BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	38,389	C→T	D243N (GAC→AAC)	caiB ←	l‑carnitine dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	38,389	0	C	T	98.7%	886.8 / ‑1.7	235	D243N (GAC→AAC)	caiB	l‑carnitine dehydratase
Reads supporting (aligned to +/- strand): ref base C (0/3); new base T (136/96); total (136/99)
Fisher's exact test for biased strand distribution p-value = 7.35e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.69e-01

Only 100 of 239 total aligned reads displayed.

CTGGCGTGCCAAGCAGATGTGCGAGGCCAATATCTTTAAAGCACTCTTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTCGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGTTGAAGTAATCCA  >  NC_000913/38299‑38478

cTGGCGTGCCAAGCAGATGTGCGAGGCCAATATCTTTAAAGCACTCTTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTgg                                                                                         >  1:5467890/1‑93 (MQ=255)

 tGGCGTGCCAAGCAGATGTGCGAGGCCAATATCTTTAAAGCACTCTTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGc                                                                                        >  1:4424522/1‑93 (MQ=255)

   gCGTGCCAAGCAGATGTGCGAGGCCAATATCTTTAAAGCACTCTTCAATTTGGGTAATGACCACCAGTTCCATCACGATGTAGCCGTTGGcac                                                                                      >  1:2991782/1‑93 (MQ=255)

      tGCCAAGCAGATGTGCGAGGCCAATATCTTTAAAGCACTCTTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACAtt                                                                                   <  2:4081343/93‑1 (MQ=255)

      tGCCAAGCAGATGTGCGAGGCCAATATCTTTAAAGCACTCTTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACAtt                                                                                   <  2:3122262/93‑1 (MQ=255)

        ccAAGCAGATGTGCGAGGCCAATATCTTTAAAGCACTCTTCAATTTGGGTAATGCCCTCCAGTTCCATCACGATATAGGCGTTGGCACATTta                                                                                 >  1:5093875/1‑93 (MQ=255)

        ccAAGCAGATGTGCGAGGCCAATATCTTTAAAGCACTCTTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTta                                                                                 <  2:4827545/93‑1 (MQ=255)

         cAAGCAGATGTGCGAGGCCAATATCTTTAAAGCACTCTTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTCGGCACATTtat                                                                                <  1:6009734/93‑1 (MQ=255)

          aaGCAGATGTGCGAGGCCAATATCTTTAAAGCACTCTTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTtata                                                                               >  2:5221675/1‑93 (MQ=255)

          aaGCAGATGTGCGAGGCCAATATCTTTAAAGCACTCTTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTtata                                                                               >  2:4426473/1‑93 (MQ=255)

          aaGCAGATGTGCGAGGCCAATATCTTTAAAGCACTCTTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTtata                                                                               >  2:4285110/1‑93 (MQ=255)

           aGCAGATGTGCGAGGCCAATATCTTTAAAGCACTCTTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATAc                                                                              >  1:3991287/1‑93 (MQ=255)

           aGCAGATGTGCGAGGCCAATATCTTTAAAGCACTCTTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATAc                                                                              >  2:5344604/1‑93 (MQ=255)

           aGCAGATGTGCGAGGCCAATATCTTTAAAGCACTCTTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATAc                                                                              >  1:72816/1‑93 (MQ=255)

             cAGATGTGCGAGGCCAATATCTTTAAAGCACTCTTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAg                                                                            >  2:2393023/1‑93 (MQ=255)

              aGATGTGCGAGGCCAATATCTTTAAAGCACTCTTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGa                                                                           >  1:5837759/1‑93 (MQ=255)

               gATGTGCGAGGCCAATATCTTTAAAGCACTCTTCAATTTGGGTAATGCCCTCCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGAc                                                                          <  2:3430057/93‑1 (MQ=255)

               gATGTGCGAGGCCAATATCTTTAAAGCACTCTTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGAc                                                                          <  2:3738479/93‑1 (MQ=255)

                aTGTGCGAGGCCAATATCTTTAAAGCACTCTTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGAcc                                                                         >  1:3315333/1‑93 (MQ=255)

                aTGTGCGAGGCAAATATCTTTAAAGCACTCTTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGAcc                                                                         <  1:1724652/93‑1 (MQ=255)

                   tgCGAGGCCAATATCTTTAAAGCACTCTTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCa                                                                      >  1:3952089/1‑93 (MQ=255)

                      gAGGCCAATATCTTTAAAGCACTCTTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAAcc                                                                   >  1:5814980/1‑93 (MQ=255)

                       aGGCCAATATCTTTAAAGCACTCTTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCg                                                                  <  2:331607/93‑1 (MQ=255)

                        ggCCAATATCTTTAAAGCACTCTTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCgg                                                                 >  1:1292619/1‑93 (MQ=255)

                           cAATATCTTTAAAGCACTCTTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCgt                                                              >  2:2950437/1‑93 (MQ=255)

                            aaTATCTTTAAAGCACTCTTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCgta                                                             >  2:4064396/1‑93 (MQ=255)

                            aaTATCTTTAAAGCACTCTTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCgta                                                             >  1:5384906/1‑93 (MQ=255)

                            aaTATCTTTAAAGCACTCTTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCgta                                                             >  1:2308459/1‑93 (MQ=255)

                            aaTATCTTTAAAGCACTCTTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCgta                                                             >  1:1000695/1‑93 (MQ=255)

                             atatCTTTAAAGCACTCTTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCgtag                                                            >  2:2009081/1‑93 (MQ=255)

                             atatCTTTAAAGCACTCTTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCgtag                                                            >  2:731432/1‑93 (MQ=255)

                             atatCTTTAAAGCACTCTTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGAACGCAACCGGCgtag                                                            >  1:249962/1‑93 (MQ=255)

                              tatCTTTAAAGCACTCTTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCgtagt                                                           >  1:1137478/1‑93 (MQ=255)

                              tatCTTTAAAGCACTCTTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCgtagt                                                           <  1:2136870/93‑1 (MQ=255)

                                tCTTTAAAGCACTCTTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGtagtag                                                         >  1:3053905/1‑93 (MQ=255)

                                tCTTTAAAGCACTCTTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGtagtag                                                         <  2:5047149/93‑1 (MQ=255)

                                 cTTTAAAGCACTCTTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAgg                                                        <  1:2640199/93‑1 (MQ=255)

                                 cTTTAAAGCACTCTTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAgg                                                        <  2:61694/93‑1 (MQ=255)

                                  tttAAAGCACTCTTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAggg                                                       >  2:4644175/1‑93 (MQ=255)

                                  tttAAAGCACTCTTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAggg                                                       <  1:2698463/93‑1 (MQ=255)

                                  tttAAAGCACTCTTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAggg                                                       >  2:1663671/1‑93 (MQ=255)

                                  tttAAAGCACTCTTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAggg                                                       >  1:5519614/1‑93 (MQ=255)

                                     aaaGCACTCTTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATc                                                    <  1:4153956/93‑1 (MQ=255)

                                     aaaGCACTCTTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATc                                                    <  1:2540114/93‑1 (MQ=255)

                                       aGCACTCTTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCtt                                                  <  1:4302664/93‑1 (MQ=255)

                                       aGCACTCTTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCtt                                                  >  2:4986458/1‑93 (MQ=255)

                                        gCACTCTTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCttt                                                 >  1:1382703/1‑93 (MQ=255)

                                         cACTCTTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTACGGATCTTTa                                                >  2:272157/1‑93 (MQ=255)

                                          cctctTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTAc                                               <  1:5627547/92‑1 (MQ=255)

                                            tctTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGCCCGCAACCGGCGTAGTAGGGATCTTTACCt                                             <  1:2743604/93‑1 (MQ=255)

                                            tctTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTCGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCt                                             <  1:616410/93‑1 (MQ=255)

                                             ctTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCtt                                            <  1:1895936/93‑1 (MQ=255)

                                             ctTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCtt                                            <  1:1802349/93‑1 (MQ=255)

                                              ttCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCttt                                           >  1:3743901/1‑93 (MQ=255)

                                              ttCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCttt                                           >  2:3842817/1‑93 (MQ=255)

                                              ttCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCttt                                           <  1:404657/93‑1 (MQ=255)

                                              ttCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCttt                                           <  1:3073569/93‑1 (MQ=255)

                                                cAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGc                                         <  1:6357448/93‑1 (MQ=255)

                                                 aaTTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCt                                        <  1:5250181/93‑1 (MQ=255)

                                                  aTTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTc                                       >  1:3470187/1‑93 (MQ=255)

                                                    ttGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCAt                                     >  2:2124119/1‑93 (MQ=255)

                                                     tGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATg                                    >  2:4324868/1‑93 (MQ=255)

                                                     tGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATg                                    <  1:4812069/93‑1 (MQ=255)

                                                     tGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATg                                    >  2:1445932/1‑93 (MQ=255)

                                                     tGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATg                                    <  2:5629394/93‑1 (MQ=255)

                                                     tGGGTAATGCCCACCAGTTACATCACGATGTAGCCGTCGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATg                                    <  2:1255383/93‑1 (MQ=255)

                                                      gggTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATgc                                   <  1:3788990/93‑1 (MQ=255)

                                                      gggTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATgc                                   >  2:6101168/1‑93 (MQ=255)

                                                      gggTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATgc                                   >  2:647909/1‑93 (MQ=255)

                                                       ggTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATgcg                                  >  2:1327665/1‑93 (MQ=255)

                                                        gTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATgcgc                                 >  1:4724553/1‑93 (MQ=255)

                                                        gTAATGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATgcgc                                 >  2:5275970/1‑93 (MQ=255)

                                                          aaTGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCgg                               >  2:107123/1‑93 (MQ=255)

                                                          aaTGCCCACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCgg                               >  1:3257545/1‑93 (MQ=255)

                                                              cccACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGcac                           <  1:276586/93‑1 (MQ=255)

                                                              cccACCAGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGcac                           <  2:4939335/93‑1 (MQ=255)

                                                                caccaGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACAt                         <  1:5350679/93‑1 (MQ=255)

                                                                 accaGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACAtt                        >  1:1342113/1‑93 (MQ=255)

                                                                  ccaGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACAttt                       <  2:153921/93‑1 (MQ=255)

                                                                   caGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTc                      <  2:3551452/93‑1 (MQ=255)

                                                                   caGTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTc                      <  1:3759227/93‑1 (MQ=255)

                                                                     gTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTcgc                    >  1:4832427/1‑93 (MQ=255)

                                                                     gTTCCATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTcgc                    >  1:2079848/1‑93 (MQ=255)

                                                                        ccATCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTcgccgc                 >  2:1114021/1‑93 (MQ=255)

                                                                          ctCACGATGTAGCCGTTGGCACATTTATACAGTCCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTCGcctccg               <  1:1245844/92‑1 (MQ=255)

                                                                          aTCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTCGccgccg               >  2:4556742/1‑93 (MQ=255)

                                                                           tCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGt              >  2:279763/1‑93 (MQ=255)

                                                                           tCACGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGt              <  2:1602702/93‑1 (MQ=255)

                                                                              cGATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGTTGa           >  1:3356226/1‑93 (MQ=255)

                                                                               gATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGTTGaa          <  2:3062275/93‑1 (MQ=255)

                                                                               gATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGTTGaa          >  1:607884/1‑93 (MQ=255)

                                                                               gATGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGTTGaa          >  1:604932/1‑93 (MQ=255)

                                                                                 tGTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGTTGAAGt        <  2:5562211/93‑1 (MQ=255)

                                                                                  gTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGTTGAAGTa       >  2:3653746/1‑93 (MQ=255)

                                                                                  gTAGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGTTGAAGTa       <  1:4340066/93‑1 (MQ=255)

                                                                                    aGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGTTGAAGTAAt     <  2:3470187/93‑1 (MQ=255)

                                                                                    aGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGTTGAAGTAAt     >  2:6062046/1‑93 (MQ=255)

                                                                                    aGCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGTTGAAGTAAt     >  1:4998236/1‑93 (MQ=255)

                                                                                     gCCGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGTTGAAGTAATc    >  1:3602480/1‑93 (MQ=255)

                                                                                       cGTTGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGTTGAAGTAATCca  <  2:1738245/93‑1 (MQ=255)

CTGGCGTGCCAAGCAGATGTGCGAGGCCAATATCTTTAAAGCACTCTTCAATTTGGGTAATGCCCACCAGTTCCATCACGATGTAGCCGTCGGCACATTTATACAGACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGTTGAAGTAATCCA  >  NC_000913/38299‑38478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`