BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	4,331,820	(G)_7→6	pseudogene (473/817 nt)	rdcB →	YjcZ‑like family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	4,331,814	0	G	.	98.0%	2752.5 / 35.3	594	pseudogene (467/817 nt)	rdcB	YjcZ‑like family protein
Reads supporting (aligned to +/- strand): ref base G (3/9); new base . (254/325); total (257/337)
Fisher's exact test for biased strand distribution p-value = 2.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 619 total aligned reads displayed.

AAAAGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGTCACTATTACC  >  NZ_CP025268/4331676‑4331949

 aaaGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTggg                                                                                                                                       <  2:4409946/140‑1 (MQ=255)

   aGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTggggg                                                                                                                                     >  2:7352709/1‑140 (MQ=255)

   aGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTggggg                                                                                                                                     >  2:6284175/1‑140 (MQ=255)

     gCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTGTATCGTTCTGGAGGAGTTGCGCTggggggc                                                                                                                                   >  1:6801561/1‑139 (MQ=255)

      cGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTggggggca                                                                                                                                  >  1:4990242/1‑138 (MQ=255)

          accGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTATTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTg                                                                                                                             <  2:8892628/138‑1 (MQ=255)

          aaCGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTg                                                                                                                             >  2:577239/1‑140 (MQ=255)

               aaCGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGAGCT‑GGGGGGCATTTGTCGAt                                                                                                                        >  2:521253/1‑140 (MQ=255)

                  gAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGAtgcc                                                                                                                     <  2:2259902/140‑1 (MQ=255)

                   aGGCCTACGGGTGCAGGCAGGTGCTCAGCGCGCGCACAGTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGAtgcct                                                                                                                    <  2:7448830/140‑1 (MQ=255)

                       cTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCg                                                                                                                <  2:2015330/140‑1 (MQ=255)

                         aCTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTc                                                                                                              >  2:1032042/1‑140 (MQ=255)

                             gTGCAGGCAGGTGATCAGCGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTggg                                                                                                          <  1:7641469/140‑1 (MQ=255)

                               gcaggcagGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGaa                                                                                                        >  2:1830601/1‑140 (MQ=255)

                               gcaggcagGGGATCAGAGCGAGCACATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGTGGGCATTTGGCGATGCCTGCCGTCTGGGaa                                                                                                        <  2:1499521/140‑1 (MQ=255)

                                caggcaggTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGTCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTGTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCCTTTGGCGATGCCTGCCGGCTGGGAAg                                                                                                       >  2:9021384/1‑140 (MQ=255)

                                caggcaggTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAg                                                                                                       >  1:1007006/1‑140 (MQ=255)

                                  ggcaggTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGcc                                                                                                     >  1:907778/1‑140 (MQ=255)

                                   gcaggTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGccc                                                                                                    >  2:7623258/1‑140 (MQ=255)

                                        tGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAg                                                                                               <  1:4579090/140‑1 (MQ=255)

                                        tGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTGATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAg                                                                                               >  2:5560742/1‑140 (MQ=255)

                                         gATCAGAGCGAGCAAATTTGTACCCGCTGGCGTGCTGGGGCGTATTACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGc                                                                                              <  2:6773725/140‑1 (MQ=255)

                                             agagCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTg                                                                                          >  2:3737583/1‑140 (MQ=255)

                                               agcgagcAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGc                                                                                        >  2:9315922/1‑140 (MQ=255)

                                               agcgagcAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGc                                                                                        >  2:7768884/1‑140 (MQ=255)

                                                 cgagcAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCgt                                                                                      >  2:6464352/1‑140 (MQ=255)

                                                 cgagcAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCgt                                                                                      >  2:3584946/1‑140 (MQ=255)

                                                 cgagcAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCgt                                                                                      >  1:2489417/1‑140 (MQ=255)

                                                    gcAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCgttgt                                                                                   >  1:2823829/1‑140 (MQ=255)

                                                      aaaTTTGTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGttgttg                                                                                 <  2:9162806/140‑1 (MQ=255)

                                                         tttttACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTg                                                                              >  1:5360026/1‑140 (MQ=255)

                                                         tttttACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGT‑TGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTgg                                                                             >  1:5691230/1‑140 (MQ=255)

                                                         tttttACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGGTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTg                                                                              >  1:3795229/1‑140 (MQ=255)

                                                             tACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTg                                                                          <  1:7582812/140‑1 (MQ=255)

                                                               cccGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGAt                                                                        <  1:2017662/140‑1 (MQ=255)

                                                               cccGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCGGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGAt                                                                        <  2:1672062/140‑1 (MQ=255)

                                                                   cTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTgg                                                                    >  2:8437471/1‑139 (MQ=255)

                                                                       cGTGCAGGGGCGTCTACCGCATGGTCGTTGCCTGGGAGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGTTGGCGTTGTTGCTGGGTGATTTGCTCga                                                                <  2:6172625/140‑1 (MQ=255)

                                                                            tGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAg                                                           <  1:1392556/140‑1 (MQ=255)

                                                                             ggggCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGc                                                          >  1:403198/1‑140 (MQ=255)

                                                                              gggCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCg                                                         >  1:7396245/1‑140 (MQ=255)

                                                                                gCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGac                                                       >  2:4290259/1‑140 (MQ=255)

                                                                                 cGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCTTTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGaca                                                      <  2:3834892/140‑1 (MQ=255)

                                                                                 cGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGaca                                                      <  1:6237881/140‑1 (MQ=255)

                                                                                 cGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGaca                                                      <  1:3303100/140‑1 (MQ=255)

                                                                                   tataACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGAcaca                                                    <  1:843405/140‑1 (MQ=255)

                                                                                   tataACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGAcaca                                                    >  2:4022134/1‑140 (MQ=255)

                                                                                   tataACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGAcaca                                                    <  2:2707304/140‑1 (MQ=255)

                                                                                     taACGCCTGGTCGGTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACacaac                                                  <  2:83231/140‑1 (MQ=255)

                                                                                      aaCGCCTGGTCGTTGCCTTGGCGCTGTTTTATCGTTATGGAGGAGTTGCGCT‑GGGTGGCCTTTGGCGTTGCCTGCAGTGTGGGACGCCCGCAAGGGGTGGCGTTGTTGCTGGGGGTTATGCTCGTGAGAGCGTCacaacg                                                 >  2:7017461/1‑139 (MQ=255)

                                                                                       aCGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACAt                                                <  2:4641893/140‑1 (MQ=255)

                                                                                         gCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCt                                              >  1:6117584/1‑140 (MQ=255)

                                                                                           cTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTgg                                            <  1:877818/140‑1 (MQ=255)

                                                                                           cTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTgg                                            <  2:4862953/140‑1 (MQ=255)

                                                                                           cTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTgg                                            <  1:4678590/140‑1 (MQ=255)

                                                                                             ggTCGTTGCCTGGGCGCTGTTTTCTCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGCGGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCa                                          <  1:6658141/140‑1 (MQ=255)

                                                                                             ggTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCa                                          <  2:416981/140‑1 (MQ=255)

                                                                                             ggTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCa                                          <  1:1280734/140‑1 (MQ=255)

                                                                                             ggTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCa                                          >  2:6635537/1‑140 (MQ=255)

                                                                                             ggTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCa                                          >  1:6516480/1‑140 (MQ=255)

                                                                                             ggTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCa                                          <  1:8051039/140‑1 (MQ=255)

                                                                                             ggTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGGTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCa                                          <  1:2076689/140‑1 (MQ=255)

                                                                                             ggTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCCGGGAAGCCCGCAAGCGGTGGCGTTGTTGGTGGGTGATTTGCTCGAGAAAGCGACCCCACATCGGGcg                                          >  1:8684812/1‑139 (MQ=255)

                                                                                             ggTCGTGGCCTGGGCGCTCTTGAATCGGTCAGGAGGAGTTGCGC‑GGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCa                                          <  2:5736403/140‑1 (MQ=255)

                                                                                             ggTCGTGGCCTGGGCGAATGTTTCTCGTTCTGGAGGATGTGCGCT‑GGGGGGCAGTTGGCGATGCCTGCCGTCTGGGTAGCACGCAAGCGGTGGCTTTGTTGCGGGGTGATGTGCTCGAGAAAGCGACACAACATCGGGCa                                          <  2:7184452/140‑1 (MQ=255)

                                                                                             ggTCGGTGCCGGGGCGCTGATTTCTCGGTCTGGAGTAGTTGCGCT‑TGGGGGCATTTGGCGATGCCTGCCGACTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATGTGAGCGAGAAAGCGCCACAACATCTGGCa                                          <  2:8140525/140‑1 (MQ=255)

                                                                                              gTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCag                                         >  2:3218391/1‑140 (MQ=255)

                                                                                              gTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCag                                         >  1:8157638/1‑140 (MQ=255)

                                                                                               tCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCaga                                        <  1:6956617/140‑1 (MQ=255)

                                                                                               tCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCaga                                        >  2:6595048/1‑140 (MQ=255)

                                                                                                   tGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGt                                    >  1:3548965/1‑140 (MQ=255)

                                                                                                     ccTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACCTCTGGCAGAGAGTAt                                  <  1:1791161/140‑1 (MQ=255)

                                                                                                     ccTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTAt                                  <  1:5212363/140‑1 (MQ=255)

                                                                                                     ccTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTAt                                  <  1:3123270/140‑1 (MQ=255)

                                                                                                     ccTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTAt                                  <  1:9468845/140‑1 (MQ=255)

                                                                                                      cTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATc                                 <  1:7930927/140‑1 (MQ=255)

                                                                                                        gggCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCaa                               >  1:5894471/1‑140 (MQ=255)

                                                                                                        gggCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCaa                               >  2:2946198/1‑140 (MQ=255)

                                                                                                         ggCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCCTTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAAt                              >  2:723317/1‑140 (MQ=255)

                                                                                                         ggCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAAt                              >  1:5495019/1‑140 (MQ=255)

                                                                                                         ggCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAAt                              >  1:2639826/1‑140 (MQ=255)

                                                                                                         ggCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAAt                              <  1:8929448/140‑1 (MQ=255)

                                                                                                         ggCGCTGGTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGACGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGACAGCGACACAACATCTGGCAGAGAGTATCAAt                              >  2:2511283/1‑140 (MQ=255)

                                                                                                          gcgcTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATg                             <  1:4886175/140‑1 (MQ=255)

                                                                                                           cgcTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGc                            <  2:5415503/140‑1 (MQ=255)

                                                                                                             cTGTTTTATCGTTCTGGAGGAGTTGCGC‑GGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGCGACAGCGACACAACATCTGGCAGAGAGTATCAAGGCgg                          <  2:1245733/140‑1 (MQ=255)

                                                                                                                 tttATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGACGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCAcc                      <  2:5255622/140‑1 (MQ=255)

                                                                                                                   tATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGa                    <  2:7941991/140‑1 (MQ=255)

                                                                                                                        ttCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCAcg               <  1:3711006/140‑1 (MQ=255)

                                                                                                                           tGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGt            <  1:4290928/140‑1 (MQ=255)

                                                                                                                           tGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGt            <  1:9313464/140‑1 (MQ=255)

                                                                                                                           tGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGt            <  2:8598019/140‑1 (MQ=255)

                                                                                                                           tGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGt            <  1:4441314/140‑1 (MQ=255)

                                                                                                                           tGGAGGAGGTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGt            <  2:8075517/140‑1 (MQ=255)

                                                                                                                             gaggagTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGTCa          <  1:2988679/140‑1 (MQ=255)

                                                                                                                             gaggagTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGTCa          <  1:7757854/140‑1 (MQ=255)

                                                                                                                                gagTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGTCACTa       <  1:7104373/140‑1 (MQ=255)

                                                                                                                                  gTTGCGCT‑GGGGGGCATTTGGCGATGNCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGTCACTAtt     <  1:6357306/140‑1 (MQ=255)

                                                                                                                                  gTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGTCACTAtt     <  1:9178969/140‑1 (MQ=255)

                                                                                                                                  gTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGTCACTAtt     <  1:2448861/140‑1 (MQ=255)

AAAAGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGTCACTATTACC  >  NZ_CP025268/4331676‑4331949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`