BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,017,086	T→C	H215H (CAT→CAC)	ubiE →	UbiE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,017,086	0	T	C	99.6%	881.6 / ‑4.6	252	H215H (CAT→CAC)	ubiE	UbiE
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (136/114); total (136/116)
Fisher's exact test for biased strand distribution p-value = 4.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

Only 100 of 271 total aligned reads displayed.

ATACTCCTTCCATGTGCTGCCGCGTATTGGCTCACTGGTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCATCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCGGATTCGAAAGTGTCGACTACTACAATCTGACGGCAGGGGTTGTGGCGCT  >  NC_000913/4016996‑4017175

aTACTCCTTCCATGTGCTGCCGCGTATTGGCTCACTGGTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCAccc                                                                                         >  1:1247174/1‑93 (MQ=255)

aTACTCCTTCCATGTGCTGCCGCGTATTGGCTCACTGGTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCAccc                                                                                         <  2:725343/93‑1 (MQ=255)

aTACTCCTTCCATGTGCTGCCGCGTATTGGCTCACTGGTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCAccc                                                                                         <  1:1713313/93‑1 (MQ=255)

 tACTCCTTCCATGTGCTGCCGCGTATTGGCTCACTGGTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCAcccc                                                                                        >  1:1313667/1‑93 (MQ=255)

 tACTCCTTCCATGTGCTGCCGCGTATTGGCTCACCGGTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCAcccc                                                                                        >  2:2075929/1‑93 (MQ=255)

  aCTCCTTCCATGTGCTGCCGCGTATTGGCTCACTGGTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCg                                                                                       <  1:2513272/93‑1 (MQ=255)

     ccTTCCATGTGCTGCCGCGTATTGGCTCACTGGTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATc                                                                                    <  2:2026750/93‑1 (MQ=255)

      cTTCCATGTGCTGCCGCGTATTGGCTCACTGGTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCa                                                                                   >  2:5587221/1‑93 (MQ=255)

       ttCCATGTGCTGCCGCGTATTGGCTCACTGGTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAg                                                                                  <  1:3136805/93‑1 (MQ=255)

       ttCCATGTGCTGCCGCGTATTGGCTCACTGGTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAg                                                                                  <  2:855843/93‑1 (MQ=255)

        tCCATGTGCTGCCGCGTATTGGCTCACTGGTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAgg                                                                                 >  1:3743480/1‑93 (MQ=255)

         ccATGTGCTGCCGCGTATTGGCTCACTGGTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGa                                                                                <  2:2483056/93‑1 (MQ=255)

         ccATGTGCTGCCGCGTATTGGCTCACTGGTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGa                                                                                >  1:2328422/1‑93 (MQ=255)

          cATGTGCTGCCGCGTATTGGCTCACTGGTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGAt                                                                               >  2:5228046/1‑93 (MQ=255)

          cATGTGCTGCCGCGTATTGGCTCACTGGTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGAt                                                                               >  1:329964/1‑93 (MQ=255)

           aTGTGCTGCCGCGTATTGGCTCACTGGTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATa                                                                              >  2:4302252/1‑93 (MQ=255)

           aTGTGCTGCCGCGTATTGGCTCACTGGTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATa                                                                              >  1:6088355/1‑93 (MQ=255)

           aTGTGCTGCCGCGTATTGGCTCACTGGTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATa                                                                              >  1:4075711/1‑93 (MQ=255)

            tgtgCTGCCGCGTATTGGCTCACTGGTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATAc                                                                             >  1:921185/1‑93 (MQ=255)

            tgtgCTGCCGCGTATTGGCTCACTGGTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATAc                                                                             <  1:677538/93‑1 (MQ=255)

             gtgCTGCCGCGTATTGGCTCACTGGTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATAcc                                                                            <  1:5113420/93‑1 (MQ=255)

              tgctgcCGCGTATTGGCTCACTGGTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATAccc                                                                           <  1:3232014/93‑1 (MQ=255)

               gctgcCGCGTATTGGCTCACTGGTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATACCCt                                                                          >  1:5665488/1‑93 (MQ=255)

                 tgcCGCGTATTGGCTCACTGGTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATACCCTGa                                                                        <  2:4027981/93‑1 (MQ=255)

                  gcCGCGTATTGGCTCACTGGTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATACCCTGaa                                                                       >  2:4384008/1‑93 (MQ=255)

                  gcCGCGTATTGGCTCACTGGTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATACCCTGaa                                                                       >  1:1059633/1‑93 (MQ=255)

                   ccGCGTATTGGCTCACTGGTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATACCCTGaaa                                                                      <  2:598727/93‑1 (MQ=255)

                   ccGCGTATTGGCTCACTGGTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATACCCTGaaa                                                                      <  1:494531/93‑1 (MQ=255)

                    cgcgTATTGGCTCACTGGTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATACCCTGAAAg                                                                     <  2:6330833/93‑1 (MQ=255)

                     gcgTATTGGCTCACTGGTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATACCCTGAAAGc                                                                    >  2:5187211/1‑93 (MQ=255)

                      cgTATTGGCTCACTGGTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATACCCTGAAAGcc                                                                   >  2:4109179/1‑93 (MQ=255)

                       gTATTGGCTCACTGGTCGCGAACGACGCCGTCAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATACCCTGAAAGCCa                                                                  >  1:454407/1‑93 (MQ=255)

                        tATTGGCTCACTGGTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATACCCTGCAAGccct                                                                 >  1:158273/1‑91 (MQ=255)

                        tATTGGCTCACTGGTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATACCCTGAAAGCCat                                                                 >  1:1382352/1‑93 (MQ=255)

                        tATTGGCTCACTGGTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATACCCTGAAAGCCat                                                                 >  1:5594499/1‑93 (MQ=255)

                         aTTGGCTCACTGGTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATACCCTGAAAGCCatg                                                                <  1:154591/93‑1 (MQ=255)

                           tGGCTCACTGGTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATACCCTGAAAGCCatgat                                                              >  2:1725933/1‑93 (MQ=255)

                            ggCTCACTGGTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATACCCTGAAAGCCatgatg                                                             >  1:6383613/1‑93 (MQ=255)

                              cTCACTGGTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCa                                                           >  2:1820571/1‑93 (MQ=255)

                              cTCACTGGTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCa                                                           <  2:3706357/93‑1 (MQ=255)

                                 aCTGGTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGa                                                        <  2:4695064/93‑1 (MQ=255)

                                  cTGGTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGAt                                                       >  2:2901132/1‑93 (MQ=255)

                                  cTGGTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGAt                                                       <  2:3884873/93‑1 (MQ=255)

                                   tGGTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATg                                                      >  2:4370950/1‑93 (MQ=255)

                                    ggTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGc                                                     >  1:4039565/1‑93 (MQ=255)

                                    ggTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGc                                                     >  1:5945161/1‑93 (MQ=255)

                                     gTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGcc                                                    <  2:6205771/93‑1 (MQ=255)

                                      tCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCg                                                   <  2:2339938/93‑1 (MQ=255)

                                      gcgcgAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCg                                                   <  2:3282246/92‑1 (MQ=255)

                                       cgcgAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCgg                                                  <  1:6042582/93‑1 (MQ=255)

                                         cgAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATACCCTGAAAGCCGTGATGCAGGATGCCGGAt                                                <  1:5730617/93‑1 (MQ=255)

                                         cgAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCGGAt                                                >  1:2804996/1‑93 (MQ=255)

                                         cgAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCGGAt                                                <  2:3048918/93‑1 (MQ=255)

                                           aaCGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCGGATTc                                              >  1:2673200/1‑93 (MQ=255)

                                           aaCGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCGGATTc                                              <  1:3481597/93‑1 (MQ=255)

                                           aaCGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCGGATTc                                              >  2:2242969/1‑93 (MQ=255)

                                            acgacgCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCGGATTCg                                             <  1:473547/93‑1 (MQ=255)

                                               acgCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATATCCTGAAAGCCATGATGCAGGATGCCGGATTCGaaa                                          >  2:1514190/1‑93 (MQ=255)

                                               acgCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCGGATTCGaaa                                          >  2:3237465/1‑93 (MQ=255)

                                               acgCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCGGATTCGaaa                                          >  1:1967108/1‑93 (MQ=255)

                                               acgCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCGGATTCGaaa                                          >  1:5396626/1‑93 (MQ=255)

                                                   cGACATCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCGGATTCGAAAgtgt                                      <  1:2087670/93‑1 (MQ=255)

                                                     aCAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCGGATTCGAAAGTGTCg                                    <  2:5705852/93‑1 (MQ=255)

                                                     aCAGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCACGATACCCTGAAAGCCAAGATCCAGCAGGCCGGATTCGAAAGTGCCg                                    >  1:5385734/1‑93 (MQ=255)

                                                       aGCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCGGATTCGAAAGTGTCGac                                  <  1:1914193/93‑1 (MQ=255)

                                                        tcTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCGGATTCGAAAGTGTCGact                                 <  2:1442118/92‑1 (MQ=255)

                                                        gCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCGGATTCGAAAGTGTCGact                                 >  1:728398/1‑93 (MQ=255)

                                                        gCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCGGATTCGAAAGTGTCGact                                 >  2:3738673/1‑93 (MQ=255)

                                                        gCTACCGTTATCTGGCAGAATCCATCCGTATGCACCCCCATCAGGATACCCTGAAAGCCATGATGCAGGATGCCGGATTCGAAAGTGTCGact                                 <  2:2713239/93‑1 (MQ=255)

                                                          tACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCGGATTCGAAAGTGTCGactac                               <  2:4434543/93‑1 (MQ=255)

                                                          tACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCGGATTCGAAAGTGTCGactac                               >  2:3606077/1‑93 (MQ=255)

                                                          tACCGTTATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCGGATTCGAAAGTGTCGactac                               <  1:5777034/93‑1 (MQ=255)

                                                               ttATCTGGCAGAATCCATCCGTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCGGATTCGAAAGTGTCGACTACTACaa                          >  1:466745/1‑93 (MQ=255)

                                                                 aTCTGGCAGAATCCATCCGTATGCATCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCGGATTCGAAAGTGTCGACTACTACAATc                        <  2:639001/93‑1 (MQ=255)

                                                                 aTCTGGCAGAATCCATCCGTATGCAACCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCGGATTCGAAAGTGTCGACTACTACAATc                        <  2:6133873/93‑1 (MQ=255)

                                                                    tGGCAGAATCCATCCGTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCGGATTCGAAAGTGTCGACTACTACAATCTGa                     >  1:5970167/1‑93 (MQ=255)

                                                                     ggCAGAATCCATCCGTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCGGATTCGAAAGTGTCGACTACTACACTCTGAc                    >  1:3933648/1‑93 (MQ=255)

                                                                      gCAGAATCCATCCGTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCGGATTCGAAAGTGTCGACTACTACAATCTGACg                   <  2:4778859/93‑1 (MQ=255)

                                                                        aGAATCCATCCGTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCGGATTCGAAAGTGTCGACTACTACAATCTGACGGc                 >  2:129908/1‑93 (MQ=255)

                                                                        aGAATCCATCCGTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCGGATTCGAAAGTGTCGACTACTACAATCTGACGGc                 >  2:668808/1‑93 (MQ=255)

                                                                         gAATCCATCCGTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCGGATTCGAAAGTGTCGACTACTACAATCTGACGGCa                >  2:4174699/1‑93 (MQ=255)

                                                                         gAATCCATCCGTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCGGATTCGAAAGTGTCGACTACTACAATCTGACGGCa                >  1:1598608/1‑93 (MQ=255)

                                                                         gAATCCATCCGTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCGGATTCGAAAGTGTCGACTACTACAATCTGACGGCa                >  1:648302/1‑93 (MQ=255)

                                                                         gAATCCATCCGTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCGGATTCGAAAGTGTCGACTACTACAATCTGACGGCa                >  1:5553668/1‑93 (MQ=255)

                                                                         gAATCCATCCGTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCGGATTCGAAAGTGTCGACTACTACAATCTGACGGCa                <  2:5061370/93‑1 (MQ=255)

                                                                          aaTCCATCCGTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCGGATTCGAAAGTGTCGACTACTACAATCTGACGGCAg               <  1:5007674/93‑1 (MQ=255)

                                                                           atccatccGTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCGGATTCGAAAGTGTCGACTACTACAATCTGACGGCAgg              <  1:1159813/93‑1 (MQ=255)

                                                                           atccatccGTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCGGATTCGAAAGTGTCGACTACTACAATCTGACGGCAgg              <  2:1560221/93‑1 (MQ=255)

                                                                            tccatccGTATGCACCGCGATCAGGATAGCTTGAAAGCCATGATGCAGGATGCCGGATTCGAAAGTGTCGACTACTACAATCTGACGGCAgtg             <  1:3077610/93‑3 (MQ=255)

                                                                            tccatccGTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCGGATTCGAAAGTGTCGACTACTACAATCTGACGGCAggg             <  2:5259420/93‑1 (MQ=255)

                                                                            tccatccGTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCGGATTCGAAAGTGTCGACTACTACAATCTGACGGCAggg             >  1:4841690/1‑93 (MQ=255)

                                                                              catccGTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCGGATTCGAAAGTGTCGACTACTACAATCTGACGGCAGGGGt           <  2:1681422/93‑1 (MQ=255)

                                                                              catccGTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCGGATTCGAAAGTGTCGACTACTACAATCTGACGGCAGGGGt           <  2:6030993/93‑1 (MQ=255)

                                                                               atccGTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCGGATTCGAAAGTGTCGACTACTACAATCTGACGGCAGGGGtt          >  2:5807968/1‑93 (MQ=255)

                                                                               atccGTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCGGATTCGAAAGTGTCGACTACTACAATCTGACGGCAGGGGtt          <  1:2213998/93‑1 (MQ=255)

                                                                                tccGTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCGGATTCGAAAGTGTCGACTACTACAATCTGACGGCAGGGGTtg         <  1:2920328/93‑1 (MQ=255)

                                                                                   gTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCGGCTTCGAAAGTGTCGACTACTACAATCTCACGGCAGGGGTTGTgg      >  1:4140566/1‑93 (MQ=255)

                                                                                   gTATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCGGATTCGAAAGTGTCGACTACTACAATCTGACGGCAGGGGTTGTgg      <  2:3511641/93‑1 (MQ=255)

                                                                                    tATGCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCGGATTCGAAAGTGTCGACTACTACAATCTGACGGCAGGGGTTGTGgc     >  1:1786894/1‑93 (MQ=255)

                                                                                       gCACCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCGGATTCGAAAGTGTCGACTACTACAATCTGACGGCAGGGGTTGTGGCGCt  >  1:2962757/1‑93 (MQ=255)

ATACTCCTTCCATGTGCTGCCGCGTATTGGCTCACTGGTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCATCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCGGATTCGAAAGTGTCGACTACTACAATCTGACGGCAGGGGTTGTGGCGCT  >  NC_000913/4016996‑4017175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`