BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,629,176	(T)_7→8	pseudogene (141/392 nt)	ydeI ←	YdeI family stress tolerance OB fold protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,629,169	1	.	T	96.3%	1003.3 / 24.8	269	pseudogene (148/392 nt)	ydeI	YdeI family stress tolerance OB fold protein
Reads supporting (aligned to +/- strand): ref base . (1/9); new base T (64/195); total (65/204)
Fisher's exact test for biased strand distribution p-value = 4.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

Only 100 of 279 total aligned reads displayed.

CGGCCGCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATACAA  >  NZ_CP025268/1629032‑1629300

   ccGCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑‑t‑tttt                                                                                                                                   >  2:8325192/1‑140 (MQ=255)

        ggAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑‑t‑tttt‑t‑ttgc                                                                                                                            <  2:5417695/140‑4 (MQ=255)

        ggAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑‑t‑tttt‑t‑ttgc                                                                                                                            <  2:3787548/140‑4 (MQ=255)

             aaCGACATTAATTTCCCCGCACGTCTCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑Gcatcat                                                                                                                       <  1:4489143/140‑1 (MQ=255)

             aaCGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACAGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑Gcatcat                                                                                                                       <  1:3428826/140‑1 (MQ=255)

                   aTTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTc                                                                                                                 >  1:6265885/1‑140 (MQ=255)

                     tAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCcg                                                                                                               >  1:8229889/1‑140 (MQ=255)

                       aTTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCcgcg                                                                                                             <  1:8061664/140‑1 (MQ=255)

                        tttCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGa                                                                                                            <  1:6689895/140‑1 (MQ=255)

                        tttCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGa                                                                                                            <  2:8646413/140‑1 (MQ=255)

                        cttCACCGCTCTAATCGCGAAAAACGAAACGGTCCACTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGa                                                                                                            <  2:2144979/139‑1 (MQ=255)

                         ttCACCGCTCTTATCGCGAAAAACGGAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGAt                                                                                                           <  1:8177784/140‑1 (MQ=255)

                           cACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAg                                                                                                         <  2:3691075/140‑1 (MQ=255)

                           cACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAg                                                                                                         <  1:4244339/140‑1 (MQ=255)

                           cACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAg                                                                                                         <  2:3505972/140‑1 (MQ=255)

                            aCCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGc                                                                                                        >  2:4945364/1‑140 (MQ=255)

                            aCCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGc                                                                                                        >  1:2615401/1‑140 (MQ=255)

                             ccGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGcc                                                                                                       <  1:2964568/140‑1 (MQ=255)

                             ccGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGcc                                                                                                       <  1:3470272/140‑1 (MQ=255)

                             ccGCTCTGATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGcc                                                                                                       <  1:8768618/140‑1 (MQ=255)

                              cGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAAGCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCa                                                                                                      >  1:8158854/1‑140 (MQ=255)

                                ctctTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGGAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCAtc                                                                                                    <  2:4992674/140‑1 (MQ=255)

                                ctctTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCAtc                                                                                                    <  2:9164678/140‑1 (MQ=255)

                                ctctTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCAtc                                                                                                    <  1:5691644/140‑1 (MQ=255)

                                  ctTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAACTCAAATTACCGCGTAGTGAAACCGCAGCGCCAACGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCAtctt                                                                                                  <  1:2660727/140‑1 (MQ=255)

                                  ctTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCAtctt                                                                                                  <  1:5110438/140‑1 (MQ=255)

                                     aTCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTa                                                                                               >  1:9146548/1‑140 (MQ=255)

                                        gcgAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTg                                                                                            <  2:6437169/140‑1 (MQ=255)

                                          gAAAAACGTAACGGTCCTCTCCTTTGTGAGAACTCAACTTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTATTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCa                                                                                          <  1:2004956/140‑1 (MQ=255)

                                          gAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCa                                                                                          <  1:4536435/140‑1 (MQ=255)

                                           aaaaaCGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCAt                                                                                         <  2:1268994/140‑1 (MQ=255)

                                           aaaaaCGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCAt                                                                                         >  2:6893142/1‑140 (MQ=255)

                                           aaaaaCGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCAt                                                                                         >  2:9554768/1‑140 (MQ=255)

                                           aaaaaCGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCAt                                                                                         <  1:4865729/140‑1 (MQ=255)

                                           aaaaaCGTAACGCTCCTCTCCTGTGTGAGAAATCAAATTACAGAGTAGTGAAACCGAAGCGCCATAGGTCATGGGTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCGTCTATTTCAt                                                                                         <  2:1718918/140‑1 (MQ=255)

                                             aaaCGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGa                                                                                       >  1:1727519/1‑140 (MQ=255)

                                                cGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAggc                                                                                    >  1:2243825/1‑140 (MQ=255)

                                                cGTAACGGTCCTCTCCTGTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAggc                                                                                    <  1:8129688/140‑1 (MQ=255)

                                                  tAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAggcgg                                                                                  <  2:7967463/140‑1 (MQ=255)

                                                    aCGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGgcggcg                                                                                <  2:6771163/140‑1 (MQ=255)

                                                     cGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGcggcgg                                                                               <  2:4413406/140‑1 (MQ=255)

                                                          ctctCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGgcg                                                                          <  1:691690/140‑1 (MQ=255)

                                                          ctctCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGgcg                                                                          >  1:2506444/1‑140 (MQ=255)

                                                            ctcCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCt                                                                        <  1:8478690/140‑1 (MQ=255)

                                                            ctcCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCt                                                                        <  2:3945479/140‑1 (MQ=255)

                                                                 ttGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATc                                                                   <  1:2872188/140‑1 (MQ=255)

                                                                  tgtgAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCATTT‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCAGGAGGCGGCGGTGGCGCTGCATc                                                                   >  2:5771181/1‑140 (MQ=255)

                                                                  tgtgAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATcc                                                                  >  2:4764449/1‑140 (MQ=255)

                                                                  tgtgAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATcc                                                                  >  1:841526/1‑140 (MQ=255)

                                                                  tgtgAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATcc                                                                  >  2:6359488/1‑140 (MQ=255)

                                                                  tgtgAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATcc                                                                  >  1:9315202/1‑140 (MQ=255)

                                                                  tgtgAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATcc                                                                  >  1:999518/1‑140 (MQ=255)

                                                                   gtgAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCt                                                                 <  2:9411638/140‑1 (MQ=255)

                                                                           tCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTaaa                                                         <  2:5200428/140‑1 (MQ=255)

                                                                           tCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTaaa                                                         <  2:3139604/140‑1 (MQ=255)

                                                                           tCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑‑T‑TTTT‑TGT‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTaaa                                                         <  1:6421325/140‑1 (MQ=255)

                                                                            cAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAAc                                                        >  2:4357059/1‑140 (MQ=255)

                                                                              aaTGACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCg                                                      <  2:6715573/140‑1 (MQ=255)

                                                                                 tACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑‑T‑TTTT‑TGT‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGccc                                                   <  2:2411274/140‑1 (MQ=255)

                                                                                  aCCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCt                                                  >  2:7025346/1‑140 (MQ=255)

                                                                                     gcgTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTCTTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGt                                               <  1:7587048/140‑1 (MQ=255)

                                                                                     gcgTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGt                                               <  1:8845091/140‑1 (MQ=255)

                                                                                     gcgTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGt                                               <  1:1458739/140‑1 (MQ=255)

                                                                                        tAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCAt                                            >  2:2007719/1‑140 (MQ=255)

                                                                                         aGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATc                                           >  1:241961/1‑140 (MQ=255)

                                                                                         aGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATc                                           <  2:5676607/140‑1 (MQ=255)

                                                                                             aaaCCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGcc                                       <  2:2559131/140‑1 (MQ=255)

                                                                                             aaaCCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGcc                                       <  1:7342588/140‑1 (MQ=255)

                                                                                             aaaCCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGcc                                       <  2:2559196/140‑1 (MQ=255)

                                                                                             aaaCCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGcc                                       <  2:2293069/140‑1 (MQ=255)

                                                                                              caCCGACGCCCCCTCGTGCATGGATTTTGCGAAATCAACGGTCA‑‑T‑TTTTAT‑T‑GCAACATCGGTTCCGCGATAGCCATCTTCTCTTGCATGAGGCGGCGGTGGCGCTGAATCCTGTTTTAAACCGCCCTGGTCATCTGCCa                                      <  2:6355927/139‑1 (MQ=255)

                                                                                              aaCCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCa                                      <  2:7177124/140‑1 (MQ=255)

                                                                                                   aaGCGCCATCGTGCATGGTTTTTGCGAAATCCACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCa                                 <  1:5045087/140‑1 (MQ=255)

                                                                                                   aaGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCa                                 <  1:4054221/140‑1 (MQ=255)

                                                                                                   aaGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGAGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCa                                 <  1:1058905/140‑1 (MQ=255)

                                                                                                   aaGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATAGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCa                                 >  1:7680263/1‑140 (MQ=255)

                                                                                                   aaGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑‑T‑TTTT‑T‑TAGCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGct                                 >  2:2877184/1‑139 (MQ=255)

                                                                                                    aGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCAt                                <  1:7025346/140‑1 (MQ=255)

                                                                                                    aGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCAt                                <  1:5839071/140‑1 (MQ=255)

                                                                                                    aGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCAt                                <  1:5446274/140‑1 (MQ=255)

                                                                                                     gcgcCATCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATa                               >  2:871704/1‑140 (MQ=255)

                                                                                                           tCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCAt                         <  1:1127004/140‑1 (MQ=255)

                                                                                                           tCGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCAt                         <  1:3946962/140‑1 (MQ=255)

                                                                                                            cGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATg                        <  2:3680961/140‑1 (MQ=255)

                                                                                                            cGTGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATg                        >  1:5985949/1‑140 (MQ=255)

                                                                                                              tGCATGGTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGAc                      <  2:3369407/140‑1 (MQ=255)

                                                                                                                   ggTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaa                 >  2:3829573/1‑140 (MQ=255)

                                                                                                                   ggTTTTTGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaa                 >  1:5205218/1‑140 (MQ=255)

                                                                                                                     tttttGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa               <  1:2728543/140‑1 (MQ=255)

                                                                                                                     tttttGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa               <  1:1276481/140‑1 (MQ=255)

                                                                                                                     tttttGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGaaaa               <  1:3640982/140‑1 (MQ=255)

                                                                                                                     cagttGCGCAATCAACGGTCA‑‑TATTTA‑T‑A‑GCATCACCGGTTCCGCGATACCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCAGTTTTAAACCGCCCTGGGCATCTGCCAACGCATAAGGCATGACAAGaaaa               <  2:7321033/137‑1 (MQ=255)

                                                                                                                         tGCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCTTCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCGGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTg           <  2:7693881/140‑1 (MQ=255)

                                                                                                                          gCGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGc          <  2:4116702/140‑1 (MQ=255)

                                                                                                                           cGAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCt         <  1:8574878/140‑1 (MQ=255)

                                                                                                                            gAAATCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTa        <  2:2964472/140‑1 (MQ=255)

                                                                                                                               aTCAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATa     >  2:5888961/1‑140 (MQ=255)

                                                                                                                                 cAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATACa   <  1:5329058/140‑1 (MQ=255)

                                                                                                                                 cAACGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATACa   <  1:7792265/140‑1 (MQ=255)

                                                                                                                                  aaCGGTCAT‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCTCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATACaa  <  2:4822196/140‑1 (MQ=255)

CGGCCGCAGGAATAACGACATTAATTTCACCGCTCTTATCGCGAAAAACGTAACGGTCCTCTCCTTTGTGAGAAATCAAATTACCGCGTAGTGAAACCGAAGCGCCATCGTGCATGGTTTTTGCGAAATCAACGGTCA‑‑T‑TTTT‑T‑T‑GCATCATCGGTTCCGCGATAGCCATCTTCTATTGCATGAGGCGGCGGTGGCGCTGCATCCTGTTTTAAACCGCCCTGGTCATCTGCCAACGCATAAGGCATGACAAGAAAACTTGCTAATACAA  >  NZ_CP025268/1629032‑1629300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`