BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,244,613	A→G	Y84C (TAC→TGC)	malK →	ATP‑binding component of transport system for maltose

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,244,613	0	A	G	100.0%	876.2 / NA	249	Y84C (TAC→TGC)	malK	ATP‑binding component of transport system for maltose
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (135/114); total (135/114)

Only 100 of 258 total aligned reads displayed.

CGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTAACCAACGCG  >  NC_000913/4244523‑4244702

cGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGcg                                                                                         >  2:3609181/1‑93 (MQ=255)

  aTCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGcgcg                                                                                       <  2:6255181/93‑1 (MQ=255)

  aTCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGGATGGTGTTTCAGTCTTGcgcg                                                                                       >  2:1480264/1‑93 (MQ=255)

    cACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGct                                                                                     <  2:4795948/93‑1 (MQ=255)

     aCCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGctc                                                                                    >  2:1436308/1‑93 (MQ=255)

     aCCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGctc                                                                                    >  2:2608945/1‑93 (MQ=255)

     aCCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGctc                                                                                    >  1:3743023/1‑93 (MQ=255)

      ccAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGctct                                                                                   <  2:3173309/93‑1 (MQ=255)

       cAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTa                                                                                  >  1:2200108/1‑93 (MQ=255)

         gcggcgACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATc                                                                                <  1:1124544/93‑1 (MQ=255)

         gcggcgACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATc                                                                                >  1:5908815/1‑93 (MQ=255)

         gcggcgACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATc                                                                                >  2:2221172/1‑93 (MQ=255)

         gcggcgACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATc                                                                                <  1:3708591/93‑1 (MQ=255)

         gcggcgACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGGATGGTGTTTCAGTCTTGCGCGCtctctc                                                                                >  1:5822470/1‑93 (MQ=255)

          cggcgACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATcc                                                                               <  2:207781/93‑1 (MQ=255)

           ggcgACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGACCGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATccc                                                                              <  2:4517228/93‑1 (MQ=255)

           ggcgACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATccc                                                                              <  1:1590802/93‑1 (MQ=255)

            gcgACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATcccc                                                                             <  1:1465521/93‑1 (MQ=255)

            gcgACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATcccc                                                                             <  1:3856686/93‑1 (MQ=255)

               aCCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCAcc                                                                          <  2:1184168/93‑1 (MQ=255)

               aCCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCAcc                                                                          <  2:6172933/93‑1 (MQ=255)

                ccTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCt                                                                         >  1:643868/1‑93 (MQ=255)

                 cTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTg                                                                        >  1:3922292/1‑93 (MQ=255)

                  tGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGt                                                                       <  2:3474404/93‑1 (MQ=255)

                  tGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGt                                                                       >  2:2365688/1‑93 (MQ=255)

                   gTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTc                                                                      >  2:5832368/1‑93 (MQ=255)

                     tCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAg                                                                    >  2:2389662/1‑93 (MQ=255)

                     tCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAg                                                                    <  1:4496886/93‑1 (MQ=255)

                          ggTGAGACACGGATGAATGACCCTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCa                                                               <  2:2593635/93‑1 (MQ=255)

                          ggTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCa                                                               >  2:6354770/1‑93 (MQ=255)

                            tGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGa                                                             >  2:4906303/1‑93 (MQ=255)

                            tGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGa                                                             >  1:1912686/1‑93 (MQ=255)

                             gagaAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGaa                                                            <  2:5314172/93‑1 (MQ=255)

                                aaaCGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAAc                                                         <  1:5059675/93‑1 (MQ=255)

                                aaaCGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAAc                                                         <  2:5655802/93‑1 (MQ=255)

                                 aaCGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACa                                                        <  2:1736004/93‑1 (MQ=255)

                                 aaCGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACa                                                        >  1:2656716/1‑93 (MQ=255)

                                  aCGGTTGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAGAACAt                                                       >  1:4769191/1‑93 (MQ=255)

                                  aCGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACAt                                                       >  2:3927537/1‑93 (MQ=255)

                                  aCGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACAt                                                       <  1:2706517/93‑1 (MQ=255)

                                  aCGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACAt                                                       <  1:2021610/93‑1 (MQ=255)

                                   cGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATg                                                      >  2:3319829/1‑93 (MQ=255)

                                   cGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATg                                                      <  1:720632/93‑1 (MQ=255)

                                    ggATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGt                                                     >  2:4700134/1‑93 (MQ=255)

                                     gATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTc                                                    >  2:4334295/1‑93 (MQ=255)

                                     gATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTc                                                    >  2:85025/1‑93 (MQ=255)

                                     gATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTc                                                    >  1:3215318/1‑93 (MQ=255)

                                      atgaatgaCACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCa                                                   >  1:5708763/1‑93 (MQ=255)

                                       tgaatgaCACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCAt                                                  >  1:4982536/1‑93 (MQ=255)

                                          atgaCACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTg                                               >  1:4060898/1‑93 (MQ=255)

                                           tgaCACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTgg                                              <  1:6460960/93‑1 (MQ=255)

                                            gaCACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGc                                             <  1:48426/93‑1 (MQ=255)

                                             acacTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGcc                                            <  1:1531172/93‑1 (MQ=255)

                                             acacTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGcc                                            >  2:3824071/1‑93 (MQ=255)

                                             acacTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGcc                                            >  2:2760348/1‑93 (MQ=255)

                                               acTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTg                                          >  1:2365488/1‑93 (MQ=255)

                                                cTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGa                                         <  1:1977532/93‑1 (MQ=255)

                                                 tCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGaa                                        >  2:6096017/1‑93 (MQ=255)

                                                  ccGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGaaa                                       >  1:1413489/1‑93 (MQ=255)

                                                   cGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAAc                                      >  1:2785397/1‑93 (MQ=255)

                                                    gCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCTCCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAAct                                     <  1:1830491/93‑1 (MQ=255)

                                                    gCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAAct                                     <  1:2908695/93‑1 (MQ=255)

                                                     ccAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAActg                                    >  2:1298579/1‑93 (MQ=255)

                                                     ccAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAActg                                    >  2:4928285/1‑93 (MQ=255)

                                                       agcagAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCGCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAActcgc                                  >  1:3107863/1‑93 (MQ=255)

                                                       agcagAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAActggc                                  <  2:5210725/93‑1 (MQ=255)

                                                       agcagAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAActggc                                  <  2:1385893/93‑1 (MQ=255)

                                                        gcagAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAActggct                                 <  1:5996686/93‑1 (MQ=255)

                                                        gcagAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAActggct                                 >  2:2881707/1‑93 (MQ=255)

                                                        gcagAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAActggct                                 <  2:1946761/93‑1 (MQ=255)

                                                          agAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAActggctgg                               <  1:4278225/93‑1 (MQ=255)

                                                           gAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACtggctggc                              <  2:2491714/93‑1 (MQ=255)

                                                           gAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACtggctggc                              >  1:2255136/1‑93 (MQ=255)

                                                           gAACGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACtggctggc                              >  1:5534967/1‑93 (MQ=255)

                                                             aCGCGGCGTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGgcgc                            >  1:3552745/1‑93 (MQ=255)

                                                                 ggcgTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCaaaa                        >  2:4103429/1‑93 (MQ=255)

                                                                    gTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTTGCGCcaaaaaa                     >  2:5452875/1‑93 (MQ=255)

                                                                    gTTGGTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCaaaaaaa                     >  2:6035855/1‑93 (MQ=255)

                                                                       ggTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAagag                  <  2:5132040/93‑1 (MQ=255)

                                                                       ggTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAagag                  >  2:1910637/1‑93 (MQ=255)

                                                                       ggTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTAAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAagag                  >  1:990136/1‑93 (MQ=255)

                                                                        gTATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAgg                 <  1:267153/93‑1 (MQ=255)

                                                                         tATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAagacgg                >  1:6085459/1‑90 (MQ=255)

                                                                         tATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGt                <  1:5393741/93‑1 (MQ=255)

                                                                         tATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGt                >  1:4707619/1‑93 (MQ=255)

                                                                         tATGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGt                <  1:1742123/93‑1 (MQ=255)

                                                                         tATGGTGTTTCAGCCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGt                <  2:3665178/93‑1 (MQ=255)

                                                                          aTGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTg               >  2:3014571/1‑93 (MQ=255)

                                                                           tGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGa              >  2:3093134/1‑93 (MQ=255)

                                                                           tGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGa              <  2:2271329/93‑1 (MQ=255)

                                                                           tGGTGTTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGGGa              >  1:4424962/1‑93 (MQ=255)

                                                                             gtgtTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGAtt            <  2:5460374/93‑1 (MQ=255)

                                                                              tgtTTCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTa           >  1:884646/1‑93 (MQ=255)

                                                                                tttCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTAAc         >  1:525756/1‑93 (MQ=255)

                                                                                  tCAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTAACCa       >  2:120900/1‑93 (MQ=255)

                                                                                   cAGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTAACCaa      >  1:2482441/1‑93 (MQ=255)

                                                                                    aGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTAACCAAc     <  2:2017691/93‑1 (MQ=255)

                                                                                    aGTCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTAACCAAc     <  2:3447674/93‑1 (MQ=255)

                                                                                      tCTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTAACCAAcgc   <  1:3500093/93‑1 (MQ=255)

                                                                                       cTTGCGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTAACCAAcgcg  <  1:4919860/93‑1 (MQ=255)

CGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAGTAGCAGAAAACATGTCATTTGGCCTGAAACTGGCTGGCGCAAAAAAAGAGGTGATTAACCAACGCG  >  NC_000913/4244523‑4244702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`