Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,315,095 C→T A127T (GCT→ACT)  phnL ← ATP‑binding component of phosphonate transport

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,315,0950CT99.6% 991.8 / ‑5.5 259A127T (GCT→ACT) phnLATP‑binding component of phosphonate transport
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (122/136);  total (122/137)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01

Only 100 of 276 total aligned reads displayed.

CTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGATGACGCGCAGAAACT  >  NC_000913/4315006‑4315184
                                                                                         |                                                                                         
ctgctgTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAgtggc                                                                                        <  2:3487904/93‑1 (MQ=255)
ctgctgTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAgtggc                                                                                        >  2:2824435/1‑93 (MQ=255)
ctgctgTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAgtggc                                                                                        <  1:2176920/93‑1 (MQ=255)
 tgctgTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAgtggcg                                                                                       >  2:4719525/1‑93 (MQ=255)
  gctgTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGgcgc                                                                                      >  1:6430269/1‑93 (MQ=255)
   ctgTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCa                                                                                     <  1:5916117/93‑1 (MQ=255)
   ctgTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCa                                                                                     <  2:1258789/93‑1 (MQ=255)
    tgTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAg                                                                                    >  1:427903/1‑93 (MQ=255)
     gTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAgg                                                                                   <  1:3556857/93‑1 (MQ=255)
     gTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAgg                                                                                   >  1:2461297/1‑93 (MQ=255)
        cGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCCGGCtt                                                                                <  1:3613077/93‑1 (MQ=255)
        cGCCACCGGAAAATGTCGATGGTGACAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCtt                                                                                <  1:4586888/93‑1 (MQ=255)
         gCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTc                                                                               <  1:5341732/93‑1 (MQ=255)
         gCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTc                                                                               >  2:2851281/1‑93 (MQ=255)
          ccACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCa                                                                              <  2:1735240/93‑1 (MQ=255)
          ccACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCa                                                                              <  2:3207643/93‑1 (MQ=255)
           cACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCAc                                                                             >  1:6329629/1‑93 (MQ=255)
           cACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCAc                                                                             >  1:1046424/1‑93 (MQ=255)
            aCCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCAcg                                                                            <  2:1585269/93‑1 (MQ=255)
            aCCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCAcg                                                                            >  1:4789467/1‑93 (MQ=255)
            aCCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCAcg                                                                            <  1:4092217/93‑1 (MQ=255)
             ccGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCAcgc                                                                           <  2:671193/93‑1 (MQ=255)
             ccGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCAcgc                                                                           >  1:2720975/1‑93 (MQ=255)
                gAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGa                                                                        <  1:1151512/93‑1 (MQ=255)
                gAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGa                                                                        <  1:2869167/93‑1 (MQ=255)
                gAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGa                                                                        >  1:4988347/1‑93 (MQ=255)
                 aaaaTGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGaa                                                                       <  2:5833212/93‑1 (MQ=255)
                 aaaaTGCCGATGGTGCCAGGTGCCACAGGCGTTCCGGCCCGCTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGaa                                                                       >  2:2752781/1‑93 (MQ=255)
                  aaaTGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAAc                                                                      <  1:2600325/93‑1 (MQ=255)
                  aaaTGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAAc                                                                      >  1:6142146/1‑93 (MQ=255)
                   aaTGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACg                                                                     >  2:3247359/1‑93 (MQ=255)
                   aaTGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCACGCCGAACg                                                                     >  1:3491613/1‑93 (MQ=255)
                    aTGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACGc                                                                    <  2:3067558/93‑1 (MQ=255)
                     tGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACGcc                                                                   <  1:55691/93‑1 (MQ=255)
                     tGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACGcc                                                                   <  1:22255/93‑1 (MQ=255)
                       tCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACGCCgg                                                                 >  2:6250772/1‑93 (MQ=255)
                         gATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTa                                                               >  2:5204922/1‑93 (MQ=255)
                          aTGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTAt                                                              <  2:4833043/93‑1 (MQ=255)
                           tGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATc                                                             <  1:5307421/93‑1 (MQ=255)
                             gtgccaggtgccaCAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGa                                                           <  2:6299085/93‑1 (MQ=255)
                                ccaggtgccaCAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGagca                                                        >  2:1041736/1‑93 (MQ=255)
                                 caggtgccaCAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGagcag                                                       <  1:1380250/93‑1 (MQ=255)
                                  aggtgccaCAGGCGTGCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGagcagc                                                      <  1:5680954/93‑1 (MQ=255)
                                   ggtgccaCAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCg                                                     >  2:5117042/1‑93 (MQ=255)
                                   ggtgccaCAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCg                                                     >  1:200109/1‑93 (MQ=255)
                                     tgccaCAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGc                                                   <  2:6446964/93‑1 (MQ=255)
                                      gccaCAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCt                                                  >  2:3972224/1‑93 (MQ=255)
                                       ccaCAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTg                                                 >  1:4496919/1‑93 (MQ=255)
                                          caGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCAt                                              <  2:4216611/93‑1 (MQ=255)
                                           cggCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATc                                             <  1:3942019/92‑1 (MQ=255)
                                           aGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATc                                             <  1:1933402/93‑1 (MQ=255)
                                            ggCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATca                                            <  1:3072974/93‑1 (MQ=255)
                                             gCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATcac                                           <  2:5306453/93‑1 (MQ=255)
                                               gTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATcacca                                         <  1:896878/93‑1 (MQ=255)
                                               gTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATcacca                                         >  2:601793/1‑93 (MQ=255)
                                                ttCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATcaccac                                        >  2:6101791/1‑93 (MQ=255)
                                                 tCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACt                                       <  1:4199324/93‑1 (MQ=255)
                                                 tCCGGCACGTTCAGGCGGGTCAGAAGACGCGCGGGTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACt                                       <  1:4925442/93‑1 (MQ=255)
                                                   cGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTc                                     <  2:1522301/93‑1 (MQ=255)
                                                   cGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCCTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCCAACAGCGGCTGCATCACCACGTc                                     >  2:3270582/1‑93 (MQ=255)
                                                    ggCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTcc                                    >  1:3711716/1‑93 (MQ=255)
                                                     gCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCa                                   >  1:1252441/1‑93 (MQ=255)
                                                     gCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCa                                   >  1:1678610/1‑93 (MQ=255)
                                                      cACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAg                                  <  2:105336/93‑1 (MQ=255)
                                                       aCGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTTACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGt                                 >  1:5213454/1‑93 (MQ=255)
                                                       aCGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGt                                 >  2:1907526/1‑93 (MQ=255)
                                                       aCGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGt                                 <  2:3897658/93‑1 (MQ=255)
                                                            cAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTga                            >  2:4335263/1‑93 (MQ=255)
                                                            cAGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACCTCCAGTGCTga                            >  2:5776488/1‑93 (MQ=255)
                                                             aGGCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTgag                           >  2:3806972/1‑93 (MQ=255)
                                                               gCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGAt                         <  1:4729214/93‑1 (MQ=255)
                                                               gCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGAt                         <  1:1008905/93‑1 (MQ=255)
                                                               gCGGGTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGAt                         <  2:496335/93‑1 (MQ=255)
                                                                 gggTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATAc                       <  1:3249241/93‑1 (MQ=255)
                                                                 gggTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATAc                       <  1:2327018/93‑1 (MQ=255)
                                                                  ggTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATAcg                      <  2:2569645/93‑1 (MQ=255)
                                                                   gTGAGAAGACGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATAcgc                     <  1:5100449/93‑1 (MQ=255)
                                                                     gagaAGACGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCgg                   >  2:3615738/1‑93 (MQ=255)
                                                                      agaagaCGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCggg                  <  1:4615815/93‑1 (MQ=255)
                                                                      agaagaCGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCggg                  <  1:390227/93‑1 (MQ=255)
                                                                       gaagaCGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGa                 <  2:4067520/93‑1 (MQ=255)
                                                                        aagaCGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGAt                >  2:5570399/1‑93 (MQ=255)
                                                                         agaCGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGGTg               >  1:157362/1‑93 (MQ=255)
                                                                         agaCGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGATg               <  2:4436269/93‑1 (MQ=255)
                                                                          gaCGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGATGa              <  2:1342379/93‑1 (MQ=255)
                                                                          gaCGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGATGa              >  1:4684918/1‑93 (MQ=255)
                                                                           aCGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGATGAc             <  1:3590851/93‑1 (MQ=255)
                                                                           aCGCGCGGCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGATGAc             <  1:2973201/93‑1 (MQ=255)
                                                                            cgcgcgGCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGATGAcg            >  1:3853982/1‑93 (MQ=255)
                                                                             gcgcgGCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGATGAcgc           >  2:371266/1‑93 (MQ=255)
                                                                               gcgGCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGATGACgcgc         >  2:1989639/1‑93 (MQ=255)
                                                                                 ggCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGATGACGCGCAg       >  2:4522840/1‑93 (MQ=255)
                                                                                 ggCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGATGACGCGCAg       >  2:869840/1‑93 (MQ=255)
                                                                                  gCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGATGACGCGCAGa      >  1:5638321/1‑93 (MQ=255)
                                                                                  gCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGATGACGCGCAGa      >  1:5531990/1‑93 (MQ=255)
                                                                                  gCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGATGACGCGCAGa      >  1:975421/1‑93 (MQ=255)
                                                                                  gCTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGATGACGCGCAGa      <  2:3795306/93‑1 (MQ=255)
                                                                                   cTTTAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGATGACGCGCAGaa     >  2:2232529/1‑93 (MQ=255)
                                                                                    tttAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGATGACGCGCAGaaa    >  1:1851627/1‑93 (MQ=255)
                                                                                      tAGTGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGATGACGCGCAGAAACt  >  1:5445451/1‑93 (MQ=255)
                                                                                         |                                                                                         
CTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGCCGGTATCGAGCAGCGGCTGCATCACCACTTCCAGTGCTGAGATACGCGGGATGACGCGCAGAAACT  >  NC_000913/4315006‑4315184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑