BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,677,270	T→C	T147T (ACT→ACC)	kdgK →	ketodeoxygluconokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,677,270	0	T	C	99.6%	866.8 / NA	249	T147T (ACT→ACC)	kdgK	ketodeoxygluconokinase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (117/131); minor base G (0/1); total (117/132)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 254 total aligned reads displayed.

GGCATTAACCGTTCATTACGTAACGGCGCTGGGAACGGACAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACTTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGACAGCACCGGCGAGCGTACGTTCTACTACTGGCG  >  NC_000913/3677180‑3677359

ggCATTAACCGTTCATTACGTAACGGCGCTGGGAACGGACAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTc                                                                                         <  1:5832003/93‑1 (MQ=255)

  cATTAACCGTTCATTACGTAACGGCGCTGGGAACGGACAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTccc                                                                                       <  1:1953494/93‑1 (MQ=255)

  acttAACCGTTCATTACGTAACGGCGCTGGGAACGGACAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATGCCTccc                                                                                       <  1:2009513/91‑1 (MQ=255)

   aTTAACCGTTCATTACGTAACGGCGCTGGGAACGGACAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTCCCt                                                                                      <  2:1579356/93‑1 (MQ=255)

   aTTAACCGTTCATTACGTAACGGCGCTGGGAACGGACAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTCCCt                                                                                      <  1:4428836/93‑1 (MQ=255)

        ccGTTCATTACGTAACGGCGCTGGGAACGGACAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGAccc                                                                                 >  1:1771411/1‑93 (MQ=255)

           ttCATTACGTAACGGCGCTGGGAACGGACAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAAc                                                                              >  2:6390525/1‑93 (MQ=255)

             cATTACGTAACGGCGCTGGGAACGGACAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACgg                                                                            <  1:3033361/93‑1 (MQ=255)

             cATTACGTAACGGCGCTGGGAACGGACAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACgg                                                                            <  2:6239613/93‑1 (MQ=255)

              aTTACGTAACGGCGCTGGGAACGGACAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGa                                                                           <  1:4192909/93‑1 (MQ=255)

               ttACGTAACGGCGCTGGGAACGGACAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGAt                                                                          <  1:3175694/93‑1 (MQ=255)

               ttACGTAACGGCGCTGGGAACGGACAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGAt                                                                          <  2:6479371/93‑1 (MQ=255)

               ttACGTAACGGCGCTGGGAACGGACAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGAt                                                                          <  1:251669/93‑1 (MQ=255)

                    tAACGGCGCTGGGAACGGACAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGaaa                                                                     >  2:1026088/1‑93 (MQ=255)

                         gcgcTGGGAACGGACAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGt                                                                >  2:1939070/1‑93 (MQ=255)

                           gcTGGGAACGGACAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCt                                                              <  1:3960760/93‑1 (MQ=255)

                           gcTGGGAACGGACAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCt                                                              <  2:2782698/93‑1 (MQ=255)

                            cTGGGAACGGACAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTg                                                             <  2:883597/93‑1 (MQ=255)

                            cTGGGAACGGACAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGCTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTg                                                             >  2:150444/1‑93 (MQ=255)

                             tGGGAACGGACAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGc                                                            >  1:3568921/1‑93 (MQ=255)

                             tGGGAACGGACAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGc                                                            >  2:761333/1‑93 (MQ=255)

                              gggAACGGACAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGcc                                                           >  1:5056415/1‑93 (MQ=255)

                              gggAACGGACAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGcc                                                           >  1:6176261/1‑93 (MQ=255)

                               ggAACGGACAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCg                                                          >  2:2922678/1‑93 (MQ=255)

                               ggAACGGACAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCg                                                          <  1:1498642/93‑1 (MQ=255)

                                gAACGGACAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCgg                                                         >  2:4028727/1‑93 (MQ=255)

                                  aCGGACAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGc                                                       >  2:3543304/1‑93 (MQ=255)

                                   cGGACAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGcc                                                      <  1:4987352/93‑1 (MQ=255)

                                    ggACAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCt                                                     >  1:881600/1‑93 (MQ=255)

                                    ggACAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCt                                                     >  1:5921312/1‑93 (MQ=255)

                                    ggACAGGTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCt                                                     >  2:5975134/1‑93 (MQ=255)

                                     gACAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCtt                                                    >  2:1479791/1‑93 (MQ=255)

                                      aCAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCttt                                                   >  1:2644055/1‑93 (MQ=255)

                                      aCAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCttt                                                   <  1:1398210/93‑1 (MQ=255)

                                       cAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTta                                                  >  2:4177572/1‑93 (MQ=255)

                                       cAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTta                                                  >  2:736328/1‑93 (MQ=255)

                                         gTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTtact                                                >  1:101791/1‑93 (MQ=255)

                                          ttttAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTtacta                                               >  2:4738623/1‑93 (MQ=255)

                                           tttAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTtactac                                              <  1:4068260/93‑1 (MQ=255)

                                           tttAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTtactac                                              <  2:556616/93‑1 (MQ=255)

                                           tttAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTtactac                                              >  1:4677946/1‑93 (MQ=255)

                                           tttAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTtactac                                              >  1:2272856/1‑93 (MQ=255)

                                             tAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACAt                                            >  2:6046869/1‑93 (MQ=255)

                                              aGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACAtt                                           <  2:5101122/93‑1 (MQ=255)

                                              aGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACAtt                                           >  2:4774636/1‑93 (MQ=255)

                                              aGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACAtt                                           >  1:4483786/1‑93 (MQ=255)

                                               gCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTg                                          >  1:334746/1‑93 (MQ=255)

                                                 cagcagATGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGaa                                        >  2:1320794/1‑93 (MQ=255)

                                                    cagATGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAAcc                                     <  1:5129979/93‑1 (MQ=255)

                                                      gATGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGa                                   >  1:571907/1‑93 (MQ=255)

                                                      gATGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGa                                   <  1:1260217/93‑1 (MQ=255)

                                                      gATGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGa                                   >  1:3119885/1‑93 (MQ=255)

                                                       aTGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGAc                                  <  1:4829056/93‑1 (MQ=255)

                                                       aTGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGAc                                  <  1:5340225/93‑1 (MQ=255)

                                                       aTGCTGGACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGAc                                  <  2:1159913/93‑1 (MQ=255)

                                                          cTGGACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGACAGc                               <  2:5622586/93‑1 (MQ=255)

                                                            ggACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGACAGCAc                             >  2:3105802/1‑93 (MQ=255)

                                                            ggACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGACAGCAc                             <  2:1145475/93‑1 (MQ=255)

                                                             gACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGACAGCAcc                            <  2:6191954/93‑1 (MQ=255)

                                                             gACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGACAGCAcc                            >  2:5175565/1‑93 (MQ=255)

                                                             gACGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGACAGCAcc                            >  2:2153805/1‑93 (MQ=255)

                                                              aCGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGACAGCACCg                           <  2:1648750/93‑1 (MQ=255)

                                                               cGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGACAGCACCgg                          >  2:4959124/1‑93 (MQ=255)

                                                               cGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGACAGCACCgg                          <  1:1174842/93‑1 (MQ=255)

                                                               cGCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGACAGCACCgg                          >  1:2449292/1‑93 (MQ=255)

                                                               cGCCTGGCACGGCAAGAACGTTGATACCTCCCTGACCCAACGGATGGACACCCGTCTGCCGGGCCTTTACTACATTGAAACCGACAGCACCgg                          <  1:6466323/93‑1 (MQ=255)

                                                                gCCTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGACAGCACCGGc                         <  1:5683662/93‑1 (MQ=255)

                                                                  cTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGACAGCACCGGCGa                       <  1:5192734/93‑1 (MQ=255)

                                                                  cTGGCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGACAGCACCGGCGa                       >  2:393962/1‑93 (MQ=255)

                                                                   cggCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGCAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGACAGCACCGGCGAg                      <  2:334815/92‑1 (MQ=255)

                                                                    ggCACGGCAAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGACAGCACCGGCGAGc                     >  1:6279532/1‑93 (MQ=255)

                                                                     gCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGACAGCACCGGCGAGCg                    >  2:3139515/1‑93 (MQ=255)

                                                                     gCACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGACAGCACCGGCGAGCg                    <  2:4613388/93‑1 (MQ=255)

                                                                      cACGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACTGACAGCACCGGCGGGCGt                   >  1:3631711/1‑93 (MQ=255)

                                                                       aCGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGACAGCACCGGCGAGCGTa                  >  1:6367605/1‑93 (MQ=255)

                                                                       aCGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGACAGCACCGGCGAGCGTa                  <  1:459740/93‑1 (MQ=255)

                                                                       aCGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGACAGCACCGGCGAGCGTa                  <  2:4593177/93‑1 (MQ=255)

                                                                       aCGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGACAGCACCGGCGAGCGTa                  <  2:4454185/93‑1 (MQ=255)

                                                                       aCGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGACAGCACCGGCGAGCGTa                  >  2:4309983/1‑93 (MQ=255)

                                                                       aCGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGACAGCACCGGCGAGCGTa                  <  2:377433/93‑1 (MQ=255)

                                                                       aCGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGACAGCACCGGCGAGCGTa                  <  1:3729170/93‑1 (MQ=255)

                                                                       aCGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGACAGCACCGGCGAGCGTa                  <  1:719290/93‑1 (MQ=255)

                                                                       aCGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGACAGCACCGGCGAGCGTa                  >  1:1619515/1‑93 (MQ=255)

                                                                        cGGCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGACAGCACCGGCGAGCGTAc                 <  1:5419322/93‑1 (MQ=255)

                                                                         ggCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGACAGCACCGGCGAGCGTACg                >  2:4422922/1‑93 (MQ=255)

                                                                         ggCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGACAGCACCGGCGAGCGTACg                <  1:3825207/93‑1 (MQ=255)

                                                                          gCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGACAGCACCGGCGAGCGTACGt               >  1:4543647/1‑93 (MQ=255)

                                                                          gCGAGAACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGACAGCACCGGCGAGCGTACGt               >  1:1214707/1‑93 (MQ=255)

                                                                            gagaACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGACAGCACCGGCGAGCGTACGTTc             <  1:4870310/93‑1 (MQ=255)

                                                                            gagaACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGACAGCACCGGCGAGCGTACGTTc             <  2:3631977/93‑1 (MQ=255)

                                                                            gagaACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGACAGCACCGGCGAGCGTACGTTc             <  1:2730990/93‑1 (MQ=255)

                                                                            gagaACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGACAGCACCGGCGAGCGTACGTTc             <  2:1871829/93‑1 (MQ=255)

                                                                             agaACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGACAGCACCGGCGAGCGTACGTTct            >  1:2151944/1‑93 (MQ=255)

                                                                              gaACGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGACAGCACCGGCGAGCGTACGTTcta           >  1:1864562/1‑93 (MQ=255)

                                                                                aCGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGACAGCACCGGCGAGCGTACGTTctact         >  2:3497180/1‑93 (MQ=255)

                                                                                aCGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGACAGCACCGGCGAGCGTACGTTctact         >  2:3202268/1‑93 (MQ=255)

                                                                                aCGTTGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGACAGCACCGGCGAGCGTACGTTctact         <  2:2400359/93‑1 (MQ=255)

                                                                                    tGATACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGACAGCACCGGCGAGCGTACGTTCTACTACTg     >  2:4232676/1‑93 (MQ=255)

                                                                                      aTACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGACAGCACCGGCGAGCGTACGTTCTACTACTGGc   <  1:1355152/93‑1 (MQ=255)

                                                                                      aTACCTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGACAGCACCGGCGAGCGTACGTTCTACTACTGGc   <  2:256702/93‑1 (MQ=255)

GGCATTAACCGTTCATTACGTAACGGCGCTGGGAACGGACAGTTTTAGCCAGCAGATGCTGGACGCCTGGCACGGCGAGAACGTTGATACTTCCCTGACCCAACGGATGGAAAACCGTCTGCCGGGCCTTTACTACATTGAAACCGACAGCACCGGCGAGCGTACGTTCTACTACTGGCG  >  NC_000913/3677180‑3677359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`