BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	272,747	T→C	L221P (CTA→CCA)	ykfC →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	272,747	0	T	C	99.6%	795.8 / NA	231	L221P (CTA→CCA)	ykfC	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (97/133); minor base A (0/1); total (97/134)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 247 total aligned reads displayed.

TGTGGAAAACCATCAAGGCGGGACATATCGATGTCGGTCTCTTTCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCTATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTACTGGA  >  NC_000913/272657‑272836

 gtgGAAAACCATCAAGGCGGGACATATCGATGTCGGTCTCTTTCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCcatt                                                                                        >  2:1196337/1‑93 (MQ=255)

  tgGAAAACCATCAAGGCGGGACATATCGATGTCGGTCTCTTTCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCcatta                                                                                       <  2:6043939/93‑1 (MQ=255)

    gAAAACCATCAAGGCGGGACATATCGATGTCGGTCTCTTTCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATc                                                                                     <  2:562640/93‑1 (MQ=255)

    gAAAACCATCAAGGCGGGACATATCAATGTCGGTCTCTTTCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATc                                                                                     >  1:3657764/1‑93 (MQ=255)

     gaaaCCATCAAGGCGGGACATATCGATGTCGGTCTCTTTCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCg                                                                                    <  2:5352525/92‑1 (MQ=255)

         ccATCAAGGCGGGACATATCGATGTCGGTCTCTTTCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAAca                                                                                <  1:6089879/93‑1 (MQ=255)

         ccATCAAGGCGGGACATATCGATGTCGGTCTCTTTCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAAca                                                                                >  2:4692082/1‑93 (MQ=255)

         ccATCAAGGCGGGACATATCGATGTCGGTCTCTTTCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAAca                                                                                <  1:5622008/93‑1 (MQ=255)

         ccATCAAGGCGGGACATATCGATGTCGGTCTCTTTCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAAca                                                                                >  2:6234364/1‑93 (MQ=255)

           aTCAAGGCGGGACATATCGATGTCGGTCTCTTTCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAAcatc                                                                              <  2:4297660/93‑1 (MQ=255)

            tCAAGGCGGGACATATCGATGTCGGTCTCTTTCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAAcatca                                                                             <  2:2816045/93‑1 (MQ=255)

             cAAGGCGGGACATATCGATGTCGGTCTCTTTCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAAcatcat                                                                            <  2:2787446/93‑1 (MQ=255)

                ggCGGGACATATCGATGTCGGTCTCTTTCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCt                                                                         >  2:1343551/1‑93 (MQ=255)

                ggCGGGACATATCGATGTCGGTCTCTTTCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCt                                                                         <  2:1885779/93‑1 (MQ=255)

                ggCGGGACATATCGATGTCGGTCTCTTTCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCt                                                                         >  1:4486881/1‑93 (MQ=255)

                   gggACATATCGATGTCGGTCTCTTTCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGaa                                                                      >  1:6046226/1‑93 (MQ=255)

                   gggACATATCGATGTCGGTCTCTTTCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGaa                                                                      <  2:4998243/93‑1 (MQ=255)

                    ggACATATCGATGTCGGTCTCTTTCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAAt                                                                     <  1:1880593/93‑1 (MQ=255)

                    ggACATATCGATGTCGGTCTCTTTCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAAt                                                                     >  2:3742960/1‑93 (MQ=255)

                     gACATATCGATGTCGGTCTCTTTCGGGCGGCCAGTGAAGGTGTGCCACAGGGGGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATg                                                                    <  1:5787630/93‑1 (MQ=255)

                      aCATATCGATGTCGGTCTCTTTCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGa                                                                   <  2:2390178/93‑1 (MQ=255)

                      aCATATCGATGTCGGTCTCTTTCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGa                                                                   >  1:4817894/1‑93 (MQ=255)

                      aCATATCGATGTCGGTCTCTTTCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGa                                                                   <  1:4652491/93‑1 (MQ=255)

                        atatCGATGTCGGTCTCTTTCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGt                                                                 >  2:1068298/1‑93 (MQ=255)

                        atatCGATGTCGGTCTCTTTCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGt                                                                 <  2:6342729/93‑1 (MQ=255)

                         tatCGATGTCGGTCTCTTTCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGtt                                                                <  1:5462705/93‑1 (MQ=255)

                         tatCGATGTCGGTCTCTTTCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGtt                                                                <  2:5823570/93‑1 (MQ=255)

                          atCGATGTCGGTCTCTTTCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTc                                                               <  2:1575264/93‑1 (MQ=255)

                          atCGATGTCGGTCTCTTTCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTc                                                               <  1:331578/93‑1 (MQ=255)

                            cGATGTCGGTCTCTTTCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGa                                                             <  1:2948335/93‑1 (MQ=255)

                             gATGTCGGTCTCTTTCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGAt                                                            >  1:2754522/1‑93 (MQ=255)

                             gATGTCGGTCTCTTTCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGAt                                                            <  2:2174413/93‑1 (MQ=255)

                             gATGTCGGTCTCTTTCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGAt                                                            <  2:5185771/93‑1 (MQ=255)

                              aggTCGGTCTCTTTCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATc                                                           <  2:3134251/91‑1 (MQ=255)

                              aTGTCGGTCTCTTTCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATc                                                           <  2:3062585/93‑1 (MQ=255)

                               tGTCGGTCTCTTTCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCa                                                          <  1:3539468/93‑1 (MQ=255)

                                 tCGGTCTCTTTCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAAt                                                        <  2:3577861/93‑1 (MQ=255)

                                  cGGTCTCTTTCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATa                                                       >  2:208644/1‑93 (MQ=255)

                                   ggTCTCTTTCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATAc                                                      <  2:414996/93‑1 (MQ=255)

                                   ggTCTCTTTCGGGCGCCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATAc                                                      <  1:2480000/93‑1 (MQ=255)

                                    gTCTCTTTCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATAct                                                     >  1:153077/1‑92 (MQ=255)

                                    gTCTCTTTCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATAcc                                                     >  1:2249455/1‑93 (MQ=255)

                                    gTCTCTTTCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGATCGATCAATAcc                                                     >  2:1481660/1‑93 (MQ=255)

                                     tctcTTTCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCt                                                    <  1:771578/93‑1 (MQ=255)

                                     gctctTTCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCt                                                    <  2:5709194/92‑1 (MQ=255)

                                      ttctTTCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTg                                                   <  1:691251/92‑1 (MQ=255)

                                        ctTTCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCa                                                 <  1:795259/93‑1 (MQ=255)

                                         tttCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCAt                                                <  1:1392550/93‑1 (MQ=255)

                                         tttCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCAt                                                >  1:3829782/1‑93 (MQ=255)

                                         tttCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCAt                                                <  2:5631904/93‑1 (MQ=255)

                                          ttCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATg                                               <  1:4355737/93‑1 (MQ=255)

                                           tCGGGCGGCCAGTGAAGTTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGa                                              <  2:4906264/93‑1 (MQ=255)

                                            cGGGCGGCCAGTGAAGGTGTGCCACATGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAg                                             <  2:1031652/93‑1 (MQ=255)

                                            cGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAg                                             <  2:5491186/93‑1 (MQ=255)

                                            cGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAg                                             >  2:4584236/1‑93 (MQ=255)

                                             gggCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAgc                                            >  2:2977553/1‑93 (MQ=255)

                                               gcggcCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAgcgc                                          >  2:1420466/1‑93 (MQ=255)

                                                  gcCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCAGTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTAc                                       >  2:4869130/1‑93 (MQ=255)

                                                   ccAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTAcc                                      <  1:5831809/93‑1 (MQ=255)

                                                   ccAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTAcc                                      >  1:2405069/1‑93 (MQ=255)

                                                   ccAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTAcc                                      >  1:6217266/1‑93 (MQ=255)

                                                    cAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCt                                     <  1:2592465/93‑1 (MQ=255)

                                                      gTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGa                                   <  2:2462907/93‑1 (MQ=255)

                                                       tGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAg                                  <  2:404548/93‑1 (MQ=255)

                                                        gAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGc                                 <  1:1210168/93‑1 (MQ=255)

                                                         aaGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCg                                <  1:5598687/93‑1 (MQ=255)

                                                          aGGTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCgg                               <  2:6438663/93‑1 (MQ=255)

                                                           ggTGTGCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCggg                              <  1:505289/93‑1 (MQ=255)

                                                              gtgCCACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGaaa                           <  1:1294142/93‑1 (MQ=255)

                                                                 ccACAGGGCGTTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGcc                        <  2:4383503/93‑1 (MQ=255)

                                                                 ccACAGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGcc                        <  1:5546027/93‑1 (MQ=255)

                                                                  cgcaGGGCGGTGTTATATGGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCa                       <  1:266407/91‑1 (MQ=255)

                                                                  cacaGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCa                       >  1:3147065/1‑93 (MQ=255)

                                                                   acaGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCCGCATGAGCGCTACCTGAGCGGGAAAGCCAg                      >  1:413795/1‑93 (MQ=255)

                                                                    caGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGa                     <  2:2570176/93‑1 (MQ=255)

                                                                     aGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGaa                    <  1:3480186/93‑1 (MQ=255)

                                                                     aGGGCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGaa                    <  2:1149868/93‑1 (MQ=255)

                                                                       ggCGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGaaaa                  <  2:3417758/93‑1 (MQ=255)

                                                                         gggTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGa                <  1:4075141/92‑1 (MQ=255)

                                                                         gggTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGa                <  2:3720745/92‑1 (MQ=255)

                                                                         cGGTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGa                <  2:719471/93‑1 (MQ=255)

                                                                          ggTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGAt               <  2:329515/93‑1 (MQ=255)

                                                                          ggTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGAt               >  1:1200452/1‑93 (MQ=255)

                                                                          ggTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGAt               >  1:2068476/1‑93 (MQ=255)

                                                                          ggTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGAt               <  1:4874270/93‑1 (MQ=255)

                                                                          ggTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGAt               <  2:1797044/93‑1 (MQ=255)

                                                                          ggTGTTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGAt               <  1:5415688/93‑1 (MQ=255)

                                                                           gtgtTATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATc              >  1:6427626/1‑93 (MQ=255)

                                                                           gtgtTATATCGCCGCAATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATc              <  2:5045791/93‑1 (MQ=255)

                                                                              ttATATCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCggt           >  1:6300366/1‑93 (MQ=255)

                                                                                atatCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCggtgg         >  1:442944/1‑93 (MQ=255)

                                                                                 tatCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCggtggt        <  2:3375119/93‑1 (MQ=255)

                                                                                  atCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTa       <  2:5085460/93‑1 (MQ=255)

                                                                                  atCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTa       <  1:1642802/93‑1 (MQ=255)

                                                                                   tCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTAc      >  2:1536835/1‑93 (MQ=255)

                                                                                   tCGCCGCCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTAc      <  2:224908/93‑1 (MQ=255)

                                                                                    cgccgcCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTACt     >  1:1087943/1‑93 (MQ=255)

                                                                                      ccgcCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTACTgg   <  2:6116521/93‑1 (MQ=255)

                                                                                       cgcCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTACTGGa  >  1:5071546/1‑93 (MQ=255)

                                                                                       cgcCATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTACTGGa  >  2:5017060/1‑93 (MQ=255)

TGTGGAAAACCATCAAGGCGGGACATATCGATGTCGGTCTCTTTCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCTATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTACTGGA  >  NC_000913/272657‑272836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`