BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,477,397	T→C	N283S (AAC→AGC)	kefB ←	KefB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,477,397	0	T	C	100.0%	904.4 / NA	258	N283S (AAC→AGC)	kefB	KefB
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (127/131); total (127/131)

Only 100 of 260 total aligned reads displayed.

GCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCA  >  NC_000913/3477307‑3477486

gcCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTg                                                                                         <  1:3605371/93‑1 (MQ=255)

gcCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTg                                                                                         >  1:5269791/1‑93 (MQ=255)

gcCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTg                                                                                         >  2:5784647/1‑93 (MQ=255)

gcCAGCAGATACAGCACGAGAATCTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTg                                                                                         <  1:6110609/93‑1 (MQ=255)

 ccAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGa                                                                                        >  2:3589409/1‑93 (MQ=255)

  cagcagATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAg                                                                                       <  1:1665235/93‑1 (MQ=255)

      agATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGAc                                                                                   >  2:2701440/1‑93 (MQ=255)

      agATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGAc                                                                                   >  1:6264910/1‑93 (MQ=255)

      agATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGAGAc                                                                                   >  2:4609321/1‑93 (MQ=255)

       gATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACa                                                                                  >  2:1999497/1‑93 (MQ=255)

       gATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACa                                                                                  <  2:2841928/93‑1 (MQ=255)

          aCAGCACGAGAATTTTCACTGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGc                                                                               >  1:5938277/1‑93 (MQ=255)

          aCAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGc                                                                               >  2:5535900/1‑93 (MQ=255)

          aCAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGc                                                                               >  1:3541297/1‑93 (MQ=255)

          aCAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGc                                                                               >  2:3988118/1‑93 (MQ=255)

           cAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGcc                                                                              <  2:4378733/93‑1 (MQ=255)

           cAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGcc                                                                              <  1:3520714/93‑1 (MQ=255)

            aGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCg                                                                             >  1:2300771/1‑93 (MQ=255)

             gCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGa                                                                            <  1:3697853/93‑1 (MQ=255)

             gCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGa                                                                            >  2:5060951/1‑93 (MQ=255)

               aCGAGAATTTTCACCGCCACCAGCACAGCCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGTCa                                                                          <  1:5470790/93‑1 (MQ=255)

               aCGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACa                                                                          <  2:5883777/93‑1 (MQ=255)

               aCGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACa                                                                          <  2:2044782/93‑1 (MQ=255)

               aCGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACa                                                                          >  1:4143645/1‑93 (MQ=255)

                 gagaATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACaga                                                                        >  2:6115571/1‑93 (MQ=255)

                  agaATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACagag                                                                       <  2:1283195/93‑1 (MQ=255)

                    aaTTTTCACCGCCACCAGCACAACCACGCTTATCACTGCCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGAt                                                                     <  2:5443082/93‑1 (MQ=255)

                      ttttCACCGCCACCAGCACAACCTCGCTTACCACTACCCTCAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATaa                                                                   <  2:4618920/93‑1 (MQ=255)

                      ttttCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATaa                                                                   >  2:896767/1‑93 (MQ=255)

                       tttCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATaaa                                                                  <  2:5683757/93‑1 (MQ=255)

                          cACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGaa                                                               >  1:1565457/1‑93 (MQ=255)

                           aCCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAAc                                                              <  2:748508/93‑1 (MQ=255)

                           aCCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAAc                                                              >  1:5688102/1‑93 (MQ=255)

                            ccGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGAGATGCCGACAGAGATAAAGAACa                                                             >  2:1581543/1‑93 (MQ=255)

                            ccGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACa                                                             <  1:654827/93‑1 (MQ=255)

                            ccGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACa                                                             >  1:109751/1‑93 (MQ=255)

                              gCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACaaa                                                           <  2:3840594/93‑1 (MQ=255)

                                caccaGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGCCATGCCGACAGAGATAAAGAACAAAcc                                                         >  1:1881637/1‑93 (MQ=255)

                                  ccaGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGa                                                       <  2:1625096/93‑1 (MQ=255)

                                  ccaGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGa                                                       >  2:1010192/1‑93 (MQ=255)

                                  ccaGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGa                                                       >  2:3752167/1‑93 (MQ=255)

                                  ccaGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGa                                                       <  1:2063956/93‑1 (MQ=255)

                                    aGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGagc                                                     >  1:5871498/1‑93 (MQ=255)

                                     gCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGagca                                                    >  1:1634610/1‑93 (MQ=255)

                                      cacaACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAAACGagcag                                                   >  2:1734154/1‑93 (MQ=255)

                                       acaACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGagcagc                                                  >  1:2599399/1‑93 (MQ=255)

                                       acaACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGagcagc                                                  <  1:3038422/93‑1 (MQ=255)

                                        caACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAgcagca                                                 >  2:5599411/1‑93 (MQ=255)

                                         aaCCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAACCCGAGCAGCaa                                                >  1:4250129/1‑93 (MQ=255)

                                         aaCCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCaa                                                >  1:2207412/1‑93 (MQ=255)

                                         aaCCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCaa                                                >  2:6108425/1‑93 (MQ=255)

                                           ccACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGc                                              <  1:4422916/93‑1 (MQ=255)

                                           ccACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCGGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGc                                              <  1:4583224/93‑1 (MQ=255)

                                            cACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGcc                                             <  2:6365465/93‑1 (MQ=255)

                                             ccGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCt                                            <  2:1358815/92‑1 (MQ=255)

                                             ccGCTAATCACTACCCAGAACAGATGGCTATAAAGCACCCCGAGGCTGAGTGACATGCGGACAGAGATAAAGACCAAACCGAGCAGCAAGCCt                                            <  1:3157306/92‑1 (MQ=255)

                                               gCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCttt                                          <  1:4761831/93‑1 (MQ=255)

                                                cTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTg                                         <  2:4948459/93‑1 (MQ=255)

                                                  tATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGaa                                       <  1:4641095/93‑1 (MQ=255)

                                                   aTCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGCGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAg                                      >  1:797940/1‑93 (MQ=255)

                                                   aTCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGCAg                                      >  1:3316087/1‑93 (MQ=255)

                                                    tCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAgg                                     >  1:929654/1‑93 (MQ=255)

                                                    tCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAgg                                     >  1:5239112/1‑93 (MQ=255)

                                                    tCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAgg                                     <  2:2348123/93‑1 (MQ=255)

                                                     cACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAggg                                    <  2:2564893/93‑1 (MQ=255)

                                                     cACTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAggg                                    <  1:4911653/93‑1 (MQ=255)

                                                      aCTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGa                                   <  2:2418642/93‑1 (MQ=255)

                                                      aCTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGa                                   >  2:2070472/1‑93 (MQ=255)

                                                      aCTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGa                                   >  1:1731117/1‑93 (MQ=255)

                                                      aCTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGa                                   <  1:5512087/93‑1 (MQ=255)

                                                      aCTACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGa                                   >  1:6050229/1‑93 (MQ=255)

                                                        tACCCACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATc                                 >  1:4988881/1‑93 (MQ=255)

                                                         tcccACAAAAGAGGGGTATAAAGCACCCCGAGGCTGAGTGACATCCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCg                                <  1:1777840/92‑1 (MQ=255)

                                                          cccACAACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGa                               >  2:2630128/1‑93 (MQ=255)

                                                            cacaACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATa                             >  2:4512099/1‑93 (MQ=255)

                                                            cacaACAGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATa                             <  1:5801246/93‑1 (MQ=255)

                                                             acaacaGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAg                            <  1:6419302/93‑1 (MQ=255)

                                                              caacaGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGc                           <  2:305713/93‑1 (MQ=255)

                                                                acaGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCg                         >  2:4865106/1‑93 (MQ=255)

                                                                  aGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGtt                       <  1:4690847/93‑1 (MQ=255)

                                                                  aGATGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGtt                       <  1:5060951/93‑1 (MQ=255)

                                                                    aTGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTc                     <  2:2894130/93‑1 (MQ=255)

                                                                     tGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTcc                    <  2:3377142/93‑1 (MQ=255)

                                                                     tGGGTATAAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTcc                    >  1:2902030/1‑93 (MQ=255)

                                                                          ataAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTc               <  1:1295974/93‑1 (MQ=255)

                                                                          ataAAGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTc               >  2:4083886/1‑93 (MQ=255)

                                                                            aaaGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCAt             <  2:5218432/93‑1 (MQ=255)

                                                                             aaGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATg            >  1:278615/1‑93 (MQ=255)

                                                                              aGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATgg           >  1:1376455/1‑93 (MQ=255)

                                                                              aGCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATgg           >  1:366606/1‑93 (MQ=255)

                                                                              aGCAACCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATgg           <  1:5640924/93‑1 (MQ=255)

                                                                               gCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGc          <  2:3491545/93‑1 (MQ=255)

                                                                               gCACCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGc          <  2:1256982/93‑1 (MQ=255)

                                                                                 cccccGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGa        <  2:523618/92‑1 (MQ=255)

                                                                                 aCCCCGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGa        >  2:1861374/1‑93 (MQ=255)

                                                                                  ccccGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGat       <  1:117554/93‑1 (MQ=255)

                                                                                   cccGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGata      <  1:2428492/93‑1 (MQ=255)

                                                                                    ccGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGatat     >  2:3709820/1‑93 (MQ=255)

                                                                                     cGAGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATAtt    >  2:3178044/1‑93 (MQ=255)

                                                                                       aGGCTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGCAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCa  >  2:3333282/1‑93 (MQ=255)

GCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCA  >  NC_000913/3477307‑3477486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`