BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,132,550	T→C	G135G (GGT→GGC)	yghT →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,132,550	0	T	C	99.5%	747.2 / ‑4.9	219	G135G (GGT→GGC)	yghT	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (119/99); total (120/99)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

Only 100 of 240 total aligned reads displayed.

TCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTCAA  >  NC_000913/3132460‑3132639

tcgCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGCACGGTGTTGCCACCTAACGTTGAAggcgg                                                                                         >  2:2029026/1‑93 (MQ=255)

 cgcgGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAggcggt                                                                                        <  2:2635784/93‑1 (MQ=255)

   cgGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCg                                                                                      >  1:1215587/1‑93 (MQ=255)

   cgGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCg                                                                                      >  1:6050425/1‑93 (MQ=255)

    gggTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGt                                                                                     >  1:1581776/1‑93 (MQ=255)

      gTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGttt                                                                                   <  2:3933200/93‑1 (MQ=255)

      gTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGttt                                                                                   >  2:4139757/1‑93 (MQ=255)

        tAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTg                                                                                 >  1:2856976/1‑93 (MQ=255)

         aaTTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGt                                                                                >  2:2972099/1‑93 (MQ=255)

         aaTTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGt                                                                                >  1:4330929/1‑93 (MQ=255)

          aTTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGtc                                                                               >  2:1337350/1‑93 (MQ=255)

          aTTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGtc                                                                               <  2:5613627/93‑1 (MQ=255)

            tGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGtctc                                                                             <  2:4327807/93‑1 (MQ=255)

               tCTAGCCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCTTTTTGTCTCAtg                                                                          <  1:4239431/93‑1 (MQ=255)

               tCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCAtg                                                                          >  1:222194/1‑93 (MQ=255)

               tCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCAtg                                                                          <  1:5626102/93‑1 (MQ=255)

                cTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCAtgg                                                                         >  2:667291/1‑93 (MQ=255)

                 tAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCAtggc                                                                        >  1:5951071/1‑93 (MQ=255)

                   accgaccgTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCAtggctg                                                                      <  2:5268796/93‑1 (MQ=255)

                        ccgTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAg                                                                 >  1:529518/1‑93 (MQ=255)

                         cgTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGc                                                                <  2:492274/93‑1 (MQ=255)

                          gTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGcc                                                               <  2:1156640/93‑1 (MQ=255)

                          gTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGcc                                                               <  1:3400044/93‑1 (MQ=255)

                          gTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGcc                                                               <  2:2753567/93‑1 (MQ=255)

                            tagccTCAGGACCAAATGCCTGGCTCTTACGATGGTACGGTGTTCCCGCCTAACGTTGAAGGCGGTCGTTGTGTCTCATGGCTGGCAAGCCAg                                                             <  1:3216012/90‑1 (MQ=255)

                             aTCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAgg                                                            >  1:6213453/1‑93 (MQ=255)

                             aTCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAgg                                                            >  1:1193963/1‑93 (MQ=255)

                             aTCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAgg                                                            <  2:2642069/93‑1 (MQ=255)

                               ccTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGaa                                                          <  1:3760071/93‑1 (MQ=255)

                               ccTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGaa                                                          >  2:5911494/1‑93 (MQ=255)

                               ccTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGaa                                                          <  1:4483217/93‑1 (MQ=255)

                                cTCAGGCCCAAATTCCTGGCTCTTACGATGGTACGGTGTTCCCGCCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAAc                                                         <  1:2456/93‑1 (MQ=255)

                                cTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAAc                                                         <  2:179644/93‑1 (MQ=255)

                                cTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAAc                                                         <  1:883771/93‑1 (MQ=255)

                                 tCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACg                                                        >  2:3757170/1‑93 (MQ=255)

                                  cAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGt                                                       >  1:2574843/1‑93 (MQ=255)

                                   aGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTa                                                      >  1:6184359/1‑93 (MQ=255)

                                    ggACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTaa                                                     >  1:4160054/1‑93 (MQ=255)

                                    ggACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTaa                                                     >  2:5464246/1‑93 (MQ=255)

                                      aCCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAg                                                   <  2:483452/93‑1 (MQ=255)

                                       ccAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGc                                                  >  1:3935933/1‑93 (MQ=255)

                                       ccAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGc                                                  <  2:2064103/93‑1 (MQ=255)

                                       ccAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGc                                                  <  2:2828764/93‑1 (MQ=255)

                                       ccAAATTCCTGGCGCTTACGATGGTACGGTGTTCCAACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGc                                                  <  1:1746368/93‑1 (MQ=255)

                                        cAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCg                                                 >  2:4515123/1‑93 (MQ=255)

                                        cAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCg                                                 >  2:5511326/1‑93 (MQ=255)

                                         aaaTTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGt                                                >  1:1018088/1‑93 (MQ=255)

                                         aaaTTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGt                                                >  1:2983855/1‑93 (MQ=255)

                                         aaaTTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGt                                                <  1:2108462/93‑1 (MQ=255)

                                           aTTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGtgt                                              <  1:1938921/93‑3 (MQ=255)

                                               cTGGCGCTTACGATGGTACGGTGTTCCCTCCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACt                                          <  1:845899/93‑1 (MQ=255)

                                               cTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGGGTTTCACt                                          >  2:3712679/1‑93 (MQ=255)

                                               cTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACt                                          >  1:2312265/1‑93 (MQ=255)

                                                tGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTg                                         >  1:5461507/1‑93 (MQ=255)

                                                tGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTg                                         <  1:4862230/93‑1 (MQ=255)

                                                 ggCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTgg                                        >  2:5058334/1‑93 (MQ=255)

                                                   cgcTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGAt                                      <  2:3555247/93‑1 (MQ=255)

                                                   cgcTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGAt                                      >  1:382861/1‑93 (MQ=255)

                                                      ttACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGc                                   <  2:5822689/93‑1 (MQ=255)

                                                       tACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCg                                  <  2:3408061/93‑1 (MQ=255)

                                                       tACGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCg                                  <  1:3075240/93‑1 (MQ=255)

                                                         cGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAg                                >  2:5000781/1‑93 (MQ=255)

                                                         cGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAg                                >  2:737851/1‑93 (MQ=255)

                                                         cGATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAg                                >  1:6109744/1‑93 (MQ=255)

                                                          gATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGc                               >  1:3667097/1‑93 (MQ=255)

                                                          gATGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGc                               <  2:2553591/93‑1 (MQ=255)

                                                           aTGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAggc                              >  2:5428926/1‑91 (MQ=255)

                                                           aTGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGcc                              <  1:4515123/93‑1 (MQ=255)

                                                            tGGTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCa                             >  2:3096058/1‑93 (MQ=255)

                                                             ggTACGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCAt                            >  1:21322/1‑93 (MQ=255)

                                                                 cgttgtTCCCCCTTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGc                        <  1:3471785/90‑1 (MQ=255)

                                                                 cGGTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGGACGTAAAGCGTTTCACTTGATGGCGCGCCATGAGc                        >  2:3765808/1‑93 (MQ=255)

                                                                  gttgtTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGcc                       <  1:6097717/91‑1 (MQ=255)

                                                                  ggTGTTCCCCCCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGcc                       <  1:3299345/93‑1 (MQ=255)

                                                                  ggTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGcc                       <  1:6374699/93‑1 (MQ=255)

                                                                  ggTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGcc                       >  2:4019611/1‑93 (MQ=255)

                                                                  ggTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGcc                       <  2:6228923/93‑1 (MQ=255)

                                                                  ggTGTTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGcc                       >  2:4961571/1‑93 (MQ=255)

                                                                   gtgtTCCCACCTAACGTTGAAGGGGGTCGTTTTGTCTCAGGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCt                      <  1:6412945/93‑1 (MQ=255)

                                                                   gtgtTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCt                      <  1:1445402/93‑1 (MQ=255)

                                                                    tgtTCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTg                     <  1:4649324/93‑1 (MQ=255)

                                                                      ttCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGGCATAAGCCTGAt                   >  2:107376/1‑93 (MQ=255)

                                                                      ttCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGAt                   >  2:3635097/1‑93 (MQ=255)

                                                                       tCCCACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATc                  <  2:2500318/93‑1 (MQ=255)

                                                                         ccACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTg                <  1:4569642/93‑1 (MQ=255)

                                                                         ccACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTg                <  2:4454634/93‑1 (MQ=255)

                                                                          cACCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTgg               <  1:5272632/93‑1 (MQ=255)

                                                                           cccTACCGTTGAAGGCGTTTTTTTTGTCCCATGGCTGACAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGt              <  1:1123266/92‑1 (MQ=255)

                                                                           aCCTAGCGTTGAAGTTGATCGTTTTGTCACATGGCAGCCAAGCCAGGAACGTAAAGCTTTTCACTGAATGGCGAGCCATAAGCCTGATCTGGt              <  2:3986017/93‑1 (MQ=255)

                                                                           aCCTAACGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGt              >  2:4259642/1‑93 (MQ=255)

                                                                                aCGTTGAAGGCGTTCGTTTTGTCTCATGGCGGGCAAGCGAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCCGGtcatca         <  1:6270662/93‑1 (MQ=255)

                                                                                 cGTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCaa        >  2:2347726/1‑93 (MQ=255)

                                                                                  gTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCaaa       >  2:167787/1‑93 (MQ=255)

                                                                                  gTTGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCaaa       >  2:6066883/1‑93 (MQ=255)

                                                                                   ttGAAGGGGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAAc      <  2:4718426/93‑1 (MQ=255)

                                                                                   ttGAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAAc      >  1:5217358/1‑93 (MQ=255)

                                                                                     gAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTc    >  1:5431274/1‑93 (MQ=255)

                                                                                     gAAGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTc    >  2:1653501/1‑93 (MQ=255)

                                                                                      aaGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTCa   >  1:1268527/1‑93 (MQ=255)

                                                                                       aGGCGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTCaa  >  1:4100096/1‑93 (MQ=255)

TCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTACGGTGTTCCCACCTAACGTTGAAGGTGGTCGTTTTGTCTCATGGCTGGCAAGCCAGGAACGTAAAGCGTTTCACTGGATGGCGAGCCATAAGCCTGATCTGGTCATCAAACTCAA  >  NC_000913/3132460‑3132639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`