BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,542,425	A→G	Y168C (TAC→TGC)	b4316 →	Pil

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,542,425	0	A	G	99.5%	741.2 / ‑4.7	211	Y168C (TAC→TGC)	b4316	Pil
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (101/109); total (101/110)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.45e-01

Only 100 of 215 total aligned reads displayed.

AATTAGCGTTGCCACCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTT  >  NC_000913/4542335‑4542514

aaTTAGCGTTGCCACCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGcc                                                                                         >  1:4029895/1‑93 (MQ=255)

 aTTAGCGTTGCCACCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTACCt                                                                                        <  2:1981683/93‑1 (MQ=255)

  ttAGCGTTGCCACCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTg                                                                                       <  1:1364772/93‑1 (MQ=255)

      cGTTGCCACCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACgg                                                                                   >  2:898955/1‑93 (MQ=255)

      cGTTGCCACCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACgg                                                                                   <  2:1433647/93‑1 (MQ=255)

       gTTGCCACCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGt                                                                                  >  1:1988785/1‑93 (MQ=255)

        ttGCCACCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTa                                                                                 >  1:3666874/1‑93 (MQ=255)

          gCCACCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTacc                                                                               >  2:6065244/1‑91 (MQ=255)

           ccACCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGTCGGTAACa                                                                              <  1:6295989/93‑1 (MQ=255)

           ccACCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACa                                                                              >  1:6054672/1‑93 (MQ=255)

            cACCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACag                                                                             <  1:1091414/93‑1 (MQ=255)

            cACCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACag                                                                             >  2:4060236/1‑93 (MQ=255)

            cACCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACag                                                                             >  2:3764224/1‑93 (MQ=255)

             aCCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACACTGATTAACCCGACACCCTATTGCCTGACGGTAACaga                                                                            >  1:1732598/1‑93 (MQ=255)

              cccGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACagag                                                                           <  2:5243375/93‑1 (MQ=255)

               ccGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGt                                                                          <  1:5054073/93‑1 (MQ=255)

               ccGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGt                                                                          >  2:2189910/1‑93 (MQ=255)

               ccGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGt                                                                          <  2:1886914/93‑1 (MQ=255)

                cGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACCCCCTATTGCCTGACGGTAACAGAGtt                                                                         <  2:2551068/93‑1 (MQ=255)

                cGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGtt                                                                         <  2:3028051/93‑1 (MQ=255)

                cGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGtt                                                                         <  1:6063723/93‑1 (MQ=255)

                cGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGtt                                                                         >  2:2608419/1‑93 (MQ=255)

                 gATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTg                                                                        >  1:3314176/1‑93 (MQ=255)

                  aTCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGa                                                                       >  1:891080/1‑93 (MQ=255)

                  aTCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGa                                                                       >  2:4205284/1‑93 (MQ=255)

                  aTCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGATGa                                                                       >  2:6467555/1‑93 (MQ=255)

                   tCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGCATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACCGAGTTgca                                                                      >  1:4373885/1‑91 (MQ=255)

                   tCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGaa                                                                      <  2:5497707/93‑1 (MQ=255)

                     aGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATg                                                                    >  2:2319762/1‑93 (MQ=255)

                      ggCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACACGACACCCTATTGCCTGACGGTAACAGAGTTGAATGc                                                                   >  2:3403984/1‑93 (MQ=255)

                         cGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCgg                                                                >  2:3586812/1‑93 (MQ=255)

                         cGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCgg                                                                <  1:4648547/93‑1 (MQ=255)

                          gCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGa                                                               >  2:6408468/1‑93 (MQ=255)

                           cAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGaa                                                              >  2:4917447/1‑93 (MQ=255)

                            aGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAAc                                                             >  1:1072427/1‑93 (MQ=255)

                            aGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAAc                                                             >  2:4721318/1‑93 (MQ=255)

                            aGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAAc                                                             <  1:233385/93‑1 (MQ=255)

                              aaaaaTTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAAccc                                                           >  2:4814006/1‑93 (MQ=255)

                              aaaaaTTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAAccc                                                           >  2:1676109/1‑93 (MQ=255)

                                 aaTTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCggg                                                        <  2:1546940/93‑1 (MQ=255)

                                  aTTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGt                                                       <  1:267080/93‑1 (MQ=255)

                                  aTTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGt                                                       <  2:2208391/93‑1 (MQ=255)

                                   ttAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGtt                                                      >  1:473624/1‑93 (MQ=255)

                                     aaGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCt                                                    <  1:5347134/93‑1 (MQ=255)

                                     aaGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCt                                                    >  2:764497/1‑93 (MQ=255)

                                      aGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCtt                                                   <  1:2959443/93‑1 (MQ=255)

                                        aTTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGa                                                 >  1:3171987/1‑93 (MQ=255)

                                         tttCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGaa                                                >  1:453307/1‑93 (MQ=255)

                                           tcgtcgTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGaaaa                                              <  2:4366504/93‑1 (MQ=255)

                                               cgtAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCa                                          >  2:1797869/1‑93 (MQ=255)

                                                gtAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCAt                                         >  1:3945380/1‑93 (MQ=255)

                                                gtAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCAt                                         <  2:556229/93‑1 (MQ=255)

                                                  aGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAAACCGGGTTCTTGAAAATGCATTg                                       >  1:1607099/1‑93 (MQ=255)

                                                   gcgcGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTgg                                      >  1:3636124/1‑93 (MQ=255)

                                                   gcgcGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTgg                                      >  2:4411149/1‑93 (MQ=255)

                                                     gcgAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTg                                    >  2:5175658/1‑93 (MQ=255)

                                                       gAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGTTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGcc                                  <  1:3846257/93‑1 (MQ=255)

                                                       gAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGcc                                  <  1:244275/93‑1 (MQ=255)

                                                       gAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGcc                                  <  2:1291536/93‑1 (MQ=255)

                                                       gAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGcc                                  <  1:4121213/93‑1 (MQ=255)

                                                       gAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGcc                                  >  1:4902108/1‑93 (MQ=255)

                                                        aaTTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCt                                 <  2:1585173/93‑1 (MQ=255)

                                                        aaTTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCt                                 <  2:6128633/93‑1 (MQ=255)

                                                        aaTTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCt                                 >  2:5047759/1‑93 (MQ=255)

                                                         aTTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTc                                <  2:3261884/93‑1 (MQ=255)

                                                          ttCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTcc                               <  1:6150701/93‑1 (MQ=255)

                                                           tctcTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCa                              <  1:5883445/93‑1 (MQ=255)

                                                             tctGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAAt                            <  2:2500468/93‑1 (MQ=255)

                                                             tctGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAAt                            <  1:4243306/93‑1 (MQ=255)

                                                              ctGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATg                           >  1:506410/1‑93 (MQ=255)

                                                               tGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATgg                          >  2:5110419/1‑93 (MQ=255)

                                                               tGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATgg                          <  1:5991439/93‑1 (MQ=255)

                                                                gACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATggg                         >  1:6461439/1‑93 (MQ=255)

                                                                gACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATggg                         <  1:3100243/93‑1 (MQ=255)

                                                                 aCGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGGTCTTGAAAATGCATTGGTGCCTCCAATGGGc                        >  2:4005620/1‑93 (MQ=255)

                                                                  cGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCg                       >  2:294374/1‑93 (MQ=255)

                                                                    cTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGaa                     >  1:666566/1‑93 (MQ=255)

                                                                    cTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGaa                     <  2:4581850/93‑1 (MQ=255)

                                                                     tGATTAACCCGTCACCGTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGaaa                    <  1:2277271/93‑1 (MQ=255)

                                                                       aTTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGGAAGc                  >  2:122380/1‑93 (MQ=255)

                                                                       aTTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGc                  <  1:3554917/93‑1 (MQ=255)

                                                                        ttAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCa                 <  1:560994/93‑1 (MQ=255)

                                                                         tAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCAc                >  1:5425607/1‑93 (MQ=255)

                                                                           aCCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACgg              >  1:225975/1‑93 (MQ=255)

                                                                            cccGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGt             <  1:388493/93‑1 (MQ=255)

                                                                              cGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTa           >  1:2923780/1‑93 (MQ=255)

                                                                              cGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTa           >  2:2686905/1‑93 (MQ=255)

                                                                                acacCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTaaa         <  2:5838656/93‑1 (MQ=255)

                                                                                 cgcccTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAAt        <  2:4787381/91‑1 (MQ=255)

                                                                                 cacCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAAt        >  1:5821748/1‑93 (MQ=255)

                                                                                 cacCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAAt        <  1:1478389/93‑1 (MQ=255)

                                                                                 cacCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAAt        <  1:6415415/93‑1 (MQ=255)

                                                                                 cacCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAAt        <  1:1236233/93‑1 (MQ=255)

                                                                                 cacCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAAt        <  1:2190052/93‑1 (MQ=255)

                                                                                  acCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAAtt       >  2:3445678/1‑93 (MQ=255)

                                                                                   cccTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAATTg      >  1:3371810/1‑93 (MQ=255)

                                                                                     cTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAATTGcc    <  2:3279387/93‑1 (MQ=255)

                                                                                      tATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCt   <  1:4865318/93‑1 (MQ=255)

                                                                                      tATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCt   <  1:447083/93‑1 (MQ=255)

                                                                                       aTTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCtt  <  2:535506/93‑1 (MQ=255)

AATTAGCGTTGCCACCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTT  >  NC_000913/4542335‑4542514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`