BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	581,197	+G	intergenic (+50/+4)	CXP41_RS24355 → / ← CXP41_RS03085	tail fiber assembly protein/class I SAM‑dependent methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	581,196	1	.	G	98.2%	2321.9 / 27.7	675	intergenic (+49/+5)	CXP41_RS24355/CXP41_RS03085	tail fiber assembly protein/class I SAM‑dependent methyltransferase
Reads supporting (aligned to +/- strand): ref base . (6/6); new base G (338/325); total (344/331)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 686 total aligned reads displayed.

AGCCTGGAAAAAATATCGGGTGTTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑T‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCTTTC  >  NZ_CP025268/581057‑581330

                      ttGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAg                                                                                                                   >  2:4169496/1‑140 (MQ=255)

                          tGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGa                                                                                                               <  1:5986702/140‑1 (MQ=255)

                           gAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGaa                                                                                                              <  1:4628196/140‑1 (MQ=255)

                           gAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCAGTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGCTGCTATTTTTTTCCTGAAGCGGaa                                                                                                              <  1:8507251/140‑1 (MQ=255)

                           gAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGACTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTGTTCCTGTAGCGGaa                                                                                                              <  2:5661194/140‑1 (MQ=255)

                           gAACCGAGTTTATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGaa                                                                                                              <  2:7760186/140‑1 (MQ=255)

                                gtgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGAGGCTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                         <  1:9142309/140‑1 (MQ=255)

                                 tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                        >  2:6382308/1‑140 (MQ=255)

                                 tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                        >  2:5174847/1‑140 (MQ=255)

                                 tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                        >  1:7257568/1‑140 (MQ=255)

                                 tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                        >  1:206119/1‑140 (MQ=255)

                                 tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                        >  2:1771379/1‑140 (MQ=255)

                                 tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                        >  2:8496434/1‑140 (MQ=255)

                                  gtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATta                                                                                                       <  2:3001918/140‑1 (MQ=255)

                                  gtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATta                                                                                                       <  2:3373927/140‑1 (MQ=255)

                                  gtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATta                                                                                                       <  1:5321818/140‑1 (MQ=255)

                                  gtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGGAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATta                                                                                                       <  1:6482/140‑1 (MQ=255)

                                     gATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATtacta                                                                                                    <  1:9007101/140‑1 (MQ=255)

                                      aTACCTCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATtactac                                                                                                   >  2:3840336/1‑140 (MQ=255)

                                        aCATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAg                                                                                                 <  2:2589429/140‑1 (MQ=255)

                                        aCATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAg                                                                                                 <  2:8905438/140‑1 (MQ=255)

                                        aCATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAg                                                                                                 <  2:4957008/140‑1 (MQ=255)

                                           tCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTAc                                                                                              >  1:3661850/1‑140 (MQ=255)

                                           tCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTAc                                                                                              >  1:7851478/1‑140 (MQ=255)

                                           tCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTAc                                                                                              >  1:6323971/1‑140 (MQ=255)

                                           tCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCGTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAACACATAACTACAGTAc                                                                                              >  2:9146452/1‑140 (MQ=255)

                                             aaCTGCACCTGATATTGAGTGGGCTCCGAACCCTGTCAGTGAGTAATCATTGGGATTATGACGGAGCACGTCTTAAGCAAGTACAAGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTAcat                                                                                            <  2:5861305/140‑3 (MQ=255)

                                                tGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGa                                                                                         >  2:1156348/1‑140 (MQ=255)

                                                 gCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGaa                                                                                        >  1:1001321/1‑140 (MQ=255)

                                                 gCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGaa                                                                                        >  2:6741235/1‑140 (MQ=255)

                                                  cACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAAc                                                                                       >  2:8229647/1‑140 (MQ=255)

                                                    cccgaaatTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCAGCAGAACGTCTTAAGCAAGAACATGCTGCTGAT‑G‑GATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCt                                                                                     <  1:2718281/134‑1 (MQ=255)

                                                    ccTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCt                                                                                     <  1:9393585/140‑1 (MQ=255)

                                                    ccTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCt                                                                                     <  2:5420044/140‑1 (MQ=255)

                                                    ccTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCt                                                                                     >  2:2010712/1‑140 (MQ=255)

                                                     cTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCtt                                                                                    <  2:4695711/140‑1 (MQ=255)

                                                     cTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGATATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCtt                                                                                    <  2:321287/140‑1 (MQ=255)

                                                        atatTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGt                                                                                 >  2:8626743/1‑140 (MQ=255)

                                                         tatTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGtt                                                                                >  2:5065635/1‑140 (MQ=255)

                                                          atTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGttt                                                                               >  2:3711602/1‑140 (MQ=255)

                                                            tGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCTGAAAACATTACTACAGTACCTTGAACATTGGTTTTa                                                                             >  1:1386952/1‑140 (MQ=255)

                                                            tGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTa                                                                             >  1:6782507/1‑140 (MQ=255)

                                                               gTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACa                                                                          >  1:9490305/1‑140 (MQ=255)

                                                                tGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACAt                                                                         >  1:1793259/1‑140 (MQ=255)

                                                                  gCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATtc                                                                       >  1:1710966/1‑140 (MQ=255)

                                                                  gCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATtc                                                                       >  2:4098564/1‑140 (MQ=255)

                                                                   ccTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAAAATTACTACAGTACCTTGAACATTGGTTTTAACAttat                                                                      >  2:6184699/1‑138 (MQ=255)

                                                                       cGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGaa                                                                  <  1:98609/140‑1 (MQ=255)

                                                                       agAACCCTGTCAGGGAGTAATCATTGGGATTATTCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑AGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGCACCTTGGTTTTAACCTTCTCGaa                                                                  <  1:9208289/139‑1 (MQ=255)

                                                                        gaccccTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGaaa                                                                 <  2:5990311/137‑1 (MQ=255)

                                                                        gaccccTGCCAGGGAGTAATCCTTGGGATTAAGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGTAG‑GATGCTATTTTTTTCCAGAAGCGGAAAACATTACTACAGTACCTTCACCCTTGGTTTTAACATTCTCGaaa                                                                 <  2:9434691/137‑1 (MQ=255)

                                                                        gAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGaaa                                                                 >  1:2379348/1‑140 (MQ=255)

                                                                        gAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGaaa                                                                 <  1:2144378/140‑1 (MQ=255)

                                                                          aCCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATg                                                               <  1:8663158/140‑1 (MQ=255)

                                                                          aCCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATg                                                               <  1:4996989/140‑1 (MQ=255)

                                                                          aCCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATg                                                               <  1:8102905/140‑1 (MQ=255)

                                                                            ccTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGct                                                             <  1:1505188/140‑1 (MQ=255)

                                                                            ccTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGct                                                             <  1:8843726/140‑1 (MQ=255)

                                                                            ccTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGct                                                             >  1:304002/1‑140 (MQ=255)

                                                                             cTGTCAGGGAGTACTCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGACAACATGACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctc                                                            >  1:6949226/1‑140 (MQ=255)

                                                                              tGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCAGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                           <  2:7109793/140‑1 (MQ=255)

                                                                              tGTCAGGGAGTAATCATTGGGATGATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACGACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctcg                                                           <  2:243492/140‑2 (MQ=255)

                                                                               gTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGaa                                                                                                              <  1:4525614/88‑1 (MQ=255)

                                                                               gTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTg                                                          >  1:3331920/1‑140 (MQ=255)

                                                                                tccgggTGTAATCATTGGGATTATGCCGCTGCAGGTCTTATGCAAGAACTTGGTGG‑GTTGG‑GAGGCTATTTTTGTCCGGACGCGTAAAAATTTAGTACTGTACCTGGAACCTTGGTTTTAACATTCTCGAAATGCTCTga                                                         <  1:3883529/137‑1 (MQ=255)

                                                                                tCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTga                                                         <  1:1579311/140‑1 (MQ=255)

                                                                                tCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTga                                                         <  1:5926744/140‑1 (MQ=255)

                                                                                tCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCCTGAACCTTGGTTTTAACATTCTCGAAATGCTCTga                                                         >  1:768964/1‑140 (MQ=255)

                                                                                 cAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTgag                                                        >  1:4051929/1‑140 (MQ=255)

                                                                                      aGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGtat                                                   >  1:2207565/1‑140 (MQ=255)

                                                                                      aGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGtat                                                   >  1:1198407/1‑140 (MQ=255)

                                                                                      aGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGtat                                                   <  1:7975246/140‑1 (MQ=255)

                                                                                      aGTAAACATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGtat                                                   <  1:1958138/140‑1 (MQ=255)

                                                                                       gTAATCATTGGGATTATGCCGCCGCANGTCATAAGCAAGAACAAGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATNCTCTGAGAGtata                                                  <  1:5541819/140‑1 (MQ=255)

                                                                                       gTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGtata                                                  <  1:294172/140‑1 (MQ=255)

                                                                                       gTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACAGTCTCGAAATGCTCTGAGAGtata                                                  <  1:7424749/140‑1 (MQ=255)

                                                                                        tAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTatat                                                 <  1:5076226/140‑1 (MQ=255)

                                                                                          aTCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGTGAGTATAtgt                                               >  2:9042557/1‑140 (MQ=255)

                                                                                                gggATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAg                                         >  2:812119/1‑140 (MQ=255)

                                                                                                  gATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGcc                                       >  2:2835851/1‑140 (MQ=255)

                                                                                                   aTTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCct                                      >  1:6184783/1‑140 (MQ=255)

                                                                                                     tATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCcttc                                    >  1:8598010/1‑140 (MQ=255)

                                                                                                     tATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCcttc                                    >  2:1561240/1‑140 (MQ=255)

                                                                                                     tATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCcttc                                    >  2:6506613/1‑140 (MQ=255)

                                                                                                      aTGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCcttct                                   >  1:4179974/1‑140 (MQ=255)

                                                                                                      aTGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCcttct                                   >  1:1292449/1‑140 (MQ=255)

                                                                                                      aTGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCcttct                                   >  2:2240343/1‑140 (MQ=255)

                                                                                                           gcagcaCGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGa                              >  1:4788131/1‑140 (MQ=255)

                                                                                                            cagcaCGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGaa                             >  2:6956055/1‑140 (MQ=255)

                                                                                                            cagcaCGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGaa                             >  2:3624373/1‑140 (MQ=255)

                                                                                                            cagcaCGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGaa                             >  1:6592867/1‑140 (MQ=255)

                                                                                                            cagcaCGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGaa                             >  1:4386668/1‑140 (MQ=255)

                                                                                                            cagcaCGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTGCGTCGGaa                             >  1:5751859/1‑140 (MQ=255)

                                                                                                                        aGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATCTGGGTTAAGCCTTCTTCGGAATCTGTTgtgtgt                 >  2:7607374/1‑138 (MQ=255)

                                                                                                                            aGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGaaa             >  2:721352/1‑140 (MQ=255)

                                                                                                                            aGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGaaa             >  2:7975636/1‑140 (MQ=255)

                                                                                                                             gAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAATCTTTACTACAGTACCTTGAACCTTGGTGTTAACATTCTCGAAATGATCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGaaaa            >  2:1385017/1‑140 (MQ=255)

                                                                                                                              aaCATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAg           >  1:8249386/1‑140 (MQ=255)

                                                                                                                              aaCATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAg           <  2:3493381/140‑1 (MQ=255)

                                                                                                                                       c‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCTTTc  >  2:446412/1‑140 (MQ=255)

AGCCTGGAAAAAATATCGGGTGTTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑T‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCTTTC  >  NZ_CP025268/581057‑581330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`