Predicted mutation
evidence seq id position mutation annotation gene description
RA BA000036 517,141 C→G I216M (ATC→ATG)  Cgl0489 → DNA‑directed RNA polymerase beta' subunit/160 kD subunit (split gene in archaea and Syn)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BA000036517,1410CG100.0% 831.4 / NA 204I216M (ATC→ATG) Cgl0489DNA‑directed RNA polymerase beta' subunit/160 kD subunit (split gene in archaea and Syn)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (92/112);  total (92/112)

Only 100 of 207 total aligned reads displayed.

ATCATCACCAGCGTGGACGAAGAGGCTCGCCACAGCGACCAGACCACTCTTGAGGCAGAAATGCTTCTGGAGAAGAAGGACGTTGAGGCAGACGCAGAGTCTGACATTGCTGAGCGTGCTGAAAAGCTCGAAGAGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATCGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGTCCATTGAGGCTTTGATCCAGAACTTCGACCTTGATGCTGAGGCTGAAGAGCTGCGCGACATCATCAACAATGGCAAGGGCCAGAAGAAGATGCGTGCACTGAAGCGCCTGAAG  >  BA000036/516896‑517384
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   
  catcaCCAGCGTGGACGAAGAGGCTCGCCACAGCGACCAGACCACTCTTGAGGCAGAAATGCTTCTGGAGAAGAAGGACGTTGAGGCAGACGCAGAGTCTGACATTGCTGAGCGTGCTGAAAAGCTCGAAGAGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAatggatcg                                                                                                                                                                                                                                                >  1:220134/1‑249 (MQ=255)
     caCCAGCGTGGACGAAGAGGCTCGCCACAGCGACCAGACCACTCTTGAGGCAGAAATGCTTCTGGAGAAGAAGGACGTTGAGGCAGACGCAGAGTCTGACATTGCTGAGCGTGCTGAAAAGCTCGAAGAGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGtct                                                                                                                                                                                                                                             <  2:220013/249‑1 (MQ=255)
      aCCAGCGTGGACGAAGAGGCTCGCCACAGTGACCAGACCACTCTTGAGGCAGAAATGCTTCTGGAGAAGAAGGACGTTGAGGCAGACGCAGAGTCTGACATTGCTGAGCGTGCTGAAAAGCTCGAAGAGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGtctc                                                                                                                                                                                                                                            <  2:220015/249‑1 (MQ=255)
             tGGACGAAGAGGCTCGCCACAGCGACCAGACCACTCTTGAGGCAGAAATGCTTCTGGAGAAGAAGGACGTTGAGGCAGACGCAGAGTCTGACATTGCTGAGCGTGCTGAAAAGCTCGAAGAGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAgg                                                                                                                                                                                                                                     >  1:220167/1‑249 (MQ=255)
               gACGAAGAGGCTCGCCACAGCGACCAGACCACTCTTGAGGCAGAAATGCTTCTGGAGAAGAAGGACGTTGAGGCAGACGCAGAGTCTGACATTGCTGAGCGTGCTGAAAAGCTCGAAGAGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTc                                                                                                                                                                                                                                   <  1:220016/249‑1 (MQ=255)
                aCGAAGAGGCTCGCCACAGCGACCAGACCACTCTTGAGGCAGAAATGCTTCTGGAGAAGAAGGACGTTGAGGCAGACGCAGAGTCTGACATTGCTGAGCGTGCTGAAAAGCTCGAAGAGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCt                                                                                                                                                                                                                                  <  2:220018/249‑1 (MQ=255)
                  gAAGAGGCTCGCCACAGCGACCAGACCACTCTTGAGGCAGAAATGCTTCTGGAGAAGAAGGACGTTGAGGCAGACGCAGAGTCTGACATTGCTGAGCGTGCTGAAAAGCTCGAAGAGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTgg                                                                                                                                                                                                                                >  1:220143/1‑249 (MQ=255)
                   aaGAGGCTCGCCACAGCGACCAGACCACTCTTGAGGCAGAAATGCTTCTGGAGAAGAAGGACGTTGAGGCAGACGCAGAGTCTGACATTGCTGAGCGTGCTGAAAAGCTCGAAGAGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGc                                                                                                                                                                                                                               <  2:220020/249‑1 (MQ=255)
                            gCCACAGCGACCAGACCACTCTTGAGGCAGAAATGCTTCTGGAGAAGAAGGACGTTGAGGCAGACGCAGAGTCTGACATTGCTGAGCGTGCTGAAAAGCTCGAAGAGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTtca                                                                                                                                                                                                                      <  2:220023/249‑1 (MQ=255)
                             ccACAGCGACCAGACCACTCTTGAGGCAGAAATGCTTCTGGAGAAGAAGGACGTTGAGGCAGACGCAGAGTCTGACATTGCTGAGCGTGCTGAAAAGCTCGAAGAGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTtcat                                                                                                                                                                                                                     >  1:220090/1‑249 (MQ=255)
                              cacaGCGACCAGACCACTCTTGAGGCAGAAATGCTTCTGGAGAAGAAGGACGTTGAGGCAGACGCAGAGTCTGACATTGCTGAGCGTGCTGAAAAGCTCGAAGAGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTtcatc                                                                                                                                                                                                                    <  1:220024/249‑1 (MQ=255)
                                       cagaccaCTCTTGAGGCAGAAATGCTTCTGGAGAAGAAGGACGTTGAGGCAGACGCAGAGTCTGACATTGCTGAGCGTGCTGAAAAGCTCGAAGAGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCt                                                                                                                                                                                                           <  1:220026/249‑1 (MQ=255)
                                        agaccaCTCTTGAGGCAGAAATGCTTCTGGAGAAGAAGGACGTTGAGGCAGACGCAGAGTCTGACATTGCTGAGCGTGCTGAAAAGCTCGAAGAGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTc                                                                                                                                                                                                          >  2:220111/1‑249 (MQ=255)
                                               tctTGAGGCAGAAATGCTTCTGGAGAAGAAGGACGTTGAGGCAGACGCAGAGTCTGACATTGCTGAGCGTGCTGAAAAGCTCGAAGAGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGcn                                                                                                                                                                                                   >  2:220174/1‑248 (MQ=255)
                                               nctTGAGGCAGAAATGCTTCTGGAGAAGAAGGACGTTGAGGCAGACGCAGAGTCTGACATTGCTGAGCGTGCTGAAAAGCTCGAAGAGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCa                                                                                                                                                                                                   <  1:220029/248‑1 (MQ=255)
                                                    aGGCAGAAATGCTTCTGGAGAAGAAGGACGTTGAGGCAGACGCAGAGTCTGACATTGCTGAGCGTGCTGAAAAGCTCGAAGTGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAgatga                                                                                                                                                                                              <  2:220031/249‑1 (MQ=255)
                                                    aGGCAGAAATGCTTCTGGAGAAGAAGGACGTTGAGGCAGACGCAGAGTCTGACATTGCTGAGCGTGCTGAAAAGCTCGAAGAGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAgatga                                                                                                                                                                                              <  1:220032/249‑1 (MQ=255)
                                                     ggCAGAAATGCTTCTGGAGAAGAAGGACGTTGAGGCAGACGCAGAGTCTGACATTGCTGAGCGTGCTGAAAAGCTCGAAGAGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAgatgat                                                                                                                                                                                             >  1:220053/1‑249 (MQ=255)
                                                      gCAGAAATGCTTCTGGAGAAGAAGGACGTTGAGGCAGACGCAGAGTCTGACATTGCTGAGCGTGCTGAAAAGCTCGAAGAGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCTCAAGGTTCCGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGTATCCTCTCGATGAGGTCTGGCAGACCTTCATCAAACTTGCCCCAAAGCAgctgacc                                                                                                                                                                                            >  2:220191/1‑247 (MQ=255)
                                                       cAGAAATGCTTCTGGAGAAGAAGGACGTTGAGGCAGACGCAGAGTCTGACATTGCTGAGCGTGCTGAAAAGCTCGAAGAGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATcc                                                                                                                                                                                           >  2:220132/1‑249 (MQ=255)
                                                       cAGAAATGCTTCTGGAGAAGAAGGACGTTGAGGCAGACGCAGAGTCTGACATTGCTGAGCGTGCTGAAAAGCTCGAAGAGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATcc                                                                                                                                                                                           <  1:220035/249‑1 (MQ=255)
                                                       cAGAAATGCTTCTGGAGAAGAAGGACGTTGAGGCAGACGCAGAGTCTGACATTGCTGAGCGTGCTGAAAAGCTCGAAGAGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATcc                                                                                                                                                                                           >  2:220183/1‑249 (MQ=255)
                                                          aaaTGCTTCTGGAGAAGAAGGACGTTGAGGCAGACGCAGAGTCTGACATTGCTGAGCGTGCTGAAAAGCTCGAAGAGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCcgcg                                                                                                                                                                                        <  2:220039/249‑1 (MQ=255)
                                                          aaaTGCTTCTGGAGAAGAAGGACGTTGAGGCAGACGCAGAGTCTGACATTGCTGAGCGTGCTGAAAAGCTCGAAGAGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCcgcg                                                                                                                                                                                        <  1:220037/249‑1 (MQ=255)
                                                              gCTTCTGGAGAAGAAGGACGTTGAGGCAGACGCAGAGTCTGACATTGCTGAGCGTGCTGAAAAGCTCGAAGAGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATga                                                                                                                                                                                    <  1:220041/249‑1 (MQ=255)
                                                               cTTCTGGAGAAGAAGGACGTTGAGGCAGACGCAGAGTCTGACATTGCTGAGCGTGCTGAAAAGCTCGAAGAGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATgnn                                                                                                                                                                                   >  1:220208/1‑247 (MQ=255)
                                                                ntCTGGAGAAGAAGGACGTTGAGGCAGACGCAGAGTCTGACATTGCTGAGCGTGCTGAAAAGCTCGAAGAGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATgaga                                                                                                                                                                                  <  1:220043/248‑1 (MQ=255)
                                                                      agaagaAGGACGTTGAGGCAGACGCAGAGTCTGACATTGCTGAGCGTGCTGAAAAGCTCGAAGAGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGctct                                                                                                                                                                            >  2:220196/1‑249 (MQ=255)
                                                                           aagGACGTTGAGGCAGACGCAGAGTCTGACATTGCTGAGCGTGCTGAAAAGCTCGAAGAGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGAt                                                                                                                                                                       <  2:220046/249‑1 (MQ=255)
                                                                             ggACGTTGAGGCAGACGCAGAGTCTGACATTGCTGAGCGTGCTGAAAAGCTCGAAGAGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGa                                                                                                                                                                     <  1:220047/249‑1 (MQ=255)
                                                                                   tGAGGCAGACGCAGAGTCTGACATTGCTGAGCGTGCTGAAAAGCTCGAAGAGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGAt                                                                                                                                                               <  1:220049/249‑1 (MQ=255)
                                                                                     aGGCAGACGCAGAGTCTGACATTGCTGAGCGTGCTGAAAAGCTCGAAGAGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCg                                                                                                                                                             >  2:220217/1‑249 (MQ=255)
                                                                                         aGACGCAGAGTCTGACATTGCTGAGCGTGCTGAAAAGCTCGAAGAGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCg                                                                                                                                                         >  2:220138/1‑249 (MQ=255)
                                                                                          gACGCAGAGTCTGACATTGCTGAGCGTGCTGAAAAGCTCGAAGAGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGc                                                                                                                                                        >  1:220283/1‑249 (MQ=255)
                                                                                           aCGCAGAGTCTGACATTGCTGAGCGTGCTGAAAAGCTCGAAGAGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCt                                                                                                                                                       <  2:220051/249‑1 (MQ=255)
                                                                                            cGCAGAGTCTGACATTGCTGAGCGTGCTGAAAAGCTCGAAGAGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTa                                                                                                                                                      >  2:220215/1‑249 (MQ=255)
                                                                                                       aCATTGCTGAGCGTGCTGAAAAGCTCGAAGAGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACt                                                                                                                                           >  2:220261/1‑249 (MQ=255)
                                                                                                         aTTGCTGAGCGTGCTGAAAAGCTCGAAGAGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTc                                                                                                                                         <  1:220056/249‑1 (MQ=255)
                                                                                                         aTTGCTGAGCGTGCTGAAAAGCTCGAAGAGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTc                                                                                                                                         <  2:220055/249‑1 (MQ=255)
                                                                                                           tGCTGAGCGTGCTGAAAAGCTCGAAGAGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCAc                                                                                                                                       <  2:220058/249‑1 (MQ=255)
                                                                                                            gCTGAGCGTGCTGAAAAGCTCGAAGAGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCAcc                                                                                                                                      >  1:220179/1‑249 (MQ=255)
                                                                                                                aGCGTGCTGAAAAGCTCGAAGAGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCggtg                                                                                                                                  <  2:220061/249‑1 (MQ=255)
                                                                                                                  cGTGCTGAAAAGCTCGAAGAGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCggtggt                                                                                                                                <  1:220062/249‑1 (MQ=255)
                                                                                                                   gTGCTGAAAAGCTCGAAGAGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTa                                                                                                                               >  1:220347/1‑249 (MQ=255)
                                                                                                                   gTGCTGAAAAGCTCGAAGAGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGAGGAGAAGCTCTACGATGAACTGATGGACCGCTACGAGGATTACTTCACCGGAGGTa                                                                                                                               >  1:220264/1‑249 (MQ=255)
                                                                                                                      ccgAAAAGCTCGGAGGGGGTCATGCTGGACTTGAGGCAGCTTGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATgg                                                                                                                            <  2:220064/247‑1 (MQ=255)
                                                                                                                       tGAAAAGCTCGAAGAGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATggg                                                                                                                           >  1:220188/1‑249 (MQ=255)
                                                                                                                       ngAAAAGCTCGAAGAGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATggg                                                                                                                           <  2:220066/248‑1 (MQ=255)
                                                                                                                         aaaaGCTCGAAGAGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTg                                                                                                                         >  1:220249/1‑249 (MQ=255)
                                                                                                                               ncGAAGAGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGt                                                                                                                   <  1:220068/248‑1 (MQ=255)
                                                                                                                                  aaGAGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGTCCa                                                                                                                >  1:220378/1‑249 (MQ=255)
                                                                                                                                  aaGAGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGTCCa                                                                                                                >  1:220197/1‑249 (MQ=255)
                                                                                                                                   agagGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGTCCAt                                                                                                               >  1:220203/1‑249 (MQ=255)
                                                                                                                                         tCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGTCCATTGAGGc                                                                                                         >  1:220277/1‑249 (MQ=255)
                                                                                                                                          nntGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGTCCATTGAGGCt                                                                                                        <  1:220072/247‑1 (MQ=255)
                                                                                                                                           ntGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGTCCATTGAGGCtt                                                                                                       <  2:220073/248‑1 (MQ=255)
                                                                                                                                              cTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGTCCATTGAGGCTTTGa                                                                                                    <  2:220076/249‑1 (MQ=255)
                                                                                                                                               tGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGTCCATTGAGGCTTTGAt                                                                                                   >  2:220211/1‑249 (MQ=255)
                                                                                                                                                   cTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGTCCATTGAGGCTTTGATCCAg                                                                                               <  2:220078/249‑1 (MQ=255)
                                                                                                                                                    ttGAGGCAGCTGGCGCCAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGTCCATTGAGGCTTTGATCCAGa                                                                                              >  1:220193/1‑249 (MQ=255)
                                                                                                                                                     tGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGTCCATTGAGGCTTTGATCCAGaa                                                                                             >  2:220125/1‑249 (MQ=255)
                                                                                                                                                       aGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGTCCATTGAGGCTTTGATCCAGAACt                                                                                           <  1:220080/249‑1 (MQ=255)
                                                                                                                                                         gCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGTCCATTGAGGCTTTGATCCAGAACTTc                                                                                         <  2:220081/249‑1 (MQ=255)
                                                                                                                                                             cTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGTCCATTGAGGCTTTGATCCAGAACTTCGAcc                                                                                     >  1:220176/1‑249 (MQ=255)
                                                                                                                                                                gcgcTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGTCCATTGAGGCTTTGATCCAGAACTTCGACCTTg                                                                                  <  1:220084/249‑1 (MQ=255)
                                                                                                                                                                     aaGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGTCCATTGAGGCTTTGATCCAGAACTTCGACCTTGATGCt                                                                             <  2:220086/249‑1 (MQ=255)
                                                                                                                                                                        gCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGTCCATTGAGGCTTTGATCCAGAACTTCGACCTTGATGCTGAg                                                                          <  1:220088/249‑1 (MQ=255)
                                                                                                                                                                          cGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGTCCATTGAGGCTTTGATCCAGAACTTCGACCTTGATGCTGAGGc                                                                        <  2:220089/249‑1 (MQ=255)
                                                                                                                                                                              gCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGTCCATTGAGGCTTTGATCCAGAACTTCGACCTTGATGCTGAGGCTGaa                                                                    <  2:220091/249‑1 (MQ=255)
                                                                                                                                                                               cTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGTCCATTGAGGCTTTGATCCAGAACTTCGACCTTGATGCTGAGGCTGAag                                                                   <  1:220093/249‑1 (MQ=255)
                                                                                                                                                                                  gccgcAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGTCCATTGAGGCTTTGATCCAGAACTTCGACCTTGATGCTGAGGCTGAAGAGc                                                                <  2:220095/249‑1 (MQ=255)
                                                                                                                                                                                     gcAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGTCCATTGAGGCTTTGATCCAGAACTTCGACCTTGATGCTGAGGCTGAAGAGCTgc                                                             >  1:220212/1‑249 (MQ=255)
                                                                                                                                                                                     gcAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGTCCATTGAGGCTTTGATCCAGAACTTCGACCTTGATGCTGAGGCTGAAGAGCTgc                                                             <  1:220096/249‑1 (MQ=255)
                                                                                                                                                                                             cAGGCTGCTGCAGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGTCCATTGAGGCTTTGATCCAGAACTTCGACCTTGATGCTGAGGCTGAAGAGCTGCGCGAcatc                                                     <  2:220099/249‑1 (MQ=255)
                                                                                                                                                                                              aGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGACGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGTCCATTGAGGCTTTGATCCAGAACTTCGACCTTGATGCTGAGGCTGAAGAGCTGCGCGAcatca                                                    >  2:220240/1‑249 (MQ=255)
                                                                                                                                                                                               ggCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGTCCATTGAGGCTTTGATCCAGAACTTCGACCTTGATGCTGAGGCTGAAGAGCTGCGCGAcatcat                                                   <  1:220100/249‑1 (MQ=255)
                                                                                                                                                                                                 ctgctgCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGTCCATTGAGGCTTTGATCCAGAACTTCGACCTTGATGCTGAGGCTGAAGAGCTGCGCGACAtcatca                                                 >  2:220159/1‑249 (MQ=255)
                                                                                                                                                                                                  tgctgcCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGTCCATTGAGGCTTTGATCCAGAACTTCGACCTTGATGCTGAGGCTGAAGAGCTGCGCGACATCATcaa                                                >  2:220224/1‑249 (MQ=255)
                                                                                                                                                                                                       ccGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGTCCATTGAGGCTTTGATCCAGAACTTCGACCTTGATGCTGAGGCTGAAGAGCTGCGCGACATCATCAACAATg                                           <  2:220102/249‑1 (MQ=255)
                                                                                                                                                                                                       ccGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGTCCATTGAGGCTTTGATCCAGAACTTCGACCTTGATGCTGAGGCTGAAGAGCTGCGCGACATCATCAACAATg                                           <  1:220103/249‑1 (MQ=255)
                                                                                                                                                                                                        cGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGTCCATTGAGGCTTTGATCCAGAACTTCGACCTTGATGCTGAGGCTGAAGAGCTGCGCGACATCATCAACAATgg                                          >  2:220268/1‑249 (MQ=255)
                                                                                                                                                                                                              ggAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGTCCATTGAGGCTTTGATCCAGAACTTCGACCTTGATGCTGAGGCTGAAGAGCTGCGCGACATCATCAACAATGGCAAggg                                    <  1:220105/249‑1 (MQ=255)
                                                                                                                                                                                                                aaaTGCAGCACATCCGTGAGCGTGCACAGCGCGAAATGTATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGTCCATTGAGGCTTTGATCCAGAACTTCGACCTTGATGCTGAGGCTGAAGAGCTGCGCGACATCATCAACAATGGCAAGGGcc                                  <  2:220107/249‑1 (MQ=255)
                                                                                                                                                                                                                    gcagcaCATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGTCCATTGAGGCTTTGATCCAGAACTTCGACCTTGATGCTGAGGCTGAAGAGCTGCGCGACATCATCAACAATGGCAAGGGCCagan                              >  1:220220/1‑248 (MQ=255)
                                                                                                                                                                                                                     cagcaCATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGTCCATTGAGGCTTTGATCCAGAACTTCGACCTTGATGCTGAGGCTGAAGAGCTGCGCGACATCATCAACAATGGCAAGGGCCagann                             >  2:220319/1‑247 (MQ=255)
                                                                                                                                                                                                                      agcaCATCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGTCCATTGAGGCTTTGATCCAGAACTTCGACCTTGATGCTGAGGCTGAAGAGCTGCGCGACATCATCAACAATGGCAAGGGCCagaaga                            <  1:220109/249‑1 (MQ=255)
                                                                                                                                                                                                                         acaTCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGTCCATTGAGGCTTTGATCCAGAACTTCGACCTTGATGCTGAGGCTGAAGAGCTGCGCGACATCATCAACAATGGCAAGGGCCagaagannn                         >  1:220273/1‑246 (MQ=255)
                                                                                                                                                                                                                         acaTCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGTCCATTGAGGCTTTGATCCAGAACTTCGACCTTGATGCTGAGGCTGAAGAGCTGCGCGACATCATCAACAATGGCAAGCGCCagaagannn                         >  2:220295/1‑246 (MQ=255)
                                                                                                                                                                                                                          caTCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGTCCATTGAGGCTGTGATCCAGAACTTCGACCTTGATGCTGAGGCTGAAGAGCTGCGCGACATCATCAACAATGGCAAGGGCCAGAAGAAGAt                        >  1:220313/1‑249 (MQ=255)
                                                                                                                                                                                                                            tCCGTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGTCCATTGAGGCTTTGATCCAGAACTTCGACCTTGATGCTGAGGCTGAAGAGCTGCGCGACATCATCAACAATGGCAAGGGCCAGAAGAAGATGc                      <  1:220111/249‑1 (MQ=255)
                                                                                                                                                                                                                               gTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGTCCATTGAGGCTTTGATCCAGAACTTCGACCTTGATGCTGAGGCTGAAGAGCTGCGCGACATCATCAACAATGGCAAGGGCCAGAAGAAGATGCGTg                   <  2:220113/249‑1 (MQ=255)
                                                                                                                                                                                                                               gTGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGTCCATTGAGGCTTTGATCCAGAACTTCGACCTTGATGCTGAGGCTGAAGAGCTGCGCGACATCATCAACAATGGCAAGGGCCAGAAGAAGATGCGTg                   >  1:220234/1‑249 (MQ=255)
                                                                                                                                                                                                                                tGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGTCCATTGAGGCTTTGATCCAGAACTTCGACCTTGATGCTGAGGCTGAAGAGCTGCGCGACATCATCAACAATGGCAAGGGCCAGAAGAAGATGCGTGc                  >  2:220201/1‑249 (MQ=255)
                                                                                                                                                                                                                                tGAGCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGTCCATTGAGGCTTTGATCCAGAACTTCGACCTTGATGCTGAGGCTGAAGAGCTGCGCGACATCATCAACAATGGCAAGGGCCAGAAGAAGATGCGTGc                  <  1:220114/249‑1 (MQ=255)
                                                                                                                                                                                                                                   gCGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGTCCATTCAGGCTTTGATCCAGAACTTCGACCTTGATGCTGAGGCTGAAGAGCTGCGCGACATCATCAACAATGGCAAGGGCCAGAAGAAGATGCGTGCACt               <  2:220116/249‑1 (MQ=255)
                                                                                                                                                                                                                                    cGTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGTCCATTGAGGCTTTGATCCAGAACTTCGACCTTGATGCTGAGGCTGAAGAGCTGCGCGACATCATCAACAATGGCAAGGGCCAGAAGAAGATGCGTGCACTg              >  2:220154/1‑249 (MQ=255)
                                                                                                                                                                                                                                     gTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGTCCATTGAGGCTTTGATCCAGAACTTCGACCTTGATGCTGAGGCTGAAGAGCTGCGCGACATCATCAACAATGGCAAGGGCCAGAAGAAGATGCGTGCACTGa             <  1:220118/249‑1 (MQ=255)
                                                                                                                                                                                                                                     gTGCACAGCGCGAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGTCCATTGAGGCTTTGATCCAGAACTTCGACCTTGATGCTGAGGCTGAAGAGCTGCGCGACATCATCAACAATGGCAAGGGCCAGAAGAAGATGCGTGCACTGa             <  2:220119/249‑1 (MQ=255)
                                                                                                                                                                                                                                              gcgAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGTCCATTGAGGCTTTGATCCAGAACTTCGACCTTGATGCTGAGGCTGAAGAGCTGCGCGACATCATCAACAATGGCAAGGGCCAGAAGAAGATGCGTGCACTGAAGCGCCTGa    <  2:220122/249‑1 (MQ=255)
                                                                                                                                                                                                                                                gAAATGGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGTCCATTGAGGCTTTGATCCAGAACTTCGACCTTGATGCTGAGGCTGAAGAGCTGCGCGACATCATCAACAATGGCAAGGGCCAGAAGAAGATGCGTGCACTGAAGCGCCTGAAg  <  1:220124/249‑1 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   
ATCATCACCAGCGTGGACGAAGAGGCTCGCCACAGCGACCAGACCACTCTTGAGGCAGAAATGCTTCTGGAGAAGAAGGACGTTGAGGCAGACGCAGAGTCTGACATTGCTGAGCGTGCTGAAAAGCTCGAAGAGGATCTTGCTGAACTTGAGGCAGCTGGCGCTAAGGCCGACGCTCGCCGCAAGGTTCAGGCTGCTGCCGATAAGGAAATGCAGCACATCCGTGAGCGTGCACAGCGCGAAATCGATCGTCTCGATGAGGTCTGGCAGACCTTCATCAAGCTTGCTCCAAAGCAGATGATCCGCGATGAGAAGCTCTACGATGAACTGATCGACCGCTACGAGGATTACTTCACCGGTGGTATGGGTGCAGAGTCCATTGAGGCTTTGATCCAGAACTTCGACCTTGATGCTGAGGCTGAAGAGCTGCGCGACATCATCAACAATGGCAAGGGCCAGAAGAAGATGCGTGCACTGAAGCGCCTGAAG  >  BA000036/516896‑517384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑