BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,865,091	T→C	T162A (ACC→GCC)	rpoS ←	sigma S factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,865,091	0	T	C	99.6%	902.2 / NA	261	T162A (ACC→GCC)	rpoS	sigma S factor
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (125/135); minor base A (1/0); total (126/135)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 271 total aligned reads displayed.

CATGGTCCAGCTTATGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCT  >  NC_000913/2865001‑2865180

  tGGTCCAGCTTATGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTgg                                                                                       >  1:5016610/1‑93 (MQ=255)

  tGGTCCAGCTTATGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTgg                                                                                       <  2:4640583/93‑1 (MQ=255)

  tGGTCCAGCTTATGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTgg                                                                                       <  2:5330495/93‑1 (MQ=255)

  tGGTCCAGCTTATGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCATTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTgg                                                                                       >  1:2990065/1‑93 (MQ=255)

   ggTCCAGCTTATGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGt                                                                                      >  2:3130093/1‑93 (MQ=255)

    gTCCAGCTTATGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGtt                                                                                     >  2:4649975/1‑93 (MQ=255)

      ccAGCTTATGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCa                                                                                   <  2:3414077/93‑1 (MQ=255)

        aGCTTATGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATa                                                                                 >  2:1652373/1‑93 (MQ=255)

        aGCTTATGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATa                                                                                 >  2:307877/1‑93 (MQ=255)

          cTTATGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAAt                                                                               <  2:5144816/93‑1 (MQ=255)

           ttATGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATc                                                                              >  2:5532370/1‑93 (MQ=255)

            tATGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCg                                                                             >  1:5843986/1‑93 (MQ=255)

             aTGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGc                                                                            >  1:3643921/1‑93 (MQ=255)

              tGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGcc                                                                           >  2:4225412/1‑93 (MQ=255)

              tGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGcc                                                                           <  2:2963983/93‑1 (MQ=255)

              tGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGcc                                                                           <  1:1568396/93‑1 (MQ=255)

               gggACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGccc                                                                          <  2:1819370/93‑1 (MQ=255)

                ggACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCg                                                                         <  2:1760991/93‑1 (MQ=255)

                ggACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGGTCATAATCGCCCg                                                                         >  1:5312177/1‑93 (MQ=255)

                     aCTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTGCGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCaa                                                                    <  2:2508125/93‑1 (MQ=255)

                     aCTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGGCCGTTCaa                                                                    >  2:5925974/1‑93 (MQ=255)

                      cTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCCAt                                                                   >  1:4022676/1‑93 (MQ=255)

                      cTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAAt                                                                   >  2:4573471/1‑93 (MQ=255)

                      cTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAAt                                                                   <  2:853295/93‑1 (MQ=255)

                       tCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATc                                                                  <  1:1015560/93‑1 (MQ=255)

                       tCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATc                                                                  <  2:785634/93‑1 (MQ=255)

                         aCGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGt                                                                >  2:2160304/1‑93 (MQ=255)

                          cGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTc                                                               <  2:1974692/93‑1 (MQ=255)

                          cGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTc                                                               >  1:1534498/1‑93 (MQ=255)

                          cGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTc                                                               <  1:1786126/93‑1 (MQ=255)

                           gTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCt                                                              <  1:1044801/93‑1 (MQ=255)

                            tGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTg                                                             <  2:2396242/93‑1 (MQ=255)

                            tGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTg                                                             <  1:2412814/93‑1 (MQ=255)

                             gCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTgg                                                            <  2:4867121/93‑1 (MQ=255)

                              cGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGc                                                           <  1:709711/93‑1 (MQ=255)

                              cGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGc                                                           <  2:4338487/93‑1 (MQ=255)

                                gTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGa                                                         >  2:3204276/1‑93 (MQ=255)

                                    gCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATcc                                                     <  1:1284966/93‑1 (MQ=255)

                                    gCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATcc                                                     >  2:5386256/1‑93 (MQ=255)

                                     cAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATcca                                                    >  2:3906341/1‑93 (MQ=255)

                                      aGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATccac                                                   <  1:4690101/93‑1 (MQ=255)

                                      aGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATccac                                                   >  2:2065523/1‑93 (MQ=255)

                                       ggTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATccacc                                                  <  2:3939851/93‑1 (MQ=255)

                                       ggTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATccacc                                                  <  1:544302/93‑1 (MQ=255)

                                         tAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAg                                                >  1:3911419/1‑93 (MQ=255)

                                          aaaCGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAgg                                               >  1:2241389/1‑93 (MQ=255)

                                           aaCGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAggg                                              >  1:5201573/1‑92 (MQ=255)

                                           aaCGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGt                                              <  2:2643557/93‑1 (MQ=255)

                                           aaCGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGt                                              <  2:2695117/93‑1 (MQ=255)

                                           aaCGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGt                                              >  2:4752822/1‑93 (MQ=255)

                                           aaCGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGt                                              >  2:1377642/1‑93 (MQ=255)

                                           aaCGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGt                                              >  1:5429913/1‑93 (MQ=255)

                                            aCGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGtt                                             >  1:4441633/1‑93 (MQ=255)

                                            aCGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAAACGTCTGGCGAATCCACCAGGtt                                             >  1:6309614/1‑93 (MQ=255)

                                             cGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCANTCGTCTGGCGAATNCACCAGGTTg                                            >  1:2210043/1‑93 (MQ=255)

                                              gTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTAGCGAATCCACCAGGTTGc                                           >  1:526017/1‑93 (MQ=255)

                                                 cAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTa                                        >  1:2289131/1‑93 (MQ=255)

                                                  aGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTAt                                       <  2:3355049/93‑1 (MQ=255)

                                                   gCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATg                                      <  1:5559754/93‑1 (MQ=255)

                                                   gCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATg                                      >  1:2655673/1‑93 (MQ=255)

                                                     tCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCCCCAGGATGCGTATgct                                    >  2:6102731/1‑91 (MQ=255)

                                                     tCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGtt                                    >  2:1564480/1‑93 (MQ=255)

                                                     tCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGtt                                    >  1:6444816/1‑93 (MQ=255)

                                                     tCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGtt                                    >  2:6471005/1‑93 (MQ=255)

                                                        tttACGATGTGAATCGGCAAACGAATAGTGCGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTgag                                 <  2:1295517/93‑1 (MQ=255)

                                                        tttACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTgag                                 <  2:2544482/93‑1 (MQ=255)

                                                        tttACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTgag                                 <  1:3279137/93‑1 (MQ=255)

                                                        tttACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGGTGCGTATGTTgag                                 >  1:4649719/1‑93 (MQ=255)

                                                          tACGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGaa                               <  1:681083/93‑1 (MQ=255)

                                                           aCGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAg                              <  1:3521232/93‑1 (MQ=255)

                                                           aCGATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAg                              >  2:426681/1‑93 (MQ=255)

                                                             gATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCg                            <  1:6415236/93‑1 (MQ=255)

                                                             gATGTGAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCg                            >  2:1061105/1‑93 (MQ=255)

                                                               tgtgAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGa                          >  2:3434034/1‑93 (MQ=255)

                                                                gtgAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGaa                         >  1:4397711/1‑93 (MQ=255)

                                                                gtgAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAAACGTCTGGCGAATCCACCAGGATGCGTACGTTGAGAAGCGGaa                         >  2:1444713/1‑93 (MQ=255)

                                                                  gAATCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGaatc                       <  1:3349299/93‑3 (MQ=255)

                                                                   aaTCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAAcc                      <  1:2344821/93‑1 (MQ=255)

                                                                   aaTCGGCAAACGAATAGTACGGCCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGCATCCACCCGTGTTCGTATGATGAGAAAGGGAAAcc                      >  1:163206/1‑93 (MQ=255)

                                                                    aTCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCa                     >  1:1914312/1‑93 (MQ=255)

                                                                     tCGGCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCAc                    <  2:618319/93‑1 (MQ=255)

                                                                       ggCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGt                  <  1:2929132/93‑1 (MQ=255)

                                                                        gCAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGtt                 >  1:2678132/1‑93 (MQ=255)

                                                                        gCAAACCAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGtt                 <  2:6360406/93‑1 (MQ=255)

                                                                         cAAACGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTc                <  1:5627464/93‑1 (MQ=255)

                                                                          aaaCGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTcc               >  2:4983697/1‑93 (MQ=255)

                                                                          aaaCGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTcc               <  1:1986545/93‑1 (MQ=255)

                                                                          aaaCGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTcc               <  2:5263443/93‑1 (MQ=255)

                                                                          aaaCGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTcc               >  2:6116699/1‑93 (MQ=255)

                                                                            aCGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCgg             <  1:1241751/93‑1 (MQ=255)

                                                                            aCGAATAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCgg             <  2:368491/93‑1 (MQ=255)

                                                                              gAATAGTACGGGCTTGTTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGt           <  1:1154765/93‑1 (MQ=255)

                                                                               aaTAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTc          >  2:4089464/1‑93 (MQ=255)

                                                                               aaTAGTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTc          <  1:2794086/93‑1 (MQ=255)

                                                                                   gTACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAAc      >  1:2117109/1‑93 (MQ=255)

                                                                                   gTACGGGCGTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAAc      >  1:6285600/1‑93 (MQ=255)

                                                                                    tACGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACt     <  1:5927949/93‑1 (MQ=255)

                                                                                      cGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTtc   <  1:5770416/93‑1 (MQ=255)

                                                                                      cGGGCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTtc   <  2:1239831/93‑1 (MQ=255)

                                                                                       gggCTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGCAAGCACGTTCCGGGGCAAACTtct  >  1:3209730/1‑93 (MQ=255)

CATGGTCCAGCTTATGGGACAACTCACGTGCGGTTCGCAGGTAAACGTTCAGCTCCTTTACGATGTGAATCGGCAAACGAATAGTACGGGTTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCT  >  NC_000913/2865001‑2865180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`