BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BA000036	1,751,737	G→A	V81I (GTC→ATC)	Cgl1651 →	Hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BA000036	1,751,737	0	G	A	99.5%	850.3 / NA	204	V81I (GTC→ATC)	Cgl1651	Hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (99/103); total (101/103)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 207 total aligned reads displayed.

CAGGAAATGAAGCTTTTCTCCCGCACCTCACTGGTTGCACTTGGTACCGCTGCAGCTATCACTCTCTCCGGCGTAACCGCTCCTGCATTCGCTGATGAGGACTCCAACGCAGCTGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCGTCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCAAAGATCATTGCCAAGAACGAAGCAGGCGTTGAAGCAGAAGTTTACGTTAACTTTGATTTCAACGAAGAGCCTTCCAGCGAAGAGCCTTCCAGCGGTTCTTCCGACACTGACAACATCGAAAACTGGATCAAGATC  >  BA000036/1751491‑1751982

cAGGAAATGAAGCTTTTCTCCCGCACCTCACTGGTTGCACTTGGTACCGCTGCAGCTATCACTCTCTCCGGCGTAACCGCTCCTGCATTCGCTGATGAGGACTCCAACGCAGCTGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGgtcatc                                                                                                                                                                                                                                                     <  1:678034/249‑1 (MQ=255)

cAGGAAATGAAGCTTTTCTCCCGCACCTCACTGGTTGCACTTGGTACCGCTGCAGCTATCACTCTCTCCGGCGTAACCGCTCCTGCATTCGCTGATGAGGACTCCAACGCAGCTGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGgtcatc                                                                                                                                                                                                                                                     >  2:678221/1‑249 (MQ=255)

   gAAATGAAGCTTTTCTCCCGCACCTCACTGGTTGCACTTGGTACCGCTGCAGCTATCACTCTCTCCGGCGTAACCGCTCCTGCATTCGCTGATGAGGACTCCAACGCAGCTGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCa                                                                                                                                                                                                                                                  <  2:678035/249‑1 (MQ=255)

    aaaTGAAGCTTTTCTCCCGCACCTCACTGGTTGCACTTGGTACCGCTGCAGCTATCACTCTCTCCGGCGTAACCGCTCCTGCATTCGCTGATGAGGACTCCAACGCAGCTGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAg                                                                                                                                                                                                                                                 <  1:678038/249‑1 (MQ=255)

         aaGCTTTTCTCCCGCACCTCACTGGTTGCACTTGGTACCGCTGCAGCTATCACTCTCTCCGGCGTAACCGCTCCTGCATTCGCTGATGAGGACTCCAACGCAGCTGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGaa                                                                                                                                                                                                                                            <  2:678039/249‑1 (MQ=255)

         aaGCTTTTCTCCCGCACCTCACTGGTTGCACTTGGTACCGCTGCAGCTATCACTCTCTCCGGCGTAACCGCTCCTGCATTCGCTGATGAGGACTCCAACGCAGCTGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACAATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAAGCGan                                                                                                                                                                                                                                            >  2:678273/1‑248 (MQ=255)

           gCTTTTCTCCCGCACCTCACTGGTTGCACTTGGTACCGCTGCAGCTATCACTCTCTCCGGCGTAACCGCTCCTGCATTCGCTGATGAGGACTCCAACGCAGCTGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGc                                                                                                                                                                                                                                          >  2:678206/1‑249 (MQ=255)

            cTTTTCTCCCGCACCTCACTGGTTGCACTTGGTACCGCTGCAGCTATCACTCTCTCCGGCGTAACCGCTCCTGCATTCGCTGATGAGGACTCCAACGCAGCTGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCa                                                                                                                                                                                                                                         <  1:678042/249‑1 (MQ=255)

             ntttCTCCCGCACCTCACTGGTTGCACTTGGTACCGCTGCAGCTATCACTCTCTCCGGCGTAACCGCTCCTGCATTCGCTGATGAGGACTCCAACGCAGCTGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAg                                                                                                                                                                                                                                        <  2:678043/248‑1 (MQ=255)

                 nncccGCACCTCACTGGTTGCACTTGGTACCGCTGCAGCTATCACTCTCTCCGGCGTAACCGCTCCTGCATTCGCTGATGAGGACTCCAACGCAGCTGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATc                                                                                                                                                                                                                                    <  2:678046/247‑1 (MQ=255)

                 ctcCCGCACCTCACTGGTTGCACTTGGTACCGCTGCAGCTATCACTCTCTCCGGCGTAACCGCTCCTGCATTCGCTGATGAGGACTCCAACGCAGCTGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATc                                                                                                                                                                                                                                    >  1:678238/1‑249 (MQ=255)

                  ncccGCACCTCACTGGTTGCACTTGGTACCGCTGCAGCTATCACTCTCTCCGGCGTAACCGCTCCTGCATTCGCTGATGAGGACTCCAACGCAGCTGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATcc                                                                                                                                                                                                                                   <  1:678047/248‑1 (MQ=255)

                       cACCTCACTGGTTGCACTTGGTACCGCTGCAGCTATCACTCTCTCCGGCGTAACCGCTCCTGCATTCGCTGATGAGGACTCCAACGCAGCTGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCAc                                                                                                                                                                                                                              >  1:678234/1‑249 (MQ=255)

                            cACTGGTTGCACTTGGTACCGCTGCAGCTATCACTCTCTCCGGCGTAACCGCTCCTGCATTCGCTGATGAGGACTCCAACGCAGCTGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAg                                                                                                                                                                                                                         <  2:678050/249‑1 (MQ=255)

                             aCTGGTTGCACTTGGTACCGCTGCAGCTATCACTCTCTCCGGCGTAACCGCTCCTGCATTCGCTGATGAGGACTCCAACGCAGCTGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGa                                                                                                                                                                                                                        <  2:678052/249‑1 (MQ=255)

                              cTGGTTGCACTTGGTACCGCTGCAGCTATCACTCTCTCCGGCGTAACCGCTCCTGCATTCGCTGATGAGGACTCCAACGCAGCTGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGAc                                                                                                                                                                                                                       >  2:678179/1‑249 (MQ=255)

                              cTGGTTGCACTTGGTACCGCTGCAGCTATCACTCTCTCCGGCGTAACCGCTCCTGCATTCGCTGATGAGGACTCCAACGCAGCTGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGAc                                                                                                                                                                                                                       >  2:678157/1‑249 (MQ=255)

                                ggTTGCACTTGGTACCGCTGCAGCTATCACTCTCTCCGGCGTAACCGCTCCTGCATTCGCTGATGAGGACTCCAACGCAGCTGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACaa                                                                                                                                                                                                                     <  1:678053/249‑1 (MQ=255)

                                   tGCACTTGGTACCGCTGCAGCTATCACTCTCTCCGGCGTAACCGCTCCTGCATTCGCTGATGAGGACTCCAACGCAGCTGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAAcct                                                                                                                                                                                                                  >  1:678301/1‑249 (MQ=255)

                                     cACTTGGTACCGCTGCAGCTATCACTCTCTCCGGCGTAACCGCTCCTGCATTCGCTGATGAGGACTCCAACGCAGCTGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAAccttc                                                                                                                                                                                                                >  1:678223/1‑249 (MQ=255)

                                        ntGGTACCGCTGCAGCTATCACTCTCTCCGGCGTAACCGCTCCTGCATTCGCTGATGAGGACTCCAACGCAGCTGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAAccttcctt                                                                                                                                                                                                             <  1:678057/248‑1 (MQ=255)

                                        ntGGTACCGCTGCAGCTATCACTCTCTCCGGCGTAACCGCGCCTGCATTCGCTGATGAGGACTCCAACGCAGCTGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAAccttcctt                                                                                                                                                                                                             <  2:678056/248‑1 (MQ=255)

                                         tGGTACCGCTGCAGCTATCACTCTCTCCGGCGTAACCGCTCCTGCATTCGCTGATGAGGACTCCAACGCAGCTGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACcttccttc                                                                                                                                                                                                            >  1:678101/1‑249 (MQ=255)

                                         tGGTACCGCTGCAGCTATCACTCTCTCCGGCGTAACCGCTCCTGCATTCGCTGATGAGGACTCCAACGCAGCTGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACcttccttc                                                                                                                                                                                                            >  1:678152/1‑249 (MQ=255)

                                            tACCGCTGCAGCTATCACTCTCTCCGGCGTAACCGCTCCTGCATTCGCTGATGAGGACTCCAACGCAGCTGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTgg                                                                                                                                                                                                         <  1:678059/249‑1 (MQ=255)

                                             aCCGCTGCAGCTATCACTCTCTCCGGCGTAACCGCTCCTGCATTCGCTGATGAGGACTCCAACGCAGCTGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGt                                                                                                                                                                                                        >  2:678119/1‑249 (MQ=255)

                                                gCTGCAGCTATCACTCTCTCCGGCGTAACCGCTCCTGCATTCGCTGATGACGACTCCAACGCAGCTGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGtctc                                                                                                                                                                                                     >  1:678202/1‑249 (MQ=255)

                                                   gCAGCTATCACTCTCTCCGGCGTAACCGCTCCTGCATTCGCTGATGAGGACTCCAACGCAGCTGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCtt                                                                                                                                                                                                  <  2:678061/249‑1 (MQ=255)

                                                         aTCACTCTCTCCGGCGTAACCGCTCCTGCATTCGCTGATGAGGACTCCAACGCAGCTGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGac                                                                                                                                                                                            <  1:678063/249‑1 (MQ=255)

                                                          tCACTCTCTCCGGCGTAACCGCTCCTGCATTCGCTGATGAGGACTCCAACGCAGCTGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGaca                                                                                                                                                                                           <  1:678065/249‑1 (MQ=255)

                                                                 nnccGGCGTAACCGCTCCTGCATTCGCTGATGAGGACTCCAACGCAGCTGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCgg                                                                                                                                                                                    <  1:678067/247‑1 (MQ=255)

                                                                     ggCGTAACCGCTCCTGCATTCGCTGATGAGGACTCCAACGCAGCTGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAg                                                                                                                                                                                <  1:678069/249‑1 (MQ=255)

                                                                            ccGCTCCTGCATTCGCTGATGAGGACTCCAACGCAGCTGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCg                                                                                                                                                                         >  1:678182/1‑249 (MQ=255)

                                                                             cGCTCCTGCATTCGCTGATGAGGACTCCAACGCAGCTGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCgg                                                                                                                                                                        <  2:678072/249‑1 (MQ=255)

                                                                                   tGCATTCGCTGATGAGGACTCCAACGCAGCTGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCAGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGAAAACCTTCCTACTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTcc                                                                                                                                                                  >  2:678189/1‑249 (MQ=255)

                                                                                    gCATTCGCTGATGAGGACTCCAACGCAGCTGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCa                                                                                                                                                                 >  2:678174/1‑249 (MQ=255)

                                                                                      aTTCGCTGATGAGGACTCCAACGCAGCTGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCaaa                                                                                                                                                               >  2:678083/1‑249 (MQ=255)

                                                                                          gCTGATGAGGACTCCAACGCAGCTGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAaagaag                                                                                                                                                           <  1:678075/249‑1 (MQ=255)

                                                                                                gaGGACTCCAACGCAGCTGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTc                                                                                                                                                     >  1:678168/1‑249 (MQ=255)

                                                                                                gaGGACTCCAACGCAGCTGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTc                                                                                                                                                     >  2:678216/1‑249 (MQ=255)

                                                                                                gaGGACTCCAACGCAGCTGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTc                                                                                                                                                     >  1:678220/1‑249 (MQ=255)

                                                                                                  ggACTCCAACGCAGCTGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCAc                                                                                                                                                   >  2:678169/1‑249 (MQ=255)

                                                                                                    aCTCCAACGCAGCTGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTg                                                                                                                                                 <  1:678077/249‑1 (MQ=255)

                                                                                                    aCTCCAACGCAGCTGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTg                                                                                                                                                 >  2:678245/1‑249 (MQ=255)

                                                                                                     cTCCAACGCAGCTGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCCACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTgg                                                                                                                                                <  2:678078/249‑1 (MQ=255)

                                                                                                      tCCAACGCAGCTGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGt                                                                                                                                               >  1:678187/1‑249 (MQ=255)

                                                                                                      tCCAACGCAGCTGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGt                                                                                                                                               >  2:678141/1‑249 (MQ=255)

                                                                                                          aCGCAGCTGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCg                                                                                                                                           <  1:678081/249‑1 (MQ=255)

                                                                                                             cAGCTGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCaa                                                                                                                                        >  1:678165/1‑249 (MQ=255)

                                                                                                               gCTGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCAAAg                                                                                                                                      <  1:678083/249‑1 (MQ=255)

                                                                                                                 tGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCaannnn                                                                                                                                    >  1:678269/1‑245 (MQ=255)

                                                                                                                 ngTAAGTGCTCTTAAGCCCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCAAAGAt                                                                                                                                    <  2:678086/248‑1 (MQ=255)

                                                                                                                 ngTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCAAAGAt                                                                                                                                    <  2:678088/248‑1 (MQ=255)

                                                                                                                  gTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCAAAGATc                                                                                                                                   <  2:678090/249‑1 (MQ=255)

                                                                                                                       tGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCAAAGATCATTGc                                                                                                                              >  2:678160/1‑249 (MQ=255)

                                                                                                                           nntAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCAAAGATCATTGCCAAg                                                                                                                          <  2:678093/247‑1 (MQ=255)

                                                                                                                             tAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAAGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACAGGTACTCCAAAGAAGGAATTCACTGGTTCCGCAAAGATCATTGCCACGaa                                                                                                                        >  1:678217/1‑249 (MQ=255)

                                                                                                                              aaGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCAAAGATCATTGCCAAGAAc                                                                                                                       >  1:678130/1‑249 (MQ=255)

                                                                                                                               aGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCAAAGATCATTGCCAAGAACg                                                                                                                      >  1:678226/1‑249 (MQ=255)

                                                                                                                                 aCCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCAAAGATCATTGCCAAGAACGaa                                                                                                                    <  2:678095/249‑1 (MQ=255)

                                                                                                                                 aCCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATAACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCAAAGATCATTGCCAAGAACGan                                                                                                                    >  2:678267/1‑248 (MQ=255)

                                                                                                                                      tGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCAAAGATCATTGCCAAGAACGAAGCAgg                                                                                                               >  1:678149/1‑249 (MQ=255)

                                                                                                                                      ngAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCAAAGATCATTGCCAAGAACGAAGCAgg                                                                                                               <  1:678096/248‑1 (MQ=255)

                                                                                                                                        aaGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCAAAGATCATTGCCAAGAACGAAGCAGGCg                                                                                                             >  1:678287/1‑249 (MQ=255)

                                                                                                                                           ataataCCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATGCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCAAAGATCATTGCCAAGAACGAAGCAGGCGTTg                                                                                                          >  2:678285/1‑249 (MQ=255)

                                                                                                                                              ataCCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCAAAGATCATTGCCAAGAACGAAGCAGGCGTTGAAg                                                                                                       <  1:678098/249‑1 (MQ=255)

                                                                                                                                                  cccTGAGGCGCCAGGGTCTTTTACTCCCCTGAAGCTGGAGCAGCCTGGCAGCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCAAAGATCATTGCCAAGAACGAAGCAGGCGTTGAAGCAGa                                                                                                   <  2:678100/249‑1 (MQ=255)

                                                                                                                                                    cTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCAAAGATCATTGCCAAGAACGAAGCAGGCGTTGAAGCAGAAg                                                                                                 >  2:678199/1‑249 (MQ=255)

                                                                                                                                                    cTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCAAAGATCATTGCCAAGAACGAAGCAGGCGTTGAAGCAGAAg                                                                                                 >  2:678229/1‑249 (MQ=255)

                                                                                                                                                    cTGAGGCGCCAGGGGATTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCAAAGATCATTGCCAAGAACGAAGCAGGCGTTGAAGCAGAAg                                                                                                 <  2:678103/249‑1 (MQ=255)

                                                                                                                                                      gAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGTACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCAAAGATCATTGCCAAGAACGAAGCAGGCGTTGAAGCAGAAGtt                                                                                               <  1:678104/249‑1 (MQ=255)

                                                                                                                                                              aGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCAAAGATCATTGCCAAGAACGAAGCAGGCGTTGAAGCAGAAGTTTACGTTaa                                                                                       >  2:678204/1‑249 (MQ=255)

                                                                                                                                                                gggCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCAAAGATCATTGCCAAGAACGAAGCAGGCGTTGAAGCAGAAGTTTACGTTAACt                                                                                     <  1:678106/249‑1 (MQ=255)

                                                                                                                                                                    ntCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCAAAGATCATTGCCAAGAACGAAGCAGGCGTTGAAGCAGAAGTTTACGTTAACTTTGa                                                                                 <  1:678108/248‑1 (MQ=255)

                                                                                                                                                                       naCTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCAAAGATCATTGCCAAGAACGAAGCAGGCGTTGAAGCAGAAGTTTACGTTAACTTTGAttt                                                                              <  1:678111/248‑1 (MQ=255)

                                                                                                                                                                       naCTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCAAAGATCATTGCCAAGAACGAAGCAGGCGTTGAAGCAGAAGTTTACGTTAACTTTGAttt                                                                              <  2:678112/248‑1 (MQ=255)

                                                                                                                                                                         cTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCAAAGATCATTGCCAAGAACGAAGCAGGCGTTGAAGCAGAAGTTTACGTTAACTTTGATTTCa                                                                            <  1:678115/249‑1 (MQ=255)

                                                                                                                                                                         cTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCAAAGATCATTGCCAAGAACGAAGCAGGCGTTGAAGCAGAAGTTTACGTTAACTTTGATTTCa                                                                            >  1:678246/1‑249 (MQ=255)

                                                                                                                                                                             ccTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCAAAGATCATTGCCAAGAACGAAGCAGGCGTTGAAGCAGAAGTTTACGTTAACTTTGATTTCAAcga                                                                        <  1:678117/249‑1 (MQ=255)

                                                                                                                                                                               ngAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCAAAGATCATTGCCAAGAACGAAGCAGGCGTTGAAGCAGAAGTTTACGTTAACTTTGATTTCAAcgaag                                                                      <  1:678119/248‑1 (MQ=255)

                                                                                                                                                                                    cTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCAAAGATCATTGCCAAGAACGAAGCAGGCGTTGAAGCAGAAGTTTACGTTAACTTTGATTTCAAcgaagagcct                                                                 >  1:678253/1‑249 (MQ=255)

                                                                                                                                                                                         gcagcCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCAAAGATCATTGCCAAGAACGAAGCAGGCGTTGAAGCAGAAGTTTACGTTAACTTTGATTTCAAcgaagagccttccag                                                            <  2:678122/249‑1 (MQ=255)

                                                                                                                                                                                              cTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCAAAGATCATTGCCAAGAACGAAGCAGGCGTTGAAGCAGAAGTTTACGTTAACTTTGATTTCAAcgaagagccttccagcgann                                                       >  1:678212/1‑247 (MQ=255)

                                                                                                                                                                                                 gCACCATCGCCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCAAAGATCATTGCCAAGAACGAAGCAGGCGTTGAAGCAGAAGTTTACGTTAACTTTGATTTCAAcgaagagccttccagcgaagagc                                                    <  1:678124/249‑1 (MQ=255)

                                                                                                                                                                                                    ccATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCAAAGATCATTGCCAAGAACGAAGCAGGCGTTGAAGCAGAAGTTTACGTTAACTTTGATTTCAAcgaagagccttccagcgaagagcctt                                                 <  2:678125/249‑1 (MQ=255)

                                                                                                                                                                                                        cACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCAAAGATCATTGCCAAGAACGAAGCAGGCGTTGAAGCAGAAGTTTACGTTAACTTTGATTTCAAcgaagagccttccagcgaagagccttccag                                             >  2:678282/1‑249 (MQ=255)

                                                                                                                                                                                                         aCCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCAAAGATCATTGCCAAGAACGAAGCAGGCGTTGAAGCAGAAGTTTACGTTAACTTTGATTTCAACgaagagccttccagcgaagagccttccagc                                            <  1:678127/249‑1 (MQ=255)

                                                                                                                                                                                                                 cccTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGAAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCAAAGATCATTGCCAAGAACGAAGCAGGCGTTGAAGCAGAAGTTTACGTTAACTTTGATTTCAACGAAGAGCCTTCCAGCGAAGAGCCTTCCAGCGGttcttc                                    >  2:678237/1‑249 (MQ=255)

                                                                                                                                                                                                                 cccTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGAAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCAAAGATCATTGCCAAGAACGAAGCAGGCGTTGAAGCAGAAGTTTACGTTAACTTTGATTTCAACGAAGAGCCTTCCAGCGAAGAGCCTTCCAGCGGttcttc                                    >  2:678255/1‑249 (MQ=255)

                                                                                                                                                                                                                       cAAGGCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCAAAGATCATTGCCAAGAACGAAGCAGGCGTTGAAGCAGAAGTTTACGTTAACTTTGATTTCAACGAAGAGCCTTCCAGCGAAGAGCCTTCCAGCGGTTCTTCCGacac                              >  1:678196/1‑249 (MQ=255)

                                                                                                                                                                                                                          ggCGATCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCAAAGATCATTGCCAAGAACGAAGCAGGCGTTGAAGCAGAAGTTTACGTTAACTTTGATTTCAACGAAGAGCCTTCCAGCGAAGAGCCTTCCAGCGGTTCTTCCGACACTGa                           <  2:678130/249‑1 (MQ=255)

                                                                                                                                                                                                                               tCACCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCAAAGATCATTGCCAAGAACGAAGCAGGCGTTGAAGCAGAAGTTTACGTTAACTTTGATTTCAACGAAGAGCCTTCCAGCGAAGAGCCTGCCAGCGGTTCTTCCGACACTGacaaca                      <  2:678132/249‑1 (MQ=255)

                                                                                                                                                                                                                                 aCCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCAAAGATCATTGCCAAGAACGAAGCAGGCGTTGAAGCAGAAGTTTACGTTAACTTTGATTTCAACGAAGAGCCTTCCAGCGAAGAGCCTTCCAGCGGTTCTTCCGACACTGACAACATc                    <  1:678133/249‑1 (MQ=255)

                                                                                                                                                                                                                                 aCCCCGGTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCAAAGATCATTGCCAAGAACGAAGCAGGCGTTGAAGCAGAAGTTTACGTTAACTTTGATTTCAACGAAGAGCCTTCCAGCGAAGAGCCTTCCAGCGGTTCTTCCGACACTGACAACATc                    >  2:678192/1‑249 (MQ=255)

                                                                                                                                                                                                                                       gTCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCAAAGATCATTGCCAAGAACGAAGCAGGCGTTGAAGCAGAAGTTTACGTTAACTTTGATTTCAACGAAGAGCCTTCCAGCGAAGAGCCTTCCAGCGGTTCTTCCGACACTAACAACATAGAAAAc              >  2:678316/1‑249 (MQ=255)

                                                                                                                                                                                                                                        tCACTGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCAAAGATCATTGCCAAGAACGAAGCAGGCGTTGAAGCAGAAGTTTACGTTAACTTTGATTTCAACGAAGAGCCTTCCAGCGAAGAGCCTTCCAGCGGTTCTTCCGACACTGACAACATCGAAAACt             >  2:678322/1‑249 (MQ=255)

                                                                                                                                                                                                                                            tGTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCAAAGATCATTGCCAAGAACGAAGCAGGCGTTGAAGCAGAAGTTTACGTTAACTTTGATTTCAACGAAGAGCCTTCCAGCGAAGAGCCTTCCAGCGGTTCTTCCGACACTGACAACATCGAAAACTGGAt         >  2:678231/1‑249 (MQ=255)

                                                                                                                                                                                                                                             gTTAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCAAAGATCATTGCCAAGAACGAAGCAGGCGTTGAAGCAGAAGTTTACGTTAACTTTGATTTCAACGAAGAGCCTTCCAGCGAAGAGCCTTCCAGCGGTTCTTCCGACACTGACAACATCGAAAACTGGATc        <  1:678137/249‑1 (MQ=255)

                                                                                                                                                                                                                                               tAAGGTCATCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCAAAGATCATTGCCAAGAACGAAGCAGGCGTTGAAGCAGAAGTTTACGTTAACTTTGATTTCAACGAAGAGCCTTCCAGCGAAGAGCCTTCCAGCGGTTCTTCCGACACTGACAACATCGAAAACTGGATCan      >  2:678210/1‑248 (MQ=255)

                                                                                                                                                                                                                                                 aGGTCATCGCAGGCGAAGCATAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCAAAGATCATTGCCAAGAACGAAGCAGGCGTTGAAGCAGAAGTTTACGTTAACATTGATTTCAACGAAGAGCCTTCCAGCGAAGAGCCTTCCAGCGGTTCTTCCGACACTGACAACATCGAAAACTGGATCAAGa    <  2:678138/249‑1 (MQ=255)

CAGGAAATGAAGCTTTTCTCCCGCACCTCACTGGTTGCACTTGGTACCGCTGCAGCTATCACTCTCTCCGGCGTAACCGCTCCTGCATTCGCTGATGAGGACTCCAACGCAGCTGTAAGTGCTCTTAAGACCGCTGAAGATAATACCCCTGAGGCGCCAGGGGCTTCTACTCCCCTGAAGCTTGAGCAGCCTGGCACCATCACCGGCGTCCCTGGCAAGGCGATCACCCCGGTCACTGTTAAGGTCGTCGCAGGCGAAGCAGAATCCTTCACCTCAGACAACCTTCCTTCTGGTCTCCTTATCGACAACACCGGAAAGATCACCGGTACTCCAAAGAAGGAATTCACTGGTTCCGCAAAGATCATTGCCAAGAACGAAGCAGGCGTTGAAGCAGAAGTTTACGTTAACTTTGATTTCAACGAAGAGCCTTCCAGCGAAGAGCCTTCCAGCGGTTCTTCCGACACTGACAACATCGAAAACTGGATCAAGATC  >  BA000036/1751491‑1751982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`