BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,158,281	C→T	L294L (CTG→CTA)	ygiRQ ←	Fe‑S oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,158,281	0	C	T	99.3%	1030.8 / ‑3.9	276	L294L (CTG→CTA)	ygiRQ	Fe‑S oxidoreductase
Reads supporting (aligned to +/- strand): ref base C (2/0); new base T (131/142); total (134/142)
Fisher's exact test for biased strand distribution p-value = 2.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01

Only 100 of 289 total aligned reads displayed.

GCGGGCACAG‑CTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCGGTTT  >  NC_000913/3158192‑3158370

 cgGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGgtagc                                                                                         >  2:5890818/1‑93 (MQ=255)

 cgGGCACAGCCTGGGTTGGCTTCGTGGTGCAGAATACGGGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGgtagc                                                                                         <  1:5230423/93‑1 (MQ=255)

  gggCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGgtagca                                                                                        >  2:1511033/1‑93 (MQ=255)

  gggCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGgtagca                                                                                        >  1:4487847/1‑93 (MQ=255)

    gCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAac                                                                                      <  2:3525161/93‑1 (MQ=255)

       cagccTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACg                                                                                   >  2:6092547/5‑93 (MQ=255)

        agccTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGt                                                                                  <  1:618111/90‑1 (MQ=255)

        agccTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGt                                                                                  <  1:2456306/90‑1 (MQ=255)

         gccTGGGTTGGTTTCGTTGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTa                                                                                 >  1:6160763/3‑93 (MQ=255)

         gccTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTa                                                                                 >  1:3180023/3‑93 (MQ=255)

          ccTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAg                                                                                >  1:5997024/2‑93 (MQ=255)

           cTGGGTTGTTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAgg                                                                               <  2:3328661/93‑1 (MQ=255)

           cTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAgg                                                                               <  2:1240687/93‑1 (MQ=255)

           cTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAgg                                                                               >  2:11464/1‑93 (MQ=255)

           cTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAgg                                                                               <  2:3269925/93‑1 (MQ=255)

            tGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGt                                                                              >  2:2513186/1‑93 (MQ=255)

            tGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGt                                                                              >  1:4303318/1‑93 (MQ=255)

             gggTTGGTTTCGTAGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGtt                                                                             <  1:3697359/93‑1 (MQ=255)

               gttggttTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGtttt                                                                           >  1:4155929/1‑93 (MQ=255)

                  ggttTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTCCTCGAAAGAAGGTAGCAACCCGTAGGTTTTTTc                                                                        <  1:4466891/93‑1 (MQ=255)

                    tttCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTccc                                                                      <  2:3414496/93‑1 (MQ=255)

                    tttCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTccc                                                                      <  1:4645874/93‑1 (MQ=255)

                      tCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCAc                                                                    <  2:6182725/93‑1 (MQ=255)

                      tCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCAc                                                                    <  1:1910417/93‑1 (MQ=255)

                       cGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACg                                                                   >  2:2724367/1‑93 (MQ=255)

                        gtggtgCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACgg                                                                  >  1:1763349/1‑93 (MQ=255)

                        gtggtgCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACgg                                                                  <  1:2618005/93‑1 (MQ=255)

                          ggtgCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGtt                                                                >  1:1819213/1‑93 (MQ=255)

                           gtgCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGttt                                                               <  1:4729724/93‑1 (MQ=255)

                            tgCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTc                                                              <  1:597281/93‑1 (MQ=255)

                             gCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑g                                                            >  1:3606259/1‑93 (MQ=255)

                               aGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑ggg                                                          <  2:2866281/93‑1 (MQ=255)

                               aGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑ggg                                                          >  1:399522/1‑93 (MQ=255)

                               aGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑ggg                                                          >  1:2980248/1‑93 (MQ=255)

                                gAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑gggg                                                         >  2:2666661/1‑92 (MQ=255)

                                 aaTACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGgcg                                                        <  2:4174266/93‑1 (MQ=255)

                                 aaTACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGgcg                                                        <  1:496221/93‑1 (MQ=255)

                                  aTACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCACACGGTTTCTGGgcg                                                        >  1:1676728/1‑93 (MQ=255)

                                   tACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGcgcg                                                      >  2:3793517/1‑93 (MQ=255)

                                   tACGCGAAGCATGGGCATACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGcgcg                                                      <  2:5276424/93‑1 (MQ=255)

                                     cgcgAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGt                                                    <  2:727921/93‑1 (MQ=255)

                                       cgAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTgg                                                  >  2:5514385/1‑93 (MQ=255)

                                        gAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGc                                                 <  2:876119/93‑1 (MQ=255)

                                        gAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGc                                                 >  1:2199914/1‑93 (MQ=255)

                                         aaGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCt                                                >  1:5084341/1‑93 (MQ=255)

                                         aaGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCt                                                >  2:1782916/1‑93 (MQ=255)

                                          aGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTg                                               <  1:5820079/93‑1 (MQ=255)

                                           gCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGc                                              >  2:5838575/1‑93 (MQ=255)

                                            gaTGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGCa                                             <  1:55900/92‑1 (MQ=255)

                                            cATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGCa                                             <  1:6266723/93‑1 (MQ=255)

                                             aTGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGCAc                                            <  2:3703677/93‑1 (MQ=255)

                                             aTGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGCAc                                            >  1:6477789/1‑93 (MQ=255)

                                              tGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGCACg                                           >  2:602840/1‑93 (MQ=255)

                                              tGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGCGGCTGCACg                                           >  2:594899/1‑93 (MQ=255)

                                               gggCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGCACgg                                          >  1:3349330/1‑93 (MQ=255)

                                                ggCGTACAGCCCTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAGCACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGCACGGt                                         <  1:1335902/93‑1 (MQ=255)

                                                ggCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGCACGGt                                         >  2:6440789/1‑93 (MQ=255)

                                                      cAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGCACGGTTACGGc                                   <  2:5037655/93‑1 (MQ=255)

                                                       aGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGCACGGTTACGGCt                                  >  1:5439114/1‑93 (MQ=255)

                                                       aGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGCACGGTTACGGCt                                  <  1:1422222/93‑1 (MQ=255)

                                                        gCACTTTATCGCTCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGCACGGTTACGGCtt                                 <  1:3915519/93‑1 (MQ=255)

                                                        gCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGCACGGTTACGGCtt                                 >  2:3642110/1‑93 (MQ=255)

                                                        gCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGCACGGTTACGGCtt                                 <  2:2034151/93‑1 (MQ=255)

                                                          aCTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGCACGGTTACGGCTTTg                               >  2:2135195/1‑93 (MQ=255)

                                                          aCTTTATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGCACGGTTACGGCTTTg                               <  2:5615866/93‑1 (MQ=255)

                                                            tttATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGCACGGTTACGGCTTTGGc                             >  2:1564279/1‑93 (MQ=255)

                                                             ttATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGCACGGTTACGGCTTTGGCt                            <  1:2147746/93‑1 (MQ=255)

                                                             ttATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGCACGGTTACGGCTTTGGCt                            <  1:5679436/93‑1 (MQ=255)

                                                              tATCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGCACGGGTACGGCTTTGGCtt                           >  2:5680943/1‑93 (MQ=255)

                                                                tCGCCCTTCACTTTCTCGAAAGAAGGTAGTAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTcc                         <  1:1025892/93‑1 (MQ=255)

                                                                tCGCCCTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTcc                         >  2:207411/1‑93 (MQ=255)

                                                                     cTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCtt                    >  2:5445327/1‑93 (MQ=255)

                                                                     cTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCtt                    <  2:4511759/93‑1 (MQ=255)

                                                                     cTTCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCtt                    >  1:6128924/1‑93 (MQ=255)

                                                                      ttCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCttt                   <  1:6413725/93‑1 (MQ=255)

                                                                      ttCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCttt                   <  1:6092999/93‑1 (MQ=255)

                                                                       tCACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCtttt                  <  1:5460413/93‑1 (MQ=255)

                                                                        cACTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCttttt                 >  2:4816461/1‑93 (MQ=255)

                                                                         aCTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTc                <  1:5619145/93‑1 (MQ=255)

                                                                         aCTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTc                <  1:2737260/93‑1 (MQ=255)

                                                                         aCTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTc                >  1:3257612/1‑93 (MQ=255)

                                                                         aCTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTc                <  2:5142031/93‑1 (MQ=255)

                                                                          cTTTCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCg               >  2:1849919/1‑93 (MQ=255)

                                                                            ttCTCGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGt             <  2:4303318/93‑1 (MQ=255)

                                                                             tctcGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTg            >  2:2607080/1‑93 (MQ=255)

                                                                               tcGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGcc          >  1:4410297/1‑93 (MQ=255)

                                                                               tcGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGcc          <  2:296826/93‑1 (MQ=255)

                                                                               tcGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGcc          >  1:4371100/1‑93 (MQ=255)

                                                                                cGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCa         <  2:5784125/93‑1 (MQ=255)

                                                                                cGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCa         >  2:5109096/1‑93 (MQ=255)

                                                                                cGAAAGAAGGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCa         <  2:5371349/93‑1 (MQ=255)

                                                                                    agaagGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTTGGTTCCAcccg     >  1:5877011/1‑91 (MQ=255)

                                                                                    agaagGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCgg     >  1:833740/1‑93 (MQ=255)

                                                                                    agaagGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCgg     <  1:2800503/93‑1 (MQ=255)

                                                                                    agaagGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCgg     <  2:2281657/93‑1 (MQ=255)

                                                                                     gaagGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCGGt    >  2:6412295/1‑93 (MQ=255)

                                                                                     gaagGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCGGt    >  2:1395739/1‑93 (MQ=255)

                                                                                      aagGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCGGtt   <  1:3670800/93‑1 (MQ=255)

                                                                                      aagGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCGGtt   <  2:3998541/93‑1 (MQ=255)

                                                                                       agGTAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTCGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCGGttt  <  1:3102647/93‑1 (MQ=255)

GCGGGCACAG‑CTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAGGTTTTTTCCCACGGTTTC‑GGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCGGTTT  >  NC_000913/3158192‑3158370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`