BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,716,362	T→C	K63E (AAG→GAG)	yfiF ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,716,362	0	T	C	99.5%	695.5 / ‑4.5	201	K63E (AAG→GAG)	yfiF	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (116/84); total (117/84)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01

Only 100 of 208 total aligned reads displayed.

TTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCC‑GGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTC  >  NC_000913/2716272‑2716450

ttttACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCC‑GGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTCGc                                                                                        >  1:3255988/1‑93 (MQ=255)

    aCCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCC‑GGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTCGCGGTc                                                                                    >  1:2627834/1‑93 (MQ=255)

    aCCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCC‑GGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTCGCGGTc                                                                                    <  2:4870978/93‑1 (MQ=255)

    aCCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCC‑GGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTCGCGGTc                                                                                    >  1:5027958/1‑93 (MQ=255)

     ccACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCC‑GGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCa                                                                                   >  1:4454572/1‑93 (MQ=255)

     ccACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCC‑GGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCa                                                                                   >  2:379195/1‑93 (MQ=255)

      cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCC‑GGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCAc                                                                                  >  2:4722645/1‑93 (MQ=255)

      cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCC‑GGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCAc                                                                                  >  1:4886664/1‑93 (MQ=255)

      cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCC‑GGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCAc                                                                                  >  1:5406445/1‑93 (MQ=255)

       aCTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCC‑GGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACg                                                                                 >  2:5135121/1‑93 (MQ=255)

        cTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCC‑GGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACgg                                                                                >  2:6236665/1‑93 (MQ=255)

             gCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCC‑GGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTg                                                                           >  1:6270071/1‑93 (MQ=255)

              ccACCGTGATCGGCCTTTTCCGGCGTCTCATCACCC‑GGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGa                                                                          >  2:1252178/1‑93 (MQ=255)

                aCCGTGATCGGCCTTTTCCGGCGTCTCATCACCC‑GGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGAct                                                                        >  1:1293037/1‑93 (MQ=255)

                aCCGTGATCGGCCTTTTCCGGCGTCTCATCACCC‑GGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGAct                                                                        >  2:1891230/1‑93 (MQ=255)

                 cccggATCCGCCTTTTCCCGGCCCCCACCCCCCGGGCGGGGGGGAACCCGGGGGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGAct                                                                        <  2:4735905/89‑1 (MQ=255)

                 ccGTGATCGGCCTTTTCCGGCGTCTCATCACCC‑GGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGActc                                                                       >  2:2686754/1‑93 (MQ=255)

                   gTGGTCGCCTTTTTCCGGCGTCTCATCCCCC‑GGCGCGCGGGAACCCGGGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTg                                                                     <  2:2564435/93‑1 (MQ=255)

                   gTGATCGGCCTTTTCCGGCGTCTCATCCCCC‑GGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTg                                                                     <  2:6321372/93‑1 (MQ=255)

                   gTGATCGGCCTTTTCCGGCGTCTCATCACCC‑GGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTg                                                                     >  1:4830147/1‑93 (MQ=255)

                   gTGATCGGCCTTTTCCGGCGTCTCATCACCC‑GGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTg                                                                     >  1:5984349/1‑93 (MQ=255)

                     gATCGGCCTTTTCCGGCGTCTCATCCCCC‑GGCGCGCGGAAAACCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGtt                                                                   <  1:6146513/93‑1 (MQ=255)

                     aaTCGCCCTTTTCCGGCGTCTCATCACCC‑GGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGtt                                                                   <  1:2070775/92‑1 (MQ=255)

                      aTCGGCCTTTTCCGGCGTCTCATCACCC‑GGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGttt                                                                  >  2:859753/1‑93 (MQ=255)

                      aTCGGCCTTTTCCGGCGTCTCATCACCC‑GGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGttt                                                                  <  2:4361880/93‑1 (MQ=255)

                       tCGGCCTTTTCCGGCGTCTCATCACCC‑GGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTg                                                                 <  1:1054794/93‑1 (MQ=255)

                       tCGGCCTTTTCCGGCGTCTCATCACCC‑GGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTg                                                                 >  2:4665371/1‑93 (MQ=255)

                         ggCCTTTCCCGGCGTCTCATCGCCC‑GGCGCGCGGGAACCCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTc                                                               <  1:4286288/93‑1 (MQ=255)

                          gCCTTTTCCGGCGTCTCATCACCC‑GGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCa                                                              >  1:406145/1‑93 (MQ=255)

                          gCCTTTTCCGGCGTCTCATCACCC‑GGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCa                                                              >  1:2146631/1‑93 (MQ=255)

                           ccTTTTCCGGCGTCTCATCACCC‑GGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCAt                                                             >  1:5788199/1‑93 (MQ=255)

                           ccTTTTCCGCCTTCGCCCCCCCC‑GGCGCCGGGAAAACGGGGCCCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCAt                                                             <  1:5738124/93‑1 (MQ=255)

                            cTTTTCCGGCGTCTCATCACCC‑GGCGCGCGGGAAGCCGTGCGCCACGGCGAATCTTCCCACTCGCGTTCACGGGGCTGACTCTGTTTGTCATc                                                            <  1:1010123/93‑1 (MQ=255)

                            cTTTTCCGGCGTCTCATCACCC‑GGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATc                                                            >  2:5942601/1‑93 (MQ=255)

                            cTTTTCCGGCGTCTCATCACCC‑GGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATc                                                            <  2:1090176/93‑1 (MQ=255)

                            cTTTCCGGGCGTCTCACCCCCC‑GGCGCGGGGGAACCCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATc                                                            <  2:2535484/93‑1 (MQ=255)

                             ttttCCGGCCTCCCCCCCCCG‑GGCGCGCGGGAACCCGTGCCCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATcg                                                           <  2:3337559/93‑1 (MQ=255)

                             tttcCCGGCGTCTCACCCCCC‑GGCGCGCGGGAACCCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATcg                                                           <  1:159179/93‑1 (MQ=255)

                              tttCCGGCGTCTCATCACCC‑GGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTATCATcgc                                                          >  2:944672/1‑93 (MQ=255)

                              ttcccgccgTCCCACCCCCG‑GGCGCGCGGAAACCCGTGCCCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATcgc                                                          <  1:3809590/90‑1 (MQ=255)

                                 ccGGCGTCTCATCACCC‑GGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCgcggg                                                       >  1:6409280/1‑93 (MQ=255)

                                 ccGGCGTCTCATCACCC‑GGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCgcggg                                                       >  2:3550351/1‑93 (MQ=255)

                                      gTCTCATCGCCC‑GGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCgggcgggg                                                  <  2:3208146/93‑1 (MQ=255)

                                      gTCTCATCACCC‑GGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCgggcgggg                                                  >  1:6227350/1‑93 (MQ=255)

                                       tctcATCACCC‑GGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCgggcggggc                                                 <  1:677194/93‑1 (MQ=255)

                                       tctcACCCCCG‑GGCGCGCGGGAACCCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCgggcggggc                                                 <  1:3500764/93‑1 (MQ=255)

                                        ctcATCACCC‑GGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCgggcggggcg                                                >  2:2070858/1‑93 (MQ=255)

                                           atcaCCC‑GGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACg                                             <  1:5222611/93‑1 (MQ=255)

                                           atcaCCC‑GGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACg                                             >  2:2502764/1‑93 (MQ=255)

                                           atcaCCC‑GGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACg                                             >  2:1937781/1‑93 (MQ=255)

                                            tcaCCC‑GGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCTACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACgg                                            >  2:4536194/1‑93 (MQ=255)

                                            tcaCCC‑GGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACgg                                            <  2:1011121/93‑1 (MQ=255)

                                            tcaCCC‑GGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACgg                                            >  2:1613390/1‑93 (MQ=255)

                                            tcaCCC‑GGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACgg                                            >  2:2008122/1‑93 (MQ=255)

                                            ccaCCC‑GGCGCGCGGGAACCCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACgg                                            <  1:2869534/92‑1 (MQ=255)

                                             ccccc‑GGCGCGGGGGAACCCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGc                                           <  1:5800526/91‑1 (MQ=255)

                                             caCCC‑GGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGc                                           >  1:2959791/1‑93 (MQ=255)

                                                cc‑GGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCAc                                        <  2:4120791/93‑1 (MQ=255)

                                                   ggCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCg                                      >  2:2220666/1‑93 (MQ=255)

                                                          gggAACCCGTGCGCCACGGCGAATCTTCCCGCTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCAc                               <  1:709895/93‑1 (MQ=255)

                                                           ggAAACCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGGCACCGTCAGCACg                              >  1:4732493/1‑93 (MQ=255)

                                                           ggAAACCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACg                              >  2:5331040/1‑93 (MQ=255)

                                                             aaaCCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGGCGGCCACCGTCAGCACgag                            >  1:4577576/1‑93 (MQ=255)

                                                              aaCCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACgagt                           >  2:3695300/1‑92 (MQ=255)

                                                              aaCCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACgaga                           >  2:2049754/1‑93 (MQ=255)

                                                              aaCCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACgaga                           >  1:2258261/1‑93 (MQ=255)

                                                               aCCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGAt                          >  2:3983153/1‑93 (MQ=255)

                                                               aCCGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGAt                          <  2:4634628/93‑1 (MQ=255)

                                                                ccGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGAtt                         >  1:1508449/1‑93 (MQ=255)

                                                                 cGTGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGAttt                        <  1:3208305/93‑1 (MQ=255)

                                                                  gTGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGAtttt                       <  1:3935897/93‑1 (MQ=255)

                                                                   tGCGCCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTc                      <  1:4239818/93‑1 (MQ=255)

                                                                    gcgcCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTcc                     <  2:1392430/93‑1 (MQ=255)

                                                                    gcgcCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTcc                     <  1:6453985/93‑1 (MQ=255)

                                                                    gccccACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTcc                     <  2:5492271/90‑1 (MQ=255)

                                                                      gcCACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTg                   >  2:2879931/1‑93 (MQ=255)

                                                                       ccACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTgg                  <  2:6278884/93‑1 (MQ=255)

                                                                       ccACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTgg                  <  2:2764686/93‑1 (MQ=255)

                                                                       ccACGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTgg                  <  2:2540956/93‑1 (MQ=255)

                                                                       caaCGGCGAATCTTCCCACTCGCGGTCAGGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTgg                  <  2:4425002/91‑1 (MQ=255)

                                                                         aCGGCGAAGCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAc                <  1:3671905/93‑1 (MQ=255)

                                                                          cGGCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAcg               <  1:5946104/93‑1 (MQ=255)

                                                                            gCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAcgcc             <  2:3950789/93‑1 (MQ=255)

                                                                            gCGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAcgcc             >  2:6049572/1‑93 (MQ=255)

                                                                             cGAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTGTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAcgccc            <  1:4781872/93‑1 (MQ=255)

                                                                              gAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAcgcccg           >  2:3038014/1‑93 (MQ=255)

                                                                              gAATCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAcgcccg           <  1:5606509/93‑1 (MQ=255)

                                                                               aaTCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGCCAGCACGAGATTTTCCTGGAcgcccgc          >  2:152101/1‑93 (MQ=255)

                                                                               aaTCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCCCGGGCGGGGCGTCGGCCACCGTCAGCACGAGACTTTTCTGGAcgcccgc          >  1:1712499/1‑93 (MQ=255)

                                                                                aTCTTCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAcgcccgcc         >  2:5020051/1‑93 (MQ=255)

                                                                                   ttCCCACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGccccc      <  1:5528211/93‑1 (MQ=255)

                                                                                     cccACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCtt    >  1:3616745/1‑93 (MQ=255)

                                                                                     cccACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCtt    <  1:4371155/93‑1 (MQ=255)

                                                                                     cccACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCtt    >  2:5375100/1‑93 (MQ=255)

                                                                                     cccACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCtt    <  1:1871102/93‑1 (MQ=255)

                                                                                     cccACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCtt    >  2:1581915/1‑93 (MQ=255)

                                                                                      ccACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCttt   >  1:1809847/1‑93 (MQ=255)

                                                                                       cACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTc  <  2:6097631/93‑1 (MQ=255)

                                                                                       cACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTc  >  2:3161611/1‑93 (MQ=255)

                                                                                       cACTCGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTc  <  1:5612720/93‑1 (MQ=255)

TTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCC‑GGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTC  >  NC_000913/2716272‑2716450

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`