BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,225,194	C→T	V440V (GTC→GTT)	ygjI →	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,225,194	0	C	T	99.4%	633.1 / ‑4.9	164	V440V (GTC→GTT)	ygjI	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (74/89); total (74/90)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.56e-01

Only 100 of 178 total aligned reads displayed.

TTCAATGCTGATTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTCGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGATTAACGGCTGAAGAGCGCCATATTGAAG  >  NC_000913/3225104‑3225282

ttCAATGCTGATTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTtgg                                                                                        >  2:158266/1‑93 (MQ=255)

ttCAATGCTGATTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTtgg                                                                                        >  1:1992936/1‑93 (MQ=255)

   aaTGCTGATTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTtggcgg                                                                                     >  2:2543600/1‑93 (MQ=255)

   aaTGCTGATTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTtggcgg                                                                                     <  2:5891587/93‑1 (MQ=255)

   aaTGCTGATTGCGATATTTGCCGTAGGGGTTGTCGCTTCGACATTCCCGACTGGCGCGACTATTCTGACCAGCATTTTTTATAACGTtggcgg                                                                                     >  1:2676131/1‑93 (MQ=255)

    aTGCTGATTGCGATATTTGCCGTAGGGTTTGTCACTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGt                                                                                    <  1:4966834/93‑1 (MQ=255)

     tGCTGATTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTa                                                                                   >  1:5504096/1‑93 (MQ=255)

     tGCTGATTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTa                                                                                   >  1:1462550/1‑93 (MQ=255)

     tGCTGATTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTa                                                                                   <  1:4520758/93‑1 (MQ=255)

     tGCTGATTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGGTGGCGGTa                                                                                   <  1:6295638/93‑1 (MQ=255)

       cTGATTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTAtt                                                                                 <  2:776325/93‑1 (MQ=255)

        tGATTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTg                                                                                >  2:3702007/1‑93 (MQ=255)

         taTTGCGATATTTGCCGTAGTGTTGGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGt                                                                               <  1:6146916/92‑1 (MQ=255)

          aTTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGtt                                                                              >  2:66352/1‑93 (MQ=255)

           ttGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTa                                                                             <  2:510009/93‑1 (MQ=255)

            tGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTAt                                                                            >  1:3357137/1‑93 (MQ=255)

             gctatatTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATc                                                                           <  1:3463492/90‑1 (MQ=255)

             gCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATc                                                                           <  2:1063442/93‑1 (MQ=255)

              cGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCt                                                                          <  2:3392200/93‑1 (MQ=255)

               gATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCtt                                                                         >  2:4569401/1‑93 (MQ=255)

               gATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCtt                                                                         >  2:3984402/1‑93 (MQ=255)

               gATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCtt                                                                         >  1:588858/1‑93 (MQ=255)

                atatTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTc                                                                        <  1:374512/93‑1 (MQ=255)

                  atTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCt                                                                      <  2:5778828/93‑1 (MQ=255)

                  atTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCt                                                                      <  1:4841655/93‑1 (MQ=255)

                    ttGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTg                                                                    <  2:136343/93‑1 (MQ=255)

                     tGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTgg                                                                   <  2:1455168/93‑1 (MQ=255)

                     tGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTgg                                                                   <  2:2198538/93‑1 (MQ=255)

                       ccGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCt                                                                 <  1:1561362/93‑1 (MQ=255)

                       ccGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCt                                                                 >  1:2025952/1‑93 (MQ=255)

                        cGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCtt                                                                >  1:6091509/1‑93 (MQ=255)

                        cGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCtt                                                                <  2:514647/93‑1 (MQ=255)

                        cGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCtt                                                                <  1:5580062/93‑1 (MQ=255)

                            gggTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCg                                                            >  1:4325330/1‑93 (MQ=255)

                            gggTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCg                                                            <  2:3822140/93‑1 (MQ=255)

                             ggTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCgt                                                           >  1:165013/1‑93 (MQ=255)

                             ggTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCgt                                                           >  2:5736237/1‑93 (MQ=255)

                              gTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCgtg                                                          <  1:1142115/93‑1 (MQ=255)

                               tttGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCgtgg                                                         <  1:1327250/93‑1 (MQ=255)

                                   tCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGa                                                     >  2:1616074/1‑93 (MQ=255)

                                    cGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTTGaa                                                    >  2:11356/1‑93 (MQ=255)

                                     gCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGaaa                                                   <  1:3587710/93‑1 (MQ=255)

                                     gCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGaaa                                                   >  2:6047261/1‑93 (MQ=255)

                                      cTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATTTTCCTTGGCTTTGCGTGGTGGAAAt                                                  >  1:5692671/1‑93 (MQ=255)

                                       ttCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATa                                                 <  2:1076844/93‑1 (MQ=255)

                                       ttCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATa                                                 <  2:1999797/93‑1 (MQ=255)

                                         cGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACa                                               <  1:6249968/93‑1 (MQ=255)

                                          gACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAg                                              >  2:886506/1‑93 (MQ=255)

                                           aCATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGt                                             <  2:4901435/93‑1 (MQ=255)

                                            cATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTa                                            <  1:3982675/93‑1 (MQ=255)

                                            cATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTa                                            <  2:2271008/93‑1 (MQ=255)

                                            cATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTa                                            <  1:1200275/93‑1 (MQ=255)

                                             aTTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTaa                                           >  1:483569/1‑93 (MQ=255)

                                              ttCCCGACTGGCGCTAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTaaa                                          <  2:4555166/93‑1 (MQ=255)

                                              ttCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTaaa                                          <  1:2117084/93‑1 (MQ=255)

                                               tCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAat                                         >  1:3690740/1‑93 (MQ=255)

                                                 ccGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAatat                                       >  2:1691825/1‑93 (MQ=255)

                                                   gACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAatatat                                     >  2:5718120/1‑93 (MQ=255)

                                                   gACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTAGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAatatat                                     >  1:5247317/1‑93 (MQ=255)

                                                     cTGGCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATaa                                   >  1:3961588/1‑93 (MQ=255)

                                                       ggCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAg                                 <  1:1763994/93‑1 (MQ=255)

                                                       ggCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAg                                 <  1:2745000/93‑1 (MQ=255)

                                                       ggCGCGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAg                                 >  2:185404/1‑93 (MQ=255)

                                                        gcgcGAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAgg                                <  1:3563100/93‑1 (MQ=255)

                                                         cgcgAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAggg                               <  1:2968052/93‑1 (MQ=255)

                                                          gcgAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGa                              <  1:443295/93‑1 (MQ=255)

                                                          gcgAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGa                              <  2:14057/93‑1 (MQ=255)

                                                           cgAATATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGAt                             <  2:5629159/93‑1 (MQ=255)

                                                             aaTATTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGATTa                           >  1:5805583/1‑93 (MQ=255)

                                                               tatTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGATTAAc                         <  2:1955520/93‑1 (MQ=255)

                                                               tatTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGATTAAc                         <  1:2207661/93‑1 (MQ=255)

                                                                atTCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGATTAACg                        >  2:1322631/1‑93 (MQ=255)

                                                                 ttCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGATTAACgg                       <  2:3576836/93‑1 (MQ=255)

                                                                  tCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAACTATATAAAGGGATTAACGGc                      >  2:2904305/1‑93 (MQ=255)

                                                                  tCTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGATTAACGGc                      >  1:1574436/1‑93 (MQ=255)

                                                                   cTGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGATTAACGGCt                     <  1:430164/93‑1 (MQ=255)

                                                                    tGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGATTAACGGCTg                    >  1:3926972/1‑93 (MQ=255)

                                                                    tGACCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGATTAACGGCTg                    >  1:5848763/1‑93 (MQ=255)

                                                                      aCCATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGATTAACGGCTGaa                  <  1:2443943/93‑1 (MQ=255)

                                                                       ccATCATTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGATTAACGGCTGAag                 <  2:1818016/93‑1 (MQ=255)

                                                                        catcatTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGATTAACGGCTGAaga                >  2:1731319/1‑93 (MQ=255)

                                                                         atcatTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGATTAACGGCTGAagag               >  2:4373290/1‑93 (MQ=255)

                                                                         atcatTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGATTAACGGCTGAagag               >  1:5224845/1‑93 (MQ=255)

                                                                         atcatTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGGGGAAATACAGTAAATATATAAAGGGATTAACGGCTGAagag               >  2:279593/1‑93 (MQ=255)

                                                                          tcatTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGATTAACGGCTGAAGAgc              >  2:2125297/1‑93 (MQ=255)

                                                                          tcatTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGATTAACGGCTGAAGAgc              <  2:4469017/93‑1 (MQ=255)

                                                                           catTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGATTAACGGCTGAAGAgcg             >  2:2418191/1‑93 (MQ=255)

                                                                            atTTTTTATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGATTAACGGCTGAAGAgcgc            <  1:5978873/93‑1 (MQ=255)

                                                                             ttttttATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGATTAACGGCTGAAGAGCGcc           >  1:677582/1‑93 (MQ=255)

                                                                              tttttATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGATTAACGGCTGAAGAGTGCCa          >  1:4567928/1‑93 (MQ=255)

                                                                               ttttATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGATTAACGGCTGAAGAGCGCCat         <  1:2297099/93‑1 (MQ=255)

                                                                                tttATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGATTAACGGCTGAAGAGCGCCata        >  2:6358120/1‑93 (MQ=255)

                                                                                tttATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGATTAACGGCTGAAGAGCGCCata        <  2:1468993/93‑1 (MQ=255)

                                                                                 ttATAACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGATTAACGGCTGAAGAGCGCCatat       <  2:3212813/93‑1 (MQ=255)

                                                                                  tataACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGATTAACGGCTGAAGAGCGCCATAtt      <  2:362342/93‑1 (MQ=255)

                                                                                  tataACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGATTAACGGCTGAAGAGCGCCATAtt      <  2:1603377/93‑1 (MQ=255)

                                                                                    taACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGATTAACGGCTGAAGAGCGCCATAttaa    >  2:5389373/1‑91 (MQ=255)

                                                                                    taACGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGATTAACGGCTGAAGAGCGCCATATTGa    >  2:6364897/1‑93 (MQ=255)

                                                                                     aaCGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGATTAACGGCTGAAGAGCGCCATATTGaa   <  1:4713506/93‑1 (MQ=255)

                                                                                      aCGTTGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGATTAACGGCTGAAGAGCGCCATATTGAAg  <  1:2058313/93‑1 (MQ=255)

TTCAATGCTGATTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTTATAACGTCGGCGGTATTGTTATCTTCCTTGGCTTTGCGTGGTGGAAATACAGTAAATATATAAAGGGATTAACGGCTGAAGAGCGCCATATTGAAG  >  NC_000913/3225104‑3225282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`