Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 440,417 C→T A51T (GCC→ACC)  xseB ← exonuclease VII small subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913440,4170CT99.5% 697.6 / ‑5.1 185A51T (GCC→ACC) xseBexonuclease VII small subunit
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (89/95);  total (89/96)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.02e-01

Only 100 of 189 total aligned reads displayed.

CTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACGGGTTACAA  >  NC_000913/440328‑440506
                                                                                         |                                                                                         
cTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTg                                                                                        >  1:4070242/1‑93 (MQ=255)
  cATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGcc                                                                                      >  1:3810637/1‑93 (MQ=255)
  cATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGcc                                                                                      >  1:1673132/1‑93 (MQ=255)
  cATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGcc                                                                                      >  2:5995082/1‑93 (MQ=255)
   aTTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGccc                                                                                     <  1:3554269/93‑1 (MQ=255)
    ttGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGcccc                                                                                    <  2:6263059/93‑1 (MQ=255)
     tGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCt                                                                                   <  2:3121700/93‑1 (MQ=255)
     tGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCt                                                                                   <  2:5489189/93‑1 (MQ=255)
     tGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCt                                                                                   <  2:962093/93‑1 (MQ=255)
     tGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCt                                                                                   <  1:514520/93‑1 (MQ=255)
        ccGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGAc                                                                                >  2:3183031/1‑93 (MQ=255)
          ggTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGt                                                                              <  1:949436/93‑1 (MQ=255)
          ggTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGt                                                                              <  1:1284902/93‑1 (MQ=255)
           gtgtAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTg                                                                             >  1:5798701/1‑93 (MQ=255)
            tgtAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGc                                                                            <  2:2203552/93‑1 (MQ=255)
               aaaaGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAg                                                                         >  1:1598440/1‑93 (MQ=255)
                aaaGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGc                                                                        >  1:1003595/1‑93 (MQ=255)
                 aaGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCt                                                                       <  1:791602/93‑1 (MQ=255)
                 aaGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCt                                                                       <  1:5778061/93‑1 (MQ=255)
                 aaGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCt                                                                       >  1:5329143/1‑93 (MQ=255)
                 aaGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCt                                                                       <  1:5160314/93‑1 (MQ=255)
                  aGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTg                                                                      <  2:6345556/93‑1 (MQ=255)
                  aGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTg                                                                      >  1:4862195/1‑93 (MQ=255)
                   ggggTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGc                                                                     >  2:4428403/1‑93 (MQ=255)
                    gggTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCa                                                                    <  2:2451632/93‑1 (MQ=255)
                    gggTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCa                                                                    >  2:5919530/1‑93 (MQ=255)
                      gTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCAcg                                                                  <  2:5388362/93‑1 (MQ=255)
                       ttAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCAcgc                                                                 <  1:1930887/93‑1 (MQ=255)
                       ttAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCAcgc                                                                 <  2:3811329/93‑1 (MQ=255)
                            gagGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGt                                                            >  2:5618733/1‑93 (MQ=255)
                            gagGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGt                                                            <  2:3025137/93‑1 (MQ=255)
                            gagGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGt                                                            <  1:4967510/93‑1 (MQ=255)
                             agGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGtt                                                           >  2:4998629/1‑93 (MQ=255)
                             agGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGtt                                                           <  2:3906630/93‑1 (MQ=255)
                             agGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGtt                                                           <  1:3991749/93‑1 (MQ=255)
                              ggCGTCGTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTc                                                          <  1:2871691/93‑1 (MQ=255)
                               gCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCg                                                         <  2:335208/93‑1 (MQ=255)
                               gCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCg                                                         >  2:4781276/1‑93 (MQ=255)
                                cGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGa                                                        >  2:5233588/1‑93 (MQ=255)
                                 gTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGaa                                                       >  1:1506767/1‑93 (MQ=255)
                                  tCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAAc                                                      >  2:6038057/1‑93 (MQ=255)
                                  tCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAAc                                                      >  1:3967728/1‑93 (MQ=255)
                                  tCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAAc                                                      >  2:5989693/1‑93 (MQ=255)
                                  tCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAAc                                                      <  2:2497282/93‑1 (MQ=255)
                                    ttCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTc                                                    >  2:821456/1‑93 (MQ=255)
                                    ttCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTc                                                    >  1:5007054/1‑93 (MQ=255)
                                    ttCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTc                                                    <  2:5557939/93‑1 (MQ=255)
                                    ttCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTc                                                    <  2:518462/93‑1 (MQ=255)
                                     tCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCg                                                   >  1:5706834/1‑93 (MQ=255)
                                     tCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCg                                                   >  1:3368211/1‑93 (MQ=255)
                                     tCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCg                                                   <  1:1696711/93‑1 (MQ=255)
                                      cATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGt                                                  >  2:1277384/1‑93 (MQ=255)
                                       aTTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGtt                                                 >  2:5755897/1‑93 (MQ=255)
                                        ttGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTc                                                >  2:2959579/1‑93 (MQ=255)
                                        ttGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTc                                                <  1:1316878/93‑1 (MQ=255)
                                         tGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCa                                               <  2:2287885/93‑1 (MQ=255)
                                          gTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAg                                              >  1:5559053/1‑93 (MQ=255)
                                           tCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAgc                                             <  1:3192761/93‑1 (MQ=255)
                                            cAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAgcg                                            >  2:415001/1‑93 (MQ=255)
                                              gACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAGCGcc                                          >  2:1869845/1‑93 (MQ=255)
                                                cagcagAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAGCGCctc                                        <  2:3682115/93‑1 (MQ=255)
                                                 agcagAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAGCGGctct                                       >  2:3439120/1‑93 (MQ=255)
                                                 agcagAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAGCGCctct                                       <  1:4946123/93‑1 (MQ=255)
                                                  gcagAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCtt                                      <  2:3416784/93‑1 (MQ=255)
                                                  gcagAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCAGCGTTCGAACTCGTTCAGAGCCTCtt                                      >  2:5286753/1‑93 (MQ=255)
                                                   cagAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTc                                     <  1:4691883/93‑1 (MQ=255)
                                                   cagAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTc                                     <  2:5019861/93‑1 (MQ=255)
                                                    agAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTcc                                    >  2:4888446/1‑93 (MQ=255)
                                                     gAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCa                                   <  1:5491023/93‑1 (MQ=255)
                                                     gAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCa                                   >  1:4582407/1‑93 (MQ=255)
                                                     gAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCa                                   >  1:673759/1‑93 (MQ=255)
                                                       aTTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGc                                 >  1:53071/1‑93 (MQ=255)
                                                          tGTCCGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGCGGc                              <  1:1984316/93‑1 (MQ=255)
                                                          tGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGCGGc                              <  1:1889505/93‑1 (MQ=255)
                                                          tGTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGCGGc                              >  1:2655425/1‑93 (MQ=255)
                                                           gTACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGCGGCa                             >  2:1470439/1‑93 (MQ=255)
                                                            tACGCGCTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGCGGCAg                            <  2:606347/93‑1 (MQ=255)
                                                               gcgcTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGCGGCAGGTc                         <  2:4083583/93‑1 (MQ=255)
                                                               gcgcTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGCGGCAGGTc                         <  1:6091760/93‑1 (MQ=255)
                                                               gcgcTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGCGGCAGGTc                         >  2:2865064/1‑93 (MQ=255)
                                                                cgcTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGCGGCAGGTCg                        >  2:2403239/1‑93 (MQ=255)
                                                                cgcTGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGCGGCAGGTCg                        >  1:4126507/1‑93 (MQ=255)
                                                                   tGTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGCGGCAGGTCGCCa                     <  1:1418525/93‑1 (MQ=255)
                                                                    gTTCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGCGGCAGGTCGCCAc                    <  1:1193638/93‑1 (MQ=255)
                                                                     ttCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGCGGCAGGTCGCCACt                   <  2:1913712/93‑1 (MQ=255)
                                                                     ttCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGCGGCAGGTCGCCACt                   <  1:4654800/93‑1 (MQ=255)
                                                                      tCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGCGGCAGGTCGCCACtt                  <  1:336706/93‑1 (MQ=255)
                                                                      tCGGCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGCGGCAGGTCGCCACtt                  >  1:245724/1‑93 (MQ=255)
                                                                       cGGCTTTTTGTAATTTGGTCTGCCCCTGCCGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGCGGCAGGTCGCCACttt                 <  2:2789887/93‑1 (MQ=255)
                                                                        ggCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTc                >  1:2205686/1‑93 (MQ=255)
                                                                        ggCTTGTTGTAATTTGGTCTGCCCCTGACGTGCAAGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGCGGCAGGTCGCCCCTTTc                >  2:2011358/1‑93 (MQ=255)
                                                                         gCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTcc               <  2:854294/93‑1 (MQ=255)
                                                                         gCTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGCGGCAGGGCGGCACCTTcc               >  1:2370858/1‑93 (MQ=255)
                                                                          cTTGTTGTAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCa              >  2:112579/1‑93 (MQ=255)
                                                                              ttgtAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACg          >  2:1410614/1‑93 (MQ=255)
                                                                                 tAATTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACGGGt       <  1:4902045/93‑1 (MQ=255)
                                                                                  aaTTTGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACGGGtt      >  2:6427440/1‑93 (MQ=255)
                                                                                    tttGGTGTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACGGGTTAc    <  1:6046539/93‑1 (MQ=255)
                                                                                    tttGGTCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACGGGTTAc    <  2:720452/93‑1 (MQ=255)
                                                                                     ttGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACGGGTTACa   <  2:1803669/93‑1 (MQ=255)
                                                                                         |                                                                                         
CTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACGGGTTACAA  >  NC_000913/440328‑440506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑