BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,304,497	A→G	R352R (CGT→CGC)	deaD ←	inducible ATP‑independent RNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,304,497	0	A	G	100.0%	969.9 / NA	284	R352R (CGT→CGC)	deaD	inducible ATP‑independent RNA helicase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (132/152); total (132/152)

Only 100 of 297 total aligned reads displayed.

CTTCATAGTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTACGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGGCCACGGGC  >  NC_000913/3304407‑3304586

 ttCATAGTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGa                                                                                        >  2:6216437/1‑93 (MQ=255)

 ttCATAGTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGa                                                                                        >  2:3364157/1‑93 (MQ=255)

 ttCATAGTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGa                                                                                        <  1:2366373/93‑1 (MQ=255)

  tCATAGTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGAc                                                                                       <  1:368465/93‑1 (MQ=255)

    aTAGTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCg                                                                                     >  1:1001842/1‑93 (MQ=255)

    aTAGTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCg                                                                                     >  2:3888/1‑93 (MQ=255)

      aGTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGAGCGGCCAGCACGACCCGCACGACCGGTGCGACCGAt                                                                                   >  2:1786630/1‑93 (MQ=255)

       gTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATa                                                                                  >  2:867178/1‑93 (MQ=255)

       gTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATa                                                                                  <  1:4600780/93‑1 (MQ=255)

        tACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATAc                                                                                 <  1:881145/93‑1 (MQ=255)

        tACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATAc                                                                                 <  1:3091273/93‑1 (MQ=255)

         aCGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACg                                                                                >  1:5532975/1‑93 (MQ=255)

           gTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGt                                                                              >  2:4336002/1‑93 (MQ=255)

            ttCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGAACCGCACGACCGGTGCGACCGATACgggg                                                                             >  1:3455066/1‑91 (MQ=255)

             tCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGa                                                                            <  1:5631072/93‑1 (MQ=255)

             tCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGa                                                                            <  2:3221289/93‑1 (MQ=255)

              cAATGTTGCGCAGGAGACGACGCTCGCGGTTCTCACCGAACAGGAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGaa                                                                           <  1:4491386/93‑1 (MQ=255)

              cAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGaa                                                                           >  1:5103660/1‑93 (MQ=255)

              cAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGaa                                                                           <  1:1480078/93‑1 (MQ=255)

               aaTGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAAc                                                                          >  1:2520088/1‑93 (MQ=255)

                aTGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACg                                                                         >  2:6094129/1‑93 (MQ=255)

                  gTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTa                                                                       >  2:2319493/1‑93 (MQ=255)

                     gcgcAGCAGACGACGCTCGCGGTTCTCCACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGa                                                                    >  1:3133468/1‑93 (MQ=255)

                     gcgcAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGa                                                                    >  1:3242696/1‑93 (MQ=255)

                     gcgcAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGa                                                                    <  1:5198429/93‑1 (MQ=255)

                      cgcAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGAc                                                                   <  2:6330311/93‑1 (MQ=255)

                      cgcAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGAc                                                                   <  1:6435407/93‑1 (MQ=255)

                      cgcAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGAc                                                                   <  1:6319578/93‑1 (MQ=255)

                       gcagcaGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACt                                                                  >  2:2050742/1‑93 (MQ=255)

                       gcagcaGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACt                                                                  >  1:1690002/1‑93 (MQ=255)

                       gcagcaGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACt                                                                  >  2:1711419/1‑93 (MQ=255)

                       gcagcaGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACt                                                                  >  1:5686939/1‑93 (MQ=255)

                        cagcagACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTc                                                                 >  2:4865826/1‑93 (MQ=255)

                         agcagACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCa                                                                <  1:4155654/93‑1 (MQ=255)

                          gcagACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAg                                                               >  2:4067695/1‑93 (MQ=255)

                            agACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGaa                                                             >  2:5874913/1‑93 (MQ=255)

                             gacgacGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAAt                                                            >  1:6161111/1‑93 (MQ=255)

                             gacgacGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAAt                                                            >  2:815322/1‑93 (MQ=255)

                             gacgacGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAAt                                                            >  2:5465470/1‑93 (MQ=255)

                             gacgacGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAAt                                                            <  1:825611/93‑1 (MQ=255)

                             gacgacGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAAt                                                            >  1:1254297/1‑93 (MQ=255)

                              acgacgCTCGCGGTTCTCAACGAACAGCAGCGCGCGGGCAGCACGACCCGCACGACCGGTGCGCCCGATACGGTGAAAGCAAGACTCAGAATc                                                           >  2:3317797/1‑93 (MQ=255)

                              acgacgCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATc                                                           <  2:3178403/93‑1 (MQ=255)

                              acgacgCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATc                                                           <  1:667648/93‑1 (MQ=255)

                               cgacgCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATcc                                                          >  1:1410297/1‑93 (MQ=255)

                               cgacgCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATcc                                                          <  1:1940310/93‑1 (MQ=255)

                               cgacgCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATcc                                                          <  1:2801858/93‑1 (MQ=255)

                                gacgCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCa                                                         >  1:4078459/1‑93 (MQ=255)

                                gacgCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCa                                                         <  2:1857751/93‑1 (MQ=255)

                                 acgCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCAt                                                        >  2:1576506/1‑93 (MQ=255)

                                  cgCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATc                                                       <  1:4354238/93‑1 (MQ=255)

                                   gCTCGCTTTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCg                                                      <  1:482046/93‑1 (MQ=255)

                                   gCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCg                                                      >  2:4626920/1‑93 (MQ=255)

                                   gCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCg                                                      <  1:3374238/93‑1 (MQ=255)

                                   gCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCg                                                      >  2:2671415/1‑93 (MQ=255)

                                   ccTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCg                                                      <  2:1990663/92‑1 (MQ=255)

                                      cgcgGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATGCGGTGAACGTAAGACTCAGAATCCATCGGGa                                                   <  2:4242620/93‑1 (MQ=255)

                                      cgcgGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGa                                                   >  2:779302/1‑93 (MQ=255)

                                        cgtttCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGata                                                 <  2:5547795/90‑1 (MQ=255)

                                         ggTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGGACGTAAGACTCAGAATCCAGCGGGatag                                                >  2:1432385/1‑92 (MQ=255)

                                          gTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATc                                               <  2:3607856/93‑1 (MQ=255)

                                           ttCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCg                                              >  1:2299451/1‑93 (MQ=255)

                                            tctcAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGt                                             <  1:5847864/93‑1 (MQ=255)

                                             ctcAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTa                                            >  1:1019819/1‑93 (MQ=255)

                                             ctcAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTa                                            >  2:1645677/1‑93 (MQ=255)

                                               cAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGt                                          <  2:5729106/93‑1 (MQ=255)

                                               cAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGt                                          >  1:5476976/1‑93 (MQ=255)

                                                 aCGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTa                                        >  2:2388859/1‑93 (MQ=255)

                                                 aCGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTa                                        >  2:5202064/1‑93 (MQ=255)

                                                   gAACAGCATCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAAc                                      <  1:2735814/93‑1 (MQ=255)

                                                    aaCAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACt                                     >  1:4950406/1‑93 (MQ=255)

                                                    aaCAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACt                                     >  1:3983963/1‑93 (MQ=255)

                                                     aCAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTa                                    >  2:4017422/1‑93 (MQ=255)

                                                      cagcagCGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTAc                                   >  2:5604205/1‑93 (MQ=255)

                                                       agcagcGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTAcc                                  >  1:3020204/1‑93 (MQ=255)

                                                        gcagcGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCa                                 >  1:2015162/1‑93 (MQ=255)

                                                        gcagcGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCa                                 <  2:5264264/93‑1 (MQ=255)

                                                        gcagcGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCa                                 <  1:2188353/93‑1 (MQ=255)

                                                         cagcGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAATTACCAg                                <  1:5362437/93‑1 (MQ=255)

                                                         cagcGCGCGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAg                                >  1:4206364/1‑93 (MQ=255)

                                                           gcgcgcGGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGc                              >  2:5152341/1‑93 (MQ=255)

                                                             gcgcgGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTg                            <  2:1105645/93‑1 (MQ=255)

                                                              cgcgGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGa                           <  1:4417694/93‑1 (MQ=255)

                                                                cgGCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGAGTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATa                         <  2:4530241/93‑1 (MQ=255)

                                                                 ggCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATAc                        <  1:6027581/93‑1 (MQ=255)

                                                                 ggCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATAc                        <  2:2484713/93‑1 (MQ=255)

                                                                  gCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACg                       >  1:1152593/1‑93 (MQ=255)

                                                                  gCCAGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACg                       >  2:6452569/1‑93 (MQ=255)

                                                                     aGCACGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTc                    >  2:4099665/1‑93 (MQ=255)

                                                                         cGACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACg                <  2:4820639/93‑1 (MQ=255)

                                                                          gACCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGt               <  2:2838494/93‑1 (MQ=255)

                                                                           aCCCGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTc              <  2:1159299/93‑1 (MQ=255)

                                                                             ccGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCa            >  1:1794347/1‑93 (MQ=255)

                                                                             ccGCACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCa            <  1:5968346/93‑1 (MQ=255)

                                                                                cACGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGGc         <  1:3823913/93‑1 (MQ=255)

                                                                                  cGACCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGGCCa       >  1:552111/1‑93 (MQ=255)

                                                                                    aCCGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGGCCACg     >  2:1290291/1‑93 (MQ=255)

                                                                                     ccGGTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGGCCACgg    >  2:2207025/1‑93 (MQ=255)

                                                                                       ggTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCGACGTCCAGGCCACGGGc  >  1:4904623/1‑93 (MQ=255)

                                                                                       ggTGCGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGGCCACGGGc  <  2:3421995/93‑1 (MQ=255)

CTTCATAGTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGACCGGTACGACCGATACGGTGAACGTAAGACTCAGAATCCATCGGGATATCGTAGTTAACTACCAGGCTGATACGCTCAACGTCCAGGCCACGGGC  >  NC_000913/3304407‑3304586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`