BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BA000036	671,707	Δ1 bp	coding (8/957 nt)	Cgl0657 →	Uncharacterized protein involved in propionate catabolism

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BA000036	671,706	0	A	.	99.4%	880.3 / ‑2.1	171	coding (7/957 nt)	Cgl0657	Uncharacterized protein involved in propionate catabolism
Reads supporting (aligned to +/- strand): ref base A (0/1); new base . (88/82); total (88/83)
Fisher's exact test for biased strand distribution p-value = 4.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 174 total aligned reads displayed.

GATTTCCATGACACGTTCCTCGCTGCGGAATACTCCCACCCAGGAGATAACATTCCTCCGATTTTGGCTGCAGCACAGCAGGCTGGAAAAGGTGGCAAGGATCTGATCCGTGGCATCGCTACTGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGGAACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGATGTCCATCGAGGCAGTAGATCGCGCAATGCGTGGCGAGGGCGCACCGTCACCAATCTGGGAAGGCGAAGACGGCGTAATCGCGTGGCTGCTGTCCGGTCTTGATCACATCTACACCATTCCTTTGCCT  >  BA000036/671459‑671948

 aTTTCCATGACACGTTCCTCGCTGCGGAATACTCCCACCCAGGAGATAACATTCCTCCGATTTTGGCTGCAGCACAGCAGGCTGGAAAAGGTGGCAAGGATCTGATCCGTGGCATCGCTACTGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGGaca                                                                                                                                                                                                                                                  <  1:283472/249‑3 (MQ=255)

  tttCCATGACACGTTCCTCGCTGCGGAATACTCCCACCCAGGAGATAACATTCCTCCGATTTTGGCTGCAGCACAGCAGGCTGGAAAAGGTGGCAAGGATCTGATCCGTGGCATCGCTACTGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGGacac                                                                                                                                                                                                                                                 <  2:283473/249‑4 (MQ=255)

     ccATGACACGTTCCTCGCTGCGGAATACTCCCACCCAGGAGATAACATTCCTCCGATTTTGGCTGCAGCACAGCAGGCTGGAAAAGGTGGCAAGGATCTGATCCGTGGCATCGCTACTGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCAt                                                                                                                                                                                                                                             <  1:283474/249‑1 (MQ=255)

      cATGACACGTTCCTCGCTGCGGAATACTCCCACCCAGGAGATAACATTCCTCCGATTTTGGCTGCAGCACAGCAGGCTGGAAAAGGTGGCAAGGATCTGATCCGTGGCATCGCTACTGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATc                                                                                                                                                                                                                                            <  1:283476/249‑1 (MQ=255)

        tGACACGTTCCTCGCTGCGGAATACTCCCACCCAGGAGATAACATTCCTCCGATTTTGGCTGCAGCACAGCAGGCTGGAAAAGGTGGCAAGGATCTGATCCGTGGCATCGCTACTGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTa                                                                                                                                                                                                                                          <  1:283478/249‑1 (MQ=255)

           cacGTTCCTCGCTGCGGAATACTCCCACCCAGGAGATAACATTCCTCCGATTTTGGCTGCAGCACAGCAGGCTGGAAAAGGTGGCAAGGATCTGATCCGTGGCATCGCTACTGGGTATGAGTTTCCGGTTAACTTGGTGCGT‑GAATGTGCCTGCATGAGCACAAGACTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAg                                                                                                                                                                                                                                      <  2:283479/249‑1 (MQ=255)

            acGTTCCTCGCTGCGGAATACTCCCACCCAGGAGATAACATTCCTCCGATTTTGGCTGCAGCACAGCAGGCTGGAAAAGGTGGCAAGGATCTGATCCGTGGCATCGCTACTGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAg                                                                                                                                                                                                                                      <  1:283480/249‑1 (MQ=255)

               ttCCTCGCTGCGGAATACTCCCACCCAGGAGATAACATTCCTCCGATTTTGGCTGCAGCACAGCAGGCTGGAAAAGGTGGCAAGGATCTGATCCGTGGCATCGCTACTGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCa                                                                                                                                                                                                                                   <  1:283483/249‑1 (MQ=255)

                 ccTCGCTGCGGAATACTCCCACCCAGGAGATAACATTCCTCCGATTTTGGCTGCAGCACAGCAGGCTGGAAAAGGTGGCAAGGATCTGATCCGTGGCATCGCTACTGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAAt                                                                                                                                                                                                                                 <  1:283485/249‑1 (MQ=255)

                  cTCGCTGCGGAATACTCCCACCCAGGAGATAACATTCCTCCGATTTTGGCTGCAGCACAGCAGGCTGGAAAAGGTGGCAAGGATCTGATCCGTGGCATCGCTACTGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAAtt                                                                                                                                                                                                                                <  2:283486/249‑1 (MQ=255)

                         cGGAATACTCCCACCCAGGAGATAACATTCCTCCGATTTTGGCTGCAGCACAGCAGGCTGGAAAAGGTGGCAAGGATCTGATCCGTGGCATCGCTACTGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAgg                                                                                                                                                                                                                         >  2:283562/1‑249 (MQ=255)

                             aTACTCCCACCCAGGAGATAACATTCCTCCGATTTTGGCTGCAGCACAGCAGGCTGGAAAAGGTGGCAAGGATCTGATCCGTGGCATCGCTACTGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCAtt                                                                                                                                                                                                                     <  1:283489/249‑1 (MQ=255)

                              tACTCCCACCCAGGAGATAACATTCCTCCGATTTTGGCTGCAGCACAGCAGGCTGGAAAAGGTGGCAAGGATCTGATCCGTGGCATCGCTACTGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTg                                                                                                                                                                                                                    <  2:283490/249‑1 (MQ=255)

                                 tCCCACCCAGGAGATAACATTCCTCCGATTTTGGCTGCAGCACAGCAGGCTGGAAAAGGTGGCAAGGATCTGATCCGTGGCATCGCTACTGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGcac                                                                                                                                                                                                                 >  1:283588/1‑249 (MQ=255)

                                    cacccaGGAGATAACATTCCTCCGATTTTGGCTGCAGCACAGCAGGCTGGAAAAGGTGGCAAGGATCTGATCCGTGGCATCGCTACTGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCAcacc                                                                                                                                                                                                              >  1:283537/1‑249 (MQ=255)

                                        caGGAGATAACATTCCTCCGATTTTGGCTGCAGCACAGCAGGCTGGAAAAGGTGGCAAGGATCTGATCCGTGGCATCGCTACTGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCAcaccacca                                                                                                                                                                                                          >  2:283590/1‑249 (MQ=255)

                                               tAACATTCCTCCGATTTTGGCTGCAGCACAGCAGGCTGGAAAAGGTGGCAAGGATCTGATCCGTGGCATCGCTACTGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGcgac                                                                                                                                                                                                   >  2:283600/1‑249 (MQ=255)

                                               tAACATTCCTCCGATTTTGGCTGCAGCACAGCAGGCTGGAAAAGGTGGCAAGGATCTGATCCGTGGCATCGCTACTGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGcgac                                                                                                                                                                                                   <  2:283492/249‑1 (MQ=255)

                                                aaCATTCCTCCGATTTTGGCTGCAGCACAGCAGGCTGGAAAAGGTGGCAAGGATCTGATCCGTGGCATCGCTACTGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGcgacg                                                                                                                                                                                                  <  1:283494/249‑1 (MQ=255)

                                                aaCATTCCTCCGATTTTGGCTGCAGAACAGCAGGCTGGAAAAGGTGGCAAGGATCTGATCCGTGGCATCGCTACTGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGcgacg                                                                                                                                                                                                  <  2:283493/249‑1 (MQ=255)

                                                  cATTCCTCCGATTTTGGCTGCAGCACAGCAGGCTGGAAAAGGTGGCAAGGATCTGATCCGTGGCATCGCTACTGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAg                                                                                                                                                                                                >  2:283566/1‑249 (MQ=255)

                                                    ttCCTCCGATTTTGGCTGCAGCACAGCAGGCTGGAAAAGGTGGCAAGGATCTGATCCGTGGCATCGCTACTGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGc                                                                                                                                                                                              >  2:283576/1‑249 (MQ=255)

                                                     tcctccGATTTTGGCTGCAGCACAGCAGGCTGGAAAAGGTGGCAAGGATCTGATCCGTGGCATCGCTACTGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGcn                                                                                                                                                                                             >  1:283558/1‑248 (MQ=255)

                                                       ctccGATTTTGGCTGCAGCACAGCAGGCTGGAAAAGGTGGCAAGGATCTGATCCGTGGCATCGCTACTGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGt                                                                                                                                                                                           >  1:283565/1‑249 (MQ=255)

                                                       ctccGATTTTGGCTGCAGCACAGCAGGCTGGAAAAGGTGGCAAGGATCTGATCCGTGGCATCGCTACTGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGt                                                                                                                                                                                           <  1:283497/249‑1 (MQ=255)

                                                         ccGATTTTGGCTGCAGCACAGCAGGCTGGAAAAGGTGGCAAGGATCTGATCCGTGGCATCGCTACTGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTcc                                                                                                                                                                                         <  2:283498/249‑1 (MQ=255)

                                                               ttGGCTGCAGCACAGCAGGCTGGAAAAGGTGGCAAGGATCTGATCCGTGGCATCGCTACTGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTaaa                                                                                                                                                                                   <  1:283501/249‑1 (MQ=255)

                                                                 ggttGCAGCACAGCAGGCTGGAAAAGGTGGCAAGGATCTGATCCGTGGCATCGCTACTGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGACCCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAgg                                                                                                                                                                                 <  2:283503/246‑1 (MQ=255)

                                                                    tGCAGCACAGCAGGCTGGAAAAGGTGGCAAGGATCTGATCCGTGGCATCGCTACTGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGc                                                                                                                                                                              >  1:283663/1‑249 (MQ=255)

                                                                      cagcacagcaGGCTGGAAAAGGTGGCAAGGATCTGATCCGTGGCATCGCTACTGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGa                                                                                                                                                                            <  2:283505/249‑1 (MQ=255)

                                                                      cagcacagcaGGCTGGAAAAGGTGGCAAGGATCTGATCCGTGGCATCGCTACTGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGa                                                                                                                                                                            <  1:283504/249‑1 (MQ=255)

                                                                            agcagGCTGGAAAAGGTGGCAAGGATCTGATCCGTGGCATCGCTACTGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATttctt                                                                                                                                                                      <  1:283506/249‑1 (MQ=255)

                                                                             gcagGCTGGAAAAGGTGGCAAGGATCTGATCCGTGGCATCGCTACTGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATttcttc                                                                                                                                                                     <  2:283508/249‑1 (MQ=255)

                                                                                ggCTGGAAAAGGTGGCAAGGATCTGATCCGTGGCATCGCTACTGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATg                                                                                                                                                                  >  1:283637/1‑249 (MQ=255)

                                                                                 gCTGGAAAAGGTGGCAAGGATCTGATCCGTGGCATCGCTACTGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATgg                                                                                                                                                                 >  2:283594/1‑249 (MQ=255)

                                                                                 gCTGGAAAAGGTGGCAAGGATCTGATCCGTGGCATCGCTACTGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATgg                                                                                                                                                                 >  1:283691/1‑249 (MQ=255)

                                                                                    ggAAAAGGTGGCAAGGATCTGATCCGTGGCATCGCTACTGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAg                                                                                                                                                              <  1:283511/249‑1 (MQ=255)

                                                                                        aaGGTGGCAAGGATCTGATCCGTGGCATCGCTACTGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCAt                                                                                                                                                          >  1:283732/1‑249 (MQ=255)

                                                                                          ggTGGCAAGGATCTGATCCGTGGCATCGCTACTGGGTATGAGATTCAGGTTAACTTTGTGCGTGGAATGTGCATGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCAttt                                                                                                                                                        <  2:283512/249‑1 (MQ=255)

                                                                                             ggCAAGGATCTGATCCGTGGCATCGCTACTGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCt                                                                                                                                                     >  1:283607/1‑249 (MQ=255)

                                                                                                  ggATCTGATCCGTGGCATCGCTACTGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGc                                                                                                                                                >  2:283636/1‑249 (MQ=255)

                                                                                                   gATCTGATCCGTGGCATCGCTACTGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCg                                                                                                                                               <  1:283515/249‑1 (MQ=255)

                                                                                                     tCTGATCCGTGGCATCGCTACTGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGtt                                                                                                                                             >  2:283666/1‑249 (MQ=255)

                                                                                                     nnngATCCGTGGCATCGCTACTGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGtt                                                                                                                                             <  2:283516/246‑1 (MQ=255)

                                                                                                          tCCGTGGCATCGCTACTGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCgg                                                                                                                                        <  2:283517/249‑1 (MQ=255)

                                                                                                          tCCGTGGCATCGCTACTGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCgg                                                                                                                                        >  2:283612/1‑249 (MQ=255)

                                                                                                             gTGGCATCGCTACTGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCa                                                                                                                                     >  2:283670/1‑249 (MQ=255)

                                                                                                                 cATCGCTACTGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCTCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGAt                                                                                                                                 <  2:283520/249‑1 (MQ=255)

                                                                                                                       naCTGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGATGTCCAt                                                                                                                           <  2:283522/248‑1 (MQ=255)

                                                                                                                        aCTGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGATGTCCATc                                                                                                                          <  2:283524/249‑1 (MQ=255)

                                                                                                                         cTGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGATGTCCATCg                                                                                                                         >  1:283669/1‑249 (MQ=255)

                                                                                                                         cTGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCGGCATGATCACAAGATTGATCACGTTGCCCATATTGGACCATCAGCGGCTGCTGGTAGCGGATGCTTGCGAGACCTAGATGT‑GAACACCAGCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCTATTGCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGATGTCCTTCg                                                                                                                         <  2:283525/249‑1 (MQ=255)

                                                                                                                          tGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGATGTCCATCgn                                                                                                                        >  1:283606/1‑248 (MQ=255)

                                                                                                                            ggTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGATGTCCATCGAgg                                                                                                                      <  1:283526/249‑1 (MQ=255)

                                                                                                                            ggTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGATGTCCATCGAgg                                                                                                                      >  2:283579/1‑249 (MQ=255)

                                                                                                                               ttttgaTTCAGGTGAACTTGGTGCGTGGAATGTGTCGACATGAGCACAATATTGATCACGTTGCCAATCTTTTACCATCAGCGGCTTTTATTATCGGAACCTTGCTCGTCATAGGTTTGG‑ACACCATCTTCCATGCAATTGGTCAGGCATTGTACACCACCACGGCGATGAGGCAGCCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGAGCAAGTTGTCCATCGAGGCAg                                                                                                                   <  2:283529/245‑1 (MQ=255)

                                                                                                                                tGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGATGTCCATCGAGGCAGt                                                                                                                  >  2:283692/1‑249 (MQ=255)

                                                                                                                                 gagaTTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGATGTCCATCGAGGCAGTa                                                                                                                 <  1:283527/249‑1 (MQ=255)

                                                                                                                                   gaTTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGATGTCCATCGAGGCAGtnnn                                                                                                               >  2:283610/1‑246 (MQ=255)

                                                                                                                                       cAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGATGTCCATCGAGGCAGTAGATcgc                                                                                                           >  2:283633/1‑249 (MQ=255)

                                                                                                                                         ggTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGATGTCCATCGAGGCAGTAGATCgcgc                                                                                                         >  2:283605/1‑249 (MQ=255)

                                                                                                                                            tAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGATGTCCATCGAGGCAGTAGATCGCGCAAt                                                                                                      >  1:283627/1‑249 (MQ=255)

                                                                                                                                              aCTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGATGTCCATCGAGGCAGTAGATCGCGCAATGc                                                                                                    <  2:283531/249‑1 (MQ=255)

                                                                                                                                                 tGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGATGTCCATCGAGGCAGTAGATCGCGCAATGCGTg                                                                                                 >  2:283654/1‑249 (MQ=255)

                                                                                                                                                 tGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGATGTCCATCGAGGCAGTAGATCGCGCAATGCGTg                                                                                                 <  1:283532/249‑1 (MQ=255)

                                                                                                                                                    tGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCCCCTGCGTTTGCGGGCAAGATGTCCATCGAGGCAGTAGATCGCGCAATGCGTGGCg                                                                                              >  2:283617/1‑249 (MQ=255)

                                                                                                                                                       gTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGATGTCCATCGAGGCAGTAGATCGCGCAATGCGTGGCGAgg                                                                                           <  1:283535/249‑1 (MQ=255)

                                                                                                                                                           aaTGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGATGTCCATCGAGGCAGTAGATCGCGCAATGCGTGGCGAGGgcgc                                                                                       >  2:283630/1‑249 (MQ=255)

                                                                                                                                                            aTGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGATGTCCATCGAGGCAGTAGATCGCGCAATGCGTGGCGAGGGCGCa                                                                                      >  2:283659/1‑249 (MQ=255)

                                                                                                                                                               tgCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGATGTCCATCGAGGCAGTAGATCGCGCAATGCGTGGCGAGGGCGCACCg                                                                                   <  2:283537/249‑1 (MQ=255)

                                                                                                                                                               tgCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGATGTCCATCGAGGCAGTAGATCGCGCAATGCGTGGCGAGGGCGCACCg                                                                                   >  2:283743/1‑249 (MQ=255)

                                                                                                                                                               tgCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGATGTCCATCGAGGCAGTAGATCGCGCAATGCGTGGCGAGGGCGCACCg                                                                                   >  2:283629/1‑249 (MQ=255)

                                                                                                                                                                   ngCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGATGTCCATCGAGGCAGTAGATCGCGCAATGCGTGGCGAGGGCGCACCGTCAc                                                                               <  1:283538/248‑1 (MQ=255)

                                                                                                                                                                      aTGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGATGTCCATCGAGGCAGTAGATCGCGCAATGCGTGGCGAGGGCGCACCGTCACCaa                                                                            >  1:283598/1‑249 (MQ=255)

                                                                                                                                                                       tGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGATGTCCATCGAGGCAGTAGATCGCGCAATGCGTGGCGAGGGCGCACCGTCACCAAt                                                                           <  2:283539/249‑1 (MQ=255)

                                                                                                                                                                         aGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGATGTCCATCGAGGCAGTAGATCGCGCAATGCGTGGCGAGGGCGCACCGTCACCAATCt                                                                         >  2:283593/1‑249 (MQ=255)

                                                                                                                                                                         aGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGATGTCCATCGAGGCAGTAGATCGCGCAATGCGTGGCGAGGGCGCACCGTCACCAATCt                                                                         >  2:283624/1‑249 (MQ=255)

                                                                                                                                                                            acaAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGATGTCCATCGAGGCAGTAGATCGCGCAATGCGTGGCGAGGGCGCACCGTCACCAATCTggg                                                                      <  2:283542/249‑1 (MQ=255)

                                                                                                                                                                            acaAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGATGTCCATCGAGGCAGTAGATCGCGCAATGCGTGGCGAGGGCGCACCGTCACCAATCTggg                                                                      <  1:283543/249‑1 (MQ=255)

                                                                                                                                                                                       cACGGTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGATGTCCATCGAGGCAGTAGATCGCGCAATGCGTGGCGAGGGCGCACCGTCACCAATCTGGGAAGGCGAAGAc                                                           <  1:283546/249‑1 (MQ=255)

                                                                                                                                                                                        aCGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGATGTCCATCGAGGCAGTAGATCGCGCAATGCGTGGCGAGGGCGCACCGTCACCAATCTGGGAAGGCGAAGACg                                                          <  2:283547/249‑1 (MQ=255)

                                                                                                                                                                                              cTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGATGTCCATCGAGGCAGTAGATCGCGCAATGCGTGGCGAGGGCGCACCGTCACCAATCTGGGCAGGCGAAGACGGCGTaa                                                    >  1:283621/1‑249 (MQ=255)

                                                                                                                                                                                                           aTCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGATGTCCATCGAGGCAGTAGATCGCGCAATGCGTGGCGAGGGCGCACCGTCACCAATCTGGGAAGGCGAAGACGGCGTAATCGCGTGgctgct                                       <  1:283549/249‑1 (MQ=255)

                                                                                                                                                                                                           aTCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGATGTCCATCGAGGCAGTAGATCGCGCAATGCGTGGCGAGGGCGCACCGTCACCAATCTGGGAAGGCGAAGACGGCGTAATCGCGTGgctgct                                       <  1:283550/249‑1 (MQ=255)

                                                                                                                                                                                                               gCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGATGTCCATCGAGGCAGTAGATCGCGCAATGCGTGGCGAGGGCGCACCGTCACCAATCTGGGAAGGCGAAGACGGCGTAATCGCGTGGCTGCTGTcc                                   <  2:283551/249‑1 (MQ=255)

                                                                                                                                                                                                               gCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGATGTCCATCGAGGCAGTAGATCGCGCAATGCGTGGCGAGGGCGCACCGTCACCAATCTGGGAAGGCGAAGACGGCGTAATCGCGTGGCTGCTGTcc                                   >  2:283779/1‑249 (MQ=255)

                                                                                                                                                                                                                 ggCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGATGTCCATCGAGGCAGTAGATCGCGCAATGCGTGGCGAGGGCGCACCGTCACCAATCTGGGAAGGCGAAGACGGCGTAATCGCGTGGCTGCTGTCCgg                                 >  1:283673/1‑249 (MQ=255)

                                                                                                                                                                                                                     gctgGTATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGATGTCCATCGAGGCAGTAGATCGCGCAATGCGTGGCGAGGGCGCACCGTCACCAATCTGGGAAGGCGAAGACGGCGTAATCGCGTGGCTGCTGTCCGGTCtt                             >  2:283765/1‑249 (MQ=255)

                                                                                                                                                                                                                          tATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGATGTCCATCGAGGCAGTAGATCGCGCAATGCGTGGCGAGGGCGCACCGTCACCAATCTGGGAAGGCGAAGACGGCGTAATCGCGTGGCTGCTGTCCGGTCTTGATca                        >  1:283603/1‑249 (MQ=255)

                                                                                                                                                                                                                          tATCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGATGTCCATCGAGGCAGTAGATCGCGCAATGCGTGGCGAGGGCGCACCGTCACCAATCTGGGAAGGCGAAGACGGCGTAATCGCGTGGCTGCTGTCCGGTCTTGATca                        >  1:283581/1‑249 (MQ=255)

                                                                                                                                                                                                                           aTCGGAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGATGTCCATCGAGGCAGTAGATCGCGCAATGCGTGGCGAGGGCGCACCGTCACCAATCTGGGAAGGCGAAGACGGCGTAATCGCGTGGCTGCTGTCCGGTCTTGATcac                       >  1:283585/1‑249 (MQ=255)

                                                                                                                                                                                                                              ggAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGATGTCCATCGAGGCAGTAGATCGCGCAATGCGTGGCGAGGGCGCACCGTCACCAATCTGGGAAGGCGAAGACGGCGTAATCGCGTGGCTGCTGTCCGGTCTTGATCACATc                    >  2:283648/1‑249 (MQ=255)

                                                                                                                                                                                                                               gAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGATGTCCATCGAGGCAGTAGATCGCGCAATGCGTGGCGAGGGCGCACCGTCACCAATCTGGGAAGGCGAAGACGGCGTAATCGCGTGGCTGCTGTCCGGTCTTGATCACATCt                   >  2:283701/1‑249 (MQ=255)

                                                                                                                                                                                                                               gAACCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGATGTCCATCGAGGCAGTAGATCGCGCAATGCGTGGCGAGGGCGCACCGTCACCAATCTGGGAAGGCGAAGACGGCGTAATCGCGTGGCTGCTGTCCGGTCTTGATCACATCt                   >  2:283750/1‑249 (MQ=255)

                                                                                                                                                                                                                                aaCCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGATGTCCATCGAGGCAGTAGATCGCGCAATGCGTGGCGAGGGCGCACCGTCACCAATCTGGGAAGGCGAAGACGGCGTAATCGCGTGGCTGCTGTCCGGTCTTGATCACATCTa                  <  1:283554/249‑1 (MQ=255)

                                                                                                                                                                                                                                aaCCTTGCTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGATGTCCATCGAGGCAGTAGATCGCGCAATGCGTGGCGAGGGCGCACCGTCACCAATCTGGGAAGGCGAAGACGGCGTAATCGCGTGGCTGCTGTCCGGTCTTGATCACATCTa                  <  2:283553/249‑1 (MQ=255)

                                                                                                                                                                                                                                       cTAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGATGTCCATCGAGGCAGTAGATCGCGCAATGCGTGGCGAGGGCGCACCGTCACCAATCTGGGAAGGCGAAGACGGCGTAATCGCGTGGCTGCTGTCCGGTCTTGATCACATCTACACCAtt           >  2:283679/1‑249 (MQ=255)

                                                                                                                                                                                                                                        tAGACCTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGATGTCCATCGAGGCAGTAGATCGCGCAATGCGTGGCGAGGGCGCACCGTCACCAATCTGGGAAGGCGAAGACGGCGTAATCGCGTGGCTGCTGTCCGGTCTTGATCACATCTACACCATTc          >  1:283700/1‑249 (MQ=255)

                                                                                                                                                                                                                                            ccTAGATGTGG‑ACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGATGTCCATCGAGGCAGTAGATCGCGCAATGCGTGGCGAGGGCGCACCGTCACCAATCTGGGAAGGCGAAGACGGCGTAATCGCGTGGCTGCTGTCCGGTCTTGATCACATCTACACCATTCCttt      >  1:283715/1‑249 (MQ=255)

                                                                                                                                                                                                                                                gATGTGG‑ACACCATCTACCAGGCGATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGATGTCCATCGAGGCAGTAGATCGCGCAATGCGTGGCGAGGGCGCACCGTCACCAATCTGGGAAGGCGAAGACGGCGTAATCGCGTGGCTGCTGTCCGGTCTTGATCACATCTACACCATTCCTTTGCCt  <  1:283556/249‑1 (MQ=255)

GATTTCCATGACACGTTCCTCGCTGCGGAATACTCCCACCCAGGAGATAACATTCCTCCGATTTTGGCTGCAGCACAGCAGGCTGGAAAAGGTGGCAAGGATCTGATCCGTGGCATCGCTACTGGGTATGAGATTCAGGTTAACTTGGTGCGTGGAATGTGCCTGCATGAGCACAAGATTGATCACGTTGCTCATCTTGGACCATCAGCGGCTGCTGGTATCGGAACCTTGCTAGACCTAGATGTGGAACACCATCTACCAGGCAATTGGTCAGGCATTGCACACCACCACGGCGACGAGGCAGTCCCGTAAAGGTGCGATTTCTTCATGGAAGGCATTTGCTCCTGCGTTTGCGGGCAAGATGTCCATCGAGGCAGTAGATCGCGCAATGCGTGGCGAGGGCGCACCGTCACCAATCTGGGAAGGCGAAGACGGCGTAATCGCGTGGCTGCTGTCCGGTCTTGATCACATCTACACCATTCCTTTGCCT  >  BA000036/671459‑671948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`