BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,116,492	C→T	E213K (GAA→AAA)	b2974 ←	putative endoglucanase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,116,492	0	C	T	100.0%	1105.1 / NA	281	E213K (GAA→AAA)	b2974	putative endoglucanase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (147/134); total (147/134)

Only 100 of 286 total aligned reads displayed.

CGGGCACGACCGGTGAAGTATGAGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCG  >  NC_000913/3116402‑3116581

 gggCACGACCGGTGAAGTATGAGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCgg                                                                                        <  2:5838027/93‑1 (MQ=255)

  ggCACGACCGGTGAAGTATGAGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGt                                                                                       <  1:4114335/93‑1 (MQ=255)

  ggCACGACCGGTGAAGTATGAGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGt                                                                                       >  2:6472498/1‑93 (MQ=255)

  ggCACGACCGGTGAAGTATGAGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGt                                                                                       <  1:3026188/93‑1 (MQ=255)

  ggCACGACCGGTGAAGTATGAGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGt                                                                                       <  2:5156381/93‑1 (MQ=255)

     aCGACCGGTGAAGTATGAGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGcc                                                                                    <  2:6348600/93‑1 (MQ=255)

     aCGACCGGTGAAGTATGAGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGcc                                                                                    >  1:5890627/1‑93 (MQ=255)

     aCGACCGGTGAAGTATGAGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGcc                                                                                    <  2:2929505/93‑1 (MQ=255)

     aCGACCGGTGAAGTATGAGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGcc                                                                                    >  1:3068388/1‑93 (MQ=255)

      cGACCGGTGAAGTATGAGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCa                                                                                   <  1:3258656/93‑1 (MQ=255)

         ccGGTGAAGTATGAGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGAt                                                                                <  2:3952967/93‑1 (MQ=255)

         ccGGTGAAGTATGAGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGAt                                                                                <  1:4568456/93‑1 (MQ=255)

         ccGGTGAAGTATGAGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGAt                                                                                <  2:2773243/93‑1 (MQ=255)

         ccGGTGAAGTATGAGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGAt                                                                                >  1:3897687/1‑93 (MQ=255)

             tGAAGTATGAGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGAt                                                                            <  1:5415889/93‑1 (MQ=255)

              gAAGTATGAGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGAtt                                                                           >  2:4343436/1‑93 (MQ=255)

              gAAGTATGAGTGGATGGCGCTTTATCGGGCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGAtt                                                                           >  1:631178/1‑93 (MQ=255)

               aaGTATGAGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGAttt                                                                          >  1:286816/1‑93 (MQ=255)

               aaGTATGAGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGAttt                                                                          >  2:1675484/1‑93 (MQ=255)

               aaGTATGAGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGAttt                                                                          >  2:1487534/1‑93 (MQ=255)

                   aTGAGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCtt                                                                      <  2:2546303/93‑1 (MQ=255)

                    tGAGTGGATGGCGTTTTATCGGTCGCAGCATTTTTTCCCACCTCTTCATTGACCAGCTTTTTGACCTTTTTCGGTGCCAGATCGATTTGCTta                                                                     <  1:2548558/93‑1 (MQ=255)

                    tGAGTGGATGGCGCTTTATCTGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTta                                                                     <  2:2715802/93‑1 (MQ=255)

                       gTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATAc                                                                  <  1:143212/93‑1 (MQ=255)

                        tGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACa                                                                 >  1:2087090/1‑93 (MQ=255)

                         ggATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAg                                                                <  2:2182366/93‑1 (MQ=255)

                          gATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGc                                                               <  1:5289253/93‑1 (MQ=255)

                           aTGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCg                                                              >  2:5932902/1‑93 (MQ=255)

                             ggCGCTTTATCTGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAg                                                            <  1:6077559/93‑1 (MQ=255)

                             ggCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAg                                                            <  1:5216793/93‑1 (MQ=255)

                               cgcTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTc                                                          >  2:597250/1‑93 (MQ=255)

                                gcTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCg                                                         >  1:6435245/1‑93 (MQ=255)

                                   ttATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAg                                                      <  2:29890/93‑1 (MQ=255)

                                   ttATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAg                                                      >  2:5552963/1‑93 (MQ=255)

                                    tATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGc                                                     <  1:6342765/93‑1 (MQ=255)

                                    tATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGc                                                     <  1:151208/93‑1 (MQ=255)

                                     aTCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCg                                                    >  1:2377497/1‑93 (MQ=255)

                                      tCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGt                                                   <  2:3214420/93‑1 (MQ=255)

                                      tCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGt                                                   <  1:162659/93‑1 (MQ=255)

                                       cGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGtt                                                  >  1:2931768/1‑93 (MQ=255)

                                       cGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGtt                                                  >  2:4764775/1‑93 (MQ=255)

                                       cGGTCGCAGCATTGTTTTCCACCTCTTCATTAACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGtt                                                  <  1:4226581/93‑1 (MQ=255)

                                        ggTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGttt                                                 >  2:3005508/1‑93 (MQ=255)

                                         gTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGtttc                                                >  2:1432460/1‑92 (MQ=255)

                                         gTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTa                                                <  1:5440066/93‑1 (MQ=255)

                                          tCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTAc                                               >  1:102063/1‑93 (MQ=255)

                                          tCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTAc                                               >  2:840325/1‑93 (MQ=255)

                                            gcagcaTTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTActc                                             >  1:2733673/1‑93 (MQ=255)

                                            gcagcaTTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTActc                                             >  1:1268823/1‑93 (MQ=255)

                                              agcaTTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACtctc                                           <  2:4002425/93‑1 (MQ=255)

                                              agcaTTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACtctc                                           >  1:734339/1‑93 (MQ=255)

                                              agcaTTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACtctc                                           >  1:1185449/1‑93 (MQ=255)

                                              agcaTTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACtctc                                           <  2:4541611/93‑1 (MQ=255)

                                              agcaTTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACtctc                                           <  1:4675289/93‑1 (MQ=255)

                                               gcaTTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCg                                          <  2:1056942/93‑1 (MQ=255)

                                               gcaTTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCg                                          >  2:3666392/1‑93 (MQ=255)

                                                 aTTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGAt                                        <  1:5731547/93‑1 (MQ=255)

                                                 aTTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGAt                                        <  1:4377072/93‑1 (MQ=255)

                                                  ttGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATc                                       <  1:594806/93‑1 (MQ=255)

                                                   tGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCa                                      <  2:3394466/93‑1 (MQ=255)

                                                    gTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCAc                                     <  1:3705302/93‑1 (MQ=255)

                                                     ttttCCACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCAcc                                    >  2:5101037/1‑93 (MQ=255)

                                                         ccACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCgagg                                >  1:2415880/1‑93 (MQ=255)

                                                          cACCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCgagga                               >  2:5713856/1‑93 (MQ=255)

                                                           cccTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCgaggag                              <  2:1175973/92‑1 (MQ=255)

                                                           aCCTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGGGTTTACTCTCGATCACCgaggag                              >  1:1822971/1‑93 (MQ=255)

                                                            ccTCTTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGgaga                             >  2:2276569/1‑93 (MQ=255)

                                                             ctctTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGaa                            >  1:3456323/1‑93 (MQ=255)

                                                             ctctTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGaa                            >  1:5162442/1‑93 (MQ=255)

                                                             ctctTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGaa                            >  2:2407904/1‑93 (MQ=255)

                                                             ctctTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGaa                            >  1:215644/1‑93 (MQ=255)

                                                             cactTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGCGaa                            >  1:319573/3‑93 (MQ=255)

                                                               ctTCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACg                          >  2:503377/1‑93 (MQ=255)

                                                                 tCATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTc                        <  1:4915447/93‑1 (MQ=255)

                                                                  cATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCa                       >  2:1999801/1‑93 (MQ=255)

                                                                  cATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCa                       >  2:3612847/1‑93 (MQ=255)

                                                                  cATTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCa                       <  2:1362907/93‑1 (MQ=255)

                                                                   aTTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAg                      >  2:1773716/1‑93 (MQ=255)

                                                                   aTTGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAg                      >  2:80471/1‑93 (MQ=255)

                                                                    ttGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGa                     >  1:1387798/1‑93 (MQ=255)

                                                                    ttGACCAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGa                     >  2:4495096/1‑93 (MQ=255)

                                                                        ccAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACaaa                 <  1:1169313/93‑1 (MQ=255)

                                                                        ccAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACaaa                 >  1:5058688/1‑93 (MQ=255)

                                                                         cAGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAAc                <  1:4783628/93‑1 (MQ=255)

                                                                          aGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAAcc               >  1:569536/1‑93 (MQ=255)

                                                                          aGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAAcc               >  1:1217974/1‑93 (MQ=255)

                                                                          aGCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAAcc               <  2:1238543/93‑1 (MQ=255)

                                                                           gCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCt              <  2:6169169/93‑1 (MQ=255)

                                                                           gCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCt              >  2:2305471/1‑93 (MQ=255)

                                                                           gCTTTTTGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCt              >  1:1496377/1‑93 (MQ=255)

                                                                             tttttGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGt            >  2:5388096/1‑93 (MQ=255)

                                                                              ttttGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGtt           <  1:414169/93‑1 (MQ=255)

                                                                                ttGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCt         >  2:214642/1‑93 (MQ=255)

                                                                                 tGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTg        <  1:6168229/93‑1 (MQ=255)

                                                                                 tGAACTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTg        >  2:3117741/1‑93 (MQ=255)

                                                                                   aaCTCTTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTa      >  1:895092/1‑93 (MQ=255)

                                                                                     ctctTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTAtt    >  2:5914603/1‑93 (MQ=255)

                                                                                     ctctTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTAtt    >  2:4311530/1‑93 (MQ=255)

                                                                                      tctTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTc   >  1:3583135/1‑93 (MQ=255)

                                                                                      tctTTCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTc   >  1:224039/1‑93 (MQ=255)

CGGGCACGACCGGTGAAGTATGAGTGGATGGCGCTTTATCGGTCGCAGCATTGTTTTCCACCTCTTCATTGACCAGCTTTTTGAACTCTTCCGGTGCCAGATCGATTTGCTTATACAGCGAGTCGAAGCGTTTACTCTCGATCACCGAGGAGAACGTCAGACAAACCTGTTCTGTATTCG  >  NC_000913/3116402‑3116581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`