BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,745,459	T→C	L249P (CTC→CCC)	lyxK →	L‑xylulose kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,745,459	0	T	C	99.6%	843.7 / ‑4.6	247	L249P (CTC→CCC)	lyxK	L‑xylulose kinase
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (132/114); total (133/114)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.01e-01

Only 100 of 257 total aligned reads displayed.

GGGAGATCACCGCTCAGACAGCCGCCCTGACCGGTCTGAAAGCGGGTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACTCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGATGGGGACCTGGGCGGTGACCAGCGGCATAACCCGCGGTTTACGTGACG  >  NC_000913/3745369‑3745548

gggAGATCACCGCTCAGACAGCCGCCCTGACCGGTCTGAAAGCGGGTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCAccct                                                                                          >  1:5832938/1‑93 (MQ=255)

gggAGATCACCGCTCAGACAGCCGCCCTGACCGGTCTGAAAGCGGGTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCAccct                                                                                          >  1:254289/1‑93 (MQ=255)

gggAGATCACCGCTCAGACAGCCGCCCTGACCGGTCTGAAAGCGGGTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCAccct                                                                                          <  2:640960/93‑1 (MQ=255)

gggAGATCACCGCTCAGACAGCCGCCCTGACCGGTCTGAAAGCGGGTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCAccct                                                                                          <  1:1992387/93‑1 (MQ=255)

gggAGATCACCGCTCAGACAGCCGCCCTGACCGGTCTGAAAGCGGGTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCAccct                                                                                          <  1:5666839/93‑1 (MQ=255)

gggAGATCACCGCTCAGACAGCCGCCCTGACCGGTCTGAAAGCGGGTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCAccct                                                                                          <  2:3372752/93‑1 (MQ=255)

   agaTCACCGCTCAGACAGCCGCCCTGACCGGTCTGAAAGCGGGTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTgcg                                                                                       >  2:738095/1‑93 (MQ=255)

   agaTCACCGCTCAGACAGCCGCCCTGACCGGTCTGAAAGCGGGTACGCCCATTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTgcg                                                                                       >  2:811069/1‑93 (MQ=255)

     aTCACCGCTCAGACAGCCGCCCTGACCGGTCTGAAAGCGGGTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGcc                                                                                     >  1:2694372/1‑93 (MQ=255)

      tCACCGCTCAGACAGCCGCCCTGACCGGTCTGAAAGCGGGTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCg                                                                                    >  2:4978329/1‑93 (MQ=255)

       cACCGCTCAGACAGCCGCCCTGACCGGTCTGAAAGCGGGTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCgg                                                                                   >  2:359987/1‑93 (MQ=255)

       cACCGCTCAGACAGCCGCCCTGACCGGTCTGAAAGCGGGTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCgg                                                                                   >  2:249216/1‑93 (MQ=255)

       cACCGCTCAGACAGCCGCCCTGACCGGTCTGAAAGCGGGTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCgg                                                                                   >  1:2096538/1‑93 (MQ=255)

       cACCGCTCAGACAGCCGCCCTGACCGGTCTGAAAGCGGGTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCgg                                                                                   <  1:3307459/93‑1 (MQ=255)

        aCCGCTCAGACAGCCGCCCTGACCGGTCTGAAAGCGGGTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCggg                                                                                  >  2:6318413/1‑93 (MQ=255)

        aCCGCTCAGACAGCCGCCCTGACCGGTCTGAAAGCGGGTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCCCACCCTGCGCCggg                                                                                  >  2:1609995/1‑93 (MQ=255)

          cGCTCAGACAGCCGCCCTGACCGGTCTGAAAGCGGGTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGAt                                                                                <  1:5921970/93‑1 (MQ=255)

          cGCTCAGACAGCCGCCCTGACCGGTCTGAAAGCGGGTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGAt                                                                                <  2:609613/93‑1 (MQ=255)

          cGCTCAGACAGCCGCCCTGACCGGTCTGAAAGCGGGTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGAt                                                                                <  1:1244389/93‑1 (MQ=255)

            cTCAGACAGCCGCCCTGACCGGTCTGAAAGCGGGTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCg                                                                              <  2:4156/93‑1 (MQ=255)

             tCAGACAGCCGCCCTGACCGGTCTGAAAGCGGGTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGa                                                                             <  1:4522337/93‑1 (MQ=255)

             tCAGACAGCCGCCCTGACCGGTCTGAAAGCGGGTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGa                                                                             >  1:2506261/1‑93 (MQ=255)

               aGACAGCCGCCCTGACCGGTCTGAAAGCGGGTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAg                                                                           >  2:1441819/1‑93 (MQ=255)

               aGACAGCCGCCCTGACCGGTCTGAAAGCGGGTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAg                                                                           >  1:3079058/1‑93 (MQ=255)

                gACAGCCGCCCTGACCGGTCTGAAAGCGGGTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGa                                                                          <  1:1698163/93‑1 (MQ=255)

                gACAGCCGCCCTGACCGGTCTGAAAGCGGGTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGa                                                                          <  2:4720382/93‑1 (MQ=255)

                gACAGCCGCCCTGACCGGTCTGAAAGCGGGTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGa                                                                          <  1:2967292/93‑1 (MQ=255)

                 accgccgccCTGACCGGTCTGAAAGCGGGTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGAc                                                                         <  1:1845606/90‑1 (MQ=255)

                  cAGCCGCCCTGACCGGTCTGAAAGCGGGTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGACg                                                                        >  1:3938171/1‑93 (MQ=255)

                    gccgccCTGACCGGTCTGAAAGCGGGTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGACGaa                                                                      <  1:500374/93‑1 (MQ=255)

                      cgccCTGACCGGTCTGAAAGCGGGTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑tt                                                                   >  1:2229250/1‑93 (MQ=255)

                       gccCTGACCGGTCTGAAAGCGGGTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGCTCGAAGACGAA‑ttt                                                                  >  2:1824874/1‑93 (MQ=255)

                          cTGACCGGTCTGAAAGCGGGTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTAcc                                                               >  1:96159/1‑93 (MQ=255)

                          cTGACCGGTCTGAAAGCGGGTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTAcc                                                               >  1:5258360/1‑93 (MQ=255)

                          cTGACCGGTCTGAAAGCGGGTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTAcc                                                               <  2:1976541/93‑1 (MQ=255)

                           tGACCGGTCTGAAAGCGGGTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTAccc                                                              <  1:795720/93‑1 (MQ=255)

                             aCCGGTCTGAAAGCGGGTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTc                                                            >  2:6005292/1‑93 (MQ=255)

                             aCCGGTCTGAAAGCGGGTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTc                                                            <  1:3386765/93‑1 (MQ=255)

                              ccGGTCTGAAAGCGGGTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCa                                                           >  2:5137921/1‑93 (MQ=255)

                              ccGGTCTGAAAGCGGGTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCa                                                           <  1:632242/93‑1 (MQ=255)

                               cGGTCTGAAAGCGGGTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCaa                                                          >  2:2978495/1‑93 (MQ=255)

                                    tGAAAGCGGGTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCgg                                                     >  1:775930/1‑93 (MQ=255)

                                     gAAAGCGGGTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCATCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCggg                                                    >  1:3501385/1‑92 (MQ=255)

                                     gAAAGCGGGTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGt                                                    <  2:5861999/93‑1 (MQ=255)

                                      aaaGCGGGTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTg                                                   >  1:1758861/1‑93 (MQ=255)

                                      aaaGCGGGTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTg                                                   >  1:3765170/1‑93 (MQ=255)

                                      aaaGCGGGTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTg                                                   >  2:4992450/1‑93 (MQ=255)

                                       aaGCGGGTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGa                                                  >  2:4243991/1‑93 (MQ=255)

                                        aGCGGGTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGAt                                                 <  2:1177215/93‑1 (MQ=255)

                                        aGCGGGTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGAt                                                 <  1:3201787/93‑1 (MQ=255)

                                          cGGGTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGATgg                                               <  2:2400952/93‑1 (MQ=255)

                                             gTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGATggggc                                            >  1:609119/1‑92 (MQ=255)

                                             gTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGATGGGGa                                            <  1:3522666/93‑1 (MQ=255)

                                              tACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGATGGGGAc                                           >  1:1601509/1‑93 (MQ=255)

                                              tACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGATGGGGAc                                           <  2:4365873/93‑1 (MQ=255)

                                              tACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGATGGGGAc                                           >  1:3333255/1‑93 (MQ=255)

                                                cGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGATGGGGACCt                                         >  2:5904635/1‑93 (MQ=255)

                                                 gCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGATGGGGACCTg                                        <  2:4440513/93‑1 (MQ=255)

                                                 gCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGATGGGGACCTg                                        <  2:3724275/93‑1 (MQ=255)

                                                   ccGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGATGGGGACCTggg                                      >  1:1526869/1‑93 (MQ=255)

                                                   ccGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGATGGGGACCTggg                                      >  1:2854093/1‑93 (MQ=255)

                                                     gttgttGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGATGGGGACCTGGGCg                                    >  1:103614/1‑93 (MQ=255)

                                                     gttgttGGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGATGGGGACCTGGGCg                                    >  1:2190402/1‑93 (MQ=255)

                                                        gttgGCGGCCTGTTTGATGTGGTTTCCACCGCACTCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGATGGGGACCTGGGCGGTg                                 >  2:4454357/1‑93 (MQ=255)

                                                        gttgGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGATGGGGACCTGGGCGGTg                                 <  1:4717370/93‑1 (MQ=255)

                                                          tgGCGGCCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGATGGGGACCTGGGCGGTGAc                               <  2:4015560/93‑1 (MQ=255)

                                                           ggcggcCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGATGGGGACCTGGGCGGTGAcc                              >  2:626048/1‑93 (MQ=255)

                                                              ggcCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGATGGGGACCTGGGCGGTGACCAGc                           <  1:3632146/93‑1 (MQ=255)

                                                              ggcCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGATGGGGACCTGGGCGGTGACCAGc                           >  1:1285458/1‑93 (MQ=255)

                                                               gcCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGATGGGGACCTGGGCGGTGACCAGCg                          <  2:639038/93‑1 (MQ=255)

                                                               gcCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGATGGGGACCTGGGCGGTGACCAGCg                          <  2:4622586/93‑1 (MQ=255)

                                                               gcCTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGATGGGGACCTGGGCGGTGACCAGCg                          <  1:4024446/93‑1 (MQ=255)

                                                                 cTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGATGGGGACCTGGGCGGTGACCAGCGGc                        >  2:719730/1‑93 (MQ=255)

                                                                 cTGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGATGGGGACCTGGGCGGTGACCAGCGGc                        >  1:4272843/1‑93 (MQ=255)

                                                                  tGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGATGGGGACCTGGGCGGTGACCAGCGGCa                       >  1:2349785/1‑93 (MQ=255)

                                                                  tGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGATGGGGACCTGGGCGGTGACCAGCGGCa                       >  2:5829265/1‑93 (MQ=255)

                                                                  tGTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGATGGGGACCTGGGCGGTGACCAGCGGCa                       >  2:2653294/1‑93 (MQ=255)

                                                                   gTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGATGGGGACCTGGGCGGTGACCAGCGGCAt                      <  2:1307981/93‑1 (MQ=255)

                                                                   gTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGATGGGGACCTGGGCGGTGACCAGCGGCAt                      <  2:3195446/93‑1 (MQ=255)

                                                                   gTTTGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGATGGGGACCTGGGCGGTGACCAGCGGCAt                      >  2:5606999/1‑93 (MQ=255)

                                                                    tttGATGTGGTTTCCACCGCACCCTGCGCCGGGGTCGAAGAAGAATTTTCCCCCCAATGC‑GTGGTGGGGGGCCGGGGGGGGGCCCGGGGCATa                     >  1:5964032/1‑93 (MQ=255)

                                                                      tGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGATGGGGACCTGGGCGGTGACCAGCGGCATAAc                   <  1:643732/93‑1 (MQ=255)

                                                                      tGATGTGGTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGATGGGGACCTGGGCGGTGACCAGCGGCATAAc                   >  2:15284/1‑93 (MQ=255)

                                                                           tgGTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGATGGGGACCTGGGCGGTGACCAGCGGCATAACCcgcg              >  2:5497825/1‑93 (MQ=255)

                                                                           tgGTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGATGGGGACCTGGGCGGTGACCAGCGGCATAACCcgcg              >  2:2070017/1‑93 (MQ=255)

                                                                            ggTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGATGGGGACCTGGGCGGTGACCAGCGGCATAACCCGCgg             >  1:586513/1‑93 (MQ=255)

                                                                            ggTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGATGGGGACCTGGGCGGTGACCAGCGGCATAACCCGCgg             <  2:3927982/93‑1 (MQ=255)

                                                                             gTTTCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGATGGGGACCTGGGCGGTGACCAGCGGCATAACCCGCGGt            >  1:4916067/1‑93 (MQ=255)

                                                                               ttCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGATGGGGACCTGGGCGGTGACCAGCGGCATAACCCGCGGttt          >  1:6078914/1‑93 (MQ=255)

                                                                                tCCACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGATGGGGACCTGGGCGGTGACCAGCGGCATAACCCGCGGTTTa         >  1:44981/1‑93 (MQ=255)

                                                                                  cACCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGATGATGGGGACCTGGGCGGTGACCAGCGGCATAACCCGCGGTTTACg       >  1:5184190/1‑93 (MQ=255)

                                                                                   aCCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGATGGGGACCTGGGCGGTGACCAGCGGCATAACCCGCGGTTTACGt      >  1:6211649/1‑93 (MQ=255)

                                                                                   aCCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGATGGGGACCTGGGCGGTGACCAGCGGCATAACCCGCGGTTTACGt      <  1:5527890/93‑1 (MQ=255)

                                                                                   aCCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGATGGGGACCTGGGCGGTGACCAGCGGCATAACCCGCGGTTTACGt      <  1:1350870/93‑1 (MQ=255)

                                                                                   aCCGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGATGGGGACCTGGGCGGTGACCAGCGGCATAACCCGCGGTTTACGt      >  1:1118869/1‑93 (MQ=255)

                                                                                    ccGCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGATGGGGACCTGGGCGGTGACCAGCGGCATAACCCGCGGTTTACGTg     <  1:872452/93‑1 (MQ=255)

                                                                                      gCACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGATGGGGACCTGGGCGGTGCCCAGCGGCATAACCCGCGGTTTACGTGAc   <  1:2774860/93‑1 (MQ=255)

                                                                                       cACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGATGGGGACCTGGGCGGTGACCAGCGGCATAACCCGCGGTTTACGTGACg  >  2:379582/1‑93 (MQ=255)

                                                                                       cACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGATGGGGACCTGGGCGGTGACCAGCGGCATAACCCGCGGTTTACGTGACg  <  1:4383816/93‑1 (MQ=255)

                                                                                       cACCCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGATGGGGACCTGGGCGGTGACCAGCGGCATAACCCGCGGTTTACGTGACg  >  1:1423872/1‑93 (MQ=255)

GGGAGATCACCGCTCAGACAGCCGCCCTGACCGGTCTGAAAGCGGGTACGCCCGTTGTTGGCGGCCTGTTTGATGTGGTTTCCACCGCACTCTGCGCCGGGATCGAAGACGAA‑TTTACCCTCAATGCGGTGATGGGGACCTGGGCGGTGACCAGCGGCATAACCCGCGGTTTACGTGACG  >  NC_000913/3745369‑3745548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`