BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,345,093	A→G	E113G (GAA→GGA)	yhbJ →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,345,093	0	A	G	99.6%	847.1 / NA	246	E113G (GAA→GGA)	yhbJ	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (125/119); total (125/121)
Fisher's exact test for biased strand distribution p-value = 4.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 254 total aligned reads displayed.

TGTTCCTGGATGCCGACCGTAATACCTTAATTCGTCGTTACAGTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGAAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCGATACCTCAGAAATGTCCGTTCAC  >  NC_000913/3345003‑3345181

tGTTCCTGGATGCCGACCGTAATACCTTAATTCGTCGTTACAGTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGgaa                                                                                        >  2:186494/1‑93 (MQ=255)

tGTTCCTGGATGCCGACCGTAATACCTTAATTCGTCGTTACAGTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGgaa                                                                                        <  1:367773/93‑1 (MQ=255)

tGTTCCTGGATGCCGACCGTAATACCTTAATTCGTCGTTACAGTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGgaa                                                                                        <  2:4254800/93‑1 (MQ=255)

   tCCTGGATGCCGACCGTAATACCTTAATTCGTCGTTACAGTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTg                                                                                     >  2:3100431/1‑93 (MQ=255)

     cTGGATGCCGACCGTAATACCTTAATTCGTCGTTACAGTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCt                                                                                   >  2:6109495/1‑93 (MQ=255)

       ggATGCCGACCGTAATACCTTAATTCGTCGTTACAGTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTAt                                                                                 <  1:5368119/93‑1 (MQ=255)

        gATGCCGACCGTAATACCTTAATTCGTCGTTACAGTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATc                                                                                >  2:6140725/1‑93 (MQ=255)

        gATGCCGACCGTAATACCTTAATTCGTCGTTACAGTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATc                                                                                <  1:1655001/93‑1 (MQ=255)

          tGCCGACCGTAATACCTTAATTCGTCGTTACAGTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGa                                                                              <  2:5915808/93‑1 (MQ=255)

          tGCCGACCGTAATACCTTAATTCGTCGTTACAGTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGa                                                                              >  1:2275994/1‑93 (MQ=255)

            ccGACCGTAATACCTTAATTCGTCGTTACAGTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACa                                                                            >  2:4688469/1‑93 (MQ=255)

             cGACCGTAATACCTTAATTCGTCGTTACAGTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACaa                                                                           >  1:6442129/1‑93 (MQ=255)

              gACCGTAATACCTTAATTCGTCGTTACAGTGACACGCGCCGACTGCATCCTCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACaaa                                                                          <  2:4534859/93‑1 (MQ=255)

              gACCGTAATACCTTAATTCGTCGTTACAGTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACaaa                                                                          <  1:391336/93‑1 (MQ=255)

                  ggaaTACCTTAATTCGTCGTTACAGTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACaaagaaa                                                                      >  1:6383618/3‑93 (MQ=255)

                  gTAATACCTTAATTCGTCGTTACAGTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACaaagaaa                                                                      >  1:5015386/1‑93 (MQ=255)

                   tAATACCTTAATTCGTCGTTACAGTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACaaagaaag                                                                     >  2:4572719/1‑93 (MQ=255)

                     aTACCTTAATTCGTCGTTACAGTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCg                                                                   >  1:1176300/1‑93 (MQ=255)

                      tACCTTAATTCGTCGTTACAGTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGa                                                                  <  2:5645775/93‑1 (MQ=255)

                      tACCTTAATTCGTCGTTACAGTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGa                                                                  >  2:6444289/1‑93 (MQ=255)

                       aCCTTAATTCGTCGTTACAGTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGAt                                                                 >  2:4185850/1‑93 (MQ=255)

                       aCCTTAATTCGTCGTTACAGTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGAt                                                                 >  1:3112623/1‑93 (MQ=255)

                         cTTAATTCGTCGTTACAGTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGAttt                                                               >  1:1014893/1‑93 (MQ=255)

                          ttAATTCGTCGTTACAGTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTg                                                              <  2:6244753/93‑1 (MQ=255)

                             aTTCGTCGTTACAGTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTg                                                           >  2:2863212/1‑93 (MQ=255)

                             aTTCGTCGTTACAGTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTg                                                           <  2:3434405/93‑1 (MQ=255)

                               tcgtcgTTACAGTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGa                                                         <  2:1585295/93‑1 (MQ=255)

                                cgtcgtTACAGTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAg                                                        >  2:1055889/1‑93 (MQ=255)

                                  tcgtTACATTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGcc                                                      <  1:4794488/93‑1 (MQ=255)

                                  tcgtTACAGTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGcc                                                      <  1:3401535/93‑1 (MQ=255)

                                  tcgtTACAGTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGcc                                                      <  2:2723231/93‑1 (MQ=255)

                                  tcgtTACAGTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGcc                                                      <  1:1759280/93‑1 (MQ=255)

                                  tcgtTACAGTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGcc                                                      <  1:2154444/93‑1 (MQ=255)

                                    gtTACAGTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCctc                                                    >  2:582778/1‑93 (MQ=255)

                                    gtTACAGTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGAGTTGCTGGAGCctc                                                    >  2:2538258/1‑93 (MQ=255)

                                     ttACAGTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCctct                                                   <  2:3415092/93‑1 (MQ=255)

                                     ttACAGTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCctct                                                   <  1:2744792/93‑1 (MQ=255)

                                     ttACAGTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCctct                                                   >  2:2560160/1‑93 (MQ=255)

                                      tACAGTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTg                                                  >  1:5407414/1‑93 (MQ=255)

                                      tACAGTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTg                                                  <  2:3964657/93‑1 (MQ=255)

                                      tACAGTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTg                                                  <  2:4465522/93‑1 (MQ=255)

                                      tACAGTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGCAGCCTCTg                                                  >  1:1369989/1‑93 (MQ=255)

                                       aCAGTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGc                                                 >  2:3290748/1‑93 (MQ=255)

                                       aCAGTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGc                                                 <  2:2991336/93‑1 (MQ=255)

                                        cAGTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCg                                                >  2:2465589/1‑93 (MQ=255)

                                          gTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGTCTCTGCGtt                                              >  1:6137050/1‑93 (MQ=255)

                                          gTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGtt                                              >  2:133501/1‑93 (MQ=255)

                                          gTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGtt                                              >  1:6195619/1‑93 (MQ=255)

                                          gTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGtt                                              <  2:546274/93‑1 (MQ=255)

                                               acGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGa                                         >  1:2998206/1‑93 (MQ=255)

                                                  cgcCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCg                                      <  1:755862/93‑1 (MQ=255)

                                                   gcCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCgg                                     <  1:4969105/93‑1 (MQ=255)

                                                      gACTGCATCCGCTTTCCAGCAAAACCCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATc                                  <  1:5282755/93‑1 (MQ=255)

                                                      gACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATc                                  <  1:2467954/93‑1 (MQ=255)

                                                       aCTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCt                                 >  1:1024669/1‑93 (MQ=255)

                                                       aCTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCt                                 >  1:5573269/1‑93 (MQ=255)

                                                       aCTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCt                                 >  1:6358448/1‑93 (MQ=255)

                                                        cTGCATCCGCTTTCCAGCAAAAACCTTTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTg                                <  1:4484549/93‑1 (MQ=255)

                                                        cTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTg                                <  1:1980395/93‑1 (MQ=255)

                                                        cTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTg                                >  1:5197311/1‑93 (MQ=255)

                                                         tGCATCCGCTTTCCAGCAAAACCCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGa                               <  2:4722172/93‑1 (MQ=255)

                                                          gCATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGAt                              >  1:3984284/1‑93 (MQ=255)

                                                           cATCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGAtt                             >  1:5953324/1‑93 (MQ=255)

                                                            aTCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTg                            >  2:231175/1‑93 (MQ=255)

                                                            aTCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTg                            >  2:1404183/1‑93 (MQ=255)

                                                            aTCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTg                            <  1:2565681/93‑1 (MQ=255)

                                                             tCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGt                           >  2:3698464/1‑93 (MQ=255)

                                                             tCCGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGt                           >  1:1576833/1‑93 (MQ=255)

                                                               cGCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCg                         >  2:1732394/1‑93 (MQ=255)

                                                                gCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCGa                        >  1:1276382/1‑93 (MQ=255)

                                                                gCTTTCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTAGa                        >  2:5207901/1‑93 (MQ=255)

                                                                gCTTTCCAGCAAAAACCTGTCGCTGGAAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCGa                        <  2:6284641/93‑1 (MQ=255)

                                                                  tttCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCGATa                      >  2:4446794/1‑93 (MQ=255)

                                                                  tttCCAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCGATa                      >  1:1341990/1‑93 (MQ=255)

                                                                     ccAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCGATACCt                   <  1:2485878/93‑1 (MQ=255)

                                                                     ccAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCGATACCt                   <  2:6054419/93‑1 (MQ=255)

                                                                     ccAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCGATACCt                   <  2:1048979/93‑1 (MQ=255)

                                                                     ccAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCGATACCt                   >  2:1648346/1‑93 (MQ=255)

                                                                     ccAGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCGATACCt                   <  1:629546/93‑1 (MQ=255)

                                                                       aGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCGATACCTCa                 <  2:3910499/93‑1 (MQ=255)

                                                                       aGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCGATACCTCa                 >  2:210642/1‑93 (MQ=255)

                                                                       aGCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCGATACCTCa                 <  2:2132627/93‑1 (MQ=255)

                                                                        gCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCGATACCTCAg                >  1:5826632/1‑93 (MQ=255)

                                                                        gCAAAAACCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGAGGTTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCGATACCTCat                >  1:927087/1‑92 (MQ=255)

                                                                           aaaaCCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCGATACCTCAGaaa             <  2:5733371/93‑1 (MQ=255)

                                                                            aaaCCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCGATACCTCAGAAAt            >  1:6079524/1‑93 (MQ=255)

                                                                             aaCCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCGATACCTCAGAAATg           >  1:5059553/1‑93 (MQ=255)

                                                                              aCCTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCGATACCTCAGAAATGt          >  2:1760001/1‑93 (MQ=255)

                                                                               ccTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCGATACCTCAGAAATGTc         <  1:3578423/93‑1 (MQ=255)

                                                                               ccTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCGATACCTCAGAAATGTc         >  1:2707802/1‑93 (MQ=255)

                                                                                cTGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCGATACCTCAGAAATGTcc        >  1:4240170/1‑93 (MQ=255)

                                                                                 tGTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCGATACCTCAGAAATGTCCg       <  1:6477271/93‑1 (MQ=255)

                                                                                  gTCGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCGATACCTCAGAAATGTCCGt      >  1:218605/1‑93 (MQ=255)

                                                                                    cGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCGATACCTCAGAAATGTCCGTTc    <  2:5008694/93‑1 (MQ=255)

                                                                                    cGCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCGATACCTCAGAAATGTCCGTTc    <  2:4884941/93‑1 (MQ=255)

                                                                                     gCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCGATACCTCAGAAATGTCCGTTCa   <  1:37924/93‑1 (MQ=255)

                                                                                     gCTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCGATACCTCAGAAATGTCCGTTCa   >  1:4636752/1‑93 (MQ=255)

                                                                                      cTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCGATACCTCAGAAATGTCCGTTCAc  >  2:3260528/1‑93 (MQ=255)

                                                                                      cTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCGATACCTCAGAAATGTCCGTTCAc  <  1:3169349/93‑1 (MQ=255)

                                                                                      cTGGGAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCGATACCTCAGAAATGTCCGTTCAc  >  1:965419/1‑93 (MQ=255)

TGTTCCTGGATGCCGACCGTAATACCTTAATTCGTCGTTACAGTGACACGCGCCGACTGCATCCGCTTTCCAGCAAAAACCTGTCGCTGGAAAGTGCTATCGACAAAGAAAGCGATTTGCTGGAGCCTCTGCGTTCGCGAGCGGATCTGATTGTCGATACCTCAGAAATGTCCGTTCAC  >  NC_000913/3345003‑3345181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`