BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	674,649	+T	pseudogene (947/977 nt)	ybeQ ←	SEL1‑like repeat protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	674,649	1	.	T	99.0%	1927.7 / 6.9	502	pseudogene (947/977 nt)	ybeQ	SEL1‑like repeat protein
Reads supporting (aligned to +/- strand): ref base . (4/1); new base T (231/264); total (235/267)
Fisher's exact test for biased strand distribution p-value = 1.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 517 total aligned reads displayed.

TGGTCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTC‑C‑GACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAGGTTATACCAGGCAGTGGCGTCATTGC  >  NZ_CP025268/674510‑674778

      tGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTg                                                                                                                              >  1:4980763/1‑140 (MQ=255)

        aCCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTgactgac                                                                                                                            <  1:781058/140‑1 (MQ=255)

        aCCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTgactgac                                                                                                                            <  1:1564545/140‑1 (MQ=255)

        aCCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTgactgac                                                                                                                            <  1:7162419/140‑1 (MQ=255)

        aCCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAAGTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTgactgac                                                                                                                            <  1:9550334/140‑1 (MQ=255)

            gTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACAtct                                                                                                                        >  2:7089804/1‑140 (MQ=255)

             ttCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACAtctc                                                                                                                       <  1:1811646/140‑1 (MQ=255)

             ttCCAGAGACGCCCGTTTGAGCCGTAATAGTATGATTTCGCACAGCAATAAAGAGAATATGGGACGAATGAAATTACCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCAT‑GACTGTCAtctc                                                                                                                       >  1:277215/1‑140 (MQ=255)

                 agagACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGt                                                                                                                   >  2:2638253/1‑140 (MQ=255)

                  gagaCGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGtt                                                                                                                  >  1:340237/1‑140 (MQ=255)

                  gagaCGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGtt                                                                                                                  >  1:9207005/1‑140 (MQ=255)

                     aCGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCg                                                                                                               <  2:422349/140‑1 (MQ=255)

                     aCGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCg                                                                                                               >  1:242692/1‑140 (MQ=255)

                      cGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGAAATAAAGAGAATATGGGCCGAATGAAATTCACCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCAT‑GACTGAAATCTCTGGTTCCgg                                                                                                              >  1:4505204/1‑140 (MQ=255)

                       gCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTGTCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGa                                                                                                             >  2:8826094/1‑140 (MQ=255)

                       gCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCTCGAATTTC‑CTGACTGACATCTCTGGTTCCGGa                                                                                                             >  1:3145596/1‑140 (MQ=255)

                       gCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGa                                                                                                             >  1:2515561/1‑140 (MQ=255)

                       gCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGa                                                                                                             >  1:2980385/1‑140 (MQ=255)

                       gCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGa                                                                                                             >  1:5252054/1‑140 (MQ=255)

                       gCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGa                                                                                                             >  2:3534722/1‑140 (MQ=255)

                       gCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCGACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGGTCCGGa                                                                                                             >  1:2039/1‑140 (MQ=255)

                        cccGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGAt                                                                                                            >  2:2876078/1‑140 (MQ=255)

                          cGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGa                                                                                                          >  2:9011725/1‑140 (MQ=255)

                               gAGCCGTAATAGTCTGATTTCGCACAGCAATACAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGaa                                                                                                     <  1:2225709/140‑1 (MQ=255)

                               gAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGaa                                                                                                     <  2:3440509/140‑1 (MQ=255)

                               gAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGaa                                                                                                     <  2:5865847/140‑1 (MQ=255)

                               gAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGaa                                                                                                     <  1:8499308/140‑1 (MQ=255)

                                    gTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTa                                                                                                <  2:5729493/140‑1 (MQ=255)

                                     tAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTAc                                                                                               <  2:2462970/140‑1 (MQ=255)

                                       aTAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGa                                                                                             <  1:3534722/140‑1 (MQ=255)

                                       aTAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGa                                                                                             >  1:4107238/1‑140 (MQ=255)

                                         aGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTAGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATa                                                                                           <  1:7948607/140‑1 (MQ=255)

                                          gTCTGATTTCGCACAGCAATAAAGAGACTATGGGCCGAATGAAAGTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATAc                                                                                          <  2:8361181/140‑1 (MQ=255)

                                            cTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAg                                                                                        >  2:2116427/1‑140 (MQ=255)

                                               aTTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTc                                                                                     >  2:8000289/1‑140 (MQ=255)

                                               aTTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTc                                                                                     >  1:2460331/1‑140 (MQ=255)

                                               aTTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACACTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTc                                                                                     >  1:6849780/1‑140 (MQ=255)

                                                tttCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATT‑CCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCga                                                                                   <  1:1002465/140‑1 (MQ=255)

                                                     cacaGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGc                                                                               <  1:7694903/140‑1 (MQ=255)

                                                          cAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTc                                                                          >  2:4624668/1‑140 (MQ=255)

                                                             tAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGga                                                                       >  1:4664780/1‑140 (MQ=255)

                                                             tAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGga                                                                       >  2:3810657/1‑140 (MQ=255)

                                                                  agaATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTg                                                                                                       <  1:2904566/103‑1 (MQ=255)

                                                                   gaATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGa                                                                 <  1:3843794/140‑1 (MQ=255)

                                                                    aaTATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACATTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGAc                                                                <  1:5965196/140‑1 (MQ=255)

                                                                    aaTATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGAc                                                                >  1:2696862/1‑140 (MQ=255)

                                                                    aaTATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGAc                                                                >  2:1318303/1‑140 (MQ=255)

                                                                    aaTATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGAc                                                                >  1:3379108/1‑140 (MQ=255)

                                                                    aaTATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGAc                                                                >  2:485826/1‑140 (MQ=255)

                                                                     atatGGGCCGACCGCAAGTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGAGGACTGACCTTTACGATACCGGTCGCGAGCCTGTCGGAGATCGACa                                                               <  2:1704478/140‑1 (MQ=255)

                                                                     atatGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACa                                                               <  1:8378971/140‑1 (MQ=255)

                                                                      tatGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAg                                                              <  1:3198642/140‑1 (MQ=255)

                                                                      tatGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAg                                                              <  1:3648777/140‑1 (MQ=255)

                                                                            ccGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTcttc                                                        >  1:8693427/1‑140 (MQ=255)

                                                                            ccGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTcttc                                                        >  1:614953/1‑140 (MQ=255)

                                                                            ccGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTcttc                                                        <  2:6006576/140‑1 (MQ=255)

                                                                             cGAATGAAACTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTcttcc                                                       <  1:6646394/140‑1 (MQ=255)

                                                                               aaTGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTcttcctt                                                     <  1:8185187/140‑1 (MQ=255)

                                                                               aaTGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTcttcctt                                                     <  2:5576888/140‑1 (MQ=255)

                                                                               aaTGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTcttcctt                                                     >  2:2320095/1‑140 (MQ=255)

                                                                                 tGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCCGTCGGAGATCGACAGGTCTTCCTtct                                                   <  1:874928/140‑1 (MQ=255)

                                                                                    aaTTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCg                                                <  1:1926707/140‑1 (MQ=255)

                                                                                    aaTTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCg                                                <  2:7727771/140‑1 (MQ=255)

                                                                                    aaTTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATGTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCg                                                <  2:6993763/140‑1 (MQ=255)

                                                                                     aTTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCGTCCTTCTCCgg                                               >  1:6554209/1‑139 (MQ=255)

                                                                                       tCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAg                                             <  1:6294842/140‑1 (MQ=255)

                                                                                       tCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAg                                             <  1:5051637/140‑1 (MQ=255)

                                                                                         cccGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGGCTTCCTTCTCCGTAGTg                                           >  1:549253/1‑140 (MQ=255)

                                                                                           cGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGAt                                         <  1:4252118/140‑1 (MQ=255)

                                                                                           cGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGAt                                         <  1:1411646/140‑1 (MQ=255)

                                                                                           cGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGAt                                         <  2:5010531/140‑1 (MQ=255)

                                                                                              cccTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACa                                      <  2:4684219/140‑1 (MQ=255)

                                                                                              cccTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACa                                      >  2:3933536/1‑140 (MQ=255)

                                                                                                cTTTTTTTTATCTCTACAGTACATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGTAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATa                                    <  2:7343911/140‑1 (MQ=255)

                                                                                                cTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTTGTTCCGGATGACTGATCTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATa                                    <  2:1931923/140‑1 (MQ=255)

                                                                                                cTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATa                                    <  2:6567212/140‑1 (MQ=255)

                                                                                                   gTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGAGCGACAGGTCTTCCTTCTCCGTAGTGATACATAATg                                 >  1:479277/1‑140 (MQ=255)

                                                                                                    tttttATCTCTACCGTAAATCTTCAGTCTCACGAATTTC‑CGGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATgg                                <  1:8543039/140‑1 (MQ=255)

                                                                                                    tttttATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATgg                                <  1:4547413/140‑1 (MQ=255)

                                                                                                     ttttATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGc                               <  1:1243048/140‑1 (MQ=255)

                                                                                                     ttttATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGc                               <  1:1757868/140‑1 (MQ=255)

                                                                                                        tATCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTTGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGcccg                            >  2:7597907/1‑138 (MQ=255)

                                                                                                         aTCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCTGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAgg                           >  2:2834593/1‑140 (MQ=255)

                                                                                                         aTCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAgg                           <  1:1694500/140‑1 (MQ=255)

                                                                                                         aTCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAgg                           <  2:5169754/140‑1 (MQ=255)

                                                                                                         aTCTCTACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAgg                           <  1:2367993/140‑1 (MQ=255)

                                                                                                              tACAGTAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAGGTtata                      >  1:5636042/1‑140 (MQ=255)

                                                                                                                   tAAATCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCTGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAGGTTATACCAgg                 >  2:6368988/1‑140 (MQ=255)

                                                                                                                     aaTCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGaa                                                                                                     >  2:8516434/1‑54 (MQ=255)

                                                                                                                      aTCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAGGTTATACCAGGCAg              <  1:3933536/140‑1 (MQ=255)

                                                                                                                      aTCTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAGGTTATACCAGGCAg              >  1:8894337/1‑140 (MQ=255)

                                                                                                                      aTCTACAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAGGTTATACCAGGCAg              <  1:5172286/140‑1 (MQ=255)

                                                                                                                        cTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAGGTTATACCAGGCAGTg            >  1:7331496/1‑140 (MQ=255)

                                                                                                                        cTTCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAGGTTATACAAGGCAGTg            >  1:6380672/1‑140 (MQ=255)

                                                                                                                         ttCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACATTACGAGACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAGGTTATACCAGGCAGTgg           <  1:5783799/140‑1 (MQ=255)

                                                                                                                          tCAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATa                                    >  1:7016733/1‑114 (MQ=255)

                                                                                                                           cAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAGGTTATACCAGGCAGTGGCg         >  2:9384845/1‑140 (MQ=255)

                                                                                                                           cAGTCTCACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAGGTTATACCAGGCAGTGGCg         >  2:3047371/1‑140 (MQ=255)

                                                                                                                                tcACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAGGTTATACCAGGCAGTGGCGTCAtt    >  1:9421365/1‑140 (MQ=255)

                                                                                                                                 cACGAATTTC‑CTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAGGTTATACCAGGCAGTGGCGTCATTg   >  2:4451861/1‑140 (MQ=255)

TGGTCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTC‑C‑GACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAGGTTATACCAGGCAGTGGCGTCATTGC  >  NZ_CP025268/674510‑674778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`