Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 556,636 A→G V110A (GTG→GCG)  folD ← 5,10‑methylene‑tetrahydrofolate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913556,6360AG98.0% 628.9 / NA 196V110A (GTG→GCG) folD5,10‑methylene‑tetrahydrofolate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (126/66);  minor base T (0/4);  total (126/70)
Fisher's exact test for biased strand distribution p-value = 1.54e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 248 total aligned reads displayed.

GGACGCAGACGCGGCGCGCGCTGGCACAGACGACCGACGTTGTAAGGATGGAAACCGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCACTTTGACGTTATCAATACCCGCCGGTAACGGCAGTTGAACCAGAATGCCATCGATGGTGTTGTCGGCATTCAGCGTATCGATAAGCTCC  >  NC_000913/556546‑556725
                                                                                          |                                                                                         
ggACGCAGACGCGGCGCGCGCTGGCACAGACTACCGACGTTGTAAGGATGGAAACCGTCCACGTCTTTGTTCGGATGAATACGTTCCGGCGCt                                                                                         >  1:231102/1‑93 (MQ=255)
 gACGCAGACGCGGCGCGCGCTGGCACAGACGACCGACGTTGTAAGGATGGAAACCGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCGCtt                                                                                        <  1:4947985/93‑1 (MQ=255)
 gACGCAGACGCGGCGCGCGCTGGCACAGACGACCGACGTTGTAAGGATGGAAACCGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCGCtt                                                                                        <  1:6433912/93‑1 (MQ=255)
  gcGCAGACGCGGCGCGCGCTGGCACAGACGACCGACGTTGTAAGGATGGAAACCGTCCACGTCTGTGTCCGGATGAATACGTTCCAGCGCttt                                                                                       <  2:159228/92‑1 (MQ=255)
    gCAGACGCGGCGCGCGCTGGCACAGACGACCGACGTTGTAAGGATGGAAACCGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGa                                                                                     <  1:5957162/93‑1 (MQ=255)
      aGACGCGGCGCGCGCTGGCACAGACGACCGACGTTGTAAGGATGGAAACCGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACg                                                                                   >  2:1304825/1‑93 (MQ=255)
      aGACGCGGCGCGCGCTGGCACAGACGACCGACGTTGTAAGGATGGAAACCGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACg                                                                                   >  1:3588880/1‑93 (MQ=255)
      aGACGCGGCGCGCGCTGGCACAGACGACCGACGTTGTAAGGATGGAAACCGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACg                                                                                   >  1:648816/1‑93 (MQ=255)
      aGACGCGGCGCGCGCTGGCACAGACGACCGACGTTGTAAGGATGGAAACCGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACg                                                                                   <  1:2180052/93‑1 (MQ=255)
       gACGCGGCGCGCGCTGGCACAGACGACCGACGTTGTAAGGATGGAAACCGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGt                                                                                  <  1:34443/93‑1 (MQ=255)
       gACGCGGCGCGCGCTGGCACAGACGACCGACGTTGTAAGGATGGAAACCGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGt                                                                                  <  1:5429948/93‑1 (MQ=255)
       gACGCGGCGCGCGCTGGCACAGACGACCGACGTTGTAAGGATGGAAACCGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGt                                                                                  <  1:5663270/93‑1 (MQ=255)
       gACGCGGCGCGCGCTGGCACAGACGACCGACGTTGTAAGGATGGAAACCGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGt                                                                                  <  2:5476285/93‑1 (MQ=255)
        aCGCGGCGCGCGCTGGCACAGACGACCGACGTTGTAAGGATGGAAACCGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGtt                                                                                 <  2:6105967/93‑1 (MQ=255)
           cggcgCGCGCTGGCACAGACGACCGACGTTGTAAGGATGGAAACCGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGTTATc                                                                              <  2:569629/93‑1 (MQ=255)
             gcgcgcgcTGGCACAGACGACCGACGTTGTAAGGATGGAAACCGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGTTATCaa                                                                            >  1:3650511/1‑93 (MQ=255)
             gcgcgcgcTGGCACAGACGACCGACGTTGTAAGGATGGAAACCGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGTTATCaa                                                                            >  1:205188/1‑93 (MQ=255)
             gcgcgcgcTGGCACAGACGACCGACGTTGTAAGGATGGAAACCGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGTTATCaa                                                                            >  2:5048312/1‑93 (MQ=255)
              cgcgcgcTGGCACAGACGACCGACGTTGTAAGGATGGAAACCGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGTTATCAAt                                                                           <  1:3000425/93‑1 (MQ=255)
              cgcgcgcTGGCACAGACGACCGACGTTGTAAGGATGGAAACCGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGTTATCAAt                                                                           <  1:3068250/93‑1 (MQ=255)
               gcgcgcTGGCACAGACGACCGACGTTGTAAGGATGGAAACCGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGTTATCAATa                                                                          <  2:5822985/93‑1 (MQ=255)
                  cgcTGGCACAGACGACCGACGTTGTAAGGATGGAAACCGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGTTATCAATAccc                                                                       <  2:2546752/93‑1 (MQ=255)
                   gcTGGCACAGACGACCGACGTTGTAAGGATGGAAACCGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGTTATCAATACccg                                                                      >  1:257231/1‑93 (MQ=255)
                   gcTGGCACAGACGACCGACGTTGTAAGGATGGAAACCGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGTTATCAATACccg                                                                      >  2:2952949/1‑93 (MQ=255)
                   gcTGGCACAGACGACCGACGTTGTAAGGATGGAAACCGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGTTATCAATACccg                                                                      >  2:604949/1‑93 (MQ=255)
                     tGGCACAGACGCCCGACGTTGTAAGGATGGAAACCGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGTTATCAATACccgcc                                                                    <  2:1113137/93‑1 (MQ=255)
                     tGGCACAGACGACCGACGTTGTAAGGATGGCAACCGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGTTATCAATACccgcc                                                                    >  2:399608/1‑93 (MQ=255)
                      ggCACAGACGACCGACGTTGTAAGGATGGAAACCGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGTTATCAATACccgccg                                                                   <  1:138963/93‑1 (MQ=255)
                      ggCACAGACGACCGACGTTGTAAGGATGGAAACCGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGTTATCAATACccgccg                                                                   >  2:3096592/1‑93 (MQ=255)
                         acaGACGACCGACGTTGTAAGGATGGAAACCGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGTTATCAATACCCGCCGGTa                                                                >  2:4952703/1‑93 (MQ=255)
                          caGACGACCGACGTTGTAAGGATGGAAACCGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGTTATCAATACCCGCCGGTaa                                                               >  2:5513624/1‑93 (MQ=255)
                           aGACGACCGACGTTGTAAGGATGGAAACCGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGTTATCAATACCCGCCGGTAAc                                                              >  2:1990564/1‑93 (MQ=255)
                           aGACGACCGACGTTGTAAGGATGGAAACCGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGTTATCAATACCCGCCGGTAAc                                                              <  1:5029258/93‑1 (MQ=255)
                            gacgacCGACGTTGTAAGGATGGAAACCGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGTTATCAATACCCGCCGGTAACg                                                             <  2:1352986/93‑1 (MQ=255)
                              cgacggacGTTGTAAGGATGAAACCCGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGTTATCAATACCCGCCGGTAACGGc                                                           <  2:6187626/93‑1 (MQ=255)
                              cgaccgacGTTGTAAGGATGGAAACCGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGTTATCAATACCCGCCGGTAACgga                                                           >  2:1494230/1‑92 (MQ=255)
                               gaccgacGTTGTAAGGATGGAAACCGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGTTATCAATACCCGCCGGTAACGGCa                                                          >  2:5164103/1‑93 (MQ=255)
                               gaccgacGTTGTAAGGATGGAAACCGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGTTATCAATACCCGCCGGTAACGGCa                                                          >  2:1967473/1‑93 (MQ=255)
                                 ccgacGTTGTAAGGATGGAAACCGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGTTATCAATACCCGCCGGTAACGGCAGt                                                        >  2:4880161/1‑93 (MQ=255)
                                  cgacGTTGTAAGGATGGAAACCGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGTTATCAATACCCGCCGGTAACGGCAGtt                                                       <  2:6015806/93‑1 (MQ=255)
                                   gacGTTGTAAGGATGGAACCCGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCCCTTTGACGTTATCAATCCCCGCCGGTAACGGCAGTTg                                                      <  2:2827279/93‑1 (MQ=255)
                                   gacGTTGTAAGGATGGAAACCGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGTTATCAATACCCGCCGGTAACGGCAGTTg                                                      >  1:2314584/1‑93 (MQ=255)
                                    acGTTGTAAGGATGGAAACCGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGTTATCAATACCCGCCGGTAACGGCAGTTGa                                                     >  1:3975070/1‑93 (MQ=255)
                                       ttGTAAGGATGGAAACCGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGTTATCAATACCCGCCGGTAACGGCAGTTGAAcc                                                  >  1:1217227/1‑93 (MQ=255)
                                       ttGTAAGGATGGAAACCGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGTTATCAATACCCGCCGGTAACGGCAGTTGAAcc                                                  >  2:3881049/1‑93 (MQ=255)
                                            aGGATGGAAACGTTCCACGTCTTTGTCCGGATGAATCCGTTCCAGCGCTTTGACGTTATCAATACCCGCCGGTAACGGCAGTTGAACCAGAAt                                             <  1:4255358/93‑1 (MQ=255)
                                             ggATGGAAACCGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGTTATCAATACCCGCCGGTAACGGCAGTTGAACCAGAATg                                            >  2:5390762/1‑93 (MQ=255)
                                              gagggAAACCGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCTCTTTGACGTTATCAATCCCCGCCGGTAACGGCAGTTGAACCAGAATGc                                           <  2:3571120/90‑1 (MQ=255)
                                              gATGGAACCCGTCCCCGTCTTTGTCCGGATGAATCCGTTCCAGCGCTTTGACGTTATCAATCCCCGCCGGTAACGGCAGTTGAACCAGAATGc                                           <  2:2665970/93‑1 (MQ=255)
                                               agggAACCCGGCCACGTCTTTGTCCGGATGAATACGTTCCAGCTCTTTGACGTTATCAATACCCGCCGGTAACGGCAGTTGAACCAGAATGcc                                          <  2:361990/91‑1 (MQ=255)
                                                 ggAACCCGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGTTATCAATCCCCGCCGGTAACGGCAGTTGAACCAGAATGCCAt                                        <  1:822438/93‑1 (MQ=255)
                                                  gAAACCGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGTTATCAATACCCGCCGGTAACGGCAGTTGAACCAGAATGCCATc                                       >  1:1685650/1‑93 (MQ=255)
                                                  gAAACCGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGTTATCAATACCCGCCGGTAACGGCAGTTGAACCAGAATGCCATc                                       >  1:3397717/1‑93 (MQ=255)
                                                    aaCCGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGTTATCAATACCCGCCGGTAACGGCAGTTGAACCAGAATGCCATCGa                                     >  1:3231914/1‑93 (MQ=255)
                                                      ccGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGTTATCAATACCCGCCGGTAACGGCAGTTGAACCAGAATGCCATCGATg                                   >  2:2083722/1‑93 (MQ=255)
                                                       cGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCTCTTTGACGTTATCAATCCCCGCCGGTAACGGCAGTTGAACCAGAATGCCATCGATgg                                  <  2:473673/93‑1 (MQ=255)
                                                       cGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGTTATCAATACCCGCCGGTAACGGCAGTTGAACCAGAATGCCATCGATgg                                  >  1:449740/1‑93 (MQ=255)
                                                        gTCCAGTTCTTTGCCCGGATGATTCCGTTCCAGCCCTTGGACTTTATCAATCCCCGCCGGTAACGGCAGTTGAACCAGAATGCCATCGATGgt                                 <  1:874616/93‑1 (MQ=255)
                                                         tCCACGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGTTATCAATCCCCGCCGGTAACGGCAGTTGAACCAGAATGCCATCGATGgtg                                <  1:1926375/93‑1 (MQ=255)
                                                         tCCACGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGTTATCAATACCCGCCGGTAACGGCAGTTGAACCAGAATGCCATCGATGgtg                                >  1:2528623/1‑93 (MQ=255)
                                                         tCCACGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGTTATCAATACCCGCCGGTAACGGCAGTTGAACCAGAATGCCATCGATGgtg                                >  1:4978855/1‑93 (MQ=255)
                                                           cACGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGTTATCAATACCCGCCGGTAACGGCAGTTGAACCAGAATGCCATCGATGGtgtt                              >  2:80014/1‑93 (MQ=255)
                                                           cACGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGTTATCAATACCCGCCGGTAACGGCAGTTGAACCAGAATGCCATCGATGGtgtt                              >  1:1408644/1‑93 (MQ=255)
                                                            gcGTCTCTGTCGGGATGAATGCTTCCCAGCGCTTTGACGTAATAAATCCCCGCCGGTAACGGCAGTTGAACCAGAATGCCATCGATGGtgttg                             <  1:4869968/92‑1 (MQ=255)
                                                            aCGTCTTTGTCCGGATGAATACTTTCCAGCTCTTTGACTTTATCAATACCCGCCGGTAACGGCAGTTGAACCAGAATGCCATCGATGGtgttg                             <  1:1848535/93‑1 (MQ=255)
                                                            aCGTCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGTTATCAATACCCGCCGGTAACGGCAGTTGAACCAGAATGCCATCGATGGtgttg                             >  2:3739787/1‑93 (MQ=255)
                                                              gTCTTTGTCCGGATGAATACGTTCCAGCGTTTTGACGTTATCAATCCCCGCCGGTAACGGCAGTTGAACCAGAATGCCATCGATGGTGTTGTc                           <  1:4088440/93‑1 (MQ=255)
                                                               tCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGTTATCAATCCCCGCCGGTAACGGCAGTTGAACCAGAATGCCATCGATGGTGTTGTCg                          <  1:5228385/93‑1 (MQ=255)
                                                               tCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGTTATCAATACCCGCCGGTAACGGCAGTTGAACCAGAATGCCATCGATGGTGTTGTCg                          >  1:2461457/1‑93 (MQ=255)
                                                               tCTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGTTATCAATACCCGCCGGTAACGGCAGTTGAACCAGAATGCCATCGATGGTGTTGTCg                          >  1:5715357/1‑93 (MQ=255)
                                                               tCTTTGCCCGAATAAATACGTCCCAGCTCTTTGACGTTATCAATCCCCGCCGGTAACGGCAGTTGAACCAGAATGCCATCGATGGTGTTGTCg                          <  1:2652368/93‑1 (MQ=255)
                                                                ttttGTCCGGATGAATACGTTCCAGCGTTTTGACGTTATCAATCCCCGCCGGTAACGGCAGTTGAACCAGAATGCCATCGATGGTGTTGTCgg                         <  2:6388053/92‑1 (MQ=255)
                                                                cTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGTTATCAATACCCGCCGGTAACGGCAGTTGAACCAGAATGCCATCGATGGTGTTGTCgg                         >  2:4424975/1‑93 (MQ=255)
                                                                cTTTGTCCGGATGAATACGTTCCAGCGCTTTGACGTTATCAATACCCGCCGGTAACGGCAGTTGAACCAGAATGACATCGATGGGGTTGTCgg                         >  1:401344/1‑93 (MQ=255)
                                                                 tttGTCCGGATGAATACGTTCCAGCGTTTTGACGTTATAAATACCCGCCGGTAACGGCAGTTGAACCAGAATGCCGTCGATGGTGTTGTCGGc                        <  2:6257523/93‑1 (MQ=255)
                                                                  ttGTCGGGATGAATACGTTCCAGCTCTTTGACGTTATCAATCCCCGCCGGTAACGGCAGTTGAACCAGAATGCCATCGATGGTGTTGTCGGCa                       <  1:1604942/93‑1 (MQ=255)
                                                                     tCCGGATGAATACGTTCCAGCTCTTTGACGTTATCAATACCCGCCGGCAACGGCAGTTGAACCAGAATGCCATCGATGGTGTTGTCGGCATTc                    <  2:4169169/93‑1 (MQ=255)
                                                                     tCCGGATGAATACGTTCCAGCGCTTTGACGTTATCAATACCCGCCGGTAACGGCAGTTGAACCAGAATGCCATCGATGGTGTTGTCGGCATTc                    >  2:718789/1‑93 (MQ=255)
                                                                     tCCGGATGAATACGTTCCAGCGCTTTGACGTTATCAATACCCGCCGGTAACGGCAGTTGAACCAGAATGCCATCGATGGTGTTGTCGGCATTc                    >  1:3183999/1‑93 (MQ=255)
                                                                     cggggATGAATGTGTCCCAGCGTTTGGACGTTATCAATCCCCGCCGGTAACGGCAGTTGAACCAGAATGCCATCGATGGTGTTGTCGGCATTc                    <  1:4416556/90‑1 (MQ=255)
                                                                      ccGGATGAATACGTTCCAGCGCTTTGACGTTATCAATACCCGCCGGTAACGGCAGTTGAACCAGAATGCCATCGATGGTGTTGTCGGCATTCa                   >  1:2431029/1‑93 (MQ=255)
                                                                       cGGATGAATACGTTCCAGCGCTTTGACGTTATCAATACCCGCCGGTAACGGCAGTTGAACCAGAATGCCATCGATGGTGTTGTCGGCATTCAg                  >  2:1856742/1‑93 (MQ=255)
                                                                       cGGATGAATACGTTCCAGCGCTTTGACGTTATCAATACCCGCCGGTAACGGCAGTTGAACCAGAATGCCATCGATGGTGTTGTCGGCATTCAg                  >  2:2053838/1‑93 (MQ=255)
                                                                       cGGATGAATACGTTCCAGCGCTTTGACGTTATCAATACCCGCCGGTAACGGCAGTTGAACCAGAATGCCATCGATGGTGTTGTCGGCATTCAg                  >  1:2675080/1‑93 (MQ=255)
                                                                        ggATGAATACGTTCCAGCGCTTTGACGTTATCAATACCCGCCGGTAACGGCAGTTGAACCAGAATGCCATCGATGGTGTTGTCGGCATTCAGc                 >  2:1019929/1‑93 (MQ=255)
                                                                        ggATGAATACGTTCCAGCGCTTTGACGTTATCAATACCCGCCGGTAACGGCAGTTGAACCAGAATGCCATCGATGGTGTTGTCGGCATTCAGc                 >  1:509629/1‑93 (MQ=255)
                                                                         gATGAATACGTTCCAGCGCTTTGACGTTATCAATACCCGCCGGTAACGGCAGTTGAACCAGAATGCCATCGATGGTGTTGTCGGCATTCAGCg                >  2:3388419/1‑93 (MQ=255)
                                                                         gATGAATACGTTCCAGCGCTTTGACGTTATCAATACCCGCCGGTAACGGCAGTTGAACCAGAATGCCATCGATGGTGTTGTCGGCATTCAGCg                >  2:1664488/1‑93 (MQ=255)
                                                                          ataaaTCCGTTCCAGCTTTTTGACGTTATCAATCCCCGCCGGTAACGGCAGTTGAACCAGAATGCCATCGATGGTGTTGTCGGCATTCAGCGt               <  1:4340581/90‑1 (MQ=255)
                                                                           tGAATACGTTCCAGAGCTTTGACGTTATCAATACCCGCCGGTAACGGCAGTTGAACCAGAATGCCATCGATGGTGTTGTCGGCATTCAGCGTa              >  1:5074599/1‑93 (MQ=255)
                                                                            gAATGCGTCCCAGCGTTTTGACGTTACAAACCCCCGCCGGTAACGGCAGTTGAACCAGAATGCCATCGATGGTGTTGTCGGCATTCAGCGTAt             <  1:6150036/93‑1 (MQ=255)
                                                                            gAATACGTTCCAGCGCTTTGACGTTATCAATACCCGCCGGTAACGGCAGTTGAACCAGAATGCCATCGATGGTGTTGTCGGCATTCAGCGTAt             >  2:2847194/1‑93 (MQ=255)
                                                                             aaTACGTCCCAGCTTTTTGACGTTATAAATCCCCGCCGGTAACGGCAGTTGAACCAGAATGCCATCGATGGTGTTGTCGGCATTCAGCGTATc            <  1:995590/93‑1 (MQ=255)
                                                                                aCGTTCCAGCTTTTTGACGTTATCAATACCCGCCGGTAACGGCAGTTGAACCAGAATGCCATCGATGGTGTTGTCGGCATTCAGCGTATCGAt         <  1:4275932/93‑1 (MQ=255)
                                                                                aCGTTCCAGCGCTTTGACGTTATCAATACCCGCCGGTAACGGCAGTTGAACCAGAATGCCATCGATGGTGTTGTCGGCATTCAGCGTATCGAt         >  2:2358065/1‑93 (MQ=255)
                                                                                 cGTTCCAGCGCTTTGACGTTATCAATACCCGCCGGTAACGGCAGTTGAACCAGAATGCCATCGATGGTGTTGTCGGCATTCCGCGTATCGCTa        >  2:458215/1‑93 (MQ=255)
                                                                                  gTTCCAGCGCTTTGACGTTATCAATACCCGCCGGTAACGGCAGTTGAACCAGAATGCCATCGATGGTGTTGTCGGCATTCAGCGTATCGATaa       >  1:458675/1‑93 (MQ=255)
                                                                                    tCCAGCTTTTTGACTTTATCAATCCCCGCCGGTAACGGCAGTTGAACCAGAATGCCATCGATGGTGTTGTCGGCATTCAGCGTATCGATAAGc     <  1:6335641/93‑1 (MQ=255)
                                                                                       aGCGCTTTGACTTTATCAATCCCCGCCGGTAACGGCAGTTGAACCAGAATGCCATCGATGGTGTTGTCGGCATTCAGCGTATCGATAAGCTcc  <  2:5359270/93‑1 (MQ=255)
                                                                                       aGCGCTTTGACGTTATCAATCCCCGCCGGTAACGGCAGTTGAACCAGAATGCCATCGATGGTGTTGTCGGCATTCAGCGTATCGATAAGCTcc  <  2:2993692/93‑1 (MQ=255)
                                                                                          |                                                                                         
GGACGCAGACGCGGCGCGCGCTGGCACAGACGACCGACGTTGTAAGGATGGAAACCGTCCACGTCTTTGTCCGGATGAATACGTTCCAGCACTTTGACGTTATCAATACCCGCCGGTAACGGCAGTTGAACCAGAATGCCATCGATGGTGTTGTCGGCATTCAGCGTATCGATAAGCTCC  >  NC_000913/556546‑556725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑