BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BA000036	3,155,328	T→C	Q457Q (CAA→CAG)	Cgl2951 ←	Predicted transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BA000036	3,155,328	0	T	C	99.5%	788.4 / NA	198	Q457Q (CAA→CAG)	Cgl2951	Predicted transposase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (94/103); minor base A (1/0); total (95/103)
Fisher's exact test for biased strand distribution p-value = 4.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 198 total aligned reads displayed.

CAAGGGGGTCCAATCCTAAAACCGGACTAAAACCAGACTGTAAAGTGTCAGCACGTTGTGCACACTCAATATCATATCCTTGCGCACGCCCGATCCCACTAGGTTGTGCACACACAAAGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGTTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAACCAAGCTCAGTTGCCGTCACCGTCCGATAAAACTCCCTGTCCCTCATCGTACGAGGAACAGAAATACGCATCCCCTGGAACACCACCTTCTGATTATTCCCAGCAAACGTAAACACATCAGGATCCACCG  >  BA000036/3155082‑3155572

cAAGGGGGTCCAATCCTAAAACCGGACTAAAACCAGACTGTAAAGTGTCAGCACGTTGTGCACACTCAATATCATATCCTTGCGCACGCCCGATCCCACTAGGTTGTGCACACACAAAGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTg                                                                                                                                                                                                                                                    <  1:1328049/249‑1 (MQ=255)

cAAGGGGGTCCAATCCTAAAACCGGACTAAAACCAGACTGTAAAGTGTCAGCACGTTGTGCACACTCAATATCATATCCTTGCGCACGCCCGATCCCACTAGGTTGTGCACACACAAAGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTg                                                                                                                                                                                                                                                    >  2:1328104/1‑249 (MQ=255)

     gggTCCAATCCTAAAACCGGACTAAAACCAGACTGTAAAGTGTCAGCACGTTGTGCACACTCAATATCATATCCTTGCGCACGCCCGATCCCACTAGGTTGTGCACACACAAAGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATcc                                                                                                                                                                                                                                               <  1:1328050/249‑1 (MQ=255)

      ggTCCAATCCTAAAACCGGACTAAAACCAGACTGTAAAGTGTCAGCACGTTGTGCACACTCAATATCATATCCTTGCGCACGCCCGATCCCACTAGGTTGTGCACACACAAAGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCca                                                                                                                                                                                                                                              >  2:1328118/1‑249 (MQ=255)

          cAATCCTAAAACCGGACTAAAACCAGACTGTAAAGTGTCAGCACGTTGTGCACACTCAATATCATATCCTTGCGCACGCCCGATCCCACTAGGTTGTGCACACACAAAGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACAcc                                                                                                                                                                                                                                          >  1:1328103/1‑249 (MQ=255)

                 aaaaCCGGACTAAAACCAGACTGTAAAGTGTCAGCACGTTGTGCACACTCAATATCATATCCTTGCGCACGCCCGATCCCACTAGGTTGTGCACACACAAAGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGaa                                                                                                                                                                                                                                   >  1:1328190/1‑249 (MQ=255)

                 aaaaCCGGACTAAAACCAGACTGTAAAGTGTCAGCACGTTGTGCACACTCAATATCATATCCTTGCGCACGCCCGATCCCACTAGGTTGTGCACACACAAAGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGaa                                                                                                                                                                                                                                   >  1:1328078/1‑249 (MQ=255)

                 aaaaCCGGACTAAAACCAGACTGTAAAGTGTCAGCACGTTGTGCACACTCAATATCATATCCTTGCGCACGCCCGATCCCACTAGGTTGTGCACACACAAAGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGaa                                                                                                                                                                                                                                   >  2:1328111/1‑249 (MQ=255)

                  aaaCCGGACTAAAACCAGACTGTAAAGTGTCAGCACGTTGTGCACACTCAATATCATATCCTTGCGCACGCCCGATCCCACTAGGTTGTGCACACACAAAGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAAt                                                                                                                                                                                                                                  <  2:1328052/249‑1 (MQ=255)

                        gACTAAAACCAGACTGTAAAGTGTCAGCACGTTGTGCACACTCAATATCATATCCTTGCGCACGCCCGATCCCACTAGGTTGTGCACACACAAAGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTa                                                                                                                                                                                                                            >  1:1328156/1‑249 (MQ=255)

                          cTAAAACCAGACTGTAAAGTGTCAGCACGTTGTGCACACTCAATATCATATCCTTGCGCACGCCCGATCCCACTAGGTTGTGCACACACAAAGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAg                                                                                                                                                                                                                          <  2:1328055/249‑1 (MQ=255)

                            aaaaCCAGACTGTAAAGTGTCAGCACGTTGTGCACACTCAATATCATATCCTTGCGCACGCCCGATCCCACTAGGTTGTGCACACACAAAGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGaa                                                                                                                                                                                                                        <  2:1328057/249‑1 (MQ=255)

                             aaaCCAGACTGTAAAGTGTCAGCACGTTGTGCACACTCAATATCATATCCTTGCGCACGCCCGATCCCACTAGGTTGTGCACACACAAAGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAAc                                                                                                                                                                                                                       >  1:1328288/1‑249 (MQ=255)

                                    aCTGTAAAGTGTCAGCACGTTGTGCACACTCAATATCATATCCTTGCGCACGCCCGATCCCACTAGGTTGTGCACACACAAAGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCATACCCCACGAATGTTGTAAGAACTGAtata                                                                                                                                                                                                                <  2:1328059/249‑1 (MQ=255)

                                    aCTGTAAAGTGTCAGCACGTTGTGCACACTCAATATCATATCCTTGCGCACGCCCGATCCCACTAGGTTGTGCACACACAAAGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGACTGATGTAAGAACTGAtata                                                                                                                                                                                                                >  2:1328256/1‑249 (MQ=255)

                                    aCTGTAAAGTGTCAGCACGTTGTGCACACTCAATATCATATCCTTGCGCACGCCCGATCCCACTAGGTTGTGCACACACAAAGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGAtata                                                                                                                                                                                                                >  1:1328153/1‑249 (MQ=255)

                                     cTGTAAAGTGTCAGCACGTTGTGCACACTCAATATCATATCCTTGCGCACGCCCGATCCCACTAGGTTGTGCACACACAAAGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATAc                                                                                                                                                                                                               <  2:1328061/249‑1 (MQ=255)

                                      ngTAAAGTGTCAGCACGTTGTGCACACTCAATATCATATCCTTGCGCACGCCCGATCCCACTAGGTTGTGCACACACAAAGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACg                                                                                                                                                                                                              <  1:1328062/248‑1 (MQ=255)

                                        tAAAGTGTCAGCACGTTGTGCACACTCAATATCATATCCTTGCGCACGCCCGATCCCACTAGGTTGTGCACACACAAAGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGAc                                                                                                                                                                                                            >  1:1328196/1‑249 (MQ=255)

                                           aGTGTCAGCACGTTGTGCACACTCAATATCATATCCTTGCGCACGCCCGATCCCACTAGGTTGTGCACACACAAAGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTa                                                                                                                                                                                                         <  1:1328064/249‑1 (MQ=255)

                                              gtCAGCACGTTGTGCACACTCAATATCATATCCTTGCGCACGCCCGATCCCACTAGGTTGTGCACACACAAAGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTAccc                                                                                                                                                                                                      >  1:1328193/1‑249 (MQ=255)

                                                 aGCACGTTGTGCACACTCAATATCATATCCTTGCGCACGCCCGATCCCACTAGGTTGTGCACACACAAAGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGTTATACGACTTACCCCgg                                                                                                                                                                                                   <  2:1328067/249‑1 (MQ=255)

                                                    aCGTTGTGCACACTCAATATCATATCCTTGCGCACGCCCGATCCCACTAGGTTGTGCACACACAAAGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGCCTTAAATCGCTTCTCCGCCTCATCACGTTTGCGTTGCCACAACAGCGCGGGGTGATGAATCCACACCCCACGCATCTTGTAAGACCTGATATACGACTTACCCCGGGCa                                                                                                                                                                                                >  1:1328182/1‑249 (MQ=14)

                                                      gTTGTGCACACTCAATATCATATCCTTGCGCACGCCCGATCCCACTAGGTTGTGCACACACAAAGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAAt                                                                                                                                                                                              <  1:1328069/249‑1 (MQ=255)

                                                       ttGTGCACACTCAATATCATATCCTTGCGCACGCCCGATCCCACTAGGTTGTGCACACACAAAGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATc                                                                                                                                                                                             <  1:1328071/249‑1 (MQ=255)

                                                           gCACACTCAATATCATATCCTTGCGCACGCCCGATCCCACTAGGTTGTGCACACACAAAGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCca                                                                                                                                                                                         <  2:1328072/249‑1 (MQ=255)

                                                            cacaCTCAATATCATATCCTTGCGCACGCCCGATCCCACTAGGTTGTGCACACACAAAGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCcac                                                                                                                                                                                        <  1:1328074/249‑1 (MQ=255)

                                                             acacTCAATATCATATCCTTGCGCACGCCCGATCCCACTAGGTTGTGCACACACAAAGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCcaca                                                                                                                                                                                       <  2:1328075/249‑1 (MQ=255)

                                                              cacTCAATATCATATCCTTGCGCACGCCCGATCCCACTAGGTTGTGCACACACAAAGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACaa                                                                                                                                                                                      >  2:1328124/1‑249 (MQ=255)

                                                                cTCAATATCATATCCTTGCGCACGCCCGATCCCACTAGGTTGTGCACACACAAAGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAAcc                                                                                                                                                                                    >  2:1328147/1‑249 (MQ=255)

                                                                      atcatatcCTTGCGCACGCCCGATCCCACTAGGTTGTGCACACACAAAGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAAc                                                                                                                                                                              <  2:1328078/249‑1 (MQ=255)

                                                                       tcatatcCTTGCGCACGCCCGATCCCACTAGGTTGTGCACACACAAAGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGCTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACg                                                                                                                                                                             >  1:1328347/1‑249 (MQ=255)

                                                                         atatcCTTGCGCACGCCCGATCCCACTAGGTTGTGCACACACAAAGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGaa                                                                                                                                                                           >  1:1328251/1‑249 (MQ=255)

                                                                             ccTTGCGCACGCCCGATCCCACTAGGTTGTGCACACACAAAGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATca                                                                                                                                                                       <  1:1328081/249‑1 (MQ=255)

                                                                               ttGCGCACGCCCGATCCCACTAGGTTGTGCACACACAAAGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATcaac                                                                                                                                                                     >  1:1328316/1‑249 (MQ=255)

                                                                               ntGCGCACGCCCTATCCCACTAGGTTGTGCACACACAAAGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATcaac                                                                                                                                                                     <  2:1328082/248‑1 (MQ=255)

                                                                                  cgcACGCCCGATCCCACTAGGTTGTGCACACACAAAGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTGAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACACCAGCGTCGGGTGCTGCATCCACACCACACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCCATCGCCACAACCGGCCACGAAATAaacaac                                                                                                                                                                  >  2:1328355/1‑249 (MQ=255)

                                                                                   gcACGCCCGATCCCACTAGGTTGTGCACACACAAAGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACt                                                                                                                                                                 >  2:1328212/1‑249 (MQ=255)

                                                                                     aCGCCCGATCCCACTAGGTTGTGCACACACAAAGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCa                                                                                                                                                               >  1:1328178/1‑249 (MQ=255)

                                                                                         ccGATCCCACTAGGTTGTGCACACACAAAGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGCCTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCt                                                                                                                                                           >  2:1328259/1‑249 (MQ=255)

                                                                                          cGATCCCACTAGGTTGTGCACACACAAAGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTc                                                                                                                                                          <  1:1328085/249‑1 (MQ=255)

                                                                                              cccACTAGGTTGTGCACACACAAAGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCg                                                                                                                                                      <  2:1328087/249‑1 (MQ=255)

                                                                                               ccACTAGGTTGTGCACACACAAAGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCgg                                                                                                                                                     >  2:1328220/1‑249 (MQ=255)

                                                                                                cACTAGGTTGTGCACACACAAAGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGt                                                                                                                                                    >  1:1328176/1‑249 (MQ=255)

                                                                                                    aGGTTGTGCACACACAAAGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATc                                                                                                                                                >  2:1328202/1‑249 (MQ=255)

                                                                                                    aGGTTGTGCACACACAAAGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATc                                                                                                                                                <  1:1328089/249‑1 (MQ=255)

                                                                                                    aGGTTGTGCACACACAAAGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCCCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATc                                                                                                                                                >  2:1328230/1‑249 (MQ=255)

                                                                                                       ttGTGCACACACAAAGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCa                                                                                                                                             <  2:1328090/249‑1 (MQ=255)

                                                                                                                 acaAAGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAAc                                                                                                                                   <  1:1328092/249‑1 (MQ=255)

                                                                                                                   aaaGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAACCa                                                                                                                                 >  2:1328181/1‑249 (MQ=255)

                                                                                                                   aaaGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAACCa                                                                                                                                 <  2:1328093/249‑1 (MQ=255)

                                                                                                                     aGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAACCAAg                                                                                                                               <  1:1328095/249‑1 (MQ=255)

                                                                                                                             cagTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAACCAAGCTCAGTTg                                                                                                                       <  1:1328097/249‑1 (MQ=255)

                                                                                                                                  tgCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAACCAAGCTCAGTTGCCGTc                                                                                                                  >  2:1328191/1‑249 (MQ=255)

                                                                                                                                  tgCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAACCAAGCTCAGTTGCCGTc                                                                                                                  <  1:1328099/249‑1 (MQ=255)

                                                                                                                                         cAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAACCAAGCTCAGTTGCCGTCACCGTcc                                                                                                           <  2:1328102/249‑1 (MQ=255)

                                                                                                                                          aaCCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAACCAAGCTCAGTTGCCGTCACCGTCCg                                                                                                          >  1:1328149/1‑249 (MQ=255)

                                                                                                                                              ctcaccctcGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAACCAAGCTCAGTTGCCGTCACCGTCCGATaa                                                                                                      >  2:1328268/1‑249 (MQ=255)

                                                                                                                                                 accctcGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAACCAAGCTCAGTTGCCGTCACCGTCCGATAAAAc                                                                                                   <  1:1328106/249‑1 (MQ=255)

                                                                                                                                                   cctcGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAACCAAGCTCAGTTGCCGTCACCGTCCGATAAAACTc                                                                                                 >  2:1328174/1‑249 (MQ=255)

                                                                                                                                                      cGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAACCAAGCTCAGTTGCCGTCACCGTCCGATAAAACTCCCt                                                                                              <  2:1328108/249‑1 (MQ=255)

                                                                                                                                                         gACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAACCAAGCTCAGTTGCCGTCACCGTCCGATAAAACTCCCTGTc                                                                                           <  1:1328109/249‑1 (MQ=255)

                                                                                                                                                          aCAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAACCAAGCTCAGTTGCCGTCACCGTCCGATAAAACTCCCTGTcc                                                                                          <  1:1328112/249‑1 (MQ=255)

                                                                                                                                                           cAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAACCAAGCTCAGTTGCCGTCACCGTCCGATAAAACTCCCTGTccc                                                                                         >  1:1328161/1‑249 (MQ=255)

                                                                                                                                                               tttCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAACCAAGCTCAGTTGCCGTCACCGTCCGATAAAACTCCCTGTCCCTCAt                                                                                     >  1:1328237/1‑249 (MQ=255)

                                                                                                                                                                 tCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAACCAAGCTCAGTTGCCGTCACCGTCCGATAAAACTCCCTGTCCCTCATCg                                                                                   >  1:1328261/1‑249 (MQ=255)

                                                                                                                                                                 tCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAACCAAGCTCAGTTGCCGTCACCGTCCGATAAAACTCCCTGTCCCTCATCg                                                                                   <  1:1328114/249‑1 (MQ=255)

                                                                                                                                                                 tCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAACCAAGCTCAGTTGCCGTCACCGTCCGATAAAACTCCCTGTCCCTCATCg                                                                                   <  2:1328115/249‑1 (MQ=255)

                                                                                                                                                                        cAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAACCAAGCTCAGTTGCCATCACCGTCCGATAAAACTCCCTGTCCCTCATCGTACGAgg                                                                            <  1:1328118/249‑1 (MQ=255)

                                                                                                                                                                           tAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAACCAAGCTCAGTTGCCGTCACCGTCCGATAAAACTCCCTGTCCCTCATCGTACGAGGAAc                                                                         <  2:1328120/249‑1 (MQ=39)

                                                                                                                                                                              aTCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAACCAAGCTCAGTTGCCGTCACCGTCCGATAAAACTCCCTGTCCCTCATCGTACGAGGAACAGa                                                                      >  1:1328234/1‑249 (MQ=255)

                                                                                                                                                                               tCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAACCAAGCTCAGTTGCCGTCACCGTCCGATAAAACTCCCTGTCCCTCATCGTACGAGGAACAGaa                                                                     >  1:1328207/1‑249 (MQ=255)

                                                                                                                                                                               tCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAACCAAGCTCAGTTGCCGTCACCGTCCGATAAAACTCCCTGTCCCTCATCGTACGAGGAACAGaa                                                                     >  1:1328245/1‑249 (MQ=255)

                                                                                                                                                                                 ccccGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAACCAAGCTCAGTTGCCGTCACCGTCCGATAAAACTCCCTGTCCCTCATCGTACGAGGAACAGAAAt                                                                   <  1:1328123/249‑1 (MQ=39)

                                                                                                                                                                                      gATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAACCAAGCTCAGTTGCCGTCACCGTCCGATAAAACTCCCTGTCCCTCATCGTACGAGGAACAGAAATACGCa                                                              <  1:1328126/249‑1 (MQ=255)

                                                                                                                                                                                       nnnnaTCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAACCAAGCTCAGTTGCCGTCACCGTCCGATAAAACTCCCTGTCCCTCATCGTACGAGGAACAGAAATACGCAt                                                             <  1:1328128/245‑1 (MQ=255)

                                                                                                                                                                                         cTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAACCAAGCTCAGTTGCCGTCACCGTCCGATAAAACTCCCTGTCCCTCATCGTACGAGGAACAGAAATACGCATcc                                                           >  1:1328210/1‑249 (MQ=255)

                                                                                                                                                                                              gACTTAAATCGCTTCTCAGTCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAACCAAGCTCAGTTGCCGTCACCGTCCGATAAAACTCCCTGTCCCTCATCGTACGAGGAACAGAAATACGCATCCCCTgg                                                      <  2:1328130/249‑1 (MQ=255)

                                                                                                                                                                                               aCTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAACCAAGCTCAGTTGCCGTCACCGTCCGATAAAACTCCCTGTCCCTCATCGTACGAGGAACAGAAATACGCATCCCCTGGa                                                     <  2:1328132/249‑1 (MQ=39)

                                                                                                                                                                                               aCTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAACCAAGCTCAGTTGCCGTCACCGTCCGACAAAACTCCCTGTCCCTCATCGTACGAGGACCAGAAATACGCATCCCCTGGa                                                     >  2:1328315/1‑249 (MQ=255)

                                                                                                                                                                                                 ttAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAACCAAGCTCAGTTGCCGTCACCGTCCGATAAAACTCCCTGTCCCTCATCGTACGAGGAACAGAAATACGCATCCCCTGGAac                                                   >  2:1328328/1‑249 (MQ=38)

                                                                                                                                                                                                 ntAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAACCAAGCTCAGTTGCCGTCACCGTCCGATAAAACTCCCTGTCCCTCATCGTACGAGGAACAGAAATACGCATCCCCTGGAac                                                   <  1:1328133/248‑1 (MQ=255)

                                                                                                                                                                                                      tCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAACCAAGCTCAGTTGCCGTCACCGTCCGATAAAACTCCCTGTCCCTCATCGTACGAGGAACAGAAATACGCATCCCCTGGAAcaccac                                              <  2:1328135/249‑1 (MQ=38)

                                                                                                                                                                                                           tctcAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAACCAAGCTCAGTTGCCGTCACCGTCCGATAAAACTCCCTGTCCCTCATCGTACGAGGAACAGAAATACGCATCCCCTGGAACACCACCTTCt                                         >  1:1328267/1‑249 (MQ=39)

                                                                                                                                                                                                             ncAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAACCAAGCTCAGTTGCCGTCACCGTCCGATAAAACTCCCTGTCCCTCATCGTACGAGGAACAGAAATACGCATCCCCTGGAACACCACCTTCTGa                                       <  1:1328136/248‑1 (MQ=38)

                                                                                                                                                                                                                gCCTCATCACGTTTGCGTGGCCACAACAGCGTCGGGGGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAGCCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAACCAAGCTCAGTTGCCGTCACCGTCCGATAAAACTCCCTGTCCCTCATCGTACGAGGAACAGAAATACGCATCCCCTGGAACACCACCTTCTGatta                                    <  2:1328139/249‑1 (MQ=255)

                                                                                                                                                                                                                 ccTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAACCAAGCTCAGTTGCCGTCACCGTCCGATAAAACTCCCTGTCCCTCATCGTACGAGGAACAGAAATACGCATCCCCTGGAACACCACCTTCTGattat                                   >  2:1328325/1‑249 (MQ=37)

                                                                                                                                                                                                                     atcaCGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAACCAAGCTCAGTTGCCGTCACCGTCCGATAAAACTCCCTGTCCCTCATCGTACGAGGAACAGAAATACGCATCCCCTGGAACACCACCTTCTGATTATTccc                               <  2:1328141/249‑1 (MQ=37)

                                                                                                                                                                                                                     atcaCGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAACCAAGCTCAGTTGCCGTCACCGTCCGATAAAACTCCCTGTCCCTCATCGTACGAGGAACAGAAATACGCATCCCCTGGAACACCACCTTCTGATTATTccc                               >  1:1328228/1‑249 (MQ=38)

                                                                                                                                                                                                                       caCGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGGTCCCCGGTTATCGCATCCCAGAAACCAAGCTCAGTTGCCGTCACCGTCCGATAAAACTCCCTGTCCCTCATCGTACGAGGAACAGAAATACGCATCCCCTGGAACACCACCTTCTGATTATTCCCAg                             >  2:1328216/1‑249 (MQ=255)

                                                                                                                                                                                                                       caCGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAACCAAGCTCAGTTGCCGTCACCGTCCGATAAAACTCCCTGTCCCTCATCGTACGAGGAACAGAAATACGCATCCCCTGGAACACCACCTTCTGATTATTCCCAg                             >  2:1328294/1‑249 (MQ=39)

                                                                                                                                                                                                                       caCGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAACCAAGCTCAGTTGCCGTCACCGTCCGATAAAACTCCCTGTCCCTCATCGTACGAGGAACAGAAATACGCATCCCCTGGAACACCACCTTCTGATTATTCCCAg                             <  1:1328142/249‑1 (MQ=38)

                                                                                                                                                                                                                        aCGTTTGCGTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGACAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAACCAAGCTCAGTTGCCGTCACCGTCCGATAAAACTCCCTGTCCCTCATCGTACGAGGAACAGAAATACGCATCCCCTGGAACACCACCTTCTGATTATTCCCAGc                            >  1:1328186/1‑249 (MQ=37)

                                                                                                                                                                                                                                gTTGCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAACCAAGCTCAGTTGCCGTCACCGTCCGATAAAACTCCCTGTCCCTCATCGTACGAGGAACAGAAATACGCATCCCCTGGAACACCACCTTCTGATTATTCCCAGCAAACGTaa                    <  1:1328145/249‑1 (MQ=38)

                                                                                                                                                                                                                                   gCCACAACAGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAACCAAGCTCAGTTGCCGTCACCGTCCGATAAAACTCCCTGTCCCTCATCGTACGAGGAACAGAAATACGCATCCCCTGGAACACCACCTTCTGATTATTCCCAGCAAACGTAAaca                 <  1:1328147/249‑1 (MQ=38)

                                                                                                                                                                                                                                       caacaGCGTCGGGTGCCGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAACCAAGCTCAGTTGCCGTCACCGTCCGATAAAACTCCCTGTCCCTCATCGTACGAGGAACAGAAATACGCATCCCCTGGAACACCACCTTCTGATTATTCCCAGCAAACGTAAACACATc             >  1:1328303/1‑249 (MQ=37)

                                                                                                                                                                                                                                           aGCGTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAACCAAGCTCAGTTGCCGTCACCGTCCGATAAAACTCCCTGTCCCTCATCGTACGAGGAACAGAAATACGCATCCCCTGGAACACCACCTTCTGATTATTCCCAGCAAACGTAAACACATCAGGa         <  2:1328151/249‑1 (MQ=37)

                                                                                                                                                                                                                                              gTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAACCAAGCTCAGTTGCCGTCACCGTCCGATAAAACTCCCTGTCCCTCATCGTACGAGGAACAGAAATACGCATCCCCTGGAACACCACCTTCTGATTATTCCCAGCAAACGTAAACACATCAGGATcc      >  1:1328172/1‑249 (MQ=25)

                                                                                                                                                                                                                                              gTCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAACCAAGCTCAGTTGCCGTCACCGTCCGATAAAACTCCCTGTCCCTCATCGTACGAGGAACAGAAATACGCATCCCCTGGAACACCACCTTCTGATTATTCCCAGCAAACGTAAACACATAAGTATcc      >  2:1328240/1‑249 (MQ=21)

                                                                                                                                                                                                                                               tCGGGTGCTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAACCAAGCTCAGTTGCCGTCACCGTCCGATAAAACTCCCTGTCCCTCATCGTACGAGGAACAGAAATACGCATCCCCTGGAACACCACCTTCTGATTATTCCCAGCAAACGTAAACACATCAGGATCCa     >  1:1328221/1‑249 (MQ=21)

CAAGGGGGTCCAATCCTAAAACCGGACTAAAACCAGACTGTAAAGTGTCAGCACGTTGTGCACACTCAATATCATATCCTTGCGCACGCCCGATCCCACTAGGTTGTGCACACACAAAGCCACAGCAGTGTGCACACCAACCCTCACCCTCGGGACAAGTTTCTAGCGCAGTAGATCCCCCGGATCTATCGACTTAAATCGCTTCTCAGCCTCATCACGTTTGCGTTGCCACAACAGCGTCGGGTGTTGCATCCACACCCCACGAATGTTGTAAGAACTGATATACGACTTACCCCGGGCAATCGCCACAACCGGCAACGAAATCAACAACTCAAGCTCCCCGGTTATCGCATCCCAGAAACCAAGCTCAGTTGCCGTCACCGTCCGATAAAACTCCCTGTCCCTCATCGTACGAGGAACAGAAATACGCATCCCCTGGAACACCACCTTCTGATTATTCCCAGCAAACGTAAACACATCAGGATCCACCG  >  BA000036/3155082‑3155572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`