BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	323,407	A→G	G142G (GGA→GGG)	ykgG →	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	323,407	0	A	G	99.6%	810.4 / NA	236	G142G (GGA→GGG)	ykgG	putative transporter
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (109/126); minor base T (0/1); total (109/127)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 245 total aligned reads displayed.

GGATCCGGCGAAAGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTAT  >  NC_000913/323317‑323496

ggATCCGGCGAAAGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCgggggg                                                                                         <  2:5311315/93‑1 (MQ=255)

 gATCCGGCGAAAGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGc                                                                                        <  2:6395358/93‑1 (MQ=255)

 gATCCGGCGAAAGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGc                                                                                        >  1:2006526/1‑93 (MQ=255)

  aTCCGGCGAAAGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCg                                                                                       <  1:527111/93‑1 (MQ=255)

   tCCGGCGAAAGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGt                                                                                      >  1:6373443/1‑93 (MQ=255)

    ccGGCGAAAGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTg                                                                                     >  2:5533966/1‑93 (MQ=255)

    ccGGCGAAAGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTg                                                                                     >  1:788804/1‑93 (MQ=255)

      ggCGAAAGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGt                                                                                   <  1:4749804/93‑1 (MQ=255)

      ggCGAAAGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGt                                                                                   <  2:3626279/93‑1 (MQ=255)

      ggCGAAAGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGt                                                                                   <  1:1707614/93‑1 (MQ=255)

         gAAAGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCt                                                                                >  2:2756349/1‑93 (MQ=255)

          aaaGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCtt                                                                               >  2:1529684/1‑93 (MQ=255)

          aaaGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCtt                                                                               <  1:3288533/93‑1 (MQ=255)

           aaGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCttt                                                                              >  2:2914074/1‑93 (MQ=255)

           aaGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCttt                                                                              >  2:28050/1‑93 (MQ=255)

           aaGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCttt                                                                              >  2:2461707/1‑93 (MQ=255)

            aGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCtttt                                                                             <  2:1237379/93‑1 (MQ=255)

             ggTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCttttt                                                                            <  2:336954/93‑1 (MQ=255)

              gTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCtttttt                                                                           <  1:6095185/93‑1 (MQ=255)

               tGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTc                                                                          >  2:2382239/1‑93 (MQ=255)

                 ccGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTccg                                                                        >  2:5361309/1‑93 (MQ=255)

                 ccGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTccg                                                                        <  2:6042856/93‑1 (MQ=255)

                   gagaATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTccgcc                                                                      >  2:4872408/1‑93 (MQ=255)

                   gagaATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTccgcc                                                                      >  1:3842707/1‑93 (MQ=255)

                     gaATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTccgccgc                                                                    <  1:1259764/93‑1 (MQ=255)

                      aaTATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTccgccgcc                                                                   >  2:3649650/1‑93 (MQ=255)

                      aaTATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTccgccgcc                                                                   <  1:3672487/93‑1 (MQ=255)

                       atatCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTccgccgccg                                                                  <  2:5930586/93‑1 (MQ=255)

                         atCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAg                                                                <  1:1376058/93‑1 (MQ=255)

                          tctcGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAgc                                                               <  2:5548915/93‑1 (MQ=255)

                            tcGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAgcgc                                                             >  2:3357344/1‑93 (MQ=255)

                            tcGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAgcgc                                                             <  1:5462391/93‑1 (MQ=255)

                            tcGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAgcgc                                                             >  1:5906286/1‑93 (MQ=255)

                              gcaggcagAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCgg                                                           <  2:3146940/93‑1 (MQ=255)

                              gcaggcagAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCgg                                                           >  2:1661962/1‑93 (MQ=255)

                              gcaggcagAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCgg                                                           <  2:1772737/93‑1 (MQ=255)

                                aggcagAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGGCGAGCGCGGGc                                                         >  1:2646271/1‑93 (MQ=255)

                                aggcagAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGc                                                         >  1:686646/1‑93 (MQ=255)

                                 ggcagAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCg                                                        >  1:3713028/1‑93 (MQ=255)

                                 ggcagAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCg                                                        >  1:485717/1‑93 (MQ=255)

                                   cagAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGtt                                                      <  1:5951569/93‑1 (MQ=255)

                                     gagCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCa                                                    >  1:3264515/1‑93 (MQ=255)

                                     gagCAGGCTAAAGTGGGCGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGGTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCa                                                    >  1:5654382/1‑93 (MQ=255)

                                      agCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCAt                                                   <  2:2259462/93‑1 (MQ=255)

                                      agCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCAt                                                   <  2:5035630/93‑1 (MQ=255)

                                       gCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCAtt                                                  <  2:389764/93‑1 (MQ=255)

                                       gCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCAtt                                                  >  1:1298944/1‑93 (MQ=255)

                                       gCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGGTCTTTTTTCGGCCGCGAGGGGGGGGGGTTCAtt                                                  >  1:3327680/1‑93 (MQ=255)

                                          ggCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAg                                               >  2:300579/1‑93 (MQ=255)

                                            cTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGcc                                             <  2:66684/93‑1 (MQ=255)

                                            cTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGcc                                             <  1:3144950/93‑1 (MQ=255)

                                            cTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGcc                                             >  2:5005694/1‑93 (MQ=255)

                                            cTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGcc                                             <  2:1010072/93‑1 (MQ=255)

                                             tAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCt                                            <  2:1961541/93‑1 (MQ=255)

                                             tAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCt                                            <  2:2654133/93‑1 (MQ=255)

                                             tAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCt                                            <  1:4559045/93‑1 (MQ=255)

                                             tAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCt                                            <  2:6106864/93‑1 (MQ=255)

                                             tAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCt                                            <  2:3836910/93‑1 (MQ=255)

                                               aaGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGc                                          >  1:5296515/1‑93 (MQ=255)

                                                aGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCt                                         <  2:3289371/93‑1 (MQ=255)

                                                 gTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTc                                        >  2:1145805/1‑93 (MQ=255)

                                                 gTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTc                                        >  2:5199408/1‑93 (MQ=255)

                                                 gTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTc                                        >  1:896816/1‑93 (MQ=255)

                                                  tGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTcc                                       <  2:6015646/93‑1 (MQ=255)

                                                  tGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTcc                                       <  2:1711411/93‑1 (MQ=255)

                                                  tGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTcc                                       <  2:4084553/93‑1 (MQ=255)

                                                  tGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGAGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTcc                                       >  1:2553283/1‑93 (MQ=255)

                                                   gggTGTTGTGTATGCTGAGTATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTccc                                      >  2:4356018/1‑93 (MQ=255)

                                                   gggTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTccc                                      <  1:1056641/93‑1 (MQ=255)

                                                   gggTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTccc                                      >  2:921644/1‑93 (MQ=255)

                                                    ggTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCg                                     <  1:4275059/93‑1 (MQ=255)

                                                     gtgtTGTGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCgg                                    <  1:287435/93‑1 (MQ=255)

                                                      ggttgtGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGa                                   <  1:5024339/92‑1 (MQ=255)

                                                       gttgtGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGaa                                  >  1:1797375/1‑93 (MQ=255)

                                                        ttgtGTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAat                                 <  2:5662783/93‑1 (MQ=255)

                                                         tgtgTATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAata                                <  2:3512319/93‑1 (MQ=255)

                                                          gtgtATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCTGAatat                               >  2:2859131/1‑93 (MQ=255)

                                                          gtgtATGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAatat                               >  2:2761570/1‑93 (MQ=255)

                                                              aTGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATtctc                           >  1:403426/1‑93 (MQ=255)

                                                              aTGCTGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATtctc                           <  2:1376852/93‑1 (MQ=255)

                                                                gCTGAATATGGTTTAACCGAATCGGGGGTCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCtt                         <  1:6186058/93‑1 (MQ=255)

                                                                  tGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCtttt                       <  2:533535/93‑1 (MQ=255)

                                                                  tGAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCtttt                       >  2:5907457/1‑93 (MQ=255)

                                                                   gAATATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCttttt                      <  1:1557131/93‑1 (MQ=255)

                                                                    aaTATGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTa                     >  1:6327870/1‑93 (MQ=255)

                                                                      tatGGTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATc                   <  1:2754822/93‑1 (MQ=255)

                                                                          gTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTGc               >  2:2101539/1‑93 (MQ=255)

                                                                          gTTTAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTGc               >  2:396778/1‑93 (MQ=255)

                                                                            ttAACCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGt             <  1:348050/93‑1 (MQ=255)

                                                                               aCCGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTaaa          <  2:3744831/93‑1 (MQ=255)

                                                                                ccGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTaaaa         <  2:5150495/93‑1 (MQ=255)

                                                                                ccGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTaaaa         >  1:397664/1‑93 (MQ=255)

                                                                                ccGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTaaaa         <  1:4385026/93‑1 (MQ=255)

                                                                                 cGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTTATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAg        >  1:3337655/1‑93 (MQ=255)

                                                                                 cGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAg        >  2:1088074/1‑93 (MQ=255)

                                                                                 cGAATCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGGTCATTGAGCCTGCTCCCGGAATATTTTCTTTTTATTCTGCGTAAAAg        >  1:2258770/1‑93 (MQ=255)

                                                                                   aaTCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCa      >  2:814856/1‑93 (MQ=255)

                                                                                    aTCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCAc     <  1:1354520/93‑1 (MQ=255)

                                                                                     tCGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGGTCATTGAGCCTGCTCCCCGAATATTCTCTTTTTATTCTGCGTAAAAGCACt    >  1:2279996/1‑93 (MQ=255)

                                                                                      cGGGGGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTa   <  2:6313457/93‑1 (MQ=255)

GGATCCGGCGAAAGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTAT  >  NC_000913/323317‑323496

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`