BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	484,107	A→G	L246P (CTG→CCG)	acrA ←	acridine efflux pump

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	484,107	0	A	G	99.7%	1007.0 / ‑5.0	286	L246P (CTG→CCG)	acrA	acridine efflux pump
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (135/150); total (136/150)
Fisher's exact test for biased strand distribution p-value = 4.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.05e-01

Only 100 of 289 total aligned reads displayed.

AGGGTGATAGACCCAGTGGTCTGATCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCAGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAGGAAGTCGTTGCTGGACTGGGTC  >  NC_000913/484017‑484196

aGGGTGATAGACCCAGTGGTCTGATCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGt                                                                                         <  2:6094577/93‑1 (MQ=255)

 gggTGATAGACCCAGTGGTCTGATCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTg                                                                                        <  1:4546070/93‑1 (MQ=255)

  ggTGATAGACCCAGTGGTCTGATCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGa                                                                                       >  1:3122379/1‑93 (MQ=255)

  ggTGATAGACCCAGTGGTCTGATCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGa                                                                                       >  1:2326400/1‑93 (MQ=255)

     gATAGACCCAGTGGTCTGATCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGacac                                                                                    <  2:1877472/93‑1 (MQ=255)

      aTAGACCCAGTGGTCTGATCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACt                                                                                   <  1:816728/93‑1 (MQ=255)

       tAGACCCAGTGGTCTGATCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACtt                                                                                  >  2:5843449/1‑93 (MQ=255)

         gACCCAGTGGTCTGATCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTg                                                                                >  2:5905860/1‑93 (MQ=255)

         gACCCAGTGGTCTGATCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTg                                                                                <  1:1140962/93‑1 (MQ=255)

         gACCCAGTGGTCTGATCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTg                                                                                >  2:4370570/1‑93 (MQ=255)

          aCCCAGTGGTCTGATCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTgg                                                                               <  1:548726/93‑1 (MQ=255)

           cccAGTGGTCTGATCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGc                                                                              <  1:3371755/93‑1 (MQ=255)

           cccAGTGGTCTGATCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGc                                                                              >  1:1714125/1‑93 (MQ=255)

             cAGTGGTCTGATCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCtt                                                                            <  1:2455188/93‑1 (MQ=255)

             cAGTGGTCTGATCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCtt                                                                            <  2:4613926/93‑1 (MQ=255)

             cAGTGGTCTGATCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCtt                                                                            >  1:5361738/1‑93 (MQ=255)

             cAGTGGTCTGATCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCtt                                                                            <  1:1281608/93‑1 (MQ=255)

             cAGTGGGCTGATCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCtt                                                                            <  1:2624929/93‑1 (MQ=255)

              aGTGGTCTGATCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCttt                                                                           <  2:6356884/93‑1 (MQ=255)

              aGTGGTCTGATCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCttt                                                                           >  1:3161403/1‑93 (MQ=255)

              aGTGGTCTGATCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCttt                                                                           <  1:4993042/93‑1 (MQ=255)

               gTGGTCTGATCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTg                                                                          >  2:949072/1‑93 (MQ=255)

               gTGGTCTGATCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTg                                                                          <  1:642079/93‑1 (MQ=255)

                tGGTCTGATCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGc                                                                         >  2:5568451/1‑93 (MQ=255)

                   tCTGATCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGt                                                                      <  2:2524691/93‑1 (MQ=255)

                   tCTGATCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGt                                                                      >  1:4322774/1‑93 (MQ=255)

                     tGATCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCTGTGACACTTTGGCTTTGCCGTtc                                                                    >  1:6336572/1‑93 (MQ=255)

                     tGATCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTtc                                                                    <  1:3983995/93‑1 (MQ=255)

                     tGATCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTtc                                                                    >  2:6386073/1‑93 (MQ=255)

                      gATCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTtct                                                                   <  1:6251197/93‑1 (MQ=255)

                       aTCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTtctc                                                                  <  1:672626/93‑1 (MQ=255)

                       aTCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTtctc                                                                  >  2:4751778/1‑93 (MQ=255)

                        tCAACGGTAACGTCAGAGAATTCCAGCGTCCCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTctct                                                                 <  1:4412406/93‑1 (MQ=255)

                        tCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTctct                                                                 <  2:6169245/93‑1 (MQ=255)

                         cAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCtt                                                                <  1:473626/93‑1 (MQ=255)

                         cAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCAGTGACACTTTGGCTTTGCCGTTCTCtt                                                                >  1:2110709/1‑93 (MQ=255)

                          aaCGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTACTGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTg                                                               >  1:5178557/1‑93 (MQ=255)

                          aaCGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTg                                                               >  1:5916588/1‑93 (MQ=255)

                           aCGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGt                                                              >  1:1813456/1‑93 (MQ=255)

                            cGGTACCGACAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGCCACTTTGGCTTTGCCGTTCTCTTGtt                                                             <  2:5435069/93‑1 (MQ=255)

                            cGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGtt                                                             >  2:3153895/1‑93 (MQ=255)

                              gTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTc                                                           >  2:5501351/1‑93 (MQ=255)

                              gTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTc                                                           >  1:2679967/1‑93 (MQ=255)

                                aaCGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAg                                                         <  2:3811190/93‑1 (MQ=255)

                                aaCGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAg                                                         >  1:2215048/1‑93 (MQ=255)

                                 aCGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGc                                                        >  2:3043350/1‑93 (MQ=255)

                                 aCGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGc                                                        >  1:4078738/1‑93 (MQ=255)

                                  cGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCg                                                       >  2:4066580/1‑93 (MQ=255)

                                   gTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGt                                                      <  2:870783/93‑1 (MQ=255)

                                      agagAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGcc                                                   <  2:3716893/93‑1 (MQ=255)

                                      agagAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGcc                                                   >  2:1698222/1‑93 (MQ=255)

                                        agaATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCAt                                                 >  1:1904524/1‑93 (MQ=255)

                                         gaATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCAtt                                                <  2:2149273/93‑1 (MQ=255)

                                          aaTTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTc                                               >  2:5028176/1‑93 (MQ=255)

                                          aaTTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTc                                               >  2:2572605/1‑93 (MQ=255)

                                           aTTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCg                                              <  1:4184349/93‑1 (MQ=255)

                                           aTTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCg                                              >  2:1007613/1‑93 (MQ=255)

                                             tCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGcc                                            <  2:3573126/93‑1 (MQ=255)

                                                aGCGTACCGTCCTGCGGGAACTTAATGCCGTGACTGTTGATCGGGGACACTTTGGCTTTTCCGTTCTCTTGTTTCAGCGTGCCATTCGCTAGt                                         >  2:6235935/1‑93 (MQ=255)

                                                 gCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGtt                                        <  2:261207/93‑1 (MQ=255)

                                                 gCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGtt                                        <  2:275332/93‑1 (MQ=255)

                                                  cGTACCGTCCTGCGGGAACTTAATGCCGTCACTGCTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTc                                       <  1:3238031/93‑1 (MQ=255)

                                                   gTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTcc                                      >  2:3431652/1‑93 (MQ=255)

                                                    tACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCt                                     <  2:2238642/93‑1 (MQ=255)

                                                      ccGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGt                                   >  1:401572/1‑93 (MQ=255)

                                                       cGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGtt                                  >  1:4697300/1‑93 (MQ=255)

                                                        gTCCTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGttt                                 >  1:1408422/1‑93 (MQ=255)

                                                         ttcTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTc                                <  2:5226750/91‑1 (MQ=255)

                                                          ccTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCa                               <  1:2828531/93‑1 (MQ=255)

                                                           cTGCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAg                              <  1:2898987/93‑1 (MQ=255)

                                                             gCGGGAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGgc                            >  1:916608/1‑93 (MQ=255)

                                                               gggAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGgcgc                          >  1:2400572/1‑93 (MQ=255)

                                                               gggAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGgcgc                          >  2:3538199/1‑93 (MQ=255)

                                                                ggAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCa                         >  2:1562673/1‑93 (MQ=255)

                                                                 gAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAg                        <  2:2841699/93‑1 (MQ=255)

                                                                 gAACTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAg                        >  1:377907/1‑93 (MQ=255)

                                                                  aaCTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAgg                       >  2:601431/1‑93 (MQ=255)

                                                                  aaCTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAgg                       >  1:5691665/1‑93 (MQ=255)

                                                                   aCTTAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAGGa                      >  1:6404267/1‑93 (MQ=255)

                                                                     ttAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAGGAAg                    <  1:3418081/93‑1 (MQ=255)

                                                                      tAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAGGAAGt                   <  1:256679/93‑1 (MQ=255)

                                                                      tAATGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAGGAAGt                   <  2:3262404/93‑1 (MQ=255)

                                                                       aaTGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAGGAAGTc                  <  1:5856053/93‑1 (MQ=255)

                                                                       aaTGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAGGAAGTc                  >  1:4833275/1‑93 (MQ=255)

                                                                        aTGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAGGAAGTCg                 >  2:5360274/1‑93 (MQ=255)

                                                                         tGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAGGAAGTCGt                <  1:4492073/93‑1 (MQ=255)

                                                                         tGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAGGAAGTCGt                <  1:1109283/93‑1 (MQ=255)

                                                                         tGCCGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAGGAAGTCGt                <  2:4100214/93‑1 (MQ=255)

                                                                            cGTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAGGAAGTCGTTGc             >  2:3960882/1‑93 (MQ=255)

                                                                             gTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAGGAAGTCGTTGCt            <  1:6131428/93‑1 (MQ=255)

                                                                             gTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAGGAAGTCGTTGCt            <  2:1224900/93‑1 (MQ=255)

                                                                             gTCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGATTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAGGAAGTCGTTGCt            >  2:4529196/1‑93 (MQ=255)

                                                                              tCACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAGGAAGTCGTTGCTg           <  2:669/93‑1 (MQ=255)

                                                                               cACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAGGAAGTCGTTGCTgg          >  2:526809/1‑93 (MQ=255)

                                                                               cACTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAGGAAGTCGTTGCTgg          >  2:4873245/1‑93 (MQ=255)

                                                                                 cTGTTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAGGAAGTCGTTGCTGGAc        <  2:5077046/93‑1 (MQ=255)

                                                                                 cTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAGGAAGTCGTTGCTGGAc        <  1:48011/93‑1 (MQ=255)

                                                                                 cTGGTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAGGAAGTCGTTGCTGGAc        <  1:1495687/93‑1 (MQ=255)

                                                                                    gTGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAGGAAGTCGTTGCTGGACTgg     <  2:4322774/93‑1 (MQ=255)

                                                                                     tGATCGGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAGGAAGTCGTTGCTGGACTggg    >  2:1344687/1‑93 (MQ=255)

AGGGTGATAGACCCAGTGGTCTGATCAACGGTAACGTCAGAGAATTCCAGCGTACCGTCCTGCGGGAACTTAATGCCGTCACTGGTGATCAGTGACACTTTGGCTTTGCCGTTCTCTTGTTTCAGCGTGCCATTCGCCAGTTCCTGTTTCAGGCGCAGGAAGTCGTTGCTGGACTGGGTC  >  NC_000913/484017‑484196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`