Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 3,728,808 (C)10→8 pseudogene (1254‑1255/1457 nt) xylB ← xylulokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,728,8000C.91.8% 486.5 / 34.3 123pseudogene (1263/1457 nt)xylBxylulokinase
Reads supporting (aligned to +/- strand):  ref base C (8/2);  new base . (50/62);  total (59/64)
Fisher's exact test for biased strand distribution p-value = 4.61e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NZ_CP0252683,728,8010C.90.2% 390.3 / 61.9 123pseudogene (1262/1457 nt)xylBxylulokinase
Reads supporting (aligned to +/- strand):  ref base C (3/1);  major base . (43/58);  minor base G (8/3);  total (59/64)
Fisher's exact test for biased strand distribution p-value = 1.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 139 total aligned reads displayed.

GGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCA  >  NZ_CP025268/3728661‑3728933
                                                                                                                                           ||                                                                                                                                    
ggCTGATTAGCGGCATATCGCTGCGCATCTGGTAGATGCGACTGTTCGAACGGTAGTTGCGGCAACAATTCAATGAGCGATGTCTCTGGCTTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATc                                                                                                                                       <  2:970277/140‑1 (MQ=255)
ggCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTc                                                                                                                                       >  2:1493462/1‑140 (MQ=255)
  cTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccc                                                                                                                                     <  2:5848985/140‑1 (MQ=255)
  cTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccc                                                                                                                                     <  2:5848997/140‑1 (MQ=255)
     aTAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATcccccc                                                                                                                                  <  2:937549/140‑1 (MQ=255)
     aTAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATcccccc                                                                                                                                  <  1:1669432/140‑1 (MQ=255)
        aGCGGCATAACGCTGCTCATCTTGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAAAAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccg                                                                                                                               <  2:7302726/140‑2 (MQ=255)
        aGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccg                                                                                                                               <  2:4656056/140‑2 (MQ=255)
         gCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGACAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccgg                                                                                                                              >  2:7014034/1‑138 (MQ=255)
          cGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTcgcccccggtg                                                                                                                             >  2:178529/1‑136 (MQ=255)
           ggCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccgtac                                                                                                                            <  1:6960333/140‑5 (MQ=255)
           ggCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccgtac                                                                                                                            <  1:6960390/140‑5 (MQ=255)
           ggCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccgtac                                                                                                                            <  2:6193188/140‑5 (MQ=255)
                  cGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCCGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATc                                                                                                                   <  2:2736750/140‑1 (MQ=255)
                  cGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATc                                                                                                                   >  1:1553054/1‑140 (MQ=255)
                     tGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAg                                                                                                                <  1:2623756/140‑1 (MQ=255)
                      gcgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAgc                                                                                                               >  2:7548804/1‑140 (MQ=255)
                       cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CGCCGCCCCCGTACGGGAGTCGGgct                                                                                                              >  1:3083817/1‑140 (MQ=255)
                       cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGACCAGTGCTGGCCCCACAT‑‑CGCCCCCCGGACGGTTATCGAgcg                                                                                                              >  1:3313642/1‑139 (MQ=255)
                        gcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAgctg                                                                                                             <  2:4930762/140‑1 (MQ=255)
                          aTCTGGTAGATGCGACTGTTCTACCGGTAGTGGCGGCCACAATTCAATGAGCGATTACTCTGGATTCGCCGCGAACTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGTACGGTAATCGAgctgct                                                                                                           <  2:5465202/140‑1 (MQ=255)
                          aTCTGGTAGATGCGACTGTTCTAACTGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAgctgct                                                                                                           <  1:4556183/140‑1 (MQ=255)
                          aTCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAgctgct                                                                                                           <  1:2401163/140‑1 (MQ=255)
                          aTCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAgctgct                                                                                                           <  2:6565216/140‑1 (MQ=255)
                            cTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGa                                                                                                         <  2:224691/140‑1 (MQ=255)
                                    tGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGGACGGGAGTCGAGCTGCGGACCGcgggt                                                                                                 >  1:5435506/1‑136 (MQ=255)
                                        aCTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCCCCCCGGT‑CGGTACTCGAGCTGCGGTCCGCTggtctgc                                                                                             >  2:6862195/1‑134 (MQ=255)
                                          tGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGc                                                                                           <  1:125152/140‑1 (MQ=255)
                                            ttCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCCC‑‑CTGCCCCCCCGTGCGGTATTCGCGGTGCTGTCCGGGGAGGTCCGccg                                                                                         >  2:8505025/1‑139 (MQ=255)
                                            ttCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCa                                                                                         <  2:3218496/140‑1 (MQ=255)
                                             tCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAg                                                                                        <  1:3038541/140‑1 (MQ=255)
                                             tCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAg                                                                                        <  2:3560198/140‑1 (MQ=255)
                                              cTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGGACGGTCAGCGAGCTGCGGACCGCTGCTATCCGCCAGc                                                                                       >  2:9063386/1‑140 (MQ=255)
                                                  cGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                   <  1:3689184/140‑1 (MQ=255)
                                                    gTAGTTGCGGCAACACTTCAATGAGCGATTTCTCGGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCCGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTCACCGAGCTGCGGACCGCTGATATCCGCCAGCATCTGa                                                                                 <  1:169297/140‑1 (MQ=255)
                                                    gTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                                 <  1:5625171/140‑1 (MQ=255)
                                                    gTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                                 <  1:6124919/140‑1 (MQ=255)
                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCGCAT‑‑CCCCCCCCGTACGGTAATCGAGGTGCTGTCCGCTGGTATCCGGCAGCGTCTGTCg                                                                               >  1:1846926/1‑140 (MQ=255)
                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑GCCCCCCCGTACGGTATGGGTGCTGGTGACGGCGGTTGTCCGCCAGCAGCTGACg                                                                               >  1:131614/1‑140 (MQ=255)
                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTGCGGTAAGCGGGCTGGTGTCCGGTGAGATCCGCCAGCATCTGTCg                                                                               >  2:1647544/1‑140 (MQ=255)
                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                               <  2:1553054/140‑1 (MQ=255)
                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCACCCAGTACGGTAATAGAGCTGCTGACAGCGGATATCCGCCAGCATCTGACg                                                                               >  2:2772164/1‑140 (MQ=255)
                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑GCCCCCCCCGTCCGGTAAGCGTGCTGCTGACCGCTGGGAGCCGCCCGCAGCTGACg                                                                               >  2:3233932/1‑140 (MQ=255)
                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CGCCCCCCCCGTCCGGGAATCGAGCTGCGGACCGCGGTGTGCCGCCAGCATCTGACg                                                                               >  1:3444893/1‑140 (MQ=255)
                                                      aGTTGCGGCAACAATTCAATGAGCGATGTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                               <  1:8601610/140‑1 (MQ=255)
                                                       gTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGc                                                                              <  2:7301577/140‑1 (MQ=255)
                                                       gTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGc                                                                              <  1:9428891/140‑1 (MQ=255)
                                                       gTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGc                                                                              <  2:8751326/140‑1 (MQ=255)
                                                        ttGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGcc                                                                             <  1:7728245/140‑1 (MQ=255)
                                                        ttGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGcc                                                                             <  1:5186229/140‑1 (MQ=255)
                                                         tGCGGCAACAATTCAATGAGCGAGTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCa                                                                            <  2:5560323/140‑1 (MQ=255)
                                                           cGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCCCAT‑‑CCCCCCCCGTACGGGAATCGAGCTGCTGACCGCTGAGATCCGCCAGCAGCGGCCGCCAgg                                                                          >  2:7938371/1‑139 (MQ=255)
                                                             gCAACAATTCAATGAGCGATTTCTCTGGATTCGACGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCGACAT‑‑CCACCCCCGAACGGTACTCGAGCTGCTGACCGATGATTTCCGGCAGAGTCTGAGGGCAGtcc                                                                        >  2:7924456/1‑138 (MQ=255)
                                                               aacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTc                                                                      <  1:7511780/140‑1 (MQ=255)
                                                                    ttCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTAc                                                                 <  1:906062/140‑1 (MQ=255)
                                                                        aTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGTCGCCAGTACTGACTACgcgc                                                             >  2:5808532/1‑140 (MQ=255)
                                                                         tGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGGACGGTCATCGAGGGGGTGGCCGCGGAGAGCCGCCCGCATCTGGCGCCAGTGCTCGCTACGcgcc                                                            >  2:1847533/1‑140 (MQ=255)
                                                                            gCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTCCGGGAATCGAGCTGCTGACCGCTGAGATCCGGCAGGATCTGACGCCGGGACTCACGAGGcgccccg                                                         >  2:4176856/1‑140 (MQ=255)
                                                                            gCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccg                                                         <  1:7186791/140‑1 (MQ=255)
                                                                             cGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCTCCAGCCTCTGACGCCAGTACTCACTACGcgccccgc                                                        <  2:241475/140‑1 (MQ=255)
                                                                              gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGATGACCGCTGATCTCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccgcc                                                       >  2:6090093/1‑140 (MQ=255)
                                                                                  tctcTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCa                                                   <  2:5872689/140‑1 (MQ=255)
                                                                                  tctcTGGAGTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTACTCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCa                                                   <  2:1123277/140‑1 (MQ=255)
                                                                                    tctTGATACGCCGCGTTCTGCGCCACCCTAGCTGCGCCCCGTGCTGTCCCCACAT‑‑CCCCCACCGTCCTGTAATCGAGCTGCTGACCGCTTATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                                 <  2:3511433/140‑1 (MQ=255)
                                                                                    tctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                                 <  1:8819569/140‑1 (MQ=255)
                                                                                        gATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGGGCGGGACTGGAGCTGCTGCCCGCTGGTCGCGGCCCGGCTCGGACGCCAGTCCGCAGGACGGGGCCCGcccccccgccac                                             >  2:282764/1‑133 (MQ=255)
                                                                                          ttCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTGCGGTAATCGAGCTGCTGACCGCTGTTATCCGCCAGCGTCTGTCGCCTGTACTGTCTACGCGCCCCGCGCCCGATCAACGt                                           >  1:5251222/1‑140 (MQ=255)
                                                                                             gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGGTATCCGCCAGCATCTGACGCCCGTACTCACTGCGCGCCCCGCCCCCAAGCGACGTCac                                        >  1:216639/1‑140 (MQ=255)
                                                                                             gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGATATCTATACGCGCGCCTCGCCGAATCAACGTaag                                        >  2:5982009/1‑139 (MQ=255)
                                                                                             gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGGACGGTCATCGAGCTGCTGTCCGCGGGGATCCGCCAGCATCTGACGCCAGTCCTCACTACGCGCCCCGCCCCCCATCAACgggac                                        >  1:3788508/1‑136 (MQ=255)
                                                                                              ccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                   >  2:7729870/1‑96 (MQ=255)
                                                                                              ccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                   <  1:7729870/96‑1 (MQ=255)
                                                                                                   aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                                  <  2:7661880/140‑1 (MQ=255)
                                                                                                   aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                                  <  1:913587/140‑1 (MQ=255)
                                                                                                   aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGAACGGTAAACGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                                  <  1:9051777/140‑1 (MQ=255)
                                                                                                        cgcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTCAGCGAGCTGCTGACCGATGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCACTCAACGTAACACTTTGCGGtt                             <  2:5117939/140‑1 (MQ=255)
                                                                                                        cgcCAGCCTTGCTGCGCCCAGTGCTGGCCACACA‑‑GCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGtt                             <  2:5117998/140‑1 (MQ=255)
                                                                                                                ttGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCACCGTAACACTTTGCGGTTTAATACCg                     <  1:7603005/140‑1 (MQ=255)
                                                                                                                ttGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCg                     <  1:3701803/140‑1 (MQ=255)
                                                                                                                ttGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCg                     <  1:2985844/140‑1 (MQ=255)
                                                                                                                  gctgcGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTc                                                                      >  1:1166861/1‑89 (MQ=255)
                                                                                                                  gctgcGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCa                   <  2:4902331/140‑1 (MQ=255)
                                                                                                                  gctgcGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATAACGCa                   >  2:3296760/1‑140 (MQ=255)
                                                                                                                  gctgcGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGGCCTCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGGACTCACTCCGCGCCCCGCCCCCACTCAACGGAACGCTTTGCGGTTTAATACCGCa                   >  1:8343787/1‑140 (MQ=255)
                                                                                                                      cgcCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCa               <  1:372636/140‑1 (MQ=255)
                                                                                                                      cgcCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCa               <  1:6756088/140‑1 (MQ=255)
                                                                                                                        cccAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTTCTGACTACGGGCCCCGCCCCCGATCAACGTATCACGGTGCGGGTGAATACCGCAGGCATg             >  1:8184500/1‑140 (MQ=255)
                                                                                                                        cccAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCGGATATCCGCCAGCATCTGACGCCAGTACTCACGACGCGCCCCGCCCCCAATCCACGGACCCCTTTGCGGTTTAATACCGCAGGCATg             >  1:8184410/1‑140 (MQ=255)
                                                                                                                         ccAGTGCTGGCCCCACCT‑CCCCCCCCGGT‑CGGTAATCGGGCTGCTGACCGCTGATGTCCGCCAGCCTCTGACGCCAGTCCTCACTGCGCGCCCCGCCCCCAATCAACGTAACACTGTGCGGGTTAATACCGCaggcaggc            >  2:7776593/1‑140 (MQ=255)
                                                                                                                          cAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCa           <  1:4955251/140‑1 (MQ=255)
                                                                                                                          cAGTGCTGGCCCCACA‑‑GCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCa           <  1:4955443/140‑1 (MQ=255)
                                                                                                                           aGTGCTGGCCCCACAT‑‑CCCCCCCCGTTCGGTGTTCGGGCTGGTGGCCGCTGTGAGCCGCCAGCATCTGTCGCCCGTGCTCACTACGCGGCCCGCCCCCCATCAACGTGCCACTTTGCGGGTTAGTGCCGCAGGCATGctc          >  2:4142482/1‑138 (MQ=255)
                                                                                                                            gTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACg         <  1:1849320/140‑1 (MQ=255)
                                                                                                                             tGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTCCTCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCTACGTAACACTTTGCGGTTTAATAACGCAGGCATGCACgc        >  2:9146707/1‑139 (MQ=255)
                                                                                                                             tGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTACTCGAGCTGCTGTCCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTCCGCGCCCCGCCCCCAAGCAACGTAACACGTTGCGGTTTAATACCGCTGGCATGCACGa        >  2:6162062/1‑140 (MQ=255)
                                                                                                                              gCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGAc       <  2:3371290/140‑1 (MQ=255)
                                                                                                                                  gCCCCACAT‑‑CCCCCCCCGTACGGTAATCGCGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTGAATACCGCAGGCATGCACGGCATcc   >  1:2226818/1‑140 (MQ=255)
                                                                                                                                  gCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCCGCATCTGCCGCCAGTACTCACTTCGCGCCCCGCCCCCAATCCACGATACACTTAGCGGTTTAATACCGCAGGCCTGCACGACATcc   >  2:8853898/1‑140 (MQ=255)
                                                                                                                                  gCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAGCAACGTAACACTTTGCGGGTGGAGACCGCAGGCATGCACGACATcc   >  1:2075171/1‑140 (MQ=255)
                                                                                                                                   ccccACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCa  >  1:4647404/1‑140 (MQ=255)
                                                                                                                                           ||                                                                                                                                    
GGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCA  >  NZ_CP025268/3728661‑3728933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑