Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,358,623	A→G	intergenic (+372/‑188)	gltD → / → gltF	glutamate synthase small subunit/GltF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,358,623	0	A	G	100.0%	25.8 / NA	9	intergenic (+372/‑188)	gltD/gltF	glutamate synthase small subunit/GltF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/4);  total (5/4)

ATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTCATTAAATAAATACATCCCAGCAA  >  NC_000913/3358559‑3358704                                                                 |                                                                                  attatCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTGTCACCACAAACCACCTCACCAGCACCtt                                                       <  1:3078762/93‑1 (MQ=255)   tatCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTGTCACCACAAACCACCTCACCAGCACCttat                                                     >  2:1244732/1‑93 (MQ=255)        ttAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTGTCACCACAAACCACCTCACCAGCACCTTATTATTc                                                >  2:4399508/1‑93 (MQ=255)                cTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTGTCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATc                                        <  1:326481/93‑1 (MQ=255)                        tttCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTGTCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACtat                                <  1:6477199/93‑1 (MQ=255)                               tttttCAGCGCAAACATTCATGTAATGAATAATTGTCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTc                         >  2:5617677/1‑93 (MQ=255)                                           aaCATTCATGTAATGAATAATTGTCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTCATTAaataaata             <  2:4907331/93‑1 (MQ=255)                                               ttCATGTAATGAATAATTGTCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTCATTAAATAAATACATc         >  2:2532054/1‑93 (MQ=255)                                                      aaTGAATAATTGTCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTCATTAAATAAATACATCCCAGCaa  >  1:4258366/1‑93 (MQ=255)                                                                 |                                                                                  ATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTCATTAAATAAATACATCCCAGCAA  >  NC_000913/3358559‑3358704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑