BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,444,741	A→G	E1018G (GAG→GGG)	ytfN →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,444,741	0	A	G	99.7%	984.2 / NA	286	E1018G (GAG→GGG)	ytfN	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (139/146); minor base C (0/1); total (139/147)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 289 total aligned reads displayed.

GGGCGCGCATCGTGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGAGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCTGAAAGCGC  >  NC_000913/4444651‑4444830

gggCGCGCATCGTGGTGCACGATCTGCCGGAAAGCGCAGTAGGCTTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGc                                                                                         <  2:1625974/93‑1 (MQ=255)

gggCGCGCATCGTGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGc                                                                                         >  1:4825430/1‑93 (MQ=255)

    gcgcATCGTGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGaa                                                                                     >  1:5772799/1‑93 (MQ=255)

      gcATCGTGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACTATAACCTGCAACCGGAAGGGCCGaaaa                                                                                   <  1:3373804/93‑1 (MQ=255)

       cATCGTGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGaaaag                                                                                  >  2:1224353/1‑92 (MQ=255)

       cATCGTGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAAc                                                                                  <  2:4583124/93‑1 (MQ=255)

       cATCGTGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAAc                                                                                  <  2:5741540/93‑1 (MQ=255)

        aTCGTGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACg                                                                                 <  2:6344964/93‑1 (MQ=255)

         tCGTGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATATCCTGCAACCGGAAGGGCCCAAACCgg                                                                                <  1:2711380/93‑1 (MQ=255)

         tCGTGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACgg                                                                                <  2:398610/93‑1 (MQ=255)

          cGTGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGc                                                                               >  2:918125/1‑93 (MQ=255)

           gtggtgCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCg                                                                              >  2:3148476/1‑93 (MQ=255)

            tggtgCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGt                                                                             <  1:1995398/93‑1 (MQ=255)

             ggtgCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTc                                                                            >  2:1919405/1‑93 (MQ=255)

                 cACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGAtt                                                                        <  1:5444280/93‑1 (MQ=255)

                  aCGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTc                                                                       <  2:3226212/93‑1 (MQ=255)

                  aCGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTc                                                                       <  2:6421780/93‑1 (MQ=255)

                   cGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAACACGGCGTCGATTcc                                                                      >  1:3506235/1‑93 (MQ=255)

                     aTCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGa                                                                    <  2:2071437/93‑1 (MQ=255)

                     aTCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGa                                                                    >  1:3316840/1‑93 (MQ=255)

                      tCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGAt                                                                   <  2:712493/93‑1 (MQ=255)

                      tCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGAt                                                                   >  1:3133531/1‑93 (MQ=255)

                      tCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGAt                                                                   >  1:164399/1‑93 (MQ=255)

                       cTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGAtt                                                                  >  2:3725787/1‑93 (MQ=255)

                        tGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTa                                                                 >  2:2434780/1‑93 (MQ=255)

                        tGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTa                                                                 >  2:6250260/1‑93 (MQ=255)

                         gCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTaa                                                                >  2:5067252/1‑93 (MQ=255)

                            ggAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAg                                                             <  2:2307727/93‑1 (MQ=255)

                            ggAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAg                                                             >  2:171872/1‑93 (MQ=255)

                            ggAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAg                                                             >  2:5849426/1‑93 (MQ=255)

                            ggAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAg                                                             >  1:4710720/1‑93 (MQ=255)

                            ggAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGCCGATTCCGATTAACAg                                                             >  2:4828266/1‑93 (MQ=255)

                             gAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGt                                                            <  1:2166674/93‑1 (MQ=255)

                             gAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGt                                                            <  1:1339668/93‑1 (MQ=255)

                              aaaGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTa                                                           <  1:6223432/93‑1 (MQ=255)

                                aGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAAc                                                         <  2:4445377/93‑1 (MQ=255)

                                aGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAAc                                                         >  1:4765904/1‑93 (MQ=255)

                                  cgcAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCt                                                       <  1:4998835/93‑1 (MQ=255)

                                     aGTAGGCGTCTCCTGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGAt                                                    <  2:3666806/93‑1 (MQ=255)

                                     aGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTGGATTCCGATTAACAGTAACCTGAt                                                    >  2:1012081/1‑93 (MQ=255)

                                      gTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGAtt                                                   <  1:5267263/93‑1 (MQ=255)

                                      gTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGAtt                                                   <  2:5418932/93‑1 (MQ=255)

                                        aGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGt                                                 >  1:1791337/1‑93 (MQ=255)

                                         ggCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTc                                                >  2:802741/1‑93 (MQ=255)

                                          gCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTcc                                               <  1:6109728/93‑1 (MQ=255)

                                          gCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTcc                                               <  1:2941362/93‑1 (MQ=255)

                                           cGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCa                                              <  2:1361941/93‑1 (MQ=255)

                                             tctcCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACg                                            >  2:259564/1‑93 (MQ=255)

                                             tctcCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACg                                            >  1:2860796/1‑93 (MQ=255)

                                              ctcCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACTTGATTGTCCACGt                                           >  1:4007668/1‑93 (MQ=255)

                                               tcCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGtt                                          <  2:4706610/93‑1 (MQ=255)

                                               tcCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGtt                                          >  1:5113165/1‑93 (MQ=255)

                                               tcCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGtt                                          >  1:3617057/1‑93 (MQ=255)

                                                ccAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTg                                         >  1:4888785/1‑93 (MQ=255)

                                                     gATGTGGTGATGCTTAAAGATAACCTGCAACCGGAAGTGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGcaac                                    <  1:2296199/93‑1 (MQ=255)

                                                      aTGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGcaaca                                   >  1:911811/1‑93 (MQ=255)

                                                      aTGTGGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGcaaca                                   >  2:1523310/1‑93 (MQ=255)

                                                       tgtgGTGATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGcaacaa                                  <  2:5793052/93‑1 (MQ=255)

                                                        gtggtgATGCTTAACGATAACCTGCAACCGGAAGGTCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAAt                                 <  1:6449307/93‑1 (MQ=255)

                                                        gtggtgATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAAt                                 <  1:1858411/93‑1 (MQ=255)

                                                        gtggtgATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAAt                                 >  1:246991/1‑93 (MQ=255)

                                                        gtggtgATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAAt                                 <  1:1032727/93‑1 (MQ=255)

                                                         tggtgATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAAtg                                <  2:2899349/93‑1 (MQ=255)

                                                         tggtgATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAAtg                                <  1:1605476/93‑1 (MQ=255)

                                                         tggtgATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAAtg                                >  1:4258263/1‑93 (MQ=255)

                                                          ggtgATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGACTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAAtgt                               >  1:1149357/1‑93 (MQ=255)

                                                             gATGCTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTgcg                            >  2:6466271/1‑93 (MQ=255)

                                                                 cTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCAtt                        <  1:1918024/93‑1 (MQ=255)

                                                                 cTTAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCAtt                        <  2:5176758/93‑1 (MQ=255)

                                                                  ttAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTg                       <  1:4156073/93‑1 (MQ=255)

                                                                  ttAACGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTg                       >  1:1219682/1‑93 (MQ=255)

                                                                    aaCGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGAc                     >  2:2797823/1‑93 (MQ=255)

                                                                    aaCGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGAc                     <  2:5551552/93‑1 (MQ=255)

                                                                      cGATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGc                   <  2:5024155/93‑1 (MQ=255)

                                                                       gATAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGTATTGACGcc                  >  2:407575/1‑93 (MQ=255)

                                                                        aTAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCt                 <  1:6440862/93‑1 (MQ=255)

                                                                        aTAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCt                 <  1:2616529/93‑1 (MQ=255)

                                                                         tAACCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCtt                >  2:5967076/1‑93 (MQ=255)

                                                                         caaCCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCtt                <  1:2047098/92‑1 (MQ=255)

                                                                           aCCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTg              <  1:2583120/93‑1 (MQ=255)

                                                                           aCCTGCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTg              >  2:5106889/1‑93 (MQ=255)

                                                                           aCCTGCAACCGGAAGCGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTg              <  1:2508593/93‑1 (MQ=255)

                                                                               gCAACCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCt          >  2:5596237/1‑93 (MQ=255)

                                                                                 aaCCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCTGa        <  1:5995255/93‑1 (MQ=255)

                                                                                  aCCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCTGaa       >  1:3697781/1‑93 (MQ=255)

                                                                                  aCCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCTGaa       >  2:3906264/1‑93 (MQ=255)

                                                                                  aCCGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCTGaa       >  2:3296016/1‑93 (MQ=255)

                                                                                   ccGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCTGaaa      >  1:3661588/1‑93 (MQ=255)

                                                                                    cGGAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCTGAAAg     <  2:478444/93‑1 (MQ=255)

                                                                                     ggAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCTGAAAgc    <  1:4463734/93‑1 (MQ=255)

                                                                                     ggAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCTGAAAgc    >  1:1552511/1‑93 (MQ=255)

                                                                                     ggAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCTGAAAgc    >  1:6180911/1‑93 (MQ=255)

                                                                                     ggAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCTGAAAgc    <  2:182541/93‑1 (MQ=255)

                                                                                      gAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCTGAAAgcg   >  2:6089957/1‑93 (MQ=255)

                                                                                      gAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCTGAAAgcg   <  2:2704284/93‑1 (MQ=255)

                                                                                      gAAGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCTGAAAgcg   >  1:1506385/1‑93 (MQ=255)

                                                                                       aaGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCTGAAAgcgc  >  2:5319005/1‑93 (MQ=255)

                                                                                       aaGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCTGAAAgcgc  <  1:2968050/93‑1 (MQ=255)

                                                                                       aaGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCTGAAAgcgc  >  1:5627315/1‑93 (MQ=255)

                                                                                       aaGGGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCTGAAAgccc  >  2:4232597/1‑91 (MQ=255)

GGGCGCGCATCGTGGTGCACGATCTGCCGGAAAGCGCAGTAGGCGTCTCCAGCGATGTGGTGATGCTTAACGATAACCTGCAACCGGAAGAGCCGAAAACGGCGTCGATTCCGATTAACAGTAACCTGATTGTCCACGTTGGCAACAATGTGCGCATTGACGCCTTTGGCCTGAAAGCGC  >  NC_000913/4444651‑4444830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`