Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 3,982,878 (G)8→6 pseudogene (72‑73/1388 nt) yifK → amino acid permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,982,8720G.99.8% 2452.5 / ‑3.7 507pseudogene (66/1388 nt)yifKamino acid permease
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base . (258/248);  total (259/248)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NZ_CP0252683,982,8730G.98.6% 2398.6 / 16.2 507pseudogene (67/1388 nt)yifKamino acid permease
Reads supporting (aligned to +/- strand):  ref base G (6/1);  new base . (253/247);  total (259/248)
Fisher's exact test for biased strand distribution p-value = 1.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 543 total aligned reads displayed.

CATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAAT  >  NZ_CP025268/3982733‑3983005
                                                                                                                                           ||                                                                                                                                    
     gCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTgggggg                                                                                                                                  <  2:243693/140‑1 (MQ=255)
      cAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTggggggc                                                                                                                                 <  1:9372855/140‑2 (MQ=255)
           cTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTggggggcaccat                                                                                                                            >  1:4032401/1‑134 (MQ=255)
           cTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTggggggcaccat                                                                                                                            <  1:4876751/140‑7 (MQ=255)
                tGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCg                                                                                                                     <  1:7994403/140‑1 (MQ=255)
                tGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCg                                                                                                                     >  2:4146401/1‑140 (MQ=255)
                tGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCg                                                                                                                     <  2:8552381/140‑1 (MQ=255)
                 gTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGt                                                                                                                    <  2:3875627/140‑1 (MQ=255)
                   aCCCATAACGATCACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCg                                                                                                                  >  2:2134880/1‑140 (MQ=255)
                   aCCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCg                                                                                                                  >  1:4779650/1‑140 (MQ=255)
                   aCCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCg                                                                                                                  >  1:983659/1‑140 (MQ=255)
                   aCCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCg                                                                                                                  >  1:5657336/1‑140 (MQ=255)
                    cccATCACGATAACCGGTAACACCGGACAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCgg                                                                                                                 <  1:5386848/140‑1 (MQ=255)
                    cccATAACGATAACCGGTAACACCGGAAAGCATGCAAACCCACCACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCgg                                                                                                                 <  2:3927285/140‑1 (MQ=255)
                    cccATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGTCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCgg                                                                                                                 >  2:3519173/1‑140 (MQ=255)
                    cccATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCATATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCgg                                                                                                                 <  2:1694110/140‑1 (MQ=255)
                    cccATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCgg                                                                                                                 >  1:7221597/1‑140 (MQ=255)
                     ccATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGc                                                                                                                <  1:3606576/140‑1 (MQ=255)
                     ccATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATGTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGc                                                                                                                <  2:3313394/140‑1 (MQ=255)
                      cATAACGATAACCGGGAACCCCGGATAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGcc                                                                                                               <  2:9154641/140‑1 (MQ=255)
                         aaCGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCgggcggt                                                                                                            >  1:7781879/1‑135 (MQ=255)
                         aaCGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGt                                                                                                            >  1:2966345/1‑140 (MQ=255)
                          aCGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCg                                                                                                                     >  1:8991837/1‑130 (MQ=255)
                          aCGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGtt                                                                                                           >  2:2680800/1‑140 (MQ=255)
                             aTAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTAt                                                                                                        >  1:5251322/1‑140 (MQ=255)
                              tAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGATGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCGGTTTATg                                                                                                       >  2:4756512/1‑140 (MQ=255)
                              tAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATCTCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTGATg                                                                                                       >  2:9040186/1‑140 (MQ=255)
                              tAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATg                                                                                                       >  1:6007311/1‑140 (MQ=255)
                              tAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATg                                                                                                       >  1:8773474/1‑140 (MQ=255)
                              tAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATg                                                                                                       <  1:4637361/140‑1 (MQ=255)
                              tAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTGTATg                                                                                                       <  1:6188248/140‑1 (MQ=255)
                              tAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGGTTCTg                                                                                                       >  1:756644/1‑140 (MQ=255)
                              tAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGAAATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGGCGGTTTCTg                                                                                                       >  2:5001644/1‑140 (MQ=255)
                              tAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGATCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGTCGTCGGCGTGTTGATg                                                                                                       >  2:8799897/1‑140 (MQ=255)
                              tAACCGGAAACACCGGACAGCATGCAAACACAACACGAGGATATATGGCAGATAACAAACCAGAGCTACAGAGTGGGCTGGAAGCTCGACATAAAGAACTCATCGACCT‑‑GGGGGGCACCATTGGACTCTGCCTCGTTATg                                                                                                       >  2:9478071/1‑140 (MQ=255)
                               aaCCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATgg                                                                                                      >  1:6845310/1‑140 (MQ=255)
                                 ccGGTAACACCGGACAGCATGCAACCACAACACGAGGAGTTAGTGCAGATACCCCACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATCTCGAACTCAGCGCCCT‑‑GGGGGGCACCAGTGGCGTCGGCCTGTTTATgggg                                                                                                    <  2:579683/140‑1 (MQ=255)
                                 ccGGTAACACCGGAAAGCATGCAAACACAACCCGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATgggg                                                                                                    <  2:4519640/140‑1 (MQ=255)
                                 ccGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATgggg                                                                                                    <  2:7812194/140‑1 (MQ=255)
                                     tAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGgccg                                                                                                >  2:3749809/1‑140 (MQ=255)
                                     tAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGgccg                                                                                                >  2:4875746/1‑140 (MQ=255)
                                        cacCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa                                                                                             <  1:7661847/140‑1 (MQ=255)
                                           cGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTa                                                                                          >  1:7085392/1‑140 (MQ=255)
                                           cGGAAAGCATGCAAACACAACACGAGGAGTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTa                                                                                          >  2:5533767/1‑140 (MQ=255)
                                             gAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTAcc                                                                                        <  1:6422144/140‑1 (MQ=255)
                                                  cATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGaa                                                                                   <  1:8354193/140‑1 (MQ=255)
                                                   aTGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGaaa                                                                                  >  1:2386678/1‑140 (MQ=255)
                                                   aTGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGaaa                                                                                  <  1:7336797/140‑1 (MQ=255)
                                                         acacaacacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGGTTATGGGGGCCGCCAGTACCCTGAAATgggcc                                                                            >  1:9247652/1‑140 (MQ=255)
                                                            caacacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccggg                                                                         >  1:1157858/1‑140 (MQ=255)
                                                            caacacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccggg                                                                         >  1:3945504/1‑140 (MQ=255)
                                                             cccacGAGGATTTCTGGGAGATAACAATCCCGTCCTTCCGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgggc                                                                        <  1:4400669/138‑1 (MQ=255)
                                                             aacacGAGGATTTATGGCAGATAACAACCCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgggc                                                                        <  1:2550951/140‑1 (MQ=255)
                                                             aacacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAACTgggccgggc                                                                        <  2:734896/140‑1 (MQ=255)
                                                             aacacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgggc                                                                        <  1:1604579/140‑1 (MQ=255)
                                                              acacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGACGCCAGTACCCTGAAATgggccgggcc                                                                       <  1:2768602/140‑1 (MQ=255)
                                                               cacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATCTCGAACTCCTCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTGTAGGGGGGCCGCCAGTACCCTGAAATgggccgggccc                                                                      >  2:7732022/1‑139 (MQ=255)
                                                                ccGAGGAGTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGACCTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCAt                                                                     <  2:3135846/139‑1 (MQ=255)
                                                                acGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCAt                                                                     <  2:1977740/140‑1 (MQ=255)
                                                                acGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCAt                                                                     <  2:7505569/140‑1 (MQ=255)
                                                                   aGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCg                                                                  <  1:4259518/140‑1 (MQ=255)
                                                                   aGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCg                                                                  <  1:6572432/140‑1 (MQ=255)
                                                                   aGGATTTATGGCAGAGAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGACCTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCg                                                                  <  2:1394259/140‑1 (MQ=255)
                                                                    ggATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGt                                                                 >  1:6161907/1‑140 (MQ=255)
                                                                     gATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTa                                                                >  1:1908813/1‑140 (MQ=255)
                                                                        ttctGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttg                                                             <  1:5813222/137‑1 (MQ=255)
                                                                        ttATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCTCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttg                                                             <  1:6968111/140‑1 (MQ=255)
                                                                        ttATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttg                                                             <  1:3340969/140‑1 (MQ=255)
                                                                        ttATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttg                                                             <  1:8537624/140‑1 (MQ=255)
                                                                           tGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttgttg                                                          <  1:2159222/140‑1 (MQ=255)
                                                                             gCAGATAACAAACCAGAGCTACAGCGTGGGCTGGCAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCTCCAGTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCTGGCCATCCGTATTGTTGGc                                                        <  2:430678/140‑1 (MQ=255)
                                                                             gCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGc                                                        <  2:8446438/140‑1 (MQ=255)
                                                                             gCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGc                                                        <  1:4197748/140‑1 (MQ=255)
                                                                              cAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGcc                                                       <  2:8311994/140‑1 (MQ=255)
                                                                              cAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGcc                                                       <  2:723594/140‑1 (MQ=255)
                                                                              cAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGcc                                                       <  1:1295148/140‑1 (MQ=255)
                                                                               aGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCt                                                      <  2:6773706/140‑1 (MQ=255)
                                                                               aGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCt                                                      <  2:7941354/140‑1 (MQ=255)
                                                                                gATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCta                                                     >  2:6602927/1‑140 (MQ=255)
                                                                                gATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCt                                                      >  1:1733262/1‑139 (MQ=255)
                                                                                   aaCAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTatat                                                  <  1:3530870/140‑1 (MQ=255)
                                                                                           agagCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCg                                          >  2:8971090/1‑140 (MQ=255)
                                                                                           agagCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCg                                          <  2:5377228/140‑1 (MQ=255)
                                                                                             agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCggg                                        >  2:1906119/1‑140 (MQ=255)
                                                                                             agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCggg                                        >  1:5036315/1‑140 (MQ=255)
                                                                                             agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCggg                                        <  1:8128549/140‑1 (MQ=255)
                                                                                              gCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGc                                       <  2:402259/140‑1 (MQ=255)
                                                                                                  cAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGtt                                   >  1:3754580/1‑140 (MQ=255)
                                                                                                       tGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCt                              <  2:6248414/140‑1 (MQ=255)
                                                                                                       tGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCt                              >  2:7128141/1‑140 (MQ=255)
                                                                                                       tGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATGGTTGGCCTATATCATCGCCGGGCTGTTCGTCt                              >  2:5906921/1‑140 (MQ=255)
                                                                                                         ggCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCttt                            >  2:2924614/1‑140 (MQ=255)
                                                                                                         ggCTGGAAGCTCGACATATCGAACTAATCTACCT‑‑GGTGGGCACCATTTGCGTCTGACTGTTTATGGGTGACGCCAGTACCATGAAATGGGCCGTGACATCCGTATTGTTGGCCTATATCATAGCCGGGCTGTTCTTCttt                            >  2:1226318/1‑140 (MQ=255)
                                                                                                               aaGCTCGACATATCGAACTCATCGCGC‑‑GGGGGGGCCCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTtcatc                      >  1:6753017/1‑140 (MQ=255)
                                                                                                                   tCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCCTCGCCGGGCTGTTCGTCTTTTTCATCATGc                  >  2:3454782/1‑140 (MQ=255)
                                                                                                                    cGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCg                 >  2:5141144/1‑140 (MQ=255)
                                                                                                                         tatCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCaa            >  1:7944691/1‑140 (MQ=255)
                                                                                                                             gAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATggg        <  2:8719549/140‑1 (MQ=255)
                                                                                                                             gAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATggg        <  1:1039163/140‑1 (MQ=255)
                                                                                                                                  cATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGaaa   >  2:8158293/1‑140 (MQ=255)
                                                                                                                                           ||                                                                                                                                    
CATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAAT  >  NZ_CP025268/3982733‑3983005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑