgGTGGCTTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTAc                                                                                    <  2:2613990/93‑1 (MQ=255)

      ggTGGCTTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACt                                                                                   <  1:5547895/93‑1 (MQ=255)

      ggTGGCTTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACt                                                                                   <  1:5209646/93‑1 (MQ=255)

       gTGGCTTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACtt                                                                                  <  2:6354595/93‑1 (MQ=255)

        tGGCTTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTa                                                                                 <  1:1013856/93‑1 (MQ=255)

         ggCTTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAg                                                                                <  2:2690976/93‑1 (MQ=255)

          gCTTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGc                                                                               <  1:1812778/93‑1 (MQ=255)

           cTTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCa                                                                              <  1:6348842/93‑1 (MQ=255)

           cTTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCa                                                                              <  2:4640335/93‑1 (MQ=255)

              cGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGa                                                                           <  2:1801598/93‑1 (MQ=255)

              cGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGa                                                                           <  1:658954/93‑1 (MQ=255)

                agaTTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAgg                                                                         >  1:6178072/3‑93 (MQ=255)

                  aTTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGcc                                                                       <  2:6304361/93‑1 (MQ=255)

                    tACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCtt                                                                     <  1:5111581/93‑1 (MQ=255)

                    tACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCtt                                                                     <  2:855672/93‑1 (MQ=255)

                     tcccAGCGCCAGAATCCGCCCCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGATGCGCAGAATGTCGTTACTTAGCATGAGGCCTTc                                                                    <  1:2972410/92‑1 (MQ=255)

                     aCCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTc                                                                    <  1:1753833/93‑1 (MQ=255)

                     aCCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTc                                                                    >  2:2766417/1‑93 (MQ=255)

                      cccAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCa                                                                   <  2:4069272/93‑1 (MQ=255)

                       ccAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAg                                                                  <  2:5782896/93‑1 (MQ=255)

                       ccAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAg                                                                  >  2:1955229/1‑93 (MQ=255)

                        cAGCGCCAGAATACGCGCCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAgg                                                                 <  2:4774396/93‑1 (MQ=255)

                        cAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAgg                                                                 >  2:341759/1‑93 (MQ=255)

                         aGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGgt                                                                <  1:828219/93‑1 (MQ=255)

                         aGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGgt                                                                >  2:5841139/1‑93 (MQ=255)

                          gcgcCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGgtg                                                               <  1:4934430/93‑1 (MQ=255)

                            gcCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGtt                                                             >  2:386935/1‑93 (MQ=255)

                            gcCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGtt                                                             <  2:6438533/93‑1 (MQ=255)

                                gAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTtgatg                                                         >  1:4613512/1‑93 (MQ=255)

                                gAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTtgatg                                                         >  1:327379/1‑93 (MQ=255)

                                 aaTACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTtgatga                                                        >  1:5731134/1‑93 (MQ=255)

                                 aaTACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTtgatga                                                        >  1:3145418/1‑93 (MQ=255)

                                   tACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAgg                                                      <  2:4574898/93‑1 (MQ=255)

                                     cGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCa                                                    >  2:1717210/1‑93 (MQ=255)

                                        aCCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCaaaa                                                 >  2:2317447/1‑93 (MQ=255)

                                         ccAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCaaaaa                                                >  2:5160658/1‑93 (MQ=255)

                                         ccAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCaaaaa                                                <  1:3952121/93‑1 (MQ=255)

                                           aGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGc                                              <  2:600474/93‑1 (MQ=255)

                                                 ttattattGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCAtttt                                        >  1:5458552/1‑93 (MQ=255)

                                                 ttattattGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCAtttt                                        <  1:2849147/93‑1 (MQ=255)

                                                 ttattattGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCAtttt                                        <  2:424679/93‑1 (MQ=255)

                                                  tattattGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTa                                       >  1:952161/1‑93 (MQ=255)

                                                  tatgattGTCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTGGATGAGGCAAAAAGCCATTTTa                                       <  2:1591127/93‑1 (MQ=255)

                                                    ttattGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGc                                     <  2:3201272/93‑1 (MQ=255)

                                                     tattGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCa                                    <  1:702417/93‑1 (MQ=255)

                                                     tattGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCa                                    >  1:1438913/1‑93 (MQ=255)

                                                     tattGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCa                                    >  1:4899776/1‑93 (MQ=255)

                                                      attGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAg                                   >  2:2981782/1‑93 (MQ=255)

                                                       ttGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGt                                  <  2:4890533/93‑1 (MQ=255)

                                                       ttGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGt                                  >  2:2155328/1‑93 (MQ=255)

                                                        tGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTc                                 >  2:789745/1‑93 (MQ=255)

                                                        tGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTc                                 >  2:4416778/1‑93 (MQ=255)

                                                        tGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTc                                 <  1:25999/93‑1 (MQ=255)

                                                        tGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTc                                 >  2:5544776/1‑93 (MQ=255)

                                                         ggCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCt                                <  2:6346557/93‑1 (MQ=255)

                                                          gCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCtt                               <  2:471879/93‑1 (MQ=255)

                                                          gCTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCtt                               >  2:5349977/1‑93 (MQ=255)

                                                           cTTTCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCttt                              <  1:2808866/93‑1 (MQ=255)

                                                            tttCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCtttt                             >  2:313675/1‑93 (MQ=255)

                                                             ttCAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTa                            <  1:3806483/93‑1 (MQ=255)

                                                               cAAAATGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACa                          <  2:2389486/93‑1 (MQ=255)

                                                                 aaaTGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAgg                        <  2:3596765/93‑1 (MQ=255)

                                                                 aaaTGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAgg                        <  1:2124325/93‑1 (MQ=255)

                                                                 aaaTGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAgg                        >  1:6282985/1‑93 (MQ=255)

                                                                  aaTGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGc                       <  1:2076504/93‑1 (MQ=255)

                                                                  aaTGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGc                       <  1:5093167/93‑1 (MQ=255)

                                                                   aTGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGcc                      >  2:2553882/1‑93 (MQ=255)

                                                                   aTGTAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGcc                      <  1:2301878/93‑1 (MQ=255)

                                                                      tAGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAAt                   >  1:1330623/1‑93 (MQ=255)

                                                                       aGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATc                  <  1:2464697/93‑1 (MQ=255)

                                                                       aGCGCACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATc                  <  1:5996447/93‑1 (MQ=255)

                                                                        gcgcACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCg                 >  2:5617951/1‑93 (MQ=255)

                                                                        gcgcACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCg                 >  2:3479986/1‑93 (MQ=255)

                                                                          gcACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGcc               <  1:2002938/93‑1 (MQ=255)

                                                                          gcACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGcc               <  1:4087737/93‑1 (MQ=255)

                                                                           cACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCct              >  2:2285547/1‑93 (MQ=255)

                                                                           cACGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCct              <  1:2210864/93‑1 (MQ=255)

                                                                            aCGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCctc             <  1:994656/93‑1 (MQ=255)

                                                                             cGCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCctct            <  2:503999/93‑1 (MQ=255)

                                                                              gCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCCTCtt           <  1:332746/93‑1 (MQ=255)

                                                                              gCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCCTCtt           <  2:3921236/93‑1 (MQ=255)

                                                                              gCTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCCTCtt           <  1:5770055/93‑1 (MQ=255)

                                                                               cTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCCTCttt          >  2:6136837/1‑93 (MQ=255)

                                                                               cTGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCCTCttt          >  2:1227817/1‑93 (MQ=255)

                                                                                tGCGCAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCCTCtttt         >  2:2111051/1‑93 (MQ=255)

                                                                                   gcAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCCTCTTTTAAg      >  1:1055229/1‑93 (MQ=255)

                                                                                    cAGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCCTCTTTTAAGc     >  2:118951/1‑93 (MQ=255)

                                                                                     aGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCCTCTTTTAAGCt    <  2:6213378/93‑1 (MQ=255)

                                                                                     aGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCCTCTTTTAAGCt    <  1:4395034/93‑1 (MQ=255)

                                                                                     aGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCCTCTTTTAAGCt    <  2:2034660/93‑1 (MQ=255)

                                                                                     aGAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCCTCTTTTAAGCt    <  2:6448628/93‑1 (MQ=255)

                                                                                      gAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCCTCTTTTAAGCtt   >  2:4517146/1‑93 (MQ=255)

                                                                                      gAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCCTCTTTTAAGCtt   <  1:3551554/93‑1 (MQ=255)

                                                                                      gAATGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCCTCTTTTAAGCtt   >  1:3736187/1‑93 (MQ=255)

                                                                                       aaTGTCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCCTCTTTTAAGCttt  <  1:2920172/93‑1 (MQ=255)

TTCCGCGGTGGCTTCGACATTACCCAGCGCCAGAATACGCACCAGGTCATTATTATTGGCTTTCAAAATGTAGCGCACGCTGCGCAGAATATCGTTACTTAGCATGAGGCCTTCAGGTGTTGATGAGGCAAAAAGCCATTTTAGCAGTCTTTTACAGGCCAATCGCCTCTTTTAAGCTTT  >  NC_000913/2210112‑2210291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`