BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,013,656	T→C	M159T (ATG→ACG)	ygfJ →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,013,656	0	T	C	99.5%	692.7 / ‑5.7	197	M159T (ATG→ACG)	ygfJ	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (59/137); minor base G (1/0); total (60/137)
Fisher's exact test for biased strand distribution p-value = 3.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 197 total aligned reads displayed.

TCCCCGGCCATCCGATTTTAGTGTCAAAACCATGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTATGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGT  >  NC_000913/3013566‑3013745

tCCCCGGCCATCCGATTTTAGTGTCAAAACCATGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACgg                                                                                         <  1:5836688/93‑1 (MQ=255)

 ccccGGCCATCCGATTTTAGTGTCAAAACCATTCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACggg                                                                                        <  2:6357178/93‑1 (MQ=255)

 ccccGGCCATCCGATTTTAGTGTCAAAACCATGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACggg                                                                                        <  2:3913913/93‑1 (MQ=255)

  cccGGCCATCCGATTTTAGTGTCAAAACCATGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGga                                                                                       <  1:775623/93‑1 (MQ=255)

  cccGGCCATCCCATTTTAGTGTAAAAACCATGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGga                                                                                       <  1:4106980/93‑1 (MQ=255)

   ccGGCCATCCGATTTTAGTGTCAAAACCATGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGgag                                                                                      <  2:2180859/93‑1 (MQ=255)

   ccGGCCATCCGATTTTAGTGTCAAAACCATGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGgag                                                                                      <  2:3169377/93‑1 (MQ=255)

   ccGGCCATCCGATTTTAGTGTCAAAACCATGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGgag                                                                                      <  1:875814/93‑1 (MQ=255)

   ccGGCCATCCGATTTTAGTGTCAAAACCATGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGgag                                                                                      >  2:3555288/1‑93 (MQ=255)

   ccGGCCATCCGATTTTAGTGTCAAAACCATGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGgag                                                                                      >  1:4398276/1‑93 (MQ=255)

   ccGGCCATCCGATTTTAGTGTCAAAACCATGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGgag                                                                                      <  2:4563289/93‑1 (MQ=255)

     ggCCATCCGATTTTAGTGTCAAAACCATGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGAt                                                                                    <  1:1882198/93‑1 (MQ=255)

     ggCCATCCGATTTTAGTGTCAAAACCATGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGAt                                                                                    <  1:98676/93‑1 (MQ=255)

     ggCCATCCGATTTTAGTGTCAAAACCATGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGAt                                                                                    <  2:5084569/93‑1 (MQ=255)

     ggCCATCCGATTTTAGTGTCAAAACCATGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGAt                                                                                    <  2:264234/93‑1 (MQ=255)

      gCCATCCGATTTTAGTGTCAAAACCATGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATc                                                                                   <  1:2390307/93‑1 (MQ=255)

      gCCATCCGATTTTAGTGTCAAAACCATGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATc                                                                                   <  1:3943568/93‑1 (MQ=255)

         aTCCGATTTTAGTGTCAAAACCATGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCatt                                                                                <  1:4211326/93‑1 (MQ=255)

          tCCGATTTTAGTGTCAAAACCATGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCatta                                                                               >  1:4491946/1‑93 (MQ=255)

          tCCGATTTTAGTGTCAAAACCATGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCatta                                                                               >  2:5844406/1‑93 (MQ=255)

          tCCGATTTTAGTGTCAAAACCATGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCatta                                                                               <  1:1362544/93‑1 (MQ=255)

          tCCGATTTTAGTGTCAAAACCATGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCatta                                                                               <  1:6157056/93‑1 (MQ=255)

           ccGATTTTAGTGTCAAAACCATGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGCCAGGCACTCCTTACGGGAGATCattat                                                                              >  2:2754990/1‑93 (MQ=255)

            cGATTTTAGTGTCAAAACCATGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCattatt                                                                             <  2:5592143/93‑1 (MQ=255)

            cGATTTTAGTGTCAAAACCATACCTGATGCAGGCAATCCAACGACACAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCattatt                                                                             <  2:2080151/93‑1 (MQ=255)

              aTTTTAGTGTCAAAACCATGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTcc                                                                           >  1:2173069/1‑93 (MQ=255)

                tttAGTGTCAAAACCATGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGt                                                                         <  1:3210559/93‑1 (MQ=255)

                 ttAGTGTCAAACCCATGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTc                                                                        <  2:3541800/93‑1 (MQ=255)

                 ttAGTGTCAAAACCATGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTc                                                                        <  1:5524430/93‑1 (MQ=255)

                  tAGTGTCACAACCATGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCg                                                                       >  1:6277019/1‑93 (MQ=255)

                   aGTGTCAAAACCATGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGa                                                                      <  2:3228118/93‑1 (MQ=255)

                   aGTGTCAAAACCATGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGa                                                                      <  2:3350543/93‑1 (MQ=255)

                   aGTGTCAAAACCATGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGa                                                                      <  2:41056/93‑1 (MQ=255)

                    gtgtCAAAACCATGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCat                                                                                 <  2:5480205/81‑1 (MQ=255)

                    gtgtCAAAACCATGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGaa                                                                     >  2:6157184/1‑93 (MQ=255)

                     tgtCAAAACCATGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGaaa                                                                    <  2:2363658/93‑1 (MQ=255)

                       tCAAAACCATGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATa                                                                  <  1:2693341/93‑1 (MQ=255)

                       tCAAAACCATGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATa                                                                  <  1:1562796/93‑1 (MQ=255)

                        cAAAACCATGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATAg                                                                 >  1:100234/1‑93 (MQ=255)

                        cAAAACCATGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATAg                                                                 <  2:1352098/93‑1 (MQ=255)

                          aaaCCATGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATAGaa                                                               >  2:2461495/1‑93 (MQ=255)

                            aCCATGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATAGaaaa                                                             >  2:2036067/1‑93 (MQ=255)

                            aCCATGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATAGaaaa                                                             >  2:3718930/1‑93 (MQ=255)

                            aCCATGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATAGaaaa                                                             >  2:863686/1‑93 (MQ=255)

                            aCCATGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATAGaaaa                                                             <  1:1213184/93‑1 (MQ=255)

                            aCCATGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATAGaaaa                                                             >  1:4944408/1‑93 (MQ=255)

                             ccATGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATAGAAAAt                                                            >  2:5104663/1‑93 (MQ=255)

                             ccATGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATAGAAAAt                                                            <  2:4421100/93‑1 (MQ=255)

                             ccATGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATAGAAAAt                                                            >  1:2492567/1‑93 (MQ=255)

                             ccATGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATAGAAAAt                                                            <  1:3685019/93‑1 (MQ=255)

                             ccATGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATAGAAAAt                                                            <  1:4260841/93‑1 (MQ=255)

                              cATGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATAGAAAATg                                                           <  2:3943265/93‑1 (MQ=255)

                               aTGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATAGAAAATGc                                                          <  1:2958391/93‑1 (MQ=255)

                                 gCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATAGAAAATGCAg                                                        <  1:4812772/93‑1 (MQ=255)

                                   cTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGaa                                                      >  1:1090360/1‑93 (MQ=255)

                                     gATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAaat                                                    >  1:1286383/1‑93 (MQ=255)

                                     gATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAaat                                                    >  1:3803367/1‑93 (MQ=255)

                                      aTGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAaata                                                   <  1:5748776/93‑1 (MQ=255)

                                        gCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAaataat                                                 <  1:6358145/93‑1 (MQ=255)

                                        gCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAaataat                                                 <  1:5332234/93‑1 (MQ=255)

                                         cAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAAtt                                                <  2:2751062/93‑1 (MQ=255)

                                          aGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAAttt                                               <  1:3083409/93‑1 (MQ=255)

                                           ggCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGNAATAGAAAATGCANANATAAtttt                                              >  1:1917883/1‑93 (MQ=255)

                                           ggCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAAtttt                                              <  2:3855947/93‑1 (MQ=255)

                                            gCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTa                                             <  2:1598210/93‑1 (MQ=255)

                                                tCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGata                                         >  2:2175416/1‑93 (MQ=255)

                                                tCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGata                                         <  2:4700853/93‑1 (MQ=255)

                                                 ccAACGCCCAAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGatat                                        <  1:2772914/93‑1 (MQ=255)

                                                 ccAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGatat                                        >  2:4183136/1‑93 (MQ=255)

                                                 ccAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGatat                                        <  2:4513724/93‑1 (MQ=255)

                                                  cAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATAtt                                       <  2:4854150/93‑1 (MQ=255)

                                                  cAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATAtt                                       <  2:1780577/93‑1 (MQ=255)

                                                    aCGACCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGa                                     <  2:6033499/93‑1 (MQ=255)

                                                       aCCCAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATAc                                  >  1:3290524/1‑93 (MQ=255)

                                                        cccAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACt                                 >  1:527409/1‑93 (MQ=255)

                                                         ccAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTc                                <  2:559370/93‑1 (MQ=255)

                                                         ccAATGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTc                                <  1:2560348/93‑1 (MQ=255)

                                                           aaTGTCACCAATATGCGTCAGGCACTCCTTAGGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCg                              >  1:5148874/1‑93 (MQ=255)

                                                             tGTCACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGga                            <  1:2335916/93‑1 (MQ=255)

                                                                cACCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGgacga                         >  2:4959957/1‑93 (MQ=255)

                                                                 aCCAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGgacgac                        >  1:2873904/1‑93 (MQ=255)

                                                                  ccAATATGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACt                       <  2:1738833/93‑1 (MQ=255)

                                                                      tatGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTttat                   <  2:39866/93‑1 (MQ=255)

                                                                      tatGCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTttat                   <  1:4975204/93‑1 (MQ=255)

                                                                         gCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTAc                <  1:1793471/93‑1 (MQ=255)

                                                                         gCGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTAc                >  2:5753712/1‑93 (MQ=255)

                                                                          cGTCAGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACa               <  2:2334578/93‑1 (MQ=255)

                                                                            tCAGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGc             >  1:5383637/1‑93 (MQ=255)

                                                                            tCAGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGc             >  1:5048743/1‑93 (MQ=255)

                                                                             cAGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCg            <  2:5769016/93‑1 (MQ=255)

                                                                              aGGCACTCCTTACGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGa           <  1:966689/93‑1 (MQ=255)

                                                                               ggCACTCCTTACGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGaa          >  2:1658076/1‑93 (MQ=255)

                                                                                gCACTCCTTACGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAg         <  2:620007/93‑1 (MQ=255)

                                                                                 cACTCCTTACGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAgg        <  2:1082937/93‑1 (MQ=255)

                                                                                   ctcctcACGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATGGATCCGCCGGACGACTTTATTACTGCGAAGGaa      <  1:2081333/93‑1 (MQ=255)

                                                                                    tCCTTACGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGaaa     <  1:28465/93‑1 (MQ=255)

                                                                                    cccTTACGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGaaa     <  2:1827428/92‑1 (MQ=255)

                                                                                     ccTTACGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAg    <  1:881378/93‑1 (MQ=255)

                                                                                      cTTACGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAgg   <  1:1826267/93‑1 (MQ=255)

                                                                                      cTTACGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAgg   <  2:778702/93‑1 (MQ=255)

TCCCCGGCCATCCGATTTTAGTGTCAAAACCATGCCTGATGCAGGCAATCCAACGACCCAATGTCACCAATATGCGTCAGGCACTCCTTATGGGAGATCATTATTCCGTCGAAATAGAAAATGCAGAAATAATTTTAGATATTGATACTCCGGACGACTTTATTACAGCGAAGGAAAGGT  >  NC_000913/3013566‑3013745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`