Predicted mutation
evidence seq id position mutation annotation gene description
RA BA000036 387,460 T→C *66* (TAA→TAG)  Cgl0365 ← Hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BA000036387,4600TC99.6% 994.9 / NA 247*66* (TAA→TAG) Cgl0365Hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (121/125);  total (122/125)
Fisher's exact test for biased strand distribution p-value = 4.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 247 total aligned reads displayed.

ATTATCGACCTTTAACGCCATTGTGATTAGTATTTTGGCGTTGCCTCCTGAGCGACTTGCAAATAGTTCATTTCGGCAGAGTGCTAACGGTTAGGCACTATTTTCCGTTAGTTCTTTTGTAGTCCCGACGCATCTAAGCCGAAACAGCTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCTTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTGGGCCCGGATGTGGGTGTTTTTCATTTTCTTCCACTCTAAAATTAAGTATGGAAAACCAACCGCACCCGGATGCACGACAATGACCCACTAAACACGTATCCTTGAATGCGTGACTGAACATTA  >  BA000036/387214‑387705
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     
  tATCGACCTTTAACGCCATTGTGATTAGTATTTTGGCGTTGCCTCCTGAGCGACTTGCAAATAGTTCATTTCGGCAGAGTGCTAACGGTTAGGCACTATTTTCCGTTAGTTCTTTTGTAGTCCCGACGCATCTAAGCCGAAACAGCTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAgg                                                                                                                                                                                                                                                   <  2:163500/249‑1 (MQ=255)
  tATCGACCTTTAACGCCATTGTGATTAGTATTTTGGCGTTGCCTCCTGAGCGACTTGCAAATAGTTCATTTCGGCAGAGTGCTAACGGTTAGGCACTATTTTCCGTTAGTTCTTTTGTAGTCCCGACGCATCTAAGCCGAAACAGCTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAgg                                                                                                                                                                                                                                                   >  2:163694/1‑249 (MQ=255)
    tCGACCTTTAACGCCATTGTGATTAGTATTTTGGCGTTGCCTCCTGAGCGACTTGCAAATAGTTCATTTCGGCAGAGTGCTAACGGTTAGGCACTATTTTCCGTTAGTTCTTTTGTAGTCCCGACGCATCTAAGCCGAAACAGCTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGtt                                                                                                                                                                                                                                                 >  1:163662/1‑249 (MQ=255)
    tCGACCTTTAACGCCATTGTGATTAGTATTTTGGCGTTGCCTCCTGAGCGACTTGCAAATAGTTCATTTCGGCAGAGTGCTAACGGTTAGGCACTATTTTCCGTTAGTTCTTTTGTAGTCCCGACGCATCTAAGCCGAAACAGCTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGtt                                                                                                                                                                                                                                                 >  2:163682/1‑249 (MQ=255)
      gACCTTTAACGCCATTGTGAGTAGTATTTTGGCGTTGCCGCTTGAGCGACTTGTAAATAGTTCAATTCGGCAGAGTGCTAACAGTTAGGCACTATTTTCCGTTAGTTCTTTTGTAGGCCCGACGCATCTAAGCCGAAAAAGCTAAAAGTCCCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTAc                                                                                                                                                                                                                                               <  2:163502/249‑1 (MQ=255)
           ttAACGCCATTGTGATTAGTATTTTGGCGTTGCCTCCTGAGCGACTTGCAAATAGTTCATTTCGGCAGAGTGCTAACGGTTAGGCACTATTTTCCGTTAGTTCTTTTGTAGTCCCGACGCATCTAAGCCGAAACAGCTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCt                                                                                                                                                                                                                                          <  1:163504/249‑1 (MQ=255)
            tAACGCCATTGTGATTAGTATTTTGGCGTTGCCTCCTGAGCGACTTGCAAATAGTTCATTTCGGCAGAGTGCTAACGGTTAGGCACTATTTTCCGTTAGTTCTTTTGTAGTCCCGACGCATCTAAGCCGAAACAGCTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTg                                                                                                                                                                                                                                         >  1:163720/1‑249 (MQ=255)
               cGCCATTGTGATTAGTATTTTGGCGTTGCCTCCTGAGCGACTTGCAAATAGTTCATTTCGGCAGAGTGCTAACGGTTAGGCACTATTTTCCGTTAGTTCTTTTGTAGTCCCGACGCATCTAAGCCGAAACAGCTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCAt                                                                                                                                                                                                                                      >  2:163551/1‑249 (MQ=255)
                 ccATTGTGATTAGTATTTTGGCGTTGCCTCCTGAGCGACTTGCAAATAGTTCATTTCGGCAGAGTGCTAACGGTTAGGCACTATTTTCCGTTAGTTCTTTTGTAGTCCCGACGCATCTAAGCCGAAACAGCTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGc                                                                                                                                                                                                                                    >  1:163636/1‑249 (MQ=255)
                  cATTGTGATTAGTATTTTGGCGTTGCCTCCTGAGCGACTTGCAAATAGTTCATTTCGGCAGAGTGCTAACGGTTAGGCACTATTTTCCGTTAGTTCTTTTGTAGTCCCGACGCATCTAAGCCGAAACAGCTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCt                                                                                                                                                                                                                                   <  1:163507/249‑1 (MQ=255)
                   aTTGTGATTAGTATTTTGGCGTTGCCTCCTGAGCGACTTGCAAATAGTTCATTTCGGCAGAGTGCTAACGGTTAGGCACTATTTTCCGTTAGTTCTTTTGTAGTCCCGACGCATCTAAGCCGAAACAGCTAACAGTCGCACAGGGCCGTGTACTGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAACTCCTAGGTTACGGCCTGCATGCTg                                                                                                                                                                                                                                  >  1:163677/1‑249 (MQ=255)
                      gtgATTAGTATTTTGGCGTTGCCTCCTGAGCGACTTGCAAATAGTTCATTTCGGCAGAGTGCTAACGGTTAGGCACTATTTTCCGTTAGTTCTTTTGTAGTCCCGACGCATCTAAGCCGAAACAGCTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGAt                                                                                                                                                                                                                               <  2:163510/249‑1 (MQ=255)
                      gtgATTAGTATTTTGGCGTTGCCTCCTGAGCGACTTGCAAATAGTTCATTTCGGCAGAGTGCTAACGGTTAGGCACTATTTTCCGTTAGTTCTTTTGTAGTCCCGACGCATCTAAGCCGAAACAGCTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGAt                                                                                                                                                                                                                               <  1:163509/249‑1 (MQ=255)
                         aTTAGTATTTTGGCGTTGCCTCCTGAGCGACTTGCAAATAGTTCATTTCGGCAGAGTGCTAACGGTTAGGCACTATTTTCCGTTAGTTCTTTTGTAGTCCCGACGCATCTAAGCCGAAACAGCTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGAtcct                                                                                                                                                                                                                            >  2:163626/1‑249 (MQ=255)
                           tAGTATTTTGGCGTTGCCTCCTGAGCGACTTGCAAATAGTTCATTTCGGCAGAGTGCTAACGGTTAGGCACTATTTTCCGTTAGTTCTTTTGTAGTCCCGACGCATCTAAGCCGAAACAGCTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGAtcctcc                                                                                                                                                                                                                          <  2:163514/249‑1 (MQ=255)
                            aGTATTTTGGCGTTGCCTCCTGAGCGACTTGCAAATAGTTCATTTCGGCAGAGTGCTAACGGTTAGGCACTATTTTCCGTTAGTTCTTTTGTAGTCCCGACGCATCTAAGCCGAAACAGCTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCg                                                                                                                                                                                                                         <  1:163515/249‑1 (MQ=255)
                               aTTTTGGCGTTGCCTCCTGAGCGACTTGCAAATAGTTCATTTCGGCAGAGTGCTAACGGTTAGGCACTATTTTCCGTTAGTTCTTTTGTAGTCCCGACGCATCTAAGCCGAAACAGCTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCAt                                                                                                                                                                                                                      <  1:163518/249‑1 (MQ=255)
                                   tGGCGTTGCCTCCTGAGCGACTTGCAAATAGTTCATTTCGGCAGAGTGCTAACGGTTAGGCACTATTTTCCGTTAGTTCTTTTGTAGTCCCGACGCATCTAAGCCGAAACAGCTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAg                                                                                                                                                                                                                  >  1:163683/1‑249 (MQ=255)
                                       gctGCCTCCTGAGCGACTTGCAAATAGTTCATTTCGGCAGAGTGCTAACGGTTAGGCACTATTTTCCGTTAGTTCTCTTGTAGTCCCGACGCATCTAAGCCGAAACAGCTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGTAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAAt                                                                                                                                                                                                              <  2:163521/247‑1 (MQ=255)
                                        ttGCCTCCTGAGCGACTTGCAAATAGTTCATTTCGGCAGAGTGCTAACGGTTAGGCACTATTTTCCGTTAGTTCTTTTGTAGTCCCGACGCATCTAAGCCGAAACAGCTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATc                                                                                                                                                                                                             <  1:163522/249‑1 (MQ=255)
                                         tGCCTCCTGAGCGACTTGCAAATAGTTCATTTCGGCAGAGTGCTAACGGTTAGGCACTATTTTCCGTTAGTTCTTTTGTAGTCCCGACGCATCTAAGCCGAAACAGCTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCt                                                                                                                                                                                                            >  1:163716/1‑249 (MQ=255)
                                               ctGAGCGACTTGCAAATAGTTCATTTCGGCAGAGTGCTAACGGTTAGGCACTATTTTCCGTTAGTTCTTTTGTAGTCCCGACGCATCTAAGCCGAAACAGCTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCca                                                                                                                                                                                                      >  1:163827/1‑249 (MQ=255)
                                                   gCGACTTGCAAATAGTTCATTTCGGCAGAGTGCTAACGGTTAGGCACTATTTTCCGTTAGTTCTTTTGTAGTCCCGACGCATCTAAGCCGAAACAGCTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGc                                                                                                                                                                                                  <  2:163525/249‑1 (MQ=255)
                                                     gACTTGCAAATAGTTCATTTCGGCAGAGTGCTAACGGTTAGGCACTATTTTCCGTTAGTTCTTTTGTAGTCCCGACGCATCTAAGCCGAAACAGCTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCAt                                                                                                                                                                                                >  1:163653/1‑249 (MQ=255)
                                                           cAAATAGTTCATTTCGGCAGAGTGCTAACGGTTAGGCACTATTTTCCGTTAGTTCTTTTGTAGTCCCGACGCATCTAAGCCGAAACAGCTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCtt                                                                                                                                                                                          >  2:163666/1‑249 (MQ=255)
                                                            aaaTAGTTCATTTCGGCAGAGTGCTAACGGTTAGGCACTATTTTCCGTTAGTTCTTTTGTAGTCCCGACGCATCTAAGCCGAAACAGCTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTg                                                                                                                                                                                         <  1:163527/249‑1 (MQ=255)
                                                               tAGTTCATTTCGGCAGAGTGCTAACGGTTAGGCACTATTTTCCGTTAGTTCTTTTGTAGTCCCGACGCATCTAAGCCGAAACAGCTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTc                                                                                                                                                                                      >  1:163565/1‑249 (MQ=255)
                                                                aGTTCATTTCGGCAGAGTGCTAACGGTTAGGCACTATTTTCCGTTAGTTCTTTTGTAGTCCCGACGCATCTAAGCCGAAACAGCTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGTTCCTCCGCCTTCAGTAATCTTTCCCACAGCATCGGCTTTTTCt                                                                                                                                                                                     >  2:163602/1‑249 (MQ=255)
                                                                   tCATTTCGGCAGAGTGCTAACGGTTAGGCACTATTTTCCGTTAGTTCTTTTGTAGTCCCGACGCATCTAAGCCGAAACAGCTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTaaa                                                                                                                                                                                  <  1:163530/249‑1 (MQ=255)
                                                                         cGGCAGAGTGCTAACGGTTAGGCACTATTTTCCGTTAGTTCTTTTGTAGTCCCGACGCATCTAAGCCGAAACAGCTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCa                                                                                                                                                                            <  1:163532/249‑1 (MQ=255)
                                                                             agagTGCTAACGGTTAGGCACTATTTTCCGTTAGTTCTTTTGTAGTCCCGACGCATCTAAGCCGAAACAGCTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGt                                                                                                                                                                        >  1:163643/1‑249 (MQ=255)
                                                                                    naaCGGTTAGGCACTATTTTCCGTTAGTTCTTTTGTAGTCCCGACGCATCTAAGCCGAAACAGCTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGa                                                                                                                                                                 <  2:163536/248‑1 (MQ=255)
                                                                                      aCGGTTAGGCACTATTTTCCGTTAGTTCTTTTGTAGTCCCGACGCATCTAAGCCGAAACAGCTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGAcc                                                                                                                                                               <  1:163537/249‑1 (MQ=255)
                                                                                        ggTTAGGCACTATTTTCCGTTAGTTCTTTTGTAGTCCCGACGCATCTAAGCCGAAACAGCTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGt                                                                                                                                                             >  1:163742/1‑249 (MQ=255)
                                                                                          ttAGGCACTATTTTCCGTTAGTTCTTTTGTAGTCCCGACGCATCTAAGCCGAAACAGCTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTAc                                                                                                                                                           <  1:163539/249‑1 (MQ=255)
                                                                                               cACTATTTTCCGTTAGTTCTTTTGTAGTCCCGACGCATCTAAGCCGAAACAGCTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATaaa                                                                                                                                                      <  2:163543/249‑1 (MQ=255)
                                                                                                 cTATTTTCCGTTAGTTCTTTTGTAGTCCCGACGCATCTAAGCCGAAACAGCTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAac                                                                                                                                                    >  1:163625/1‑249 (MQ=255)
                                                                                                 cTATTTTCCGTTAGTTCTTTTGTAGTCCCGACGCATCTAAGCCGAAACAGCTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAac                                                                                                                                                    >  2:163675/1‑249 (MQ=255)
                                                                                                  tATTTTCCGTTAGTTCTTTTGTAGTCCCGACGCATCTAAGCCGAAACAGCTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAaca                                                                                                                                                   >  2:163751/1‑249 (MQ=255)
                                                                                                    ttttCCGTTAGTTCTTTTGTAGTCCCGACGCATCTAAGCCGAAACAGCTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACAcc                                                                                                                                                 <  1:163545/249‑1 (MQ=255)
                                                                                                          gTTAGTTCTTTTGTAGTCCCGACGCATCTAAGCCGAAACAGCTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCAtt                                                                                                                                           <  2:163548/249‑1 (MQ=255)
                                                                                                            tAGTTCTTTTGTAGTCCCGACGCATCTAAGCCAAAACAGCTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTc                                                                                                                                         <  1:163549/249‑1 (MQ=255)
                                                                                                              gTTCTTTTGTAGTCCCGACGCATCTAAGCCGAAACAGCTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCtg                                                                                                                                       >  2:163659/1‑249 (MQ=255)
                                                                                                                    ttGTAGTCCCGACGCATCTAAGCCGAAACAGCTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCa                                                                                                                                 >  1:163667/1‑249 (MQ=255)
                                                                                                                    ttGTAGTCCCGACGCATCTAAGCCGAAACAGCTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCa                                                                                                                                 >  2:163702/1‑249 (MQ=255)
                                                                                                                      gTAGTCCCGACGCATCTAAGCCGAAACAGCTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCAtt                                                                                                                               >  1:163712/1‑249 (MQ=255)
                                                                                                                       tAGTCCCGACGCATCTAAGCCGAAACAGCTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCAttt                                                                                                                              <  1:163553/249‑1 (MQ=255)
                                                                                                                        aGTCCCGACGCATCTAAGCCGAAACAGCTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTg                                                                                                                             <  2:163556/249‑1 (MQ=255)
                                                                                                                          tCCCGACGCATCTAAGCCGAAACAGCTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTggg                                                                                                                           <  2:163558/249‑1 (MQ=255)
                                                                                                                          tCCCGACGCATCTAAGCCAAAACAGCTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTggg                                                                                                                           <  1:163559/249‑1 (MQ=255)
                                                                                                                                          ccGAAACAGCTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTGGGCCCGGATGTGGGTGtt                                                                                                           <  2:163563/249‑1 (MQ=255)
                                                                                                                                          ccGAAACAGCTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTGGGCCCGGATGTGGGTGtt                                                                                                           <  2:163565/249‑1 (MQ=255)
                                                                                                                                            gAAACAGCTAAAAGTCGCACAGGGCCGTGT‑ATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTGGGCCCGGATGTGGGTGttttt                                                                                                        >  1:163693/1‑249 (MQ=255)
                                                                                                                                                   cTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTCAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCTTCAACGAAATCCTAGTTTACGGCCTGCATGCTGAATCCTCCGCTTACAATTATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTATGCCCATTTAGGCCCGGATGTGGGCGTTTTTCAtttt                                                                                                  >  2:163745/1‑249 (MQ=255)
                                                                                                                                                         gTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTGGGCCCGGATGTGGGTGTTTTTCATTTTCTTCCa                                                                                            <  1:163568/249‑1 (MQ=255)
                                                                                                                                                           cGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTGGGCCCGGATGTGGGTGTTTTTCATTTTCTTCCAct                                                                                          <  2:163569/249‑1 (MQ=255)
                                                                                                                                                            gCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTGGGCCCGGATGTGGGTGTTTTTCATTTTCTTCCActc                                                                                         >  2:163722/1‑249 (MQ=255)
                                                                                                                                                            gCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTGGGCCCGGATGTGGGTGTTTTTCATTTTCTTCCActc                                                                                         >  1:163700/1‑249 (MQ=255)
                                                                                                                                                               caGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTGGGCCCGGATGTGGGTGTTTTTCATTTTCTTCCACTCTaa                                                                                      <  2:163572/249‑1 (MQ=255)
                                                                                                                                                                aGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTGGGCCCGGATGTGGGTGTTTTTCATTTTCTTCCACTCTaaa                                                                                     <  1:163574/249‑1 (MQ=255)
                                                                                                                                                                      gtgtAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTGGGCCCGGATGTGGGTGTTTTTCATTTTCTTCCACTCTAAAATTAAg                                                                               >  1:163707/1‑249 (MQ=255)
                                                                                                                                                                       tgtAATGCACGTAAGAGATAGTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTGGGCCCGGATGTGGGTGTTTTTCATTTTCTTCCACTCTAAAATTAAGt                                                                              <  2:163577/249‑1 (MQ=255)
                                                                                                                                                                           aTGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTGGGCCCGGATGTGGGTGTTTTTCATTTTCTTCCACTCTAAAATTAAGTATgg                                                                          <  1:163579/249‑1 (MQ=255)
                                                                                                                                                                             gCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTGGGCCCGGATGTGGGTGTTTTTCATTTTCTTCCACTCTAAAATTAAGTATGGaa                                                                        <  1:163581/249‑1 (MQ=255)
                                                                                                                                                                               ccGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTGGGCCCGGATGTGGGTGTTTTTCATTTTCTTCCACTCTAAAATTAAGTATGGaaaa                                                                      <  2:163584/249‑1 (MQ=255)
                                                                                                                                                                                  tAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTGGGCCCGGATGTGGGTGTTTTTCATTTTCTTCCACTCTAAAATTAAGTATGGAAaacca                                                                   >  2:163801/1‑249 (MQ=255)
                                                                                                                                                                                    agagATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTGGGCCCGGATGTGGGTGTTTTTCATTTTCTTCCACTCTAAAATTAAGTATGGAAaaccaac                                                                 <  1:163587/249‑1 (MQ=255)
                                                                                                                                                                                         tatTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTGGGCCCGGATGTGGGTGTTTTTCATTTTCTTCCACTCTAAAATTAAGTATGGAAAACCAACCGCAc                                                            <  2:163588/249‑1 (MQ=255)
                                                                                                                                                                                          atTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTGGGCCCGGATGTGGGTGTTTTTCATTTTCTTCCACTCTAAAATTAAGTATGGAAAACCAACCGCAcc                                                           >  1:163688/1‑249 (MQ=255)
                                                                                                                                                                                          atTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTGGGCCCGGATGTGGGTGTTTTTCATTTTCTTCCACTCTAAAATTAAGTATGGAAAACCAACCGCAcc                                                           >  1:163730/1‑249 (MQ=255)
                                                                                                                                                                                             tAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTGGGCCCGGATGTGGGTGTTTTTCATTTTCTTCCACTCTAAAATTAAGTATGGAAAACCAACCGCACCCgg                                                        <  1:163591/249‑1 (MQ=255)
                                                                                                                                                                                              aaGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTGGGCCCGGATGTGGGTGTTTTTCATTTTCTTCCACTCTAAAATTAAGTATGGAAAACCAACCGCACCCGGa                                                       <  2:163592/249‑1 (MQ=255)
                                                                                                                                                                                                  aaTCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTGGGCCCGGATGTGGGTGTTTTTCATTTTCTTCCACTCTAAAATTAAGTATGGAAAACCAACCGCACCCGGATGCa                                                   <  2:163595/249‑1 (MQ=255)
                                                                                                                                                                                                      naaaCTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTGGGCCCGGATGTGGGTGTTTTTCATTTTCTTCCACTCTAAAATTAAGTATGGAAAACCAACCGCACCCGGATGCACGAc                                               <  1:163597/248‑1 (MQ=255)
                                                                                                                                                                                                        aaCTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTGGGCCCGGATGTGGGTGTTTTTCATTTTCTTCCACTCTAAAATTAAGTATGGAAAACCAACCGCACCCGGATGCACGACaa                                             <  2:163599/249‑1 (MQ=255)
                                                                                                                                                                                                            aTGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTGGGCCCGGATGTGGGTGTTTTTCATTTTCTTCCACTCTAAAATTAAGTATGGAAAACCAACCGCACCCGGATGCACGACAATGAc                                         >  2:163732/1‑249 (MQ=255)
                                                                                                                                                                                                               ccAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTGGGCCCGGATGTGGGTGTTTTTCATTTTCTTCCACTCTAAAATTAAGTATGGAAAACCAACCGCACCCGGATGCACGACAATGACCCa                                      <  1:163601/249‑1 (MQ=255)
                                                                                                                                                                                                                cAATTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTGGGCCCGGATGTGGGTGTTTTTCATTTTCTTCCACTCTAAAATTAAGTATGGAAAACCAACCCCACCCGGAGGCACGACAATGACCCAc                                     >  2:163765/1‑249 (MQ=255)
                                                                                                                                                                                                                  aTTCATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTGGGCCCGGATGTGGGTGTTTTTCATTTTCTTCCACTCTAAAATTAAGTATGGAAAACCAACCGCACCCGGATGCACGACAATGACCCACTa                                   >  2:163825/1‑249 (MQ=255)
                                                                                                                                                                                                                     cATGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTGGGCCCGGATGTGGGTGTTTTTCATTTTCTTCCACTCTAAAATTAAGTATGGAAAACCAACCGCACCCGGATGCACGACAATGACCCACTAAac                                <  1:163604/249‑1 (MQ=255)
                                                                                                                                                                                                                       tGAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTGGGCCCGGATGTGGGTGTTTTTCATTTTCTTCCACTCTAAAATTAAGTATGGAAAACCAACCGCACCCGGATGCACGACAATGACCCACTAAacac                              <  2:163607/249‑1 (MQ=255)
                                                                                                                                                                                                                        gAACAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTGGGCCCGGATGTGGGTGTTTTTCATTTTCTTCCACTCTAAAATTAAGTATGGAAAACCAACCGCACCCGGATGCACGACAATGACCCACTAAACACg                             <  1:163608/249‑1 (MQ=255)
                                                                                                                                                                                                                        caaCAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTGGGCCCGGATGTGGGTGTTTTTCATTTTCTTCCACTCTAAAATTAAGTATGGAAAACCAACCGCACCCGGATGCACGACAATGACCCACTAAACACg                             >  1:163762/2‑249 (MQ=255)
                                                                                                                                                                                                                           cAGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTGGGCCCGGATGTGGGTGTTTTTCATTTTCTTCCACTCTAAAATTAAGTATGGAAAACCAACCGCACCCGGATGCACGACAATGACCCACTAAACACGTAt                          <  2:163610/249‑1 (MQ=255)
                                                                                                                                                                                                                            aGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTGGGCCCGGATGTGGGTGTTTTTCATTTTCTTCCACTCTAAAATTAAGTATGGAAAACCAACCGCACCCGGATGCACGACAATGACCCACTAAACACGTATc                         >  2:163835/1‑249 (MQ=255)
                                                                                                                                                                                                                            aGATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTGGGCCCGGATGTGGGTGTTTTTCATTTTCTTCCACTCTAAAATTAAGTATGGAAAACCAACCGCACCCGGATGCACGACAATGACCCACTAAACACGTATc                         >  1:163837/1‑249 (MQ=255)
                                                                                                                                                                                                                             gATCATCTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTGGGCCCGGATGTGGGTGTTTTTCATTTTCTTCCACTCTAAAATTAAGTATGGAAAACCAACCGCACCCGGATGCACGACAATGACCCACTAAACACGTATcc                        <  1:163611/249‑1 (MQ=255)
                                                                                                                                                                                                                                catcTTCTTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTGGGCCCGGATGTGGGTGTTTTTCATTTTCTTCCACTCTAAAATTAAGTATGGAAAACCAACCGCACCCGGATGCACGACAATGACCCACTAAACACGTATCCTTg                     <  1:163614/249‑1 (MQ=255)
                                                                                                                                                                                                                                  tcttctTCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTGGGCCCGGCTGTGGGTGTTTTTCATTTTCTTCTACTCTAAAATTAAGTATGGAAAACCAACCGCACCCGGATGCACGACAATGACCCACTAAACACGTATCCTTGaa                   >  2:163699/1‑249 (MQ=255)
                                                                                                                                                                                                                                   cttcttCATCAACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTGGGCCCGGATGTGGGTGTTTTTCATTTTCTTCCACTCTAAAATTAAGTATGGAAAACCAACCGCACCCGGATGCACGACAATGACCCACTAAACACGTATCCTTGAAt                  >  1:163799/1‑249 (MQ=255)
                                                                                                                                                                                                                                        tcatcaACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTGGGCCCGGATGTGGGTGTTTTTCATTTTCTTCCACTCTAAAATTAAGTATGGAAAACCAACCGCACCCGGATGCACGACAATGACCCACTAAACACGTATCCTTGAATGGGtt             >  1:163890/1‑248 (MQ=255)
                                                                                                                                                                                                                                        tcatcaACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTGGGCCCGGATGTGGGTGTTTTTCATTTTCTTCCACTCTAAAATTAAGTATGGAAAACCAACCGCACCCGGATGCACGACAATGACCCACTAAACACGTATCCTTGAATGCGTg             <  2:163617/249‑1 (MQ=255)
                                                                                                                                                                                                                                           tcaACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTGGGCCCGGATGTGGGTGTTTTTCATTTTCTTCCACTCTAAAATTAAGTATGGAAAACCAACCGCACCCGGATGCACGACAATGACCCACTAAACACGTATCCTTGAATGCGTGACt          <  1:163618/249‑1 (MQ=255)
                                                                                                                                                                                                                                            caACGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTGGGCCCGGATGTGGGTGTTTTTCATTTTCTTCCACTCTAAAATTAAGTATGGAAAACCAACCGCACCCGGATGCACGACAATGACCCACTAAACACGTATCCTTGAATGCGTGACTg         <  2:163619/249‑1 (MQ=255)
                                                                                                                                                                                                                                             aaCGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTGGGCCCGGATGTGGGTGTTTTTCATTTTCTTCCACTCTAAAATTAAGTATGGAAAACCAACCGCACCCGGATGCACGACAATGACCCACTAAACACGTATCCTTGAATGCGTGACTGa        >  1:163727/1‑249 (MQ=255)
                                                                                                                                                                                                                                              aCGAAATCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTGGGCCCGGATGTGGGTGTTTTTCATTTTCTTCCACTCTAAAATTAAGTATTGAAAACCAACCGCACCCGGATGCACGACAATGACCCACTAAACACGTAACCTTGAATGCGTGACTGaa       >  2:163761/1‑249 (MQ=255)
                                                                                                                                                                                                                                                 aaaTCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTGGGCCCGGATGTGGGTGTTTTTCATTTTCTTCCACTCTAAAATTAAGTATGGAAAACCAACCGCACCCGGATGCACGACAATGACCCACTAAACACGTATCCTTGAATGCGTGCCTGAACAt    >  2:163806/1‑249 (MQ=255)
                                                                                                                                                                                                                                                 aaaTCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTGGGCCCGGATGTGGGTGTTTTTCATTTTCTTCCACTCTAAAATTAAGTATGGAAAACCAACCGCACCCGGATGCACGACAATGACCCACTAAACACGTATCCTTGAATGCGTGACTGAACAt    <  1:163621/249‑1 (MQ=255)
                                                                                                                                                                                                                                                  aaTCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTGGGCCCGGATGTGGGTGTTTTTCATTTTCTTCCACTCTAAAATTAAGTATGGAAAACCAACCGCACCCGGATGCACGACAATGACCCACTAAACACGTATCCTTGAATGCGTGACTGAACAtt   <  2:163623/249‑1 (MQ=255)
                                                                                                                                                                                                                                                  aaTCCTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTGGGCCCGGATGTGGGTGTTTTTCATTTTCTTCCACTCTAAAATTAAGTATGGAAAACCAACCGCACCCGGATGCACGACAATGACCCACTAAACACGTATCCTTGAATGCGTGACTGAACAtt   >  1:163791/1‑249 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     
ATTATCGACCTTTAACGCCATTGTGATTAGTATTTTGGCGTTGCCTCCTGAGCGACTTGCAAATAGTTCATTTCGGCAGAGTGCTAACGGTTAGGCACTATTTTCCGTTAGTTCTTTTGTAGTCCCGACGCATCTAAGCCGAAACAGCTAAAAGTCGCACAGGGCCGTGTAATGCCCGTAAGAGATATTTAAGGAATCTAAACTATGCCAATTCATGAACAGATCATCTTCTTCATCAACGAAATCTTAGGTTACGGCCTGCATGCTGGATCCTCCGCATTCAGTAATCTTTCCCACAGCATCGGCTTGTTCTAAAACAGCACGGTGCTTAGACCGTACATAAAACACCTGCATTTCTGTGCCCATTTGGGCCCGGATGTGGGTGTTTTTCATTTTCTTCCACTCTAAAATTAAGTATGGAAAACCAACCGCACCCGGATGCACGACAATGACCCACTAAACACGTATCCTTGAATGCGTGACTGAACATTA  >  BA000036/387214‑387705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑